EN ISO 15303:2008
(Main)Animal and vegetable fats and oils - Detection and identification of a volatile organic contaminant by GC/MS (ISO 15303:2001)
Animal and vegetable fats and oils - Detection and identification of a volatile organic contaminant by GC/MS (ISO 15303:2001)
This International Standard specifies a method for the detection and identification of a volatile organic contaminant
in edible oils.
It is applicable to the identification of volatile industrial chemicals in both crude and refined edible oils that are
suspected of being contaminated. It also enables determination of the concentration of the contaminant.
This International Standard is not applicable to the determination of the concentration of chemicals that may react
with the edible oil or with one of its natural components. In these cases, the presence of the contaminant may
sometimes be established on a qualitative basis. Also, this International Standard is not applicable to non-volatile
chemicals.
This method has been shown to be applicable for the identification of the following compound classes:
_ saturated halogenated hydrocarbons;
_ unsaturated halogenated hydrocarbons;
_ esters; aldehydes; alcohols; amines; ketones; ethers;
_ cyclic and aromatic compounds;
_ nitrogen compounds;
_ acrylates; etc.
The method has been evaluated for concentrations in the range of 1 mg/kg to 10 mg/kg.
Tierische und pflanzliche Fette und Öle - Nachweis und Identifizierung einer flüchtigen organischen Verunreinigung mittels GC/MS (ISO 15303:2001)
Diese Internationale Norm legt ein Verfahren zum Nachweis und zur Identifizierung einer flüchtigen orga-nischen Verunreinigung in Speiseölen fest.
Sie ist anwendbar auf die Identifizierung von flüchtigen Industriechemikalien sowohl in rohen als auch in raffinierten Speiseölen, bei denen ein Verdacht auf Verunreinigung besteht. Sie ermöglicht auch die Bestim-mung der Konzentration der Verunreinigung.
Diese Internationale Norm ist nicht auf die Bestimmung der Konzentration von Chemikalien anwendbar, die mit Speiseöl oder einem der natürlichen Bestandteile davon reagieren können. In diesen Fällen kann das Vorliegen der Verunreinigung manchmal qualitativ festgestellt werden. Diese Internationale Norm ist auch nicht auf nichtflüchtige Chemikalien anwendbar.
Es wurde nachgewiesen, dass das vorliegende Verfahren auf die Identifizierung folgender Verbindungs-klassen anwendbar ist:
gesättigte Halogenkohlenwasserstoffe;
ungesättigte Halogenkohlenwasserstoffe;
Ester, Aldehyde, Alkohole, Amine, Ketone, Ether;
cyclische und aromatische Verbindungen;
Stickstoffverbindungen;
Acrylate usw.
Das Verfahren wurde für einen Konzentrationsbereich von 1 mg/kg bis 10 mg/kg bewertet.
Corps gras d'origines animale et végétale - Détection et identification d'un contaminant organique volatil par CPG/SM (ISO 15303:2001)
La présente Norme internationale spécifie une méthode pour la détection et l'identification d'un contaminant
organique volatil dans les huiles comestibles.
Elle est applicable à l'identification des produits chimiques industriels volatils dans les huiles comestibles tant
brutes que raffinées suspectées d'être contaminées. Elle permet également la détermination de la concentration du
contaminant.
La présente Norme internationale n'est pas applicable à la détermination de la concentration des produits
chimiques qui peuvent réagir avec l'huile comestible ou avec l'un de ses constituants naturels. Dans ce cas, il est
quelquefois possible de déterminer la présence du contaminant par une analyse qualitative. La présente Norme
internationale n'est pas non plus applicable aux produits chimiques non volatils.
La présente méthode a été reconnue comme pouvant s'appliquer à l'identification des composés appartenant aux
classes suivantes:
_ hydrocarbures saturés halogénés;
_ hydrocarbures non saturés halogénés;
_ esters; aldéhydes; alcools; amines; cétones; éthers;
_ composés cycliques et aromatiques;
_ composés azotés;
_ acrylates; etc.
La méthode a été testée pour des concentrations comprises entre 1 mg/kg et 10 mg/kg.
