ISO 18071:2016
(Main)Fine ceramics (advanced ceramics, advanced technical ceramics) — Determination of antiviral activity of semiconducting photocatalytic materials under indoor lighting environment — Test method using bacteriophage Q-beta
Fine ceramics (advanced ceramics, advanced technical ceramics) — Determination of antiviral activity of semiconducting photocatalytic materials under indoor lighting environment — Test method using bacteriophage Q-beta
ISO 18071:2016 specifies the determination of the antiviral activity of materials that contain indoor-light-active photocatalytic materials or have indoor-light-active photocatalytic films on the surface by a test method that measures the infectivity titre of bacteriophage Q-beta after illumination with indoor light. NOTE In the test method, the surrogate microbe is bacteriophage Q-beta, intended as a model for influenza viruses. It is intended for use with different kinds of indoor-light-active photocatalytic materials used in construction materials, in flat sheet, board or plate shape that are the basic forms of materials for various applications. It does not include powder, granular or porous indoor-light-active photocatalytic materials. It is applicable to indoor-light-active photocatalytic materials produced for an antiviral applications. Other types of performance of indoor-light-active photocatalytic materials, i.e. antibacterial activity, antifungal activity, decomposition of water contaminants, self-cleaning, antifogging and air purification, are not determined by this method.
Céramiques techniques — Détermination de l'activité antivirale des matériaux photocatalytiques semi-conducteurs dans un environnement d'éclairage intérieur — Méthode d'essai utilisant un bactériophage Q-béta
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DRAFT INTERNATIONAL STANDARD
ISO/DIS 18071
ISO/TC 206 Secretariat: JISC
Voting begins on: Voting terminates on:
2015-11-26 2016-02-26
Fine Ceramics (Advanced Ceramics, Advanced Technical
Ceramics) — Determination of antiviral activity of
semiconducting photocatalytic materials under indoor
lighting environment — Test method using
bacteriophage Q-beta
Titre manque
ICS: 81.060.30
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 18071:2015(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2015
ISO/DIS 18071:2015(E) ISO/DIS 18071
Contents Page
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols (and abbreviated terms) . 2
5 Principle. 3
6 Materials . 3
6.1 Strains and preparation for tests . 3
6.1.1 Strains. 3
6.1.2 Bacteria preparation . 4
6.1.3 Bacteriophage preparation . 4
6.2 Media . 5
6.2.1 General . 5
6.2.2 1/500 Nutrient broth (1/500 NB) . 5
6.2.3 Calcium solution . 5
6.2.4 LB broth with calcium . 5
6.2.5 Agar powder . 5
6.2.6 LB agar . 5
6.2.7 Bottom agar plate (LB agar plate with calcium) . 6
6.2.8 Top agar. 6
6.2.9 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP) . 6
6.2.10 Peptone saline solution . 6
7 Apparatus and equipment . 6
7.1 Test equipment . 6
7.2 Cover film . 7
7.3 Moisture preservation glass . 7
7.4 Glass tube or glass rod . 7
7.5 Paper filter . 7
7.6 Light source . 7
7.7 UV sharp cut-off filter . 8
7.8 Illuminance meter . 8
7.9 Centrifuge . 8
7.10 Sterilized syringe filter unit . 8
8 Test piece . 8
9 Procedure . 9
9.1 General . 9
9.2 Procedure for preparation of bacteria suspension . 9
© ISO 2015, Published in Switzerland
9.3 Procedure of preparation of test bacteriophage solution . 10
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
9.4 Procedure of test for indoor-light-active photocatalytic antiviral activity . 10
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
9.5 Indoor lighting condition . 10
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
9.6 Measurement of titre of bacteriophage . 11
the requester.
