ASTM D7574-16

Standard Test Method for Determination of Bisphenol A in Environmental Waters by Liquid Chromatography/Tandem Mass Spectrometry

SIGNIFICANCE AND USE
5.1 The first reported synthesis of BPA was by the reaction of phenol with acetone by Zincke.4 BPA has become an important high volume industrial chemical used in the manufacture of polycarbonate plastic and epoxy resins. Polycarbonate plastic and resins are used in numerous products including electrical and electronic equipment, automobiles, sports and safety equipment, reusable food and drink containers, electrical laminates for printed circuit boards, composites, paints, adhesives, dental sealants, protective coatings and many other products.5  
5.2 The environmental source of BPA is predominantly from the decomposition of polycarbonate plastics and resins. BPA is not classified as bio-accumulative by the U.S. Environmental Protection Agency and will biodegrade. BPA has been reported to have adverse effects in aquatic organisms and may be released into environmental waters directly at trace levels through landfill leachate and POTW effluents. This method has been investigated for use with surface water and secondary and tertiary POTW effluent samples therefore, it is applicable to these matrices only. It has not been investigated for use with salt water or solid sample matrices.
SCOPE
1.1 This procedure covers the determination of bisphenol A (BPA) extracted from water utilizing solid phase extraction (SPE), separated using liquid chromatography (LC) and detected with tandem mass spectrometry (MS/MS). BPA is qualitatively and quantitatively determined by this method. This method adheres to multiple reaction monitoring (MRM) mass spectrometry.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 The method detection limit (MDL) and reporting limit (RL) for BPA are listed in Table 1. (A) MDL determined following the Code of Federal Regulations, 40CFR Part 136, Appendix B.(B) Lowest point of the reporting range, reporting limit, is calculated from the LV 1 concentration calibration standard in Table 4. Fig. 1 displays the signal/noise ratio at the reporting limit.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 31-Jan-2016
• ICS: 13.060.50 - Examination of water for chemical substances
• Technical Committee: D19 - Water
• Drafting Committee: D19.06 - Methods for Analysis for Organic Substances in Water

