ASTM E654-96(2003)
(Test Method)Standard Test Method for Effectiveness of Aerosol and Pressurized Spray Insecticides Against Cockroaches
Standard Test Method for Effectiveness of Aerosol and Pressurized Spray Insecticides Against Cockroaches
SIGNIFICANCE AND USE
This test method provides a satisfactory means of determining the relative efficiency of aerosol formulations when applied as direct sprays to cockroaches. It is not designed to measure the residual action.
As a biological test, it is subject to the variations that accompany the reactions of living organisms. It should be employed under the supervision of personnel familiar with the biological testing of insecticides.
In order to measure the effectiveness with reasonable tolerance, the test is run in conjunction with the Official Test Aerosol II (OTA II)2 as the standard basis of comparison. Another standard that may be used with this test method is the Tentative Official Aqueous Pressurized Spray (TOAPS).2
SCOPE
1.1 This test method covers the determination of the effectiveness of aerosol and pressurized spray insecticides against cockroaches.
1.2 Test data by this test method may also be adequate to support claims for use of the product to control the exposed or accessible stages of silverfish, ants, centipedes, spiders, and certain stored product pests.
1.3 The values stated in inch-pound units are to be regarded as the standard. The values given in parentheses are for information only.
1.4 This standard does not purport to address all of the safety concerns, if any associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation:E654–96 (Reapproved 2003)
Standard Test Method for
Effectiveness of Aerosol and Pressurized Spray Insecticides
Against Cockroaches
This standard is issued under the fixed designation E 654; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope 3.2 As a biological test, it is subject to the variations that
accompany the reactions of living organisms. It should be
1.1 This test method covers the determination of the effec-
employed under the supervision of personnel familiar with the
tiveness of aerosol and pressurized spray insecticides against
biological testing of insecticides.
cockroaches.
3.3 In order to measure the effectiveness with reasonable
1.2 Test data by this test method may also be adequate to
tolerance, the test is run in conjunction with the Official Test
support claims for use of the product to control the exposed or
Aerosol II (OTA II) as the standard basis of comparison.
accessible stages of silverfish, ants, centipedes, spiders, and
Another standard that may be used with this test method is the
certain stored product pests.
Tentative Official Aqueous Pressurized Spray (TOAPS).
1.3 The values stated in inch-pound units are to be regarded
as the standard. The values given in parentheses are for
4. Apparatus
information only.
4.1 Offıcial Test Aerosol II (OTA II)/Tentative Offıcial Aque-
1.4 This standard does not purport to address all of the
ous Pressurized Spray (TOAPS), to be dispensed from the
safety concerns, if any, associated with its use. It is the
container in which it is supplied. Ensure that the OTA II/
responsibility of the user of this standard to establish appro-
TOAPS dispenser meets the specifications designated on its
priate safety and health practices and determine the applica-
label.
bility of regulatory limitations prior to use.
4.2 Test Specimen Dispenser—No restrictions are made on
2. Terminology the dispenser. However, the test results apply only to the
particular test specimen as dispensed from the particular unit
2.1 Definitions of Terms Specific to This Standard:
employed.
2.1.1 aerosols—for this method, pressurized formulations
4.3 Cockroaches—The test insects shall be healthy, normal,
generally containing 20 % or less of low-volatile ingredients
undeformed last nymphal instars or young males of the
(insecticides, base oils, solvents, etc.) and 80 % or more of
German cockroach, (Blattella germanica L). Wild cockroaches
propellant (fluorinated hydrocarbons, liquefied petroleum
shall not be used.
gases, etc.).
4.3.1 Recently emerged last nymphal instars (for example,
2.1.2 pressurized sprays—for this method, formulations
those whose pigmentation is not dark) shall not be used for
generally containing more than 20 % low-volatile ingredients
testing purposes. It is recommended that the last nymphal-
(insecticides, base oils, solvents, etc.) and 3 to 80 % propellant
instar stage shall have been attained at least 3 days prior to
(fluorinated hydrocarbons, liquefied petroleum gases, com-
testing.
pressed gases, etc.).
4.3.2 Any suitable method permitting the production of
2.1.3 moribund—pertaining to any insect that shows signs
large numbers of test insects under controlled temperature and
of life, but is incapable of normal locomotion.
humidity conditions may be employed. The rearing technique
3. Significance and Use
described by Woodbury and Barnhart, which makes good use
of a brood chamber containing adult females from which large
3.1 This test method provides a satisfactory means of
numbers of first instar nymphs may be collected at frequent
determining the relative efficiency of aerosol formulations
intervals, has been successfully used.
when applied as direct sprays to cockroaches. It is not designed
4.3.3 All test insects shall be reared under uniform condi-
to measure the residual action.
tions.
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides and is the direct responsibility of Subcommittee E35.12 on Insect Control
Agents. It was originally developed by the Chemical Specialties Manufacturers Available from the Chemical Specialties Manufacturers Assn. 1001 Connecti-
Association. cut Ave. S.W., Washington, DC 20036.
Current edition approved Oct. 10, 1996. Published December 1996. Originally Woodbury, E. N., and Barnhart, C. S., “Tests on Crawling Insects,” Soap and
approved in 1979. Last previous edition approved in 1990 as E 654 – 90. Sanitary Chemicals, SSCHA, Vol 15, No. 9, 1939.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E654–96 (2003)
4.4 Rearing Room, of any convenient size, free of strong 5.2 Align the dispensers on the adjustable shelf and deter-
drafts and maintained at a temperature of 80 6 2°F (27 6 1°C) mine their settings to permit rapid handling during testing.
and a relative humidity of 506 5 %. This room should be
5.3 Run a minimum of ten test groups for the test specimen
separate from the testing room and should be ventilated to in conjunction with ten test groups using the OTA II/ TOAPS.
minimize odors.
Make an equal number of replicates for members of any test
4.5 Test Room, maintained at a temperature of 80 6 2°F (27 series on a given test day.
6 1°C) and a relative humidity of 50 6 5 %, of a convenient
5.4 The dosages shall be approximately the same through-
size to permit the operator to conduct the test efficiently. out a given test series and shall be of such magnitude as to
4.6 Spray Chamber, with walls of solid material, 18 in. (457
result in an average of 50 to 75 % of the insects dead and
mm) square, 25 to 30 in. (635 to 762 mm) high, with an open moribund at 24 h with the OTA II/TOAPS. Consider average
top and floor.
dosages the same if they agree within 0.2 g.
4.6.1 The floor of the chamber shall be covered with ⁄2-in.
5.5 Weigh the test dispensers both before and after the
(13-mm) mesh, wire hardware cloth, with suitable guides
spraying of each test group and record the weight of the
fastened to permit the centering of the treatment container in a
material used.
definitepositionwithrespecttotheOTA II/TOAPSnozzleand
the test specimen dispenser. The front wall may be a sliding
6. Procedure
door to permit convenient access to the chamber interior. An
6.1 Isolate the last instar nymphs in the recovery dishes in
adjustable hinged shelf shall be attached to the outside of the
groups of 20 with a suction device, by immobilizing them with
center of the back upper edge of the chamber.
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