07.100.30 - Food microbiology
ICS 07.100.30 Details
Food microbiology
Lebensmittel-Mikrobiologie
Microbiologie alimentaire
Mikrobiologija živil
General Information
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- Standard10 pagesEnglish languagesale 15% off
- Standard10 pagesFrench languagesale 15% off
This document specifies general requirements and gives guidance on microbiological examinations.
It is applicable to:
— the implementation of specific horizontal or vertical International Standards developed by ISO/TC 34/SC 9 or ISO/TC 34/SC 5 for detection or enumeration of microorganisms, named hereafter “specific standards”;
— good laboratory practices for microbiology laboratories testing samples from the food chain;
— guidance for microbiological laboratories testing samples from the food chain on the technical requirements for conforming to ISO/IEC 17025.
The requirements of this general standard supersede corresponding ones in existing specific standards.
Additional instructions for examinations using the polymerase chain reaction (PCR) are specified in ISO 22174.
This document is applicable to examinations for bacteria, yeasts and moulds and can be used, if supplemented with specific guidance, for parasites and viruses. It does not apply to examinations for toxins or other metabolites (e.g. amines) from microorganisms.
This document is applicable to microbiology of the food chain, from primary production stage to food and animal feed products, including the premises where the food or feed production and handling takes place. It is also applicable to the microbiological examination of water where water is used in food production or is regarded as a food in national legislation.
- Standard91 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies general requirements and gives guidance on microbiological examinations.
It is applicable to:
— the implementation of specific horizontal or vertical International Standards developed by ISO/TC 34/SC 9 or ISO/TC 34/SC 5 for detection or enumeration of microorganisms, named hereafter “specific standards”;
— good laboratory practices for microbiology laboratories testing samples from the food chain;
— guidance for microbiological laboratories testing samples from the food chain on the technical requirements for conforming to ISO/IEC 17025.
The requirements of this general standard supersede corresponding ones in existing specific standards.
Additional instructions for examinations using the polymerase chain reaction (PCR) are specified in ISO 22174.
This document is applicable to examinations for bacteria, yeasts and moulds and can be used, if supplemented with specific guidance, for parasites and viruses. It does not apply to examinations for toxins or other metabolites (e.g. amines) from microorganisms.
This document is applicable to microbiology of the food chain, from primary production stage to food and animal feed products, including the premises where the food or feed production and handling takes place. It is also applicable to the microbiological examination of water where water is used in food production or is regarded as a food in national legislation.
- Standard91 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies general requirements and gives guidance on microbiological examinations. It is applicable to: — the implementation of specific horizontal or vertical International Standards developed by ISO/TC 34/SC 9 or ISO/TC 34/SC 5 for detection or enumeration of microorganisms, named hereafter “specific standards”; — good laboratory practices for microbiology laboratories testing samples from the food chain; — guidance for microbiological laboratories testing samples from the food chain on the technical requirements for conforming to ISO/IEC 17025. The requirements of this general standard supersede corresponding ones in existing specific standards. Additional instructions for examinations using the polymerase chain reaction (PCR) are specified in ISO 22174. This document is applicable to examinations for bacteria, yeasts and moulds and can be used, if supplemented with specific guidance, for parasites and viruses. It does not apply to examinations for toxins or other metabolites (e.g. amines) from microorganisms. This document is applicable to microbiology of the food chain, from primary production stage to food and animal feed products, including the premises where the food or feed production and handling takes place. It is also applicable to the microbiological examination of water where water is used in food production or is regarded as a food in national legislation.
- Standard80 pagesEnglish languagesale 15% off
- Standard87 pagesFrench languagesale 15% off
This document specifies the detection of Clostridium (C.) perfringens. This part of ISO 15213 is applicable to:
• products intended for human consumption;
• products intended for animal feeding;
• environmental samples in the area of food and feed production, handling, and;
• samples from the primary production stage.
This technique is intended to be used when the number of colonies sought is expected to be more than 10 per ml or per g of the test sample.
- Technical specification42 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies minimum performance requirements for methods that quantify the food allergens milk, egg, peanut, hazelnut, almond, brazil nut, macadamia nut, cashew, pistachio nut, walnut, pecan nut, lupine, sesame, mustard, soy, celery, fish, molluscs, crustaceans, and wheat in raw and processed foodstuffs. Within the scope of this document, minimum requirements for an LOQ (Limit of Quantification) will be derived from threshold data of allergic consumers. For quantitative antibody-based methods, a normative annex will describe what specific information the method developer needs to deliver and how performance characteristics shall be validated. Regarding PCR and LC-MS/MS, information on performance characteristics are in parts covered by EN 15634-1 and EN 17644. This document does not apply to fragmented or hydrolysed food allergens, such as casein hydrolysates or soy sauce. It also does not apply to methods that deliver qualitative results only. Methods that cover gluten-containing cereals (wheat, rye, and barley) with regard to coeliac disease are covered by EN 17254.
