Standard Test Method for Evaluation of First Aid Antiseptic Drug Products (Withdrawn 2003)

SCOPE
1.1 The tests described in this test method are designed to evaluate antimicrobial agents in formulations intended for use as first aid antiseptic products for their ability to reduce or suppress the growth, or both, of the skin microflora.
1.2 A knowledge of microbiological techniques is required for these procedures.
1.3 In this test method metric units are used for all applications, except for distance, in which case inches are used and metric units follow in parenthesis.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Status
Historical
Publication Date
31-Dec-1993
Withdrawal Date
19-Feb-2003
Current Stage
Ref Project

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ASTM E1589-94 - Standard Test Method for Evaluation of First Aid Antiseptic Drug Products (Withdrawn 2003)
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E1589–94
Standard Test Method for
Evaluation of First Aid Antiseptic Drug Products
This standard is issued under the fixed designation E1589; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope samples plated for total aerobic bacteria count. The counts
obtainedfromthetreatedsitesarecomparedtocountsobtained
1.1 The tests described in this test method are designed to
from untreated occluded sites.
evaluate antimicrobial agents in formulations intended for use
3.1.2 Effect of the Test Agent to Suppress the Growth of
as first aid antiseptic products for their ability to reduce or
Normal Skin Flora When Applied As a Dressing—The dress-
suppress the growth, or both, of the skin microflora.
ings are applied to the forearm for 24 h. The density of the
1.2 A knowledge of microbiological techniques is required
resident organisms that develop under the dressings are com-
for these procedures.
pared to the population that develops on a similar untreated
1.3 In this test method metric units are used for all applica-
occluded site. Following 24 h of occlusion, the sites are
tions, except for distance, in which case inches are used and
microbiologically sampled and the samples plated for total
metric units follow in parenthesis.
aerobic bacteria count.
1.4 This standard does not purport to address all of the
3.2 Theprincipalofthetestisthatthemicrofloraofforearm
safety concerns, if any, associated with its use. It is the
skin is sparse. The impermeable dressing will increase surface
responsibility of the user of this standard to establish appro-
moisture by preventing diffusional water loss and thus expand
priate safety and health practices and determine the applica-
transient resident skin microorganisms. A significant effect by
bility of regulatory limitations prior to use.
thetestagentwillbereflectedbysignificantlylowerpopulation
2. Referenced Documents
on the treated site.
2.1 ASTM Standards:
4. Significance and Use
E1054 Practices for Evaluating Inactivators of Antimicro-
4.1 The procedures in this test method should be used to
bial Agents Used in Disinfectant, Sanitizer, Antiseptic, or
evaluate in vivo the antimicrobial activity of drug products
Preserved Products
applied topically to the skin that are intended to help prevent
3. Summary of Test Methods
infection in minor cuts, scrapes and burns.
4.1.1 This test method is applicable for testing liquids,
3.1 These test methods describe standard in vivo techniques
ointments, powders, films, or dressing containing or impreg-
to determine the following:
nated with an antimicrobial for their effect to reduce an
3.1.1 Effect of the Test Agent to Reduce An Artificially
enhancedskinmicrofloraortheireffectstosuppressthegrowth
Enhanced Skin Microbial Flora—The forearms of subjects are
of the skin flora, or both.
occluded for 48 h prior to application of the test agents to
increase the microbial population on the skin of the volar
5. Apparatus
forearm surface. At treatment the occlusion material is re-
5.1 Colony Counter—Anyofseveraltypesmaybeused,for
moved and the skin is allowed to dry, the test agents are then
example, Quebec colony counter.
appliedtoselectedsites.Atapre-determinedtime(s)following
5.2 Incubator—Any incubator capable of maintaining a
application, the sites are microbiologically sampled and the
temperature of 35 6 2°C may be used.
5.3 Sterilizer—Any suitable steam sterilizer capable of
This test method is under the jurisdiction of ASTM Committee E-35 on
producing the conditions or sterilization.
PesticidesandisthedirectresponsibilityofSubcommitteeE35.15onAntimicrobial
5.4 Timer (Stop-Clock)—Onethatcanbereadforhoursand
Agents.
minutes.
Current edition approved Jan. 15, 1994. Published March 1994.
Annual Book of ASTM Standards, Vol 11.05.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E1589
6. Reagents and Materials 7.1.4 Subject Instructions—Subjects are to refrain from
usinganyproductcontaininganantimicrobialagentforatleast
6.1 Bacteriological Pipette—5.0 and 2.2 mL or 1.1 mL
3 one week prior to the test. Kits containing non-medicated bar
capacity.
soap, shampoo, and roll-on antiperspirant for use during this
6.2 Water Dilution Bottles—Anysterilizableglasscontainer
timeareprovided.Subjectsareinstructednotto usemedicated
having a 150 to 200 mL capacity and tight closure may be
creams, ointments, or take antibiotics. Swimming is not per-
used.
mitted.Subjectsareinstructednottoshowerinthe48hperiod
6.3 Scrubbing Cups—Sterile glass cylinders, height ap-
proceeding the sampling period; however, a sponge bath is
proximately 2.5 cm, inside diameter 2.54 cm.
permitted. These instructions are to be given to each subject
6.4 Rubber Policeman—Can be fashioned in the laboratory
verbally and in written form.
or purchased from most laboratory supply houses.
7.1.5 Test Site Occlusion—Following a two-week wash-out
6.5 Test Formulation—With directions for use.
period, the forearms of each of the twelve volunteers are
6.6 Polyvinyldene-chloride Wrap —Used to occlude skin
occluded with polyvinyldene-chloride wrap patches. The
sites.
polyvinyldene-chloride wrap is cut to fashion a patch that will
6.7 Sampling Solution—Dissolve 0.4 g KH PO , 10.1 g
2 4
coverthevolaraspectoftheforearm,approximately8to10cm
Na HPO and 1.0 g isoctylphenoxypolyethoxyethanol in1L
2 4
by 18 to 20 cm. A patch is placed in the mid-volar surface of
of distilled water. Include in this formulation an activator
each arm and anchored with tape.
specific for the antimicrobial in the test formulation (see
Practices E1054). Adjust to pH 7.8. Dispense in 100 mL
7.1.6 Treatment Procedure—A test product treatment and
volumes and sterilize for 20 min at 121°C. two control sites, no treatment and vehicle control (formula
6.8 Dilution Fluid—Butterfield’s phosphatebufferedwater
without the active), are located on the volar aspect of each
adjusted to pH 7.2, and containing an antimicrobial activator forearm, each site measures 3.5 cm by 3.5 cm.The assignment
specific for the test formulation. (See Practices E1054.)
of the treatment and two controls to the three sites on each
6.9 Plating Medium—Soybean-casein digest agar. forearm is made according to a predetermined randomization
scheme. Approximately 48 h following occlusion, individual
7. Procedure
sitesareexposed10minpriortotreatment,toallowtheareato
7.1 Reduction of Microbial Flora by Products That Are
...

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