SIST EN ISO 21872-1:2017/A1:2023

Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus - Amendment 1: Inclusion of performance testing of culture media and reagents (ISO 21872 1:2017/Amd 1:2023)

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zur Bestimmung von Vibrio spp. - Teil 1: Nachweis von potentiell enteropathenogenen Vibrio parahaemolyticus, Vibrio cholerae und Vibrio vulnificus - Änderung 1 (ISO 21872 1:2017/Amd 1:2023)

No scope available

Microbiologie de la chaîne alimentaire - Méthode horizontale pour la détermination des Vibrio spp. - Partie 1: Recherche des espèces de Vibrio parahaemolyticus, Vibrio cholerae et Vibrio vulnificus potentiellement entéropathogènes - Amendement 1: Inclusion des essais de performance des milieux de culture et réactifs (ISO 21872 1:2017/Amd 1:2023)

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje Vibrio spp. - 1. del: Ugotavljanje potencialno enteropatogene Vibrio parahaemolyticus, Vibrio cholerae in Vibrio vulnificus - Dopolnilo A1: Vključitev preskušanja učinkovitosti gojišč in reagentov (ISO 21872 1:2017/Amd 1:2023)

General Information

• Status: Published
• Public Enquiry End Date: 14-Oct-2021
• Publication Date: 05-Apr-2023
• ICS: 07.100.30 - Food microbiology
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 29-Mar-2023
• Due Date: 03-Jun-2023
• Completion Date: 06-Apr-2023
• Ref Project: EN ISO 21872-1:2017/A1:2023 - Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus - Amendment 1: Inclusion of performance testing of culture media and reagents (ISO 21872 1:2017/Amd 1:2023)

Relations

• Amends: SIST EN ISO 21872-1:2017 - Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)
• Effective Date: 01-Oct-2021

Overview

EN ISO 21872-1:2017/A1:2023 is an amendment to the international/CEN standard for the microbiology of the food chain. It covers the horizontal method for the detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus and adds explicit requirements for performance testing of culture media and reagents. The amendment aligns media quality assurance with ISO 11133 and supplies additional guidance in Annex B (Clause B.12).

Key Topics

• Amendment focus: Inclusion of performance testing procedures for culture media and reagents to support reliable detection of Vibrio spp.
• Normative linkage: Performance testing is to be carried out in accordance with ISO 11133 and the new Clause B.12 in this amendment.
• Annex B additions: Clause B.12 defines the approach to performance testing and references two new tables (Table B.1 and Table B.2) that guide quality assurance.
• Performance tables:
  • Table B.1 details performance testing for selective and non-selective culture media (examples in the tables include TCBS, saline peptone water (ASPW) and saline nutrient agar (SNA)), listing control strains and qualitative productivity/selectivity criteria.
  • Table B.2 covers confirmation media and reagent performance (examples include arginine dihydrolase, indole, oxidase, L-lysine decarboxylase, halotolerance/NaCl tests and β-galactosidase), with suggested control strains and expected positive/negative reactions.
• Control strains and traceability: The amendment references World Data Centre for Microorganisms (WDCM) strain numbers and notes national restrictions that may apply to use, storage or transport of some strains (for example V. cholerae).

Applications

• Food microbiology laboratories validating or accrediting Vibrio detection workflows.
• Quality assurance teams performing routine performance testing of culture media and reagent lots.
• Public health and regulatory testing where standardized detection methods and media validation reduce false negatives and improve inter-laboratory comparability.
• Aquaculture and seafood testing to support safe supply chain monitoring.

Practical benefits include clearer acceptance criteria for media, improved reproducibility of Vibrio detection, and harmonised procedures for media performance testing when used together with ISO 11133.

Related Standards

• ISO 21872-1:2017 (original Part 1 standard for Vibrio detection)
• ISO 11133 (performance testing of culture media and reagents)
• CEN/TC 463 and ISO/TC 34 (technical committees responsible for microbiology of the food chain)

For implementation, laboratories should apply the procedures in ISO 11133 and follow Clause B.12 and the annex tables to ensure media and reagent performance meets the criteria specified by EN ISO 21872-1:2017/A1:2023.

