SIST EN ISO 7218:2007
(Main)Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations (ISO 7218:2007)
Microbiology of food and animal feeding stuffs - General requirements and guidance for microbiological examinations (ISO 7218:2007)
This International Standard gives general requirements and guidance/options intended for three main uses: - implementation of ISO/TC 34/SC 9 or ISO/TC 34/SC 5 standards for detection or enumeration of microorganisms, named hereafter "specific standards"; - good laboratory practice for food microbiological laboratories (the purpose is not to detail them in this International Standard, manuals are available for that purpose); - guidance for accreditation of food microbiological laboratories (this International Standard describes the technical requirements according to Annex B of ISO/IEC 17025:2005 for the accreditation of a microbiological laboratory by national organizations). The requirements of this International Standard supersede the corresponding ones of existing specific standards.
Mikrobiologie von Lebensmitteln und Futtermitteln - Allgemeine Anforderungen und Anleitung für mikrobiologische Untersuchungen (ISO 7218:2007)
Diese Internationale Norm legt allgemeine Anforderungen fest und gibt eine Anleitung/Wahl¬möglichkeiten für die drei wesentlichen Anwendungen:
praktische Anwendung der Normen des ISO TC 34/SC 9 oder des ISO TC 34/SC 5 für den Nachweis oder die Zählung von Mikroorganismen, im Folgenden "spezifische Normen" genannt;
Umsetzung der Guten Laborpraxis in Laboratorien der Lebensmittelmikrobiologie (der Zweck besteht nicht darin, diese in dieser Norm ausführlich zu beschreiben; hierfür stehen Handbücher zur Verfügung);
Anleitung zur Akkreditierung von Laboratorien der Lebensmittelmikrobiologie (diese Internationale Norm beschreibt die Technischen Anforderungen nach Anhang B von ISO/IEC 17025:2005 für die Akkreditierung eines mikrobiologischen Labors durch nationale Organisationen).
Die Anforderungen dieser Internationalen Norm ersetzen die in den bestehenden spezifischen Normen enthaltenen entsprechenden Vorschriften.
Zusätzliche Anweisungen für den Bereich der molekularbiologischen Untersuchungen sind in ISO 22174 festgelegt.
Diese Internationale Norm erstreckt sich auf die Untersuchung von Bakterien, Hefen und Schimmelpilzen und kann bei entsprechender Ergänzung um die spezifischen Anleitungen auf Prionen, Parasiten und Viren angewendet werden. Diese Internationale Norm umfasst nicht die Untersuchung von Toxinen oder sonstigen Stoffwechselprodukten (z. B. Amine) von Mikroorganismen.
Diese Internationale Norm gilt für die Mikrobiologie von Lebens und Futtermitteln sowie für den Bereich der Herstellung von Lebensmitteln und für die Primärproduktion.
Zweck dieser Internationalen Norm ist es, zur Sicherstellung der Validität der lebensmittelmikrobiologischen Untersuchungen und der Gleichartigkeit der bei diesen Untersuchungen angewandten allgemeinen Techniken in allen Laboratorien beizutragen.
Microbiologie des aliments - Exigences générales et recommendations (ISO 7218:2007)
L'ISO 7218:2006 spécifie des exigences générales et des recommandations/options visant trois utilisations principales:
l'application des normes élaborées par l'ISO TC 34/SC 9 ou l'ISO TC 34/SC 5 pour la détection ou le dénombrement des micro-organismes;les bonnes pratiques de laboratoire pour les laboratoires de microbiologie alimentaire (la présente Norme internationale ne vise pas à les détailler, des manuels sont disponibles à cet effet);les recommandations relatives à l'agrément des laboratoires de microbiologie alimentaire (la présente Norme internationale décrit les exigences techniques conformes à l'Annexe B de l'ISO/CEI 17025:2005 relative à l'accréditation d'un laboratoire de microbiologie par les organismes nationaux).
L'ISO 7218:2006 couvre l'examen pour les bactéries, les levures et les moisissures et peut être utilisée si elle est complétée par des recommandations spécifiques sur les prions, les parasites et les virus. Elle ne traite pas de l'examen des toxines ou autres métabolites (par exemple les amines) provenant de micro-organismes.
Elle s'applique à la microbiologie des aliments, des aliments pour animaux, à l'environnement de production d'aliments et l'environnement de production primaire.
L'objet de l'ISO 7218:2006 est d'aider à garantir la validité des examens microbiologiques des aliments, s'assurer que les méthodes générales utilisées pour effectuer ces examens sont les mêmes dans tous les laboratoires, à participer ainsi à l'obtention de résultats homogènes dans les différents laboratoires et à contribuer à la sécurité du personnel de laboratoire, en prévenant les risques d'infection.