ISO copyright office
10 Calculation . 12
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
11 Test report . 13
Tel. +41 22 749 01 11
Bibliography . 15
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2015 – All rights reserved
ISO/DIS 18071
Contents Page
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols (and abbreviated terms) . 2
5 Principle. 3
6 Materials . 3
6.1 Strains and preparation for tests . 3
6.1.1 Strains. 3
6.1.2 Bacteria preparation . 4
6.1.3 Bacteriophage preparation . 4
6.2 Media . 5
6.2.1 General . 5
6.2.2 1/500 Nutrient broth (1/500 NB) . 5
6.2.3 Calcium solution . 5
6.2.4 LB broth with calcium . 5
6.2.5 Agar powder . 5
6.2.6 LB agar . 5
6.2.7 Bottom agar plate (LB agar plate with calcium) . 6
6.2.8 Top agar. 6
6.2.9 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP) . 6
6.2.10 Peptone saline solution . 6
7 Apparatus and equipment . 6
7.1 Test equipment . 6
7.2 Cover film . 7
7.3 Moisture preservation glass . 7
7.4 Glass tube or glass rod . 7
7.5 Paper filter . 7
7.6 Light source . 7
7.7 UV sharp cut-off filter . 8
7.8 Illuminance meter . 8
7.9 Centrifuge . 8
7.10 Sterilized syringe filter unit . 8
8 Test piece . 8
9 Procedure . 9
9.1 General . 9
9.2 Procedure for preparation of bacteria suspension . 9
9.3 Procedure of preparation of test bacteriophage solution . 10
9.4 Procedure o
...
INTERNATIONAL ISO
STANDARD 18071
First edition
2016-07-15
Fine ceramics (advanced ceramics,
advanced technical ceramics) —
Determination of antiviral activity
of semiconducting photocatalytic
materials under indoor lighting
environment — Test method using
bacteriophage Q-beta
Céramiques techniques — Détermination de l’activité antivirale
des matériaux photocatalytiques semi-conducteurs dans un
environnement d’éclairage intérieur — Méthode d’essai utilisant un
bactériophage Q-béta
Reference number
©
ISO 2016
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2016 – All rights reserved
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols . 2
5 Principle . 3
6 Materials . 3
6.1 Strains and preparation for tests . 3
6.1.1 Strains . 3
6.1.2 Bacteria preparation. 4
6.1.3 Bacteriophage preparation . 4
6.2 Media . 5
6.2.1 General. 5
6.2.2 1/500 Nutrient broth (1/500 NB) . 5
6.2.3 Calcium solution . 5
6.2.4 LB broth with calcium . 5
6.2.5 Agar powder . 5
6.2.6 LB agar . 5
6.2.7 Bottom agar plate (LB agar plate with calcium) . 6
6.2.8 Top agar . 6
6.2.9 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP). 6
6.2.10 Peptone saline solution . 6
7 Apparatus and equipment . 6
7.1 Test equipment . 6
7.2 Cover film . 7
7.3 Moisture preservation glass . 7
7.4 Glass tube or glass rod . 7
7.5 Paper filter . 7
7.6 Light source . 7
7.7 UV sharp cut-off filter . 8
7.7.1 Condition A (under 400 nm cut-off condition) . 8
7.7.2 Condition B (under 380 nm cut-off condition) . 8
7.8 Illuminance meter . 8
7.9 Centrifuge . 8
7.10 Sterilized syringe filter unit . 8
8 Test piece . 8
9 Procedure. 8
9.1 General . 8
9.2 Procedure for preparation of bacteria suspension . 9
9.3 Procedure of preparation of test bacteriophage solution . 9
9.4 Procedure of test for indoor-light-active photocatalytic antiviral activity .10
9.5 Indoor lighting condition .10
9.6 Measurement of titre of bacteriophage .11
10 Calculation .12
10.1 General .12
10.2 Test requirement fulfilment validation .12
10.3 Indoor-light-active photocatalyst antiviral activity value calculation .13
10.4 Antiviral activity value calculation without indoor-light-active photocatalyst .13
11 Test report .14
Bibliography .15
iv © ISO 2016 – All rights reserved
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment,
as well as information about ISO’s adherence to the World Trade Organization (WTO) principles in the
Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html.
The committee responsible for this document is ISO/TC 206, Fine ceramics.
Introduction
This International Standard applies to testing the antiviral activity of indoor-light-active photocatalytic
ceramics and other materials produced by either coating or mixing of a light-active photocatalyst.
The International Standard for testing the antibacterial activity of photocatalytic materials has been
published as ISO 27447 and the International Standard for testing the antibacterial activity of indoor-
light-active photocatalytic materials has been published as ISO 17094. The International Standard for
determination of antiviral activity of semiconducting photocatalytic materials has also been published
as ISO 18061.
The test method for cloths or textiles is not included in this International Standard because of lack of
indoor-light-active photocatalytic cloths or textiles. When the indoor-light-active photocatalytic cloths
or textiles with antiviral activity using indoor-light-active photocatalytic activity have been developed,
a test method for indoor-light-active photocatalytic cloths or textiles will be proposed with the glass
adhesion method in ISO 27447.
vi © ISO 2016 – All rights reserved
INTERNATIONAL STANDARD ISO 18071:2016(E)
Fine ceramics (advanced ceramics, advanced technical
ceramics) — Determination of antiviral activity of
semiconducting photocatalytic materials under
indoor lighting environment — Test method using
bacteriophage Q-beta
WARNING — Only personnel trained in microbiological techniques should carry out tests.
