ISO 13365-2:2020

Leather — Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography — Part 2: Artificial perspiration extraction method

This document specifies a test method by artificial perspiration solution aqueous extraction for the determination of the aqueous extractable content of the following preservative agents in leather by liquid chromatography: — 2-(thiocyanomethylthio)-benzothiazole (TCMTB); — 4-chloro-3-methylphenol (PCMC); — 2-phenylphenol (OPP); — 2-octylisothiazol-3(2H)-one (OIT); This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.

Cuir — Dosage chimique des agents de conservation (TCMTB, PCMC, OPP, OIT) dans le cuir par chromatographie en phase liquide — Partie 2: Extraction à la sueur artificielle

Le présent document spécifie une méthode d'essai pour la détermination de la teneur extractible par l'eau des agents de conservation dans le cuir par chromatographie en phase liquide, après extraction par une solution aqueuse de sueur artificielle; elle concerne les agents de conservation suivants: — 2-(thiocyanométhylthio)-benzothiazole (TCMTB); — 4-chloro-3-méthylphénol (PCMC); — 2-phénylphénol (OPP); — 2-octylisothiazol-3(2H)-one (OIT). Cette méthode peut également être utilisée pour déterminer les produits de décomposition de ces agents de conservation, qui protègent le cuir des attaques microbiologiques.

General Information

• Status: Published
• Publication Date: 02-Aug-2020
• ICS: 59.140.30 - Leather and furs
• Technical Committee: IULTCS - International Union of Leather Technologists and Chemists Societies
• Drafting Committee: IULTCS - International Union of Leather Technologists and Chemists Societies
• Current Stage: 6060 - International Standard published
• Start Date: 31-Jul-2020
• Due Date: 07-Mar-2020
• Completion Date: 03-Aug-2020

Relations

• Consolidates: ISO/IEC 19464:2014 - Information technology — Advanced Message Queuing Protocol (AMQP) v1.0 specification
• Effective Date: 06-Jun-2022
• Revises: ISO 13365:2011 - Leather — Chemical tests — Determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography
• Effective Date: 06-May-2017

Standards Content (Sample)

INTERNATIONAL ISO
STANDARD 13365-2
IULTCS
IUC 29-2
First edition
2020-07
Leather — Chemical determination
of the preservative (TCMTB, PCMC,
OPP, OIT) content in leather by liquid
chromatography —
Part 2:
Artificial perspiration extraction
method
Cuir — Dosage chimique des agents de conservation (TCMTB, PCMC,
OPP, OIT) dans le cuir par chromatographie en phase liquide —
Partie 2: Extraction à la sueur artificielle
Reference numbers
ISO 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
©
ISO 2020

---------------------- Page: 1 ----------------------
ISO 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved

---------------------- Page: 2 ----------------------
ISO 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
5.1 General . 2
5.2 Target compounds . 2
5.3 Preparation of calibration solutions . 2
6 Apparatus and materials. 3
7 Testing procedure . 3
7.1 Sampling and sample preparation . 3
7.2 Preparation of acid artificial perspiration solution . 3
7.3 Preparation of analytical solution . 3
7.4 Analytical procedure . 4
8 Calculation . 4
9 Test report . 5
Annex A (informative) Chromatographic conditions . 6
© ISO 2020 – All rights reserved iii

---------------------- Page: 3 ----------------------
ISO 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso .org/
iso/ foreword .html.
This document was prepared by the Chemical Test Commission of the International Union of Leather
Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, the secretariat of
which is held by UNI, in accordance with the agreement on technical cooperation between ISO and CEN
(Vienna Agreement).
IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to
further the advancement of leather science and technology. IULTCS has three commissions, which
are responsible for establishing international methods for the sampling and testing of leather. ISO
recognizes IULTCS as an international standardizing body for the preparation of test methods for
leather.
This first edition of ISO 13365-2, together with ISO 13365-1, cancels and replaces ISO 13365:2011,
which has been technically revised and split into two parts. The main changes in ISO 13365-2 from
ISO 13365:2011 are as follows:
— the title has been changed to indicate the method of extraction;
— the use of HPLC with mass spectrometric (MS) detection has been included;
— Clause 5 (former Clause 4) has been technically modified. In addition, the calibration information
(previously in 6.4) is now included in Clause 5;
— the chromatographic conditions (previously in 6.3) are now included in a new Annex A. Annex A
also includes additional conditions for use with MS detection.
A list of all parts in the ISO 13365 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved