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D7574 − 16
Standard Test Method for
Determination of Bisphenol A in Environmental Waters by
1
Liquid Chromatography/Tandem Mass Spectrometry
This standard is issued under the fixed designation D7574; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
3
1. Scope 2.2 Other Documents:
The Code of Federal Regulations 40 CFR Part 136, Appen-
1.1 This procedure covers the determination of bisphenol A
dix B
(BPA) extracted from water utilizing solid phase extraction
(SPE), separated using liquid chromatography (LC) and de-
3. Terminology
tected with tandem mass spectrometry (MS/MS). BPA is
3.1 Definitions:
qualitatively and quantitatively determined by this method.
3.1.1 For definitions of terms used in this standard, refer to
This method adheres to multiple reaction monitoring (MRM)
Terminology D1129.
mass spectrometry.
3.2 Definitions of Terms Specific to This Standard:
1.2 The values stated in SI units are to be regarded as
3.2.1 environmental water, n—shall refer to water tested
standard. No other units of measurement are included in this
using this method. See Section 5.
standard.
3.2.2 independent reference material, IRM, n—a material of
1.3 The method detection limit (MDL) and reporting limit
known purity and concentration obtained either from the
(RL) for BPA are listed in Table 1.
National Institute of Standards and Technology (NIST) or other
1.4 This standard does not purport to address all of the
reputable supplier. The IRM shall be obtained from a different
safety concerns, if any, associated with its use. It is the
lot of material than is used for calibration.
responsibility of the user of this standard to establish appro-
3.3 Acronyms:
priate safety and health practices and determine the applica-
3.3.1 CCC, n—Continuing Calibration Check
bility of regulatory limitations prior to use.
3.3.2 IC, n—Initial Calibration
2. Referenced Documents
3.3.3 LC, n—Liquid Chromatography
2
2.1 ASTM Standards:
3.3.4 LCS/LCSD, n—Laboratory Control Sample/
D1129 Terminology Relating to Water
Laboratory Control Sample Duplicate
D1193 Specification for Reagent Water
3.3.5 MDL, n—Method Detection Limit
D2777 Practice for Determination of Precision and Bias of
3.3.6 MeOH, n—Methanol
Applicable Test Methods of Committee D19 on Water
-3
D3694 Practices for Preparation of Sample Containers and 3.3.7 mM, n—millimolar, 1 × 10 moles/L
for Preservation of Organic Constituents
3.3.8 MRM, n—Multiple Reaction Monitoring
D3856 Guide for Management Systems in Laboratories
3.3.9 MS/MSD, n—Matrix Spike/Matrix Spike Duplicate
Engaged in Analysis of Water
3.3.10 NA, adj—Not Available
D5847 Practice for Writing Quality Control Specifications
for Standard Test Methods for Water Analysis 3.3.11 ND, n—non-detect
D5905 Practice for the Preparation of Substitute Wastewater
3.3.12 P&A, n—Precision and Accuracy
3.3.13 PPB, n—parts per billion
1
This test method is under the jurisdiction of ASTM Committee D19 on Water
3.3.14 PPT, n—parts per trillion
and is the direct responsibility of Subcommittee D19.06 on Methods for Analysis for
3.3.15 QA, adj—Quality Assurance
Organic Substances in Water.
Current edition approved Feb. 1, 2016. Published March 2016. Originally
3.3.16 QC, adj—Quality Control
approved in 2009. Last previous edition approved in 2009 as D7574 – 09. DOI:
10.1520/D7574-16.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
3
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Available from U.S. Government Printing Office Superintendent of Documents,
Standards volume information, refer to the standard’s Document Summary page on 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
the ASTM website. www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D7574 − 16
TABLE 1 MDL and Reporting Range
mental Protection Agency and will biodegrade. BPA has been
A B
Analyte MDL (ng/L) Reporting Range (ng/L) reported to have adverse effects in aquatic organisms and may
Bisphenol A 5 20–600
be released into environmental waters directly at trace levels
A
MDL determined following the Code of Federal Regulations, 40CFR Part 136,
through landfill leachate and POTW effluents. This method has
Appendix B.
been investigated for use with surface water and secondary and
B
Lowest point of the reporting range, reporting limit, is calculated from the LV 1
tertiary POTW effluent samples therefore, it is applicable to
concentration calibra
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: D7574 − 09 D7574 − 16
Standard Test Method for
Determination of Bisphenol A in Environmental Waters by
1
Liquid Chromatography/Tandem Mass Spectrometry
This standard is issued under the fixed designation D7574; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This procedure covers the determination of bisphenol A (BPA) extracted from water utilizing solid phase extraction (SPE),
separated using liquid chromatography (LC) and detected with tandem mass spectrometry (MS/MS). BPA is qualitatively and
quantitatively determined by this method. This method adheres to multiple reaction monitoring (MRM) mass spectrometry.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 The method detection limit (MDL) and reporting limit (RL) for BPA are listed in Table 1.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
D2777 Practice for Determination of Precision and Bias of Applicable Test Methods of Committee D19 on Water
D3694 Practices for Preparation of Sample Containers and for Preservation of Organic Constituents
D3856 Guide for Management Systems in Laboratories Engaged in Analysis of Water
D5847 Practice for Writing Quality Control Specifications for Standard Test Methods for Water Analysis
D5905 Practice for the Preparation of Substitute Wastewater
3
2.2 Other Documents:
The Code of Federal Regulations 40 CFR Part 136, Appendix B
3. Terminology
3.1 Definitions:
3.1.1 For definitions of terms used in this standard, refer to Terminology D1129.
3.2 Definitions:Definitions of Terms Specific to This Standard:
3.2.1 environmental water, n—effluents from secondary and tertiary publicly owned treatment works (POTW),shall refer to
water tested using this method. See Section 5fresh surface and ground water.
3.2.2 independent reference material, IRM, n—a material of known purity and concentration obtained either from the National
Institute of Standards and Technology (NIST) or other reputable supplier. The IRM shall be obtained from a different lot of material
than is used for calibration.
3.3 Abbreviations:Acronyms:
3.3.1 CCC, n—Continuing Calibration Check
1
This test method is under the jurisdiction of ASTM Committee D19 on Water and is the direct responsibility of Subcommittee D19.06 on Methods for Analysis for
Organic Substances in Water.
Current edition approved Oct. 1, 2009Feb. 1, 2016. Published October 2009March 2016. Originally approved in 2009. Last previous edition approved in 2009 as D7574
– 09. DOI: 10.1520/D7574-09.10.1520/D7574-16.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401, http://
www.access.gpo.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D7574 − 16
TABLE 1 MDL and Reporting Range
A B
Analyte MDL (ng/L) Reporting Range (ng/L)
Bisphenol A 5 20–600
A
MDL determined following the Code of Federal Regulations, 40CFR Part 136,
Appendix B.
B
Lowest point of the reporting range, reporting limit, is calculated from the LV 1
concentration calibration standard in Table 4. Fig. 1 displays the signal/noise ratio
at the reporting limit.
3.3.2 IC, n—Initial Calibration
3.3.3 LC, n—Liquid Chromatography
3.3.4 LCS/LCSD, n—Laboratory Control Sample/Laboratory Control Sample Duplicate
3.3.5 MDL, n—Method Detection Limit
3.3.6 MeOH, n—Methanol
-3
3.3.7 mM, n—millimolar, 1 × 10 moles/L
3.3.8 MRM, n—Multiple Reaction Monitoring
3.3.9 MS/MSD, n—Matrix Spike/Matrix Spike Duplicate
3.3.10 NA, adj—Not Available
3.3.11 ND, n—non-detect
3
...