ASTM D2330-02
(Test Method)Standard Test Method for Methylene Blue Active Substances (Withdrawn 2011)
Standard Test Method for Methylene Blue Active Substances (Withdrawn 2011)
SIGNIFICANCE AND USE
The widespread use and discharge of detergents into surface waters can result in a lowering of its aesthetic quality by foam formation and by causing toxicity to aquatic wildlife. This test method is capable of detecting small concentrations of detergents as MBAS so that they can be controlled to prevent such problems.
Biodegradable linear alkyl benzene sulfonates (LAS) have replaced the branched-chain alkyl benzene sulfonates (ABS) in detergent formulations, which were more resistant to biodegradation. Differentiation between linear and branched-chain alkyl benzene sulfonates, as well as differentiation of the various positional isomers of either type, is not possible by this test method. While the methylene blue method may be employed to monitor studies designed to measure biodegradability, it cannot be used to predict this quality.
SCOPE
1.1 This test method covers the determination of compounds that react with methylene blue under the conditions specified in the test procedure. They are referred to as methylene blue active substances (MBAS), and are calculated and reported in terms of the reference material, linear alkyl benzene sulfonate, LAS.
1.2 This test method is applicable for determining MBAS in water and wastewater. It is the user's responsibility to ensure the validity of this test method for waters of untested matrices.
1.3 This test method is a simple, rapid, control procedure suitable for monitoring the effectiveness of a biodegradation or other linear alkyl benzene sulfonate (LAS) removal process. For greater specificity and interference removal, the pretreatment procedure in should be used. Data derived without the pretreatment procedure should be interpreted with care. This test method is applicable in the range from 0.03 to 1.5 mg/L for a 100-mL sample.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For a specific hazard statement, see 8.3.
WITHDRAWN RATIONALE
Formerly under the jurisdiction of Committee D19 on Water, this Test Method was withdrawn in 2011 in accordance with section 10.5.3.1 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.
General Information
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Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:D2330–02
Standard Test Method for
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Methylene Blue Active Substances
This standard is issued under the fixed designation D2330; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
This standard has been approved for use by agencies of the Department of Defense.
1. Scope D4210 Practice for Intralaboratory Quality Control Proce-
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dures and a Discussion on Reporting Low-Level Data
1.1 This test method covers the determination of com-
D5788 Guide for Spiking Organics into Aqueous Samples
pounds that react with methylene blue under the conditions
D5789 Practice for Writing Quality Control Specifications
specified in the test procedure. They are referred to as
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for Standard Test Methods for Organic Constituents
methylene blue active substances (MBAS), and are calculated
D5847 Practice for Writing Quality Control Specifications
and reported in terms of the reference material, linear alkyl
for Standard Test Methods for Water Analysis
benzene sulfonate, LAS.
E60 Practice for Analysis of Metals, Ores, and Related
1.2 ThistestmethodisapplicablefordeterminingMBASin
Materials by Molecular Absorption Spectrometry
water and wastewater. It is the user’s responsibility to ensure
E131 Terminology Relating to Molecular Spectroscopy
the validity of this test method for waters of untested matrices.
E275 Practice for Describing and Measuring Performance
1.3 This test method is a simple, rapid, control procedure
of Ultraviolet and Visible Spectrophotometers
suitableformonitoringtheeffectivenessofabiodegradationor
other linear alkyl benzene sulfonate (LAS) removal process.
3. Terminology
For greater specificity and interference removal, the pretreat-
3.1 Definitions—For definitions of terms used in this test
ment procedure in Annex A1 should be used. Data derived
method, refer to Terminology D1129 and E131.
without the pretreatment procedure should be interpreted with
3.2 Definitions of Terms Specific to This Standard:
care. This test method is applicable in the range from 0.03 to
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3.2.1 alkyl benzene sulfonate (ABS) —the generic name
1.5 mg/L for a 100-mL sample.
applied to the neutralized product resulting from the sulfona-
1.4 This standard does not purport to address all of the
tion of a branched-chain alkylated benzene. See also Termi-
safety concerns, if any, associated with its use. It is the
nology D459.
responsibility of the user of this standard to establish appro-
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3.2.2 linear alkyl benzene sulfonate (LAS) —aformofalkyl
priate safety and health practices and determine the applica-
benzene sulfonate (ABS) in which the alkyl group is linear
bility of regulatory limitations prior to use. For a specific
rather than a branched chain. See also Terminology D459.
hazard statement, see 8.3.
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4. Summary of Test Method
2. Referenced Documents
2 4.1 This test method is based upon the formation of a
2.1 ASTM Standards:
blue-colored chloroform extractable ion pair by the reaction of
D459 Terminology Relating to Soaps and Other Detergents
cationic methylene blue and an anionic surfactant (including
D1129 Terminology Relating to Water
LAS, other sulfonates, and sulfate esters).
D1193 Specification for Reagent Water
4.2 Thesampleismixedwithanacidified,aqueoussolution
D3370 Practices for Sampling Water from Closed Conduits
of methylene blue. Any resulting hydrophobic ion pair which
D3856 Guide for Good Laboratory Practices in Laborato-
may be formed is extracted successfully with chloroform. The
ries Engaged in Sampling and Analysis of Water
combinedchloroformextractsarewashedwithanacidsolution
to remove the less hydrophobic ion pairs (having low partition
coefficients) that can be formed by potentially interfering
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This test method is under the jurisdiction ofASTM Committee D19 on Water
andisthedirectresponsibilityofSubcommitteeD19.06onMethodsforAnalysisfor
Organic Substances in Water.
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Current edition approved July 10, 2002. Published August 2002. Originally Withdrawn. The last approved version of this historical standard is referenced
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published as D2330–65T. Last previous edition D2330–88 (1995) . DOI: on www.astm.org.
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10.1520/D2330-02. For a more complete discussion of terms relating to synthetic detergents and
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For referenced ASTM standards, visit the ASTM website, www.astm.org, or their significance, refer to “Syndets and Waste Disposal” by McKinney, R. E.,
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Sewage and Industrial Wastes, Vol 29, Part 6, June 1957, pp. 654–666.
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Standards volume information, refer to the standard’s Document Summary page on Adopted from “Surfactants (Anionic) M
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