EN ISO 22959:2009

Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons by on-line donor-acceptor complex chromatography and HPLC with fluorescence detection (ISO 22959:2009)

ISO 22959:2009 specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.
The method has been validated for coconut, olive, sunflower, and soybean oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters.
The lowest level of quantification for the PAHs is 0,1 µg/kg. The lowest possible amount of each PAH which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1 µg/kg to 3,5 µg/kg for each individual PAH. For samples containing (light) PAH contents > 3,5 µg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However, ranges exceeding 3,5 µg/kg have not been validated.
PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo[e]pyrene, benzo[a]pyrene, perylene, benzo[ghi]perylene, anthanthrene, dibenzo[a,h]anthracene, coronene, indeno[1,2,3-cd]pyrene, benzo[a]fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene.

Tierische und pflanzliche Fette und Öle - Bestimmung polycyclischer aromatischer Kohlenwasserstoffe durch gekoppelte Donor-Akzeptor-Komplex-Chromatographie und HPLC mit Fluoreszenzdetektion (ISO 22959:2009)

Diese Internationale Norm legt ein hochleistungs-flüssigchromatographisches (HPLC, en.: high performance liquid chromatographic) Verfahren zur Bestimmung von polycyclischen aromatischen Kohlenwasserstoffen (PAK) in Speise¬fetten und -ölen.
Das Verfahren wurde bereits für Kokosnuss- (CN, en.: coconut), Oliven- (OV, en.: olive), Sonnenblumen- (SF, en.: sunflower) und Sojabohnenöl (BO, en.: Soyabean) validiert und ist möglicherweise anwendbar auf andere Öle, abhängig von der Bestimmung geeig¬neter Parameter.
Das geringste Niveau der Quantifizierung von PAKs beträgt 0,1 µg/kg. Die geringste mögliche Menge jedes PAK, die aus dem Basislinienrauschen differenziert werden kann, ist nicht ermittelt worden. Der validierte Konzentrationsbereich des Verfahrens liegt zwischen 0,1 µg/kg und 3,5 µg/kg für jeden einzelnen PAK. Für Proben, die (leichte) PAK-Gehalte > 3,5 µg/kg haben, ist eine Verdünnung möglich, um die Gehalte in einen validierten Bereich zu bringen. Eine Anpassung des Bereiches der Kalibrierkurven ist ebenfalls möglich. Bereiche oberhalb 3,5 µg/kg sind jedoch nicht validiert.
PAK, die mit diesem Verfahren bestimmt werden können sind: Anthracen, Phenanthren, Fluoranthen, Pyren, Chrysen, Benzo[a]anthracen, Benzo[e]pyren, Benzo[a]pyren, Perylen, Benzo[g,h,i]perylen, Anthanthren, Dibenzo[a,h]anthracen, Coronen, Indeno[1,2,3-cd]pyren, Benzo[a]fluoranthen, Benzo[b]fluoranthen, Benzo[k]fluoran¬then.

Corps gras d'origines animale et végétale - Détermination de la teneur en hydrocarbures aromatiques polycycliques par chromatographie de complexe donneur-accepteur et CLHP avec détection par fluorescence (ISO 22959:2009)