---------------------- Page: 4 ----------------------
ISO 13365-2:2020(E)
INTERNATIONAL STANDARD
IULTCS/IUC 29-2:2020(E)
Leather — Chemical determination of the preservative
(TCMTB, PCMC, OPP, OIT) content in leather by liquid
chromatography —
Part 2:
Artificial perspiration extraction method
1 Scope
This document specifies a test method by artificial perspiration solution aqueous extraction for the
determination of the aqueous extractable content of the following preservative agents in leather by
liquid chromatography:
— 2-(thiocyanomethylthio)-benzothiazole (TCMTB);
— 4-chloro-3-methylphenol (PCMC);
— 2-phenylphenol (OPP);
— 2-octylisothiazol-3(2H)-one (OIT);
This method can also be used to determine breakdown products of these preservative agents, which
protect leather from microbiological attack.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated refer
...

NORME ISO
INTERNATIONALE 13365-2
IULTCS
IUC 29-2
Première édition
2020-07
Cuir — Dosage chimique des agents
de conservation (TCMTB, PCMC, OPP,
OIT) dans le cuir par chromatographie
en phase liquide —
Partie 2:
Extraction à la sueur artificielle
Leather — Chemical determination of the preservative (TCMTB,
PCMC, OPP, OIT) content in leather by liquid chromatography —
Part 2: Artificial perspiration extraction method
Numéros de référence
ISO 13365-2:2020(F)
IULTCS/IUC 29-2:2020(F)
©
ISO 2020

---------------------- Page: 1 ----------------------
ISO 13365-2:2020(F)
IULTCS/IUC 29-2:2020(F)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2020
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
y compris la photocopie, ou la diffusion sur l’internet ou sur un intranet, sans autorisation écrite préalable. Une autorisation peut
être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: [phone]
E-mail: [e-mail]
Web: www.iso.org
Publié en Suisse
ii © ISO 2020 – Tous droits réservés

---------------------- Page: 2 ----------------------
ISO 13365-2:2020(F)
IULTCS/IUC 29-2:2020(F)
Sommaire Page
Avant-propos .iv
1 Domaine d'application . 1
2 Références normatives . 1
3 Termes et définitions . 1
4 Principe . 2
5 Réactifs . 2
5.1 Généralités . 2
5.2 Composés cibles . 2
5.3 Préparation des solutions d'étalonnage. 3
6 Appareillage et matériaux . 3
7 Mode opératoire d'essai . 3
7.1 Échantillonnage et préparation des échantillons . 3
7.2 Préparation de la solution de sueur artificielle acide . 3
7.3 Préparation de la solution pour analyse . 4
7.4 Mode opératoire d'analyse . 4
8 Calcul . 4
9 Rapport d'essai . 5
Annexe A (informative) Conditions chromatographiques . 6
© ISO 2020 – Tous droits réservés iii

