SIST EN ISO 4833-2:2013/AC:2014

Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating technique - Technical Corrigendum 1 (ISO 4833-2:2013/Cor 1:2014)

This part of ISO 4833 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies on the surface of a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption or for animal feed; b) environmental samples in the area of food and feed production and food handling. This part of ISO 4833 is applicable to: 1) products containing heat-sensitive organisms that are likely to form a significant proportion of the total flora (e.g. psychrotrophic organisms in chilled and frozen foods, dried foods, other foods that may contain heat-sensitive organisms); 2) products containing obligately aerobic bacteria that are likely to form a significant proportion of the total flora (e.g. Pseudomonas spp.); 3) products that contain small particles that can prove difficult to distinguish from colonies in a pour plate; 4) products whose intense colour prevents the recognition of colonies in a pour plate; 5) products for which distinction between different types of colony is required as part of the assessment of food quality. In addition to the manual spread plating technique, this part of ISO 4833 also specifies the use of a spiral plater, a rapid method of performing surface colony counts (Annex A). The applicability of this part of ISO 4833 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in these products are not detected effectively. For some matrices, the method described in this part of ISO 4833 can give different results to those obtained using the method described in ISO 4833-1.

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für die Zählung von Mikroorganismen - Teil 2: Koloniezählung bei 30 °C mittels Oberflächenverfahren - Berichtigung 1 (ISO 4833-2:2013/Cor 1:2014)

Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des micro-organismes - Partie 2: Comptage des colonies à 30 degrés C par la technique d'ensemencement en surface - Rectificatif technique 1 (ISO 4833-2:2013/Cor 1:2014)

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje števila mikroorganizmov - 2. del: Štetje kolonij pri 30 °C s tehniko nasajanja na površino - Tehnični popravek 1 (ISO 4833-2:2013/Cor 1:2014)

Ta del standarda ISO 4833 določa horizontalno metodo za ugotavljanje števila mikroorganizmov, ki lahko rastejo in tvorijo kolonije na trdem mediju po aerobni inkubaciji pri 30 °C. Ta metoda se uporablja za: a) izdelke za prehrano ljudi in živalsko krmo; b) okoljske vzorce v območju proizvodnje in oskrbe s hrano in krmo. Ta del standarda ISO 4833 se uporablja za: 1) izdelke, ki so občutljivi na toploto in za katere se predvideva, da bodo tvorili znaten delež celotne flore (npr. psihotrofni organizmi v ohlajeni in zmrznjeni hrani. suhi hrani, hrani, ki morda vsebuje organizme, občutljive na toploto); 2) izdelke, ki obvezno vsebujejo aerobne bakterije, za katere se predvideva, da bodo tvorile znaten delež celotne flore (npr. Pseudomonas spp.); 3) izdelke, ki vsebujejo majhne delce, ki jih je na petrijevki težko razlikovati od kolonij; 4) izdelke, katerih intenzivna barva preprečuje razpoznavanje kolonij na petrijevki; 5) izdelke, za katere je za oceno kakovosti hrane zahtevano razlikovanje med različnimi vrstami kolonij. Poleg ročne tehnike z uporabo petrijevke ta del standarda ISO 4833 navaja uporabo spiralne petrijevke, ki predstavlja hitro metodo za štetje kolonij na površini (Dodatek A). Uporaba tega dela standarda ISO 4833 za preiskovanje določenih vrst fermentirane hrane in živalske krme je omejena in je za to mogoče bolj primeren drug medij ali inkubacijski pogoji. Toda ta metoda se lahko uporabi za te izdelke, čeprav se glavnih mikroorganizmov v teh izdelkih morda ne da učinkovito zaznati. Pri nekaterih matricah lahko metoda, opisana v temu delu standarda ISO 4833, da drugačne rezultate rezultatov, pridobljenih z metodo, opisano v standardu ISO 4833-1.

General Information

• Status: Published
• Publication Date: 11-Mar-2014
• ICS: 07.100.30 - Food microbiology
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 27-Feb-2014
• Due Date: 04-May-2014
• Completion Date: 12-Mar-2014
• Ref Project: EN ISO 4833-2:2013/AC:2014 - Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating technique - Technical Corrigendum 1 (ISO 4833-2:2013/Cor 1:2014)

Relations

• Corrects: SIST EN ISO 4833-2:2013 - Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating technique (ISO 4833-2:2013)
• Effective Date: 01-Apr-2014

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für die Zählung von Mikroorganismen - Teil 2: Koloniezählung bei 30 °C mittels Oberflächenverfahren - Berichtigung 1 (ISO 4833-2:2013/Cor 1:2014)Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des micro-organismes - Partie 2: Comptage des colonies à 30 degrés C par la technique d'ensemencement en surface - Rectificatif technique 1 (ISO 4833-2:2013/Cor 1:2014)Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating technique - Technical Corrigendum 1 (ISO 4833-2:2013/Cor 1:2014)07.100.30Mikrobiologija živilFood microbiologyICS:Ta slovenski standard je istoveten z:EN ISO 4833-2:2013/AC:2014SIST EN ISO 4833-2:2013/AC:2014en01-april-2014SIST EN ISO 4833-2:2013/AC:2014SLOVENSKI
STANDARD



SIST EN ISO 4833-2:2013/AC:2014



EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN ISO 4833-2:2013/AC
February 2014
Février 2014
Februar 2014 ICS 07.100.30 English version Version Française Deutsche Fassung
Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating technique - Technical Corrigendum 1 (ISO 4833-2:2013/Cor 1:2014) Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrementdes micro-organismes - Partie 2: Comptage des colonies à 30 degrés C par la technique d'ensemencement en surface - Rectificatif technique 1 (ISO 4833-2:2013/Cor 1:2014)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für die Zählung von Mikroorganismen - Teil 2: Koloniezählung bei 30 °C mittels Oberflächenverfahren - Berichtigung 1 (ISO 4833-2:2013/Cor 1:2014) This corrigendum becomes effective on 19 February 2014 for incorporation in the three official language versions of the EN.
Ce corrigendum prendra effet le 19 février 2014 pour incorporation dans les trois versions linguistiques officielles de la EN.
Die Berichtigung tritt am 19. Februar 2014 zur Einarbeitung in die drei offiziellen Sprachfassungen der EN in Kraft.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN
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