- Standard19 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies minimum performance requirements for methods that quantify the food allergens milk, egg, peanut, hazelnut, almond, brazil nut, macadamia nut, cashew, pistachio nut, walnut, pecan nut, lupine, sesame, mustard, soy, celery, fish, molluscs, crustaceans, and wheat in raw and processed foodstuffs. Within the scope of this document, minimum requirements for an LOQ (Limit of Quantification) will be derived from threshold data of allergic consumers. For quantitative antibody-based methods, a normative annex will describe what specific information the method developer needs to deliver and how performance characteristics shall be validated. Regarding PCR and LC-MS/MS, information on performance characteristics are in parts covered by EN 15634-1 and EN 17644. This document does not apply to fragmented or hydrolysed food allergens, such as casein hydrolysates or soy sauce. It also does not apply to methods that deliver qualitative results only. Methods that cover gluten-containing cereals (wheat, rye, and barley) with regard to coeliac disease are covered by EN 17254.
- Standard19 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies the detection of Clostridium (C.) perfringens.
This document is applicable to:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
NOTE Interlaboratory studies with a small number of participating laboratories (<10) were conducted for the following food categories:
— ready-to-eat, ready-to-reheat meat products;
— eggs and egg products (derivates);
— ready-to-eat, ready-to-reheat fishery products;
— processed fruits and vegetables;
— infant formula and infant cereals (with probiotics);
— multi-component foods or meal components.
It has also been validated with a small number of participating laboratories for the following other category:
— environmental samples (food or feed production).
Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. The method has not been validated for the category ‘pet food and animal feed’, as the test samples used for the interlaboratory study were already naturally contaminated with C. perfringens. Given the limited number of participating laboratories in the interlaboratory studies, the calculated performance characteristics can be used as indicative values of the method performance. For detailed information on the validation, see Clause 11 and Annexes C to F.
- Technical specification42 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies the detection of Clostridium (C.) perfringens. This document is applicable to: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. NOTE Interlaboratory studies with a small number of participating laboratories ( — ready-to-eat, ready-to-reheat meat products; — eggs and egg products (derivates); — ready-to-eat, ready-to-reheat fishery products; — processed fruits and vegetables; — infant formula and infant cereals (with probiotics); — multi-component foods or meal components. It has also been validated with a small number of participating laboratories for the following other category: — environmental samples (food or feed production). Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. The method has not been validated for the category ‘pet food and animal feed’, as the test samples used for the interlaboratory study were already naturally contaminated with C. perfringens. Given the limited number of participating laboratories in the interlaboratory studies, the calculated performance characteristics can be used as indicative values of the method performance. For detailed information on the validation, see Clause 11 and Annexes C to F.
- Technical specification34 pagesEnglish languagesale 15% off
- Technical specification24 pagesFrench languagesale 15% off
- Amendment7 pagesEnglish languagesale 10% offe-Library read for1 day
- Amendment8 pagesEnglish languagesale 10% offe-Library read for1 day
This document gives technical requirements and guidance on the establishment or revision of
standardized reference methods used for the analysis of microorganisms in:
— products intended for human consumption;
— products for feeding animals;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
This document specifies the technical stages of the establishment of a new standardized reference
method and of the revision of an existing standardized reference method. It includes, in particular,
requirements and guidance on the validation of the selected method.
This document is intended to be implemented in particular by ISO/TC 34/SC 9 and its corresponding
structure at CEN level, which is CEN/TC 463.
- Standard20 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies the enumeration of Clostridium (C.) perfringens by colony-count technique.
This document is applicable to:
— products intended for human consumption;
— products for feeding animals;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
NOTE This method has been validated in an interlaboratory study for the following food categories:
— ready-to-eat, ready-to-reheat meat products;
— eggs and egg products (derivates);
— processed fruits and vegetables;
— infant formula and infant cereals;
— multi-component foods or meal components.
It has also been validated for the following other categories:
— pet food and animal feed;
— environmental samples (food or feed production).
As this method has been validated for at least five food categories, this method is applicable for a broad range of
food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly
used for samples in the primary production stage, this category was not included in the interlaboratory study.
Therefore, no performance characteristics were obtained for this category.