---------------------- Page: 3 ----------------------
ISO 13365-2:2020(F)
IULTCS/IUC 29-2:2020(F)
Avant-propos
L'ISO (Organisation internationale de normalisation) est une fédération mondiale d'organismes
nationaux de normalisation (comités membres de l'ISO). L'élaboration des Normes internationales est
en général confiée aux comités techniques de l'ISO. Chaque comité membre intéressé par une étude
a le droit de faire partie du comité technique créé à cet effet. Les organisations internationales,
gouvernementales et non gouvernementales, en liaison avec l'ISO participent également aux travaux.
L'ISO collabore étroitement avec la Commission électrotechnique internationale (IEC) en ce qui
concerne la normalisation électrotechnique.
Les procédures utilisées pour élaborer le présent document et celles destinées à sa mise à jour sont
décrites dans les Directives ISO/IEC, Partie 1. Il convient, en particulier de prendre note des différents
critères d'approbation requis pour les différents types de documents ISO. Le présent document a été
rédigé conformément aux règles de rédaction données dans les Directives ISO/IEC, Partie 2 (voir www
.iso .org/ directives).
L'attention est attirée sur le fait que certains des éléments du présent document peuvent faire l'objet de
droits de propriété intellectuelle ou de droits analogues. L'ISO ne saurait être tenue pour responsable
de ne pas avoir identifié de tels droits de propriété et averti de leur existence. Les détails concernant
les références aux droits de propriété intellectuelle ou autres droits analogues identifiés lors de
l'élaboration du document sont indiqués dans l'Introduction et/ou dans la liste des déclarations de
brevets reçues par l'ISO (voir www .iso .org/ brevets).
Les appellations commerciales éventuellement mentionnées dans le présent document sont données
pour information, par souci de commodité, à l’intention des utilisateurs et ne sauraient constituer un
engagement.
Pour une explication de la nature volontaire des normes, la signification des termes et expressions
spécifiques de l'ISO liés à l'évaluation de la conformité, ou pour toute information au sujet de l'adhésion
de l'ISO aux principes de l’Organisation mondiale du commerce (OMC) concernant les obstacles
techniques au commerce (OTC), voir www .iso .org/ iso/ fr/ avant -propos .html.
Le présent document a été élaboré par le comité technique CEN/TC 289, Cuir, du Comité européen de
normalisation (CEN), dont le secrétariat est tenu par UNI, en collaboration avec la Commission des essais
chimiques de l'Union internationale des sociétés de techniciens et chimistes du cuir (commission IUC,
IULTCS), conformément à l'Accord de coopération technique entre l'ISO et le CEN (Accord de Vienne).
L'IULTCS est une organisation mondiale de sociétés professionnelles des industries du cuir fondée
en 1897 ayant pour mission de favoriser l'avancement des sciences et technologies du cuir. L'IULTCS
a trois commissions, qui sont responsables de l'établissement des méthodes internationales
d'échantillonnage et d'essai des cuirs. L'ISO reconnaît l'IULTCS en tant qu'organisme international à
activités normatives pour l'élaboration de méthodes d'essai relatives au cuir.
Cette première édition de l’ISO 13365-2, conjointement à l’ISO 13365-1, annule et remplace
l’ISO 13365:2011, qui a fait l’objet d’une révision technique et a été divisée en deux parties. Les
principales modifications de l’ISO 13365-2 par rapport à l’ISO 13365:2011 sont les suivantes:
— le titre a été modifié pour indiquer la méthode d’extraction;
— la méthode par chromatographie liquide à haute performance (CLHP) avec détection par
spectrométrie de masse (SM) a été ajoutée;
— l’Article 5 (ancien Article 4) a fait l’objet d’une modification technique. En outre, les informations
relatives à l'étalonnage (qui figuraient auparavant en 6.4) sont désormais intégrées à l'Article 5;
— les conditions chromatographiques (qui figuraient auparavant en 6.3) font désormais l’objet d’une
Annexe A, nouvelle. L’Annexe A comporte également des conditions supplémentaires à appliquer
avec la détection SM.
Une liste de toutes les parties de la série ISO 13365 se trouve sur le site web de l'ISO.
iv © ISO 2020 – Tous droits réservés

---------------------- Page: 4 ----------------------
ISO 13365-2:2020(F)
IULTCS/IUC 29-2:2020(F)
Il convient que l'utilisateur adresse tout retour d'information ou toute question concernant le présent
document à l'organisme national de normalisation de son pays. Une liste exhaustive desdits organismes
se trouve à l'adresse www .iso .org/ members .html.
© ISO 2020 – Tous droits réservés v

---------------------- Page: 5 ----------------------
ISO 13365-2:2020(F)
NORME INTERNATIONALE
IULTCS/IUC 29-2:2020(F)
Cuir — Dosage chimique des agents de conservation
(TCMTB, PCMC, OPP, OIT) dans le cuir par chromatographie
en phase liquide —
Partie 2:
Extraction à la sueur artificielle
1 Domaine d'application
Le présent document spécifie une méthode d'essai pour la détermination de la teneur extractible par
l'eau des agents de conservation dans le cuir par chromatographie en phase liquide, après extraction
par une solution aqueuse de sueur artificielle; elle concerne les agents de conservation suivants:
— 2-(thiocyanométhylthio)-benzothiazole (TCMTB);
— 4-chloro-3-méthylphénol (PCMC);
— 2-phénylphénol (OPP);
— 2-octylisothiazol-3(2H)-one (OIT).
Cette méthode peut également être utilisée pour déterminer les produits de décomposition de ces
agents de conservation, qui protègent le cuir des attaques microbiologiques.
2 Références normatives
Les documents suivants sont cités dans le texte de sorte qu’ils constituent, pour tout ou partie de leur
contenu, des exigences du présent document. Pour les références datées, seule l’édition citée s’applique.
Pour les références non datées, la dernière édition du document de référence s'applique (y compris les
éventuels amendements).
ISO 2418, Cuir — Essais chimiques, physiques, mécaniques et de solidité — Emplacem
...

