EN ISO 11702:2009
(Main)Animal and vegetable fats and oils - Enzymatic determination of total sterols content (ISO 11702:2009)
Animal and vegetable fats and oils - Enzymatic determination of total sterols content (ISO 11702:2009)
ISO 11702:2009 specifies a method for the quantitative determination of the total sterols content by means of an enzymatic staining test. The method is applicable to free and esterified sterols in animal and vegetable fats and oils, fatty foods and related products. The determination is applicable to sample quantities of 1 g to 2 g of fat.
The method is not applicable to dark coloured fats and oils, e.g. crude palm oil. The enzyme is not specific for cholesterol, but also oxidizes other 3-hydroxysterols. The method has not been tested for products fortified with sterols at higher levels.
Tierische und pflanzliche Fette und Öle - Enzymatische Bestimmung des Gesamtsterin-Gehaltes (ISO 11702:2009)
Diese Internationale Norm legt ein Verfahren zur quantitativen Bestimmung des Gesamtsterin-Gehaltes durch einen enzymatischen Farbtest fest. Das Verfahren ist anwendbar auf freie und veresterte Sterine in tierischen und pflanzlichen Fetten und Ölen, fetthaltigen Lebensmitteln und verwandten Erzeugnissen. Die Bestimmung gilt für Probenmengen von 1 g bis 2 g Fett.
Das Verfahren ist nicht auf dunkel gefärbte Fette und Ölen, wie z. B. rohes Palmöl, anwendbar. Das Enzym ist nicht spezifisch für Cholesterin, sondern es werden auch andere 3 Hydroxysterine oxidiert. Das Verfahren wurde nicht an Produkten geprüft, die mit höheren Konzentrationen von Sterinen angereichert waren.
ANMERKUNG Das Verfahren ist der IUPAC Methode 2.404 [8] und der DGF Einheitsmethode F III 2 (91) [7] technisch gleichwertig.
Corps gras d'origines animale et végétale - Détermination enzymatique de la teneur en stérols totaux (ISO 11702:2009)
L'ISO 11702:2009 spécifie une méthode pour la détermination quantitative de la teneur en stérols totaux à l'aide d'un essai de coloration enzymatique. La méthode s'applique aux stérols libres et estérifiés, contenus dans les corps gras d'origines animale et végétale, dans les aliments gras et les produits connexes. La détermination s'applique aux quantités d'échantillon de 1 g à 2 g de matière grasse.
Cette méthode ne s'applique pas aux corps gras de couleur sombre comme l'huile de palme brute. L'enzyme n'est pas spécifique du cholestérol et oxyde également d'autres 3‑hydroxystérols. La méthode n'a pas été testée pour des produits enrichis avec des stérols à des niveaux plus élevés.
Rastlinske in živalske maščobe in olja - Encimsko določevanje celotnih sterolov (ISO 11702:2009)
Ta mednarodni standard določa metodo za količinsko določevanje celotnih sterolov z encimskim preskusom obarvanja. Metoda velja za proste in esterificirane sterole v živalskih in rastlinskih maščobah in oljih, maščobnih živilih in sorodnih proizvodih. Določevanje se lahko uporablja pri vzorcih z 1 do 2 g maščobe. Metoda ne velja za temno obarvane maščobe in olja, npr. surovo palmovo olje. Encim ni specifičen za holesterol, ampak oksidira tudi druge 3-hidroksisterole. Metoda ni bila preskušena za proizvode, ojačane z večjimi koncentracijami sterolov.
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 11702:2010
01-marec-2010
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD(QFLPVNRGRORþHYDQMHFHORWQLKVWHURORY
,62
Animal and vegetable fats and oils - Enzymatic determination of total sterols content
(ISO 11702:2009)
Tierische und pflanzliche Fette und Öle - Enzymatische Bestimmung des Gesamtsterin-
Gehaltes (ISO 11702:2009)
Corps gras d'origines animale et végétale - Détermination enzymatique de la teneur en
stérols totaux (ISO 11702:2009)
Ta slovenski standard je istoveten z: EN ISO 11702:2009
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
SIST EN ISO 11702:2010 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN ISO 11702:2010
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SIST EN ISO 11702:2010
EUROPEAN STANDARD
EN ISO 11702
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2009
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Enzymatic determination of
total sterols content (ISO 11702:2009)
Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Enzymatische
enzymatique de la teneur en stérols totaux (ISO Bestimmung des Gesamtsterin-Gehaltes (ISO 11702:2009)
11702:2009)
This European Standard was approved by CEN on 18 November 2009.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2009 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11702:2009: E
worldwide for CEN national Members.
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SIST EN ISO 11702:2010
EN ISO 11702:2009 (E)
Contents Page
Foreword .3
2
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SIST EN ISO 11702:2010
EN ISO 11702:2009 (E)
Foreword
This document (EN ISO 11702:2009) has been prepared by Technical Committee ISO/TC 34 "Food products"
in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their
by-products - Methods of sampling and analysis”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by June 2010, and conflicting national standards shall be withdrawn at
the latest by June 2010.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 11702:2009 has been approved by CEN as a EN ISO 11702:2009 without any modification.
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SIST EN ISO 11702:2010
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SIST EN ISO 11702:2010
INTERNATIONAL ISO
STANDARD 11702
First edition
2009-12-15
Animal and vegetable fats and oils —
Enzymatic determination of total sterols
content
Corps gras d'origines animale et végétale — Détermination
enzymatique de la teneur en stérols totaux
Refer
...
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