This horizontal method was originally developed for the examination of all samples belonging to the
food chain. Based on the information available at the time of publication of this document, this method
is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not
appropriate in every detail for all products. Nevertheless, it is expected that the required modifications
are minimized so that they do not result in a significant deviation from this horizontal method.
This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples
with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is
expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.
- Standard53 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies the enumeration of Clostridium (C.) perfringens by colony-count technique.
This document is applicable to:
— products intended for human consumption;
— products for feeding animals;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
NOTE This method has been validated in an interlaboratory study for the following food categories:
— ready-to-eat, ready-to-reheat meat products;
— eggs and egg products (derivates);
— processed fruits and vegetables;
— infant formula and infant cereals;
— multi-component foods or meal components.
It has also been validated for the following other categories:
— pet food and animal feed;
— environmental samples (food or feed production).
As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category.
This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.
- Standard53 pagesEnglish languagesale 10% offe-Library read for1 day
This document gives technical requirements and guidance on the establishment or revision of standardized reference methods used for the analysis of microorganisms in:
— products intended for human consumption;
— products for feeding animals;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
This document specifies the technical stages of the establishment of a new standardized reference method and of the revision of an existing standardized reference method. It includes, in particular, requirements and guidance on the validation of the selected method.
This document is intended to be implemented in particular by ISO/TC 34/SC 9 and its corresponding structure at CEN level, which is CEN/TC 463.
- Standard20 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies the enumeration of Clostridium (C.) perfringens by colony-count technique. This document is applicable to: — products intended for human consumption; — products for feeding animals; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. NOTE This method has been validated in an interlaboratory study for the following food categories: — ready-to-eat, ready-to-reheat meat products; — eggs and egg products (derivates); — processed fruits and vegetables; — infant formula and infant cereals; — multi-component foods or meal components. It has also been validated for the following other categories: — pet food and animal feed; — environmental samples (food or feed production). As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.
- Standard44 pagesEnglish languagesale 15% off
- Standard45 pagesFrench languagesale 15% off
- Amendment12 pagesEnglish languagesale 10% offe-Library read for1 day
This document gives technical requirements and guidance on the establishment or revision of standardized reference methods used for the analysis of microorganisms in: — products intended for human consumption; — products for feeding animals; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. This document specifies the technical stages of the establishment of a new standardized reference method and of the revision of an existing standardized reference method. It includes, in particular, requirements and guidance on the validation of the selected method. This document is intended to be implemented in particular by ISO/TC 34/SC 9 and its corresponding structure at CEN level, which is CEN/TC 463.
- Standard13 pagesEnglish languagesale 15% off
- Standard13 pagesFrench languagesale 15% off
- Standard2 pagesEnglish languagesale 15% off
- Standard2 pagesFrench languagesale 15% off
- Amendment7 pagesEnglish languagesale 10% offe-Library read for1 day
- Amendment8 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard2 pagesEnglish languagesale 15% off
- Standard2 pagesFrench languagesale 15% off
- Standard1 pageEnglish languagesale 15% off
- Standard1 pageFrench languagesale 15% off
Refers to taper dimensions and tolerances for electrode caps, electrode adaptors, electrode holders and similar parts, where the electrode force Fmax, given for diameter d1 in tables 1, 2 and 3 is not exceeded. Establishes dimensions, designation and marking. Cancels and replaces ISO Recommendation R 1089-1969, of which it constitutes a technical revision.
- Standard15 pagesEnglish languagesale 10% offe-Library read for1 day
- Amendment12 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard6 pagesEnglish languagesale 15% off
- Standard7 pagesFrench languagesale 15% off
- Amendment8 pagesEnglish languagesale 10% offe-Library read for1 day
Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media
- Amendment32 pagesEnglish languagesale 10% offe-Library read for1 day
This method describes a procedure for the qualitative detection of hazelnut (Corylus avellana) in
chocolate. DNA is extracted from the chocolate and a specific DNA sequence for hazelnut
detected from the gene for corA 1.
- Standard16 pagesEnglish languagesale 10% offe-Library read for1 day
Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media
- Amendment30 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies the detection of Clostridium perfringens. This part of ISO 15213 is applicable to:
• products intended for human consumption;
• products intended for animal feeding;
• environmental samples in the area of food and feed production, handling, and
• samples from the primary production stage.
This method is applicable when the number sought is expected to be below 100 per ml or per g of the test sample.
- Standard30 pagesEnglish languagesale 10% offe-Library read for1 day
This method specifies a procedure for the qualitative detection of species specific DNA from
white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex realtime
PCR based on the genes MADS-D (mustard) and lectin (soya). A mustard content of 10
mg/kg or greater and a soya content of 10 mg/kg or greater can be detected with a probability of
> 95 %.