Standards Content (Sample)

SLOVENSKI STANDARD
01-maj-2023
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje Vibrio
spp. - 1. del: Ugotavljanje potencialno enteropatogene Vibrio parahaemolyticus,
Vibrio cholerae in Vibrio vulnificus - Dopolnilo A1: Vključitev preskušanja
učinkovitosti gojišč in reagentov (ISO 21872 1:2017/Amd 1:2023)
Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. -
Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio
cholerae and Vibrio vulnificus - Amendment 1: Inclusion of performance testing of culture
media and reagents (ISO 21872 1:2017/Amd 1:2023)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zur Bestimmung von Vibrio
spp. - Teil 1: Nachweis von potentiell enteropathenogenen Vibrio parahaemolyticus,
Vibrio cholerae und Vibrio vulnificus - Änderung 1 (ISO 21872 1:2017/Amd 1:2023)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la détermination des
Vibrio spp. - Partie 1: Recherche des espèces de Vibrio parahaemolyticus, Vibrio
cholerae et Vibrio vulnificus potentiellement entéropathogènes - Amendement 1:
Inclusion des essais de performance des milieux de culture et réactifs (ISO 21872
1:2017/Amd 1:2023)
Ta slovenski standard je istoveten z: EN ISO 21872-1:2017/A1:2023
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 21872-1:2017/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2023
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Horizontal method for the
determination of Vibrio spp. - Part 1: Detection of
potentially enteropathogenic Vibrio parahaemolyticus,
Vibrio cholerae and Vibrio vulnificus - Amendment 1:
Inclusion of performance testing of culture media and
reagents (ISO 21872 1:2017/Amd 1:2023)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour la détermination des Vibrio spp. - Verfahren zur Bestimmung von Vibrio spp. - Teil 1:
Partie 1: Recherche des espèces de Vibrio Nachweis von potentiell enteropathenogenen Vibrio
parahaemolyticus, Vibrio cholerae et Vibrio vulnificus parahaemolyticus, Vibrio cholerae und Vibrio
potentiellement entéropathogènes - Amendement 1: vulnificus - Änderung 1 (ISO 21872 1:2017/Amd
Inclusion des essais de performance des milieux de 1:2023)
culture et réactifs (ISO 21872 1:2017/Amd 1:2023)
This amendment A1 modifies the European Standard EN ISO 21872-1:2017; it was approved by CEN on 24 December 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21872-1:2017/A1:2023 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 21872-1:2017/A1:2023) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food
chain” the secretariat of which is held by AFNOR.
This Amendment to the European Standard EN ISO 21872-1:2017 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by September 2023,
and conflicting national standards shall be withdrawn at the latest by September 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 21872-1:2017/Amd 1:2023 has been approved by CEN as EN ISO 21872-
1:2017/A1:2023 without any modification.

INTERNATIONAL ISO
STANDARD 21872-1
First edition
2017-06
AMENDMENT 1
2023-02
Microbiology of the food chain —
Horizontal method for the
determination of Vibrio spp. —
Part 1:
Detection of potentially
enteropathogenic Vibrio
parahaemolyticus, Vibrio cholerae
and Vibrio vulnificus
AMENDMENT 1: Inclusion of
performance testing of culture media
and reagents
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la détermination des Vibrio spp. —
Partie 1: Recherche des espèces de Vibrio parahaemolyticus, Vibrio
cholerae et Vibrio vulnificus potentiellement entéropathogènes
AMENDEMENT 1: Inclusion des essais de performance des milieux de
culture et réactifs
Reference number
ISO 21872-1:2017/Amd.1:2023(E)
ISO 21872-1:2017/Amd.1:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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Published in Switzerland
ii
ISO 21872-1:2017/Amd.1:2023(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
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