ISO 6561-2:2005
(Main)Fruits, vegetables and derived products — Determination of cadmium content — Part 2: Method using flame atomic absorption spectrometry
Fruits, vegetables and derived products — Determination of cadmium content — Part 2: Method using flame atomic absorption spectrometry
ISO 6561-2:2005 specifies an atomic absorption spectrometric method for the determination of the cadmium content of fruits, vegetables and derived products.
Fruits, légumes et produits dérivés — Détermination de la teneur en cadmium — Partie 2: Méthode par spectrométrie d'absorption atomique avec flamme
Sadje, zelenjava in sadni ter zelenjavni proizvodi - Določevanje kadmija - 2. del: Metoda s plamensko atomsko absorpcijo
Ta del ISO 6561 določa spektrometrično metodo z atomsko absorpcijo za določevanje kadmija v sadju, zelenjavi in sadnih ter zelenjavnih proizvodih.
OPOMBA: Metoda določevanja kadmija v sadju, zelenjavi in sadnih ter zelenjavnih proizvodih temelji na Uradnih analitskih metodah AOAC [1].
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INTERNATIONAL ISO
STANDARD 6561-2
First edition
2005-02-01
Fruits, vegetables and derived
products — Determination of cadmium
content —
Part 2:
Method using flame atomic absorption
spectrometry
Fruits, légumes et produits dérivés — Détermination de la teneur en
cadmium —
Partie 2: Méthode par spectrométrie d'absorption atomique avec
flamme
Reference number
ISO 6561-2:2005(E)
©
ISO 2005
---------------------- Page: 1 ----------------------
ISO 6561-2:2005(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.
© ISO 2005
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2005 – All rights reserved
---------------------- Page: 2 ----------------------
ISO 6561-2:2005(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 6561-2 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 3, Fruit and
vegetable products.
This first edition of ISO 6561-2, together with ISO 6561-1:2004, cancels and replaces ISO 6561:1983, which
has been technically revised.
ISO 6561 consists of the following parts, under the general title Fruits, vegetables and derived products —
Determination of cadmium content:
Part 1: Method using graphite furnace atomic absorption spectrometry
Part 2: Method using flame atomic absorption spectrometry
© ISO 2005 – All rights reserved iii
---------------------- Page: 3 ----------------------
INTERNATIONAL STANDARD ISO 6561-2:2005(E)
Fruits, vegetables and derived products — Determination of
cadmium content —
Part 2:
Method using flame atomic absorption spectrometry
1 Scope
This part of ISO 6561 specifies an atomic absorption spectrometric method for the determination of the
cadmium content of fruits, vegetables and derived products.
NOTE The method of cadmium determination in fruit, vegetables and derived products is based on AOAC Official
[1]
Methods of Analysis .
2 Principle
This method is based on the decomposition of organic matter with HNO , H SO and H O , extraction of
3 2 4 2 2
cadmium by dithizone-CHCl at pH 9, and determination of cadmium by flame atomic absorption spectrometry.
3
3 Reagents
Use only reagents of recognized analytical grade, unless otherwise specified, and water which has been
distilled twice in borosilicate glass apparatus, or water of at least equivalent purity.
3.1 Nitric acid, concentrated (ρ = 1,38 g/ml).
20
3.2 Sulfuric acid, concentrated (ρ = 1,84 g/ml).
20
3.3 Hydrochloric acid, dilute, 0,2 mol/l.
Place 16,5 ml of concentrated hydrochloric acid (ρ = 1,19 g/ml) into a 1 000 ml one-mark volumetric flask
20
and dilute to the mark with water. Mix.
3.4 Hydrogen peroxide, concentrated (50 %).
3.5 Citric acid monohydrate.
3.6 Sodium hydroxide, 0,05 mol/l solution.
3.7 Thymol blue indicator.
Triturate 0,1 g of thymol blue indicator in an agate mortar with 4,3 ml of sodium hydroxide solution (3.6). Dilute
to 200 ml in a flask with water.
3.8 Ammonia solution, 28 % to 30 % solution.
© ISO 2005 – All rights reserved 1
---------------------- Page: 4 ----------------------
ISO 6561-2:2005(E)
3.9 Chloroform.
3.10 Dithizone, 1,0 mg/ml solution.
Place 0,2 g of dithizone in a 200 ml volumetric flask and add chloroform to the mark.