- Standard17 pagesEnglish languagesale 10% offe-Library read for1 day
- Amendment11 pagesEnglish languagesale 10% offe-Library read for1 day
This method describes a procedure for the qualitative detection of peanut (Arachis hypogaea) in
chocolate using real-time PCR based on the gene for the peanut allergen Ara h 2 [4, 5].
- Standard16 pagesEnglish languagesale 10% offe-Library read for1 day
- Amendment11 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the detection of peanut (Arachis hypogaea) in chocolate.
Real-time PCR (Polymerase Chain Reaction) detection of peanut is based on an 86 bp (base pair) sequence from the Ara h 2 gene of peanut.
- Standard16 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a procedure for the qualitative detection of species specific DNA from white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex real-time PCR based on the genes MADS-D (mustard) and lectin (soya).
- Standard17 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies a method for the detection of hazelnut (Corylus avellana) in chocolate.
Real-time PCR (Polymerase chain reaction) detection of hazelnut is based on an152 bp (base pair) sequence from the corA 1 gene of hazelnut.
- Standard16 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard5 pagesEnglish languagesale 15% off
- Standard5 pagesFrench languagesale 15% off
Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media
- Amendment30 pagesEnglish languagesale 10% offe-Library read for1 day
- Amendment8 pagesEnglish languagesale 10% offe-Library read for1 day
Inclusion of methods for molecular confirmation and identification of thermotolerant Campylobacter spp. and change of the performance testing of culture media
- Amendment32 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies the enumeration of sulfite-reducing Clostridium spp. by the colony-count technique.
This document is applicable to:
— products intended for human consumption;
— products for feeding animals;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
NOTE This method has been validated in an interlaboratory study for the following food categories:
— ready-to-eat, ready-to-reheat meat products;
— eggs and egg products (derivates);
— processed fruits and vegetables;
— infant formula and infant cereals;
— multi-component foods or meal components.
It has also been validated for the following other categories:
— pet food and animal feed;
— environmental samples (food or feed production).
As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category.
This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.
- Standard30 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard2 pagesEnglish languagesale 15% off
- Standard2 pagesFrench languagesale 15% off
- Standard24 pagesEnglish languagesale 15% off
- Standard24 pagesFrench languagesale 15% off
- Standard26 pagesEnglish languagesale 15% off
- Standard26 pagesFrench languagesale 15% off
This document specifies the enumeration of sulfite-reducing Clostridium spp. by the colony-count technique. This document is applicable to: — products intended for human consumption; — products for feeding animals; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. NOTE This method has been validated in an interlaboratory study for the following food categories: — ready-to-eat, ready-to-reheat meat products; — eggs and egg products (derivates); — processed fruits and vegetables; — infant formula and infant cereals; — multi-component foods or meal components. It has also been validated for the following other categories: — pet food and animal feed; — environmental samples (food or feed production). As this method has been validated for at least five food categories, this method is applicable for a broad range of food. For detailed information on the validation, see Clause 11 and Annex C. Since the method is not commonly used for samples in the primary production stage, this category was not included in the interlaboratory study. Therefore, no performance characteristics were obtained for this category. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Based on the information available at the time of publication of this document, this method is considered to be fully suited to the examination of all samples belonging to the food chain. However, because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. This technique is suitable for, but not limited to, the enumeration of microorganisms in test samples with a minimum of 10 colonies counted on a plate. This corresponds to a level of contamination that is expected to be higher than 10 cfu/ml for liquid samples or higher than 100 cfu/g for solid samples.
- Standard21 pagesEnglish languagesale 15% off
- Standard22 pagesFrench languagesale 15% off
This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages:
a) handling of bacterial cultures;
b) axenic genomic DNA isolation;
c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
e) metadata capture and sequence repository deposition;
f) validation of the end-to-end WGS workflow (fit for purpose for intended application).
This document is applicable to bacteria isolated from:
— products intended for human consumption;
— products intended for animal feed;
— environmental samples from food and feed handling and production areas;
— samples from the primary production stage.
- Standard54 pagesEnglish languagesale 10% offe-Library read for1 day
This document specifies protocols for conducting microbiological challenge tests for growth studies on vegetative and spore-forming bacteria in raw materials and intermediate or end products.
The use of this document can be extended to yeasts that do not form mycelium.
- Standard32 pagesEnglish languagesale 10% offe-Library read for1 day
- Standard32 pagesEnglish languagesale 10% offe-Library read for1 day