L'ISO 22959:2009 spécifie un mode opératoire de chromatographie liquide à haute performance (CLHP) permettant de déterminer la teneur en hydrocarbures aromatiques polycycliques (HAP) dans les corps gras comestibles.
La méthode a été validée pour l'huile de coco (CN), l'huile d'olive (OV), l'huile de tournesol (SF) et l'huile de soja (BO) et peut être applicable à d'autres huiles après détermination des paramètres appropriés.
La plus faible limite de quantification pour l'ensemble des HAP est fixée à 0,1 μg/kg. La plus petite quantité de chaque HAP qu'il est possible de distinguer des signaux parasites n'a pas été déterminée. La plage de concentration validée pour la méthode est comprise entre 0,1 μg/kg et 3,5 μg/kg pour chaque HAP. Dans le cas de teneurs en HAP (légers) supérieures à 3,5 μg/kg, il est possible de diluer les échantillons afin d'amener la teneur dans la plage validée. Il est également possible de régler le domaine de travail des courbes d'étalonnage. Cependant, les domaines supérieurs à 3,5 μg/kg n'ont pas été validés.
La présente méthode permet de déterminer les HAP suivants: anthracène, phénanthrène, fluoranthène, pyrène, chrysène, benz[a]anthracène, benzo[e]pyrène, benzo[a]pyrène, pérylène, benzo[ghi]pérylène, anthanthrène, dibenz[a,h]anthracène, coronène, indéno[1,2,3-cd]pyrène, benzo[a]fluoranthène, benzo[b]fluoranthène, benzo[k]fluoranthène.

Živalske in rastlinske maščobe in olja - Določevanje policikličnih aromatskih ogljikovodikov z neposredno kompleksno "donor-akceptor" kromatografijo in HPLC s fluorescenčno detekcijo (ISO 22959:2009)

General Information

• Status: Published
• Publication Date: 14-May-2009
• Withdrawal Date: 29-Nov-2009
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: ISO/TC 34 - ISO/TC 34
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 15-May-2009
• Completion Date: 15-May-2009
• Ref Project: SIST EN ISO 22959:2009 - Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons by on-line donor acceptor complex chromatography and HPLC with fluorescence detection (ISO 22959:2009)

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 22959:2009
01-september-2009
äLYDOVNHLQUDVWOLQVNHPDãþREHLQROMD'RORþHYDQMHSROLFLNOLþQLKDURPDWVNLK
RJOMLNRYRGLNRY]QHSRVUHGQRNRPSOHNVQRGRQRUDNFHSWRUNURPDWRJUDILMRLQ
+3/&VIOXRUHVFHQþQRGHWHNFLMR,62
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons
by on-line donor acceptor complex chromatography and HPLC with fluorescence
detection (ISO 22959:2009)
Tierische und pflanzliche Fette und Öle - Bestimmung polycyclischer aromatischer
Kohlenwasserstoffe durch direkte Donor-Akzeptor-Komplex-Chromatographie und HPLC
mit Fluoreszenzdetektion (ISO 22959:2009)
Corps gras d'origines animale et végétale - Détermination de la teneur en hydrocarbures
aromatiques polycycliques par chromatographie de complexe donneur -accepteur et
CLHP avec détection par fluorescence (ISO 22959:2009)
Ta slovenski standard je istoveten z: EN ISO 22959:2009
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 22959:2009 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 22959:2009

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SIST EN ISO 22959:2009
EUROPEAN STANDARD
EN ISO 22959
NORME EUROPÉENNE
EUROPÄISCHE NORM
May 2009
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Determination of polycyclic
aromatic hydrocarbons by on-line donor-acceptor complex
chromatography and HPLC with fluorescence detection (ISO
22959:2009)
Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung
de la teneur en hydrocarbures aromatiques polycycliques polycyclischer aromatischer Kohlenwasserstoffe durch
par chromatographie de complexe donneur-accepteur et gekoppelte Donor-Akzeptor-Komplex-Chromatographie und
CLHP avec détection par fluorescence (ISO 22959:2009) HPLC mit Fluoreszenzdetektion (ISO 22959:2009)
This European Standard was approved by CEN on 1 May 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22959:2009: E
worldwide for CEN national Members.

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SIST EN ISO 22959:2009
EN ISO 22959:2009 (E)
Contents Page
Foreword .3

2

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SIST EN ISO 22959:2009
EN ISO 22959:2009 (E)
Foreword
This document (EN ISO 22959:2009) has been prepared by Technical Committee ISO/TC 34 "Agricultural
food products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by November 2009, and conflicting national standards shall be withdrawn
at the latest by November 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Sl
...