3.11 Dithizone, 0,2 mg/ml solution.
Dilute dithizone solution (3.10) 1 + 4 with chloroform (3.9). Prepare fresh daily.
3.12 Cadmium standard solution, corresponding to a cadmium concentration of 1,0 mg/ml.
4 Apparatus
Before use, wash all glassware with 8 mol/l nitric acid, followed by a thorough rinse with water.
Cover beakers with watch glasses during all operations.
Usual laboratory apparatus and, in particular, the following.
4.1 Mechanical grinder, the inside and the blades of which are coated with polytetrafluoroethylene (PTFE).
4.2 Round-bottom flasks, of 1 500 ml capacity.
4.3 Beaker, of 400 ml capacity.
4.4 One-mark volumetric flasks, of capacities 50 ml, 100 ml and 1 000 ml.
4.5 Pipettes, of appropriate capacity.
4.6 Separators, 125 ml and 250 ml capacities.
4.7 Atomic absorption spectrometer, provided with an air/acetylene burner (10 cm), suitable for
measurements at a wavelength of 228,8 nm.
4.8 Burner or heating mantle.
4.9 Hot plate.
4.10 Analytical balance.
5 Sampling
A representative sample should have been sent to the laboratory. It should not have been damaged or
changed during transport or storage.
Sampling is not part of the method specified in this part of ISO 6561. If there is no specific International
Standard dealing with the product concerned, it is recommended that the parties concerned come to an
agreement on the subject.
2 © ISO 2005 – All rights reserved
---------------------- Page: 5 ----------------------
ISO 6561-2:2005(E)
6 Procedure
6.1 Preparation of test sample
Mix well the laboratory sample, if necessary, first remove any seeds, stalks and hard seed-cavity walls, and
then grind in the mechanical grinder (4.1).
Frozen or deep-frozen products shall be previously thawed in a closed vessel, and the liquid formed during
this process shall be added to the product before mixing.
6.2 Test portion
Weigh, to the nearest 0,01 g, 50 g of the test sample (6.1) into a 1 500 ml round-bottom flask (4.2).
6.3 Decomposition
If the test portion contains ethanol, first remove the ethanol by evaporation.
Add several boiling chips or beads and carefully add
...
SLOVENSKI STANDARD
SIST ISO 6561-2:2011
01-junij-2011
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SIST ISO 6561:1995
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Fruits, vegetables and derived products -- Determination of cadmium content -- Part 2:
Method using flame atomic absorption spectrometry
Fruits, légumes et produits dérivés -- Détermination de la teneur en cadmium -- Partie 2:
Méthode par spectrométrie d'absorption atomique avec flamme
Ta slovenski standard je istoveten z: ISO 6561-2:2005
ICS:
67.080.01 Sadje, zelenjava in njuni Fruits, vegetables and
proizvodi na splošno derived products in general
SIST ISO 6561-2:2011 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
SIST ISO 6561-2:2011
---------------------- Page: 2 ----------------------
SIST ISO 6561-2:2011
INTERNATIONAL ISO
STANDARD 6561-2
First edition
2005-02-01
Fruits, vegetables and derived
products — Determination of cadmium
content —
Part 2:
Method using flame atomic absorption
spectrometry
Fruits, légumes et produits dérivés — Détermination de la teneur en
cadmium —
Partie 2: Méthode par spectrométrie d'absorption atomique avec
flamme
Reference number
ISO 6561-2:2005(E)
©
ISO 2005
---------------------- Page: 3 ----------------------
SIST ISO 6561-2:2011
ISO 6561-2:2005(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.
© ISO 2005
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2005 – All rights reserved
---------------------- Page: 4 ----------------------
SIST ISO 6561-2:2011
ISO 6561-2:2005(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 6561-2 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 3, Fruit and
vegetable products.
This first edition of ISO 6561-2, together with ISO 6561-1:2004, cancels and replaces ISO 6561:1983, which
has been technically revised.