1 Scope
This International Standard
...
INTERNATIONAL ISO
STANDARD 18071
First edition
2016-07-15
Fine ceramics (advanced ceramics,
advanced technical ceramics) —
Determination of antiviral activity
of semiconducting photocatalytic
materials under indoor lighting
environment — Test method using
bacteriophage Q-beta
Céramiques techniques — Détermination de l’activité antivirale
des matériaux photocatalytiques semi-conducteurs dans un
environnement d’éclairage intérieur — Méthode d’essai utilisant un
bactériophage Q-béta
Reference number
©
ISO 2016
© ISO 2016, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
Fax +41 22 749 09 47
copyright@iso.org
www.iso.org
ii © ISO 2016 – All rights reserved
Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols . 2
5 Principle . 3
6 Materials . 3
6.1 Strains and preparation for tests . 3
6.1.1 Strains . 3
6.1.2 Bacteria preparation. 4
6.1.3 Bacteriophage preparation . 4
6.2 Media . 5
6.2.1 General. 5
6.2.2 1/500 Nutrient broth (1/500 NB) . 5
6.2.3 Calcium solution . 5
6.2.4 LB broth with calcium . 5
6.2.5 Agar powder . 5
6.2.6 LB agar . 5
6.2.7 Bottom agar plate (LB agar plate with calcium) . 6
6.2.8 Top agar . 6
6.2.9 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP). 6
6.2.10 Peptone saline solution . 6
7 Apparatus and equipment . 6
7.1 Test equipment . 6
7.2 Cover film . 7
7.3 Moisture preservation glass . 7
7.4 Glass tube or glass rod . 7
7.5 Paper filter . 7
7.6 Light source . 7
7.7 UV sharp cut-off filter . 8
7.7.1 Condition A (under 400 nm cut-off condition) . 8
7.7.2 Condition B (under 380 nm cut-off condition) . 8
7.8 Illuminance meter . 8
7.9 Centrifuge . 8
7.10 Sterilized syringe filter unit . 8
8 Test piece . 8
9 Procedure. 8
9.1 General . 8
9.2 Procedure for preparation of bacteria suspension . 9
9.3 Procedure of preparation of test bacteriophage solution . 9
9.4 Procedure of test for indoor-light-active photocatalytic antiviral activity .10
9.5 Indoor lighting condition .10
9.6 Measurement of titre of bacteriophage .11
10 Calculation .12
10.1 General .12
10.2 Test requirement fulfilment validation .12
10.3 Indoor-light-active photocatalyst antiviral activity value calculation .13
10.4 Antiviral activity value calculation without indoor-light-active photocatalyst .13
11 Test report .14
Bibliography .15
iv © ISO 2016 – All rights reserved
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment,
as well as information about ISO’s adherence to the World Trade Organization (WTO) principles in the
Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html.
The committee responsible for this document is ISO/TC 206, Fine ceramics.
Introduction
This International Standard applies to testing the antiviral activity of indoor-light-active photocatalytic
ceramics and other materials produced by either coating or mixing of a light-active photocatalyst.
The International Standard for testing the antibacterial activity of photocatalytic materials has been
published as ISO 27447 and the International Standard for testing the antibacterial activity of indoor-
light-active photocatalytic materials has been published as ISO 17094. The International Standard for
determination of antiviral activity of semiconducting photocatalytic materials has also been published
as ISO 18061.
The test method for cloths or textiles is not included in this International Standard because of lack of
indoor-light-active photocatalytic cloths or textiles. When the indoor-light-active photocatalytic cloths
or textiles with antiviral activity using indoor-light-active photocatalytic activity have been developed,
a test method for indoor-light-active photocatalytic cloths or textiles will be proposed with the glass
adhesion method in ISO 27447.
vi © ISO 2016 – All rights reserved
INTERNATIONAL STANDARD ISO 18071:2016(E)
Fine ceramics (advanced ceramics, advanced technical
ceramics) — Determination of antiviral activity of
semiconducting photocatalytic materials under
indoor lighting environment — Test method using
bacteriophage Q-beta
WARNING — Only personnel trained in microbiological techniques should carry out tests.
1 Scope
This International Standard
...
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