EN ISO 12228-1:2014

Determination of individual and total sterols contents - Gas chromatographic method - Part 1: Animal and vegetable fats and oils (ISO 12228-1:2014)

ISO 12228-1:2014 specifies a procedure for the gas chromatographic determination of the content and composition of sterols in animal and vegetable fats and oils. However, the determination of the contents and composition of sterols in olive and olive pomace oils is to be carried out using ISO 12228-2.

Bestimmung der individuellen und der Gesamtsterine - Gaschromatographisches Verfahren - Teil 1: Tierische und pflanzliche Fette und Öle (ISO 12228-1:2014)

Dieser Teil von ISO 12228 legt ein Verfahren zur gaschromatographischen Bestimmung des Gehalts an Sterinen und deren Zusammensetzung in tierischen und pflanzlichen Fetten und Ölen fest. Jedoch muss für die Bestimmung des Gehalts an Sterinen und deren Zusammensetzung in Oliven- und Oliventresterölen Teil 2 dieser Norm angewendet werden.

Détermination de la teneur en stérols individuels et totaux - Méthode par chromatographie en phase gazeuse - Partie 1: Corps gras d'origines animale et végétale (ISO 12228-1:2014)

L'ISO 12228-1:2014 spécifie un mode opératoire de détermination, par chromatographie en phase gazeuse, de la teneur et de la composition des stérols contenus dans les corps gras d'origines animale et végétale. Toutefois, la détermination de la teneur et de la composition des stérols contenus dans les huiles d'olive et de grignons d'olive doit être réalisée en utilisant l'ISO 12228-2.

Določevanje posameznih in celotnih sterolov - Plinska kromatografska metoda - 1. del: Rastlinske in živalske maščobe in olja (ISO 12228-1:2014)

Standard EN ISO 12228-1 določa postopek za plinsko kromatografsko določevanje vsebnosti in sestave sterolov v živalskih in zelenjavnih maščobah ter oljih. Vendar pa naj bi se določevanje vsebnosti in sestave sterolov v oljčnem olju in oljčnem olju, pridobljenem iz oljčnih tropin, izvajalo z uporabo standarda ISO 12228-2.

General Information

• Status: Published
• Publication Date: 15-Jul-2014
• Withdrawal Date: 30-Jan-2015
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 16-Jul-2014
• Completion Date: 16-Jul-2014
• Ref Project: SIST EN ISO 12228-1:2014 - Determination of individual and total sterols contents - Gas chromatographic method - Part 1: Animal and vegetable fats and oils (ISO 12228-1:2014)

Relations

• Replaces: EN ISO 12228:1999 - Animal and vegetable fats and oils - Determination of individual and total sterols contents - Gas chromatographic method (ISO 12228:1999)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2014
1DGRPHãþD
SIST EN ISO 12228:2000
'RORþHYDQMHSRVDPH]QLKLQFHORWQLKVWHURORY3OLQVNDNURPDWRJUDIVNDPHWRGD
GHO5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD,62
Determination of individual and total sterols contents - Gas chromatographic method -
Part 1: Animal and vegetable fats and oils (ISO 12228-1:2014)
Bestimmung der individuellen und der Gesamtsterine - Gaschromatographisches
Verfahren - Teil 1: Tierische und pflanzliche Fette und Öle (ISO 12228-1:2014)
Détermination de la teneur en stérols individuels et totaux - Méthode par
chromatographie en phase gazeuse - Partie 1: Corps gras d'origines animale et végétale
(ISO 12228-1:2014)
Ta slovenski standard je istoveten z: EN ISO 12228-1:2014
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 12228-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2014
ICS 67.200.10 Supersedes EN ISO 12228:1999
English Version
Determination of individual and total sterols contents - Gas
chromatographic method - Part 1: Animal and vegetable fats and
oils (ISO 12228-1:2014)
Détermination de la teneur en stérols individuels et totaux - Bestimmung der individuellen und der Gesamtsterine -
Méthode par chromatographie en phase gazeuse - Partie 1: Gaschromatographisches Verfahren - Teil 1: Tierische und
Corps gras d'origines animale et végétale (ISO 12228- pflanzliche Fette und Öle (ISO 12228-1:2014)
1:2014)
This European Standard was approved by CEN on 14 June 2014.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2014 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 12228-1:2014 E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
This document (EN ISO 12228-1:2014) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and
oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2015, and conflicting national standards shall be withdrawn at
the latest by January 2015.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 12228:1999.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 12228-1:2014 has been approved by CEN as EN ISO 12228-1:2014 without any modification.

INTERNATIONAL ISO
STANDARD 12228-1
First edition
2014-07-15
Determination of individual
and total sterols contents — Gas
chromatographic method —
Part 1:
Animal and vegetable fats and oils
Détermination de la teneur en stérols individuels et totaux —
Méthode par chromatographie en phase gazeuse —
Partie 1: Corps gras d’origines animale et végétale
Reference number
ISO 12228-1:2014(E)
©
ISO 2014
ISO 12228-1:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
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Tel. + 41 22 749 01 11
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Published in Switzerland
ii © ISO 2014 – All rights reserved

ISO 12228-1:2014(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 3
7 Sample . 3
7.1 Sampling . 3
7.2 Preparation of the test sample . 3
8 Procedure. 4
8.1 Preparation of the aluminium oxide column . 4
8.2 Test portion . 4
8.3 Extraction of the unsaponifiable matter . 4
8.4 Thin-layer chromatography . 4
8.5 Isolation of the sterols . 4
8.6 Preparation of sterol trimethylsilyl ethers . 5
8.7 Gas chromatography . 5
9 Expression of results . 5
9.1 Identification of sterols . 5
9.2 Composition of sterols . 5
9.3 Determination of the total sterol content . 6
10 Precision . 7
10.1 Interlaboratory test. 7
10.2 Repeatability limit, r . 7
10.3 Reproducibility limit, R . 7
11 Test report . 7
Annex A (informative) Figures . 8
Annex B (informative) Interlaboratory trial .15
Bibliography .23
ISO 12228-1:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any
patent rights identified during the development of the document will be in the Introduction and/or on
the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
This first edition of ISO 12228-1, together with ISO 12228-2, cancels
...