FINAL
INTERNATIONAL ISO/FDIS
DRAFT
STANDARD 13365-2
IULTCS/IUC 29-2
IULTCS
Leather — Chemical determination
Voting begins on:
2020-04-06
of the preservative (TCMTB, PCMC,
Voting terminates on: OPP, OIT) content in leather by liquid
2020-06-01
chromatography —
Part 2:
Artificial perspiration extraction
method
Cuir — Dosage chimiques des agents de conservation (TCMTB, PCMC,
OPP, OIT) dans le cuir par chromatographie en phase liquide —
Partie 2: Extraction à la sueur artificielle
ISO/CEN PARALLEL PROCESSING
RECIPIENTS OF THIS DRAFT ARE INVITED TO
SUBMIT, WITH THEIR COMMENTS, NOTIFICATION
OF ANY RELEVANT PATENT RIGHTS OF WHICH
THEY ARE AWARE AND TO PROVIDE SUPPOR TING
DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
Reference numbers
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/FDIS 13365-2:2020(E)
LOGICAL, COMMERCIAL AND USER PURPOSES,
DRAFT INTERNATIONAL STANDARDS MAY ON
IULTCS/IUC 29-2:2020(E)
OCCASION HAVE TO BE CONSIDERED IN THE
LIGHT OF THEIR POTENTIAL TO BECOME STAN-
DARDS TO WHICH REFERENCE MAY BE MADE IN
©
NATIONAL REGULATIONS. ISO 2020

---------------------- Page: 1 ----------------------
ISO/FDIS 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved

---------------------- Page: 2 ----------------------
ISO/FDIS 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
5.1 General . 2
5.2 Target compounds . 2
5.3 Preparation of calibration solutions . 2
6 Apparatus and materials. 3
7 Testing procedure . 3
7.1 Sampling and sample preparation . 3
7.2 Preparation of acid artificial perspiration solution . 3
7.3 Preparation of analytical solution . 3
7.4 Analytical procedure . 4
8 Calculation . 4
9 Test report . 5
Annex A (informative) Chromatographic conditions . 6
© ISO 2020 – All rights reserved iii

---------------------- Page: 3 ----------------------
ISO/FDIS 13365-2:2020(E)
IULTCS/IUC 29-2:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by the Chemical Test Commission of the International Union of Leather
Technologists and Chemists Societies (IUC Commission, IULTCS) in collaboration with the European
Committee for Standardization (CEN) Technical Committee CEN/TC 289, Leather, the secretariat of
which is held by UNI, in accordance with the agreement on technical cooperation between ISO and CEN
(Vienna Agreement).
IULTCS, originally formed in 1897, is a world-wide organization of professional leather societies to
further the advancement of leather science and technology. IULTCS has three commissions, which
are responsible for establishing international methods for the sampling and testing of leather. ISO
recognizes IULTCS as an international standardizing body for the preparation of test methods for
leather.
This first edition of ISO 13365-2, together with ISO 13365-1, cancels and replaces ISO 13365:2011,
which has been technically revised and split into two parts. The main changes in ISO 13365-2 from
ISO 13365:2011 are as follows:
— the title has been changed to indicate the method of extraction;
— the use of HPLC with mass spectrometric (MS) detection has been included;
— Clause 5 (former Clause 4) has been technically modified. In addition, the calibration information
(previously in 6.4) is now included in Clause 5;
— the chromatographic conditions (previously in 6.3) are now included in a new Annex A. Annex A
also includes additional conditions for use with MS detection.
A list of all parts in the ISO 13365 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved

---------------------- Page: 4 ----------------------
ISO/FDIS 13365-2:2020(E)
FINAL DRAFT INTERNATIONAL STANDARD
IULTCS/IUC 29-2:2020(E)
Leather — Chemical determination of the preservative
(TCMTB, PCMC, OPP, OIT) content in leather by liquid
chromatography —
Part 2:
Artificial perspiration extraction method
1 Scope
This document specifies a test method by artificial perspiration solution aqueous extraction for the
determination of the aqueous extractable content of the following preservative agents in leather by
liquid chromatography:
— 2-(thiocyanomethylthio)-benzothiazole (TCMTB);
— 4-chloro-3-methylphenol (PCMC);
— 2-phenylphenol (OPP);
— 2-octylisothiazol-3(2H)-one (OIT);
This method can also
...