Mikrobiologija živil in krme - Splošna pravila za mikrobiološke preiskave (ISO 7218:2007)
Ta mednarodni standard opredeljuje splošne zahteve in navodila/možnosti za tri glavne načine uporabe: – izvajanje standardov ISO/TC 34/SC 9 ali ISO/TC 34/SC 5 za odkrivanje in štetje mikroorganizmov, v nadaljnjem besedilu »posebni standardi«, – dobra laboratorijska praksa za mikrobiološke laboratorije za preiskave živil (teh ta mednarodni standard ne opredeljuje, za to so na voljo priročniki); –navodila za akreditacijo mikrobioloških laboratorijev za preiskave živil (ta mednarodni standard opisuje tehnične zahteve iz Dodatka B standarda ISO/IEC 17025:2005 za akreditacijo mikrobiološkega laboratorija s strani državnih organizacij). Zahteve tega mednarodnega standarda nadomeščajo ustrezne zahteve obstoječih posebnih standardov.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-november-2007
Mikrobiologija živil in krmil - Splošna pravila za mikrobiološke preiskave (ISO
7218:2007)
Microbiology of food and animal feeding stuffs - General requirements and guidance for
microbiological examinations (ISO 7218:2007)
Mikrobiologie von Lebensmitteln und Futtermitteln - Allgemeine Anforderungen und
Anleitung für mikrobiologische Untersuchungen (ISO 7218:2007)
Microbiologie des aliments - Exigences générales et recommendations (ISO 7218:2007)
Ta slovenski standard je istoveten z: EN ISO 7218:2007
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 7218
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2007
ICS 07.100.30
English Version
Microbiology of food and animal feeding stuffs - General
requirements and guidance for microbiological examinations
(ISO 7218:2007)
Microbiologie des aliments - Exigences générales et Mikrobiologie von Lebensmitteln und Futtermitteln -
recommendations (ISO 7218:2007) Allgemeine Anforderungen und Anleitung für
mikrobiologische Untersuchungen (ISO 7218:2007)
This European Standard was approved by CEN on 19 April 2007.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 7218:2007: E
worldwide for CEN national Members.
Contents Page
Foreword.3
Foreword
This document (EN ISO 7218:2007) has been prepared by Technical Committee ISO/TC 34 "Agricultural food
products" in collaboration with Technical Committee CEN/TC 275 "Food analysis - Horizontal methods" the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2008, and conflicting national standards shall be withdrawn
at the latest by February 2008.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 7218:2007 has been approved by CEN as a EN ISO 7218:2007 without any modification.
INTERNATIONAL ISO
STANDARD 7218
Third edition
2007-08-15
Microbiology of food and animal feeding
stuffs — General requirements and
guidance for microbiological
examinations
Microbiologie des aliments — Exigences générales et
recommandations
Reference number
ISO 7218:2007(E)
©
ISO 2007
ISO 7218:2007(E)
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ISO 7218:2007(E)
Contents Page
Foreword. v
Introduction . vi
1 Scope . 1
2 Normative references . 1
3 Premises . 2
3.1 General. 2
3.2 Safety considerations. 2
3.3 Laboratory design. 2
3.4 Laboratory areas. 3
3.5 Layout and fittings of the premises. 4
3.6 Cleaning and disinfection . 5
4 Staff . 5
4.1 General. 5
4.2 Competence . 6
4.3 Verification of on-going staff competence. 6
4.4 Hygiene . 6
5 Apparatus and equipment . 6
6 Preparation of glassware and other laboratory materials. 28
6.1 Preparation . 28
6.2 Sterilization/decontamination. 28
6.3 Disposable equipment and materials . 28
6.4 Storage of clean glassware and materials . 28
6.5 Management of sterile glassware and materials . 29
6.6 Use of decontamination and disinfection . 29
6.7 Waste management . 29
6.8 Washing . 30
7 Preparation and sterilization of culture media . 30
8 Laboratory samples. 30
8.1 Sampling. 30
8.2 Transport . 31
8.3 Receipt . 31
8.4 Storage. 32
8.5 Test portion . 32
9 Examination. 32
9.1 Hygienic precautions during analysis. 32
9.2 Preparation of initial suspension and dilutions . 34
10 Enumeration . 34
10.1 General. 34
10.2 Enumeration using a solid medium. 35
10.3 Calculation and expression of results obtained with solid media . 37
10.4 Enumeration of yeasts and moulds. 43
10.5 Enumeration using a liquid medium. 44
11 Detection method (qualitative method) . 49
11.1 General. 49
11.2 Principle. 50
11.3 Measurement of uncertainty. 50
ISO 7218:2007(E)
12 Confirmatory methods. 50
12.1 General . 50
12.2 Preparation of a pure culture. 50
12.3 Gram’s stain (modified Hucker technique). 50
12.4 Use of biochemical galleries for identification . 52
12.5 Use of nucleic probes for identification . 52
12.6 Serological methods. 53
13 Test report. 53
14 Validation of microbiological methods. 54
14.1 Validation of reference methods . 54
14.2 Validation of alternative methods. 54
14.3 Validation of in-house methods. 54
15 Quality assurance of results/quality control of performance.
...
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