ASTM D5245-19

Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses

SIGNIFICANCE AND USE
4.1 This practice provides uniform guidance for cleaning the laboratory glassware, plasticware, and equipment used in routine microbiological analyses. However, tests that are extremely sensitive to toxic agents (such as virus assays) may require more stringent cleaning practices.2
SCOPE
1.1 In microbiology, clean glassware is crucial to ensure valid results. Previously used or new glassware must be thoroughly cleaned. Laboratory ware and equipment that are not chemically clean are responsible for considerable losses in personnel time and supplies in many laboratories. These losses may occur as down time when experiments clearly have been adversely affected and as invalid data that are often attributed to experimental error. Chemical contaminants that adversely affect experimental results are not always easily detected. This practice describes the procedures for producing chemically clean glassware.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific precautions, see Section 6, 5.7.3.1, and 8.3.1.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Historical
• Publication Date: 31-Mar-2019
• ICS: 07.100.01 - Microbiology in general
• Technical Committee: D19 - Water
• Drafting Committee: D19.24 - Water Microbiology

Relations

• Replaces: ASTM D5245-92(2012) - Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses
• Effective Date: 01-Apr-2019
• Replaced By: ASTM D5245-19(2024) - Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses
• Effective Date: 01-Apr-2024
• Refers: ASTM D1129-13(2020)e2 - Standard Terminology Relating to Water
• Effective Date: 01-May-2020
• Refers: ASTM D1129-10 - Standard Terminology Relating to Water
• Effective Date: 01-Mar-2010
• Refers: ASTM D1129-06a - Standard Terminology Relating to Water
• Effective Date: 01-Sep-2006
• Refers: ASTM D1129-06ae1 - Standard Terminology Relating to Water
• Effective Date: 01-Sep-2006
• Refers: ASTM D1193-06 - Standard Specification for Reagent Water
• Effective Date: 01-Mar-2006
• Refers: ASTM D1129-06 - Standard Terminology Relating to Water
• Effective Date: 15-Feb-2006
• Refers: ASTM D1129-04 - Standard Terminology Relating to Water
• Effective Date: 01-Mar-2004
• Refers: ASTM D1129-04e1 - Standard Terminology Relating to Water
• Effective Date: 01-Mar-2004
• Refers: ASTM D1129-03a - Standard Terminology Relating to Water
• Effective Date: 10-Aug-2003
• Refers: ASTM D1129-03 - Standard Terminology Relating to Water
• Effective Date: 10-Mar-2003
• Refers: ASTM D1129-02a - Standard Terminology Relating to Water
• Effective Date: 10-Jul-2002
• Refers: ASTM D1129-01 - Standard Terminology Relating to Water
• Effective Date: 10-Jul-2002
• Refers: ASTM D1129-99a - Standard Terminology Relating to Water
• Effective Date: 10-Feb-2002