ISO 6561 consists of the following parts, under the general title Fruits, vegetables and derived products —
Determination of cadmium content:
Part 1: Method using graphite furnace atomic absorption spectrometry
Part 2: Method using flame atomic absorption spectrometry
© ISO 2005 – All rights reserved iii
---------------------- Page: 5 ----------------------
SIST ISO 6561-2:2011
---------------------- Page: 6 ----------------------
SIST ISO 6561-2:2011
INTERNATIONAL STANDARD ISO 6561-2:2005(E)
Fruits, vegetables and derived products — Determination of
cadmium content —
Part 2:
Method using flame atomic absorption spectrometry
1 Scope
This part of ISO 6561 specifies an atomic absorption spectrometric method for the determination of the
cadmium content of fruits, vegetables and derived products.
NOTE The method of cadmium determination in fruit, vegetables and derived products is based on AOAC Official
[1]
Methods of Analysis .
2 Principle
This method is based on the decomposition of organic matter with HNO , H SO and H O , extraction of
3 2 4 2 2
cadmium by dithizone-CHCl at pH 9, and determination of cadmium by flame atomic absorption spectrometry.
3
3 Reagents
Use only reagents of recognized analytical grade, unless otherwise specified, and water which has been
distilled twice in borosilicate glass apparatus, or water of at least equivalent purity.
3.1 Nitric acid, concentrated (ρ = 1,38 g/ml).
20
3.2 Sulfuric acid, concentrated (ρ = 1,84 g/ml).
20
3.3 Hydrochloric acid, dilute, 0,2 mol/l.
Place 16,5 ml of concentrated hydrochloric acid (ρ = 1,19 g/ml) into a 1 000 ml one-mark volumetric flask
20
and dilute to the mark with water. Mix.
3.4 Hydrogen peroxide, concentrated (50 %).
3.5 Citric acid monohydrate.
3.6 Sodium hydroxide, 0,05 mol/l solution.
3.7 Thymol blue indicator.
Triturate 0,1 g of thymol blue indicator in an agate mortar with 4,3 ml of sodium hydroxide solution (3.6). Dilute
to 200 ml in a flask with water.
3.8 Ammonia solution, 28 % to 30 % solution.
© ISO 2005 – All rights reserved 1
---------------------- Page: 7 ----------------------
SIST ISO 6561-2:2011
ISO 6561-2:2005(E)
3.9 Chloroform.
3.10 Dithizone, 1,0 mg/ml solution.
Place 0,2 g of dithizone in a 200 ml volumetric flask and add chloroform to the mark.
3.11 Dithizone, 0,2 mg/ml solution.
Dilute dithizone solution (3.10) 1 + 4 with chloroform (3.9). Prepare fresh daily.
3.12 Cadmium standard solution, corresponding to a cadmium concentration of 1,0 mg/ml.
4 Apparatus
Before use, wash all glassware with 8 mol/l nitric acid, followed by a thorough rinse with water.
Cover beakers with watch glasses during all operations.
Usual laboratory apparatus and, in particular, the following.
4.1 Mechanical grinder, the inside and the blades of which are coated with polytetrafluoroethylene (PTFE).
4.2 Round-bottom flasks, of 1 500 ml capacity.
4.3 Beaker, of 400 ml capacity.
4.4 One-mark volumetric flasks, of capacities 50 ml, 100 ml and 1 000 ml.
4.5 Pipettes, of appropriate capacity.
4.6 Separators, 125 ml and 250 ml capacities.
4.7 Atomic absorption spectrometer, provided with an air/acetylene burner (10 cm), suitable for
measurements at a wavelength of 228,8 nm.
4.8 Burner or heating mantle.
4.9 Hot plate.
4.10 Analytical balance.
5 Sampling
A representative sample should have been sent to the laboratory. It should not have been damaged or
changed during transport or storage.
Sampling is not part of the method specified in this part of ISO 6561. If there is no specific International
Standard dealing with the product concerned, it is recommended that the parties concerned come to an
agreement on the subject.
2 © ISO 2005 – All rights reserved
---------------------- Page: 8 ----------------------
SIST ISO 6561-2:2011
ISO 6561-2:2005(E)
6 Procedure
6.1 Prepa
...
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