Rastlinske in živalske maščobe in olja - Odkrivanje in ugotavljanje hlapnih organskih nečistoč z GC/MS (ISO 15303:2001)
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
01-april-2009
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD2GNULYDQMHLQXJRWDYOMDQMHKODSQLK
RUJDQVNLKQHþLVWRþ]*&06 ,62
Animal and vegetable fats and oils - Detection and identification of a volatile organic
contaminant by GC/MS (ISO 15303:2001)
Tierische und pflanzliche Fette und Öle - Nachweis und Identifizierung einer flüchtigen
organischen Verunreinigung mittels GC/MS (ISO 15303:2001)
Corps gras d'origines animale et végétale - Détection et identification d'un contaminant
organique volatil par CPG/SM (ISO 15303:2001)
Ta slovenski standard je istoveten z: EN ISO 15303:2008
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 15303
NORME EUROPÉENNE
EUROPÄISCHE NORM
November 2008
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Detection and identification
of a volatile organic contaminant by GC/MS (ISO 15303:2001)
Corps gras d'origines animale et végétale - Détection et Tierische und pflanzliche Fette und Öle - Nachweis und
identification d'un contaminant organique volatil par Identifizierung einer flüchtigen organischen Verunreinigung
CPG/SM (ISO 15303:2001) mittels GC/MS (ISO 15303:2001)
This European Standard was approved by CEN on 23 October 2008.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15303:2008: E
worldwide for CEN national Members.
Contents Page
Foreword.3
Foreword
The text of ISO 15303:2001 has been prepared by Technical Committee ISO/TC 34 “Agricultural food
products” of the International Organization for Standardization (ISO) and has been taken over as EN ISO
15303:2008 by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-
products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by May 2009, and conflicting national standards shall be withdrawn at the
latest by May 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 15303:2001 has been approved by CEN as a EN ISO 15303:2008 without any modification.
INTERNATIONAL ISO
STANDARD 15303
First edition
2001-09-15
Animal and vegetable fats and oils —
Detection and identification of a volatile
organic contaminant by GC/MS
Corps gras d'origines animale et végétale — Détection et identification d'un
contaminant organique volatil par CPG/SM
Reference number
ISO 15303:2001(E)
©
ISO 2001
ISO 15303:2001(E)
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ii © ISO 2001 – All rights reserved
ISO 15303:2001(E)
Contents Page
Foreword.iv
1 Scope .1
2 Normative reference .1
3 Principle.1
4 Reagents.2
5 Apparatus .2
6 Procedure .3
6.1 Preparation of standard and blank .3
6.2 Preparation of internal standards .3
6.3 Preparation of analytical sample.3
6.4 Determination by GC/MS analysis .3
6.5 Identification.4
7 Calculation and expression of results.4
7.1 Calculation.4
7.2 Expression of results .5
8 Precision.5
8.1 Interlaboratory tests .5
8.2 Repeatability.5
8.3 Reproducibility.5
Annex A (informative) Results of interlaboratory tests.6
Bibliography.10
ISO 15303:2001(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 15303 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 11, Animal and vegetable fats and oils.
Annex A of this International Standard is for information only.
iv © ISO 2001 – All rights reserved
INTERNATIONAL STANDARD ISO 15303:2001(E)
Animal and vegetable fats and oils — Detection and identification
of a volatile organic contaminant by GC/MS
1 Scope
This International Standard specifies a method for the detection and identification of a volatile organic contaminant
in edible oils.
It is applicable to the identification of volatile industrial chemicals in both crude and refined edible oils that are
suspected of being contaminated. It also enables determination of the concentration of the contaminant.
This International Standard is not applicable to the determination of the concentration of chemicals that may react
with the edible oil or with one of its natural components. In these cases, the presence of the contaminant may
sometimes be established on a qualitative basis. Also, this International Standard is not applicable to non-volatile
chemicals.
This method has been shown to be applicable for the identification of the following compound classes:
� saturated halogenated hydrocarbons;
� unsaturated halogenated hydrocarbons;
� esters; aldehydes; alcohols; amines; ketones; ethers;
� cyclic and aromatic compounds;
� nitrogen compounds;
� acrylates; etc.
The method has been evaluated for concentrations in the range of 1 mg/kg to 10 mg/kg.
2 Normative reference
The following normative document contains provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent edition of the normative document indicated below. For
undated references, the latest edition of the normative document referred to applies. Members of ISO and IEC
maintain registers of currently valid International Standards.
ISO 661:1989, Animal and vegetable fats and oils — Preparation of test sample
3Principle
A deuterated reference compound with a GC retention time close to that of the suspected contaminant is added to
the oil at a concentration close to that of the suspected contaminant. A sample of the oil is then introduced into a
ISO 15303:2001(E)
thermal cold-trapping inlet of a GC/MS apparatus. Volatile components evaporate from the oil and are held in the
cold trap. The trap is then flash-heated to 160 °C and the chemicals released from the trap are swept into the
GC/MS for analysis.
4 Reagents
Use only reagents of recognized analytical grade, and distilled or demineralized water or water of equivalent purity.
4.1 Standard reference compound, corresponding to suspected contaminating compound (99 % pure).
4.2 Methanol, Analar grade.
4.3 Fully deuterated internal standards of benzene, ethyl benzene or naphthalene (99 % pure).
4.4 Helium, chemically pure grade.
4.5 Refined, bleached, deodorized (RBD) groundnut oil, o
...
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