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D5245 − 19
Standard Practice for
Cleaning Laboratory Glassware, Plasticware, and Equipment
1,2
Used in Microbiological Analyses
This standard is issued under the fixed designation D5245; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope* 2. Referenced Documents
3
1.1 In microbiology, clean glassware is crucial to ensure 2.1 ASTM Standards:
valid results. Previously used or new glassware must be D1129 Terminology Relating to Water
thoroughly cleaned. Laboratory ware and equipment that are D1193 Specification for Reagent Water
not chemically clean are responsible for considerable losses in
personnel time and supplies in many laboratories. These losses 3. Terminology
may occur as down time when experiments clearly have been
3.1 Definitions:
adversely affected and as invalid data that are often attributed
3.1.1 For definitions of terms used in this standard, refer to
to experimental error. Chemical contaminants that adversely
Terminology D1129.
affect experimental results are not always easily detected. This
practice describes the procedures for producing chemically
4. Significance and Use
clean glassware.
4.1 This practice provides uniform guidance for cleaning
1.2 The values stated in SI units are to be regarded as
the laboratory glassware, plasticware, and equipment used in
standard. No other units of measurement are included in this
routine microbiological analyses. However, tests that are ex-
standard.
tremely sensitive to toxic agents (such as virus assays) may
2
1.3 This standard does not purport to address all of the require more stringent cleaning practices.
safety concerns, if any, associated with its use. It is the
5. Reagents
responsibility of the user of this standard to establish appro-
priate safety, health, and environmental practices and deter-
5.1 Purity of Reagents—Reagent grade chemicals shall be
mine the applicability of regulatory limitations prior to use.
used in all tests. Unless otherwise indicated, it is intended that
For specific precautions, see Section 6, 5.7.3.1, and 8.3.1.
all reagents conform to the specifications of the Committee on
1.4 This international standard was developed in accor-
Analytical Reagents of the American Chemical Society where
4
dance with internationally recognized principles on standard-
such specifications are available. Other grades may be used,
ization established in the Decision on Principles for the
provided it is first ascertained that the reagent is of sufficiently
Development of International Standards, Guides and Recom-
high purity to permit its use without lessening the accuracy of
mendations issued by the World Trade Organization Technical
the determination.
Barriers to Trade (TBT) Committee.
1 3
This practice is under the jurisdiction of ASTM Committee D19 on Water and For referenced ASTM standards, visit the ASTM website, www.astm.org, or
is the direct responsibility of Subcommittee D19.24 on Water Microbiology. contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
Current edition approved April 1, 2019. Published April 2019. Originally Standards volume information, refer to the standard’s Document Summary page on
approved in 1992. Last previous edition approved in 2012 as D5245 – 92 (2012). the ASTM website.
4
DOI: 10.1520/D5245-19. Reagent Chemicals, American Chemical Society Specifications, American
2
A significant portion of this practice was taken from: Berg, G., Safferman, R. Chemical Society, Washington, DC. For suggestions on the testing of reagents not
S., Dahling, D. R., Berman, D., and Hurst, C. J., USEPA Manual of Methods for listed by the American Chemical Society, see Analar Standards for Laboratory
Virology, EPA-600/4-84-013, Chapter 2, “Cleansing Laboratory Ware and Chemicals, BDH Ltd., Poole, Dorset, U.K., and the United States Pharmacopeia
Equipment, Environmental Monitoring and Support Laboratory—Cincinnati,” and National Formulary, U.S. Pharmacopeial Convention, Inc. (USPC), Rockville,
USEPA, Cincinnati, OH. MD.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D5245 − 19
5.2 Purity of Water—Unless otherwise indicated, references 6.2 Sterilize contaminated laboratory ware and equipment
to water shall be understood to mean Type IV of Specificatio
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: D5245 − 92 (Reapproved 2012) D5245 − 19
Standard Practice for
Cleaning Laboratory Glassware, Plasticware, and Equipment
1,2
Used in Microbiological Analyses
This standard is issued under the fixed designation D5245; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope Scope*
1.1 In microbiology, clean glassware is crucial to ensure valid results. Previously used or new glassware must be thoroughly
cleaned. Laboratory ware and equipment that are not chemically clean are responsible for considerable losses in personnel time
and supplies in many laboratories. These losses may occur as down time when experiments clearly have been adversely affected
and as invalid data that are often attributed to experimental error. Chemical contaminants that adversely affect experimental results
are not always easily detected. This practice describes the procedures for producing chemically clean glassware.
1.2 The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of
regulatory limitations prior to use. For specific precautions, see Section 56, 7.3.15.7.3.1, and Note 18.3.1and Note 2.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
3
2.1 ASTM Standards:
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
3. Terminology
3.1 Definitions:
3.1.1 For definitions of terms used in this standard, refer to Terminology D1129.
4. Significance and Use
4.1 This practice provides uniform guidance for cleaning the laboratory glassware, plasticware, and equipment used in routine
microbiological analyses. However, tests that are extremely sensitive to toxic agents (such as virus assays) may require more
2
stringent cleaning practices. practices.
5. Reagents
5.1 Purity of Reagents—Reagent grade chemicals shall be used in all tests. Unless otherwise indicated, it is intended that all
reagents conform to the specifications of the Committee on Analytical Reagents of the American Chemical Society where such
1
This practice is under the jurisdiction of ASTM Committee D19 on Water and is the direct responsibility of Subcommittee D19.24 on Water Microbiology.
Current edition approved June 1, 2012April 1, 2019. Published August 2012April 2019. Originally approved in 1992. Last previous edition approved in 20052012 as
D5245 – 92 (2005).(2012). DOI: 10.1520/D5245-92R12.10.1520/D5245-19.
2
A significant portion of this practice was taken from: Berg, G., Safferman, R. S., Dahling, D. R., Berman, D., and Hurst, C. J., USEPA Manual of Methods for Virology,
EPA-600/4-84-013, Chapt. 2, “Cleansing Laboratory Ware and Equipment, Environmental Monitoring and Support Laboratory—Cincinnati,” USEPA, Cincinnati, OH.A
significant portion of this practice was taken from: Berg, G., Safferman, R. S., Dahling, D. R., Berman, D., and Hurst, C. J., USEPA Manual of Methods for Virology,
EPA-600/4-84-013, Chapter 2, “Cleansing Laboratory Ware and Equipment, Environmental Monitoring and Support Laboratory—Cincinnati,” USEPA, Cincinnati, OH.
3
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D5245 − 19
4
specifications are available. available. Other grades may be used, provided it is first ascertained that the reagent is of sufficiently
high purity to permit its use without lessening the accuracy of the determination
...

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D5245 − 19
Standard Practice for
Cleaning Laboratory Glassware, Plasticware, and Equipment
1,2
Used in Microbiological Analyses
This standard is issued under the fixed designation D5245; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope* 2. Referenced Documents
3
1.1 In microbiology, clean glassware is crucial to ensure 2.1 ASTM Standards:
valid results. Previously used or new glassware must be D1129 Terminology Relating to Water
thoroughly cleaned. Laboratory ware and equipment that are D1193 Specification for Reagent Water
not chemically clean are responsible for considerable losses in
3. Terminology
personnel time and supplies in many laboratories. These losses
may occur as down time when experiments clearly have been
3.1 Definitions:
adversely affected and as invalid data that are often attributed
3.1.1 For definitions of terms used in this standard, refer to
to experimental error. Chemical contaminants that adversely
Terminology D1129.
affect experimental results are not always easily detected. This
practice describes the procedures for producing chemically
4. Significance and Use
clean glassware.
4.1 This practice provides uniform guidance for cleaning
1.2 The values stated in SI units are to be regarded as
the laboratory glassware, plasticware, and equipment used in
standard. No other units of measurement are included in this
routine microbiological analyses. However, tests that are ex-
standard.
tremely sensitive to toxic agents (such as virus assays) may
2
require more stringent cleaning practices.
1.3 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
5. Reagents
responsibility of the user of this standard to establish appro-
priate safety, health, and environmental practices and deter-
5.1 Purity of Reagents—Reagent grade chemicals shall be
mine the applicability of regulatory limitations prior to use.
used in all tests. Unless otherwise indicated, it is intended that
For specific precautions, see Section 6, 5.7.3.1, and 8.3.1.
all reagents conform to the specifications of the Committee on
1.4 This international standard was developed in accor-
Analytical Reagents of the American Chemical Society where
4
dance with internationally recognized principles on standard-
such specifications are available. Other grades may be used,
ization established in the Decision on Principles for the
provided it is first ascertained that the reagent is of sufficiently
Development of International Standards, Guides and Recom-
high purity to permit its use without lessening the accuracy of
mendations issued by the World Trade Organization Technical
the determination.
Barriers to Trade (TBT) Committee.
1 3
This practice is under the jurisdiction of ASTM Committee D19 on Water and For referenced ASTM standards, visit the ASTM website, www.astm.org, or
is the direct responsibility of Subcommittee D19.24 on Water Microbiology. contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
Current edition approved April 1, 2019. Published April 2019. Originally Standards volume information, refer to the standard’s Document Summary page on
approved in 1992. Last previous edition approved in 2012 as D5245 – 92 (2012). the ASTM website.
4
DOI: 10.1520/D5245-19. Reagent Chemicals, American Chemical Society Specifications, American
2
A significant portion of this practice was taken from: Berg, G., Safferman, R. Chemical Society, Washington, DC. For suggestions on the testing of reagents not
S., Dahling, D. R., Berman, D., and Hurst, C. J., USEPA Manual of Methods for listed by the American Chemical Society, see Analar Standards for Laboratory
Virology, EPA-600/4-84-013, Chapter 2, “Cleansing Laboratory Ware and Chemicals, BDH Ltd., Poole, Dorset, U.K., and the United States Pharmacopeia
Equipment, Environmental Monitoring and Support Laboratory—Cincinnati,” and National Formulary, U.S. Pharmacopeial Convention, Inc. (USPC), Rockville,
USEPA, Cincinnati, OH. MD.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D5245 − 19
5.2 Purity of Water—Unless otherwise indicated, references 6.2 Sterilize contaminated laboratory ware and equipment
to water shall be understood to mean Type IV of Specification before cleaning.
D1193.
6.3 Transport hazardous acids only in appropriate safety
5.3 Bromothymol blue, 0.4 %.
carriers.
5.3.1 Bromothymol blue.
...