ASTM E734-80(2005)

Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)

ABSTRACT
This specification describes the physical requirements and corresponding test methods for disposable glass blood sample capillary tubes for use in microhematocrit procedures. Covered here are two different types of capillary tubes, namely, Type I (coated with heparin), and Type II (uncoated). The tubes shall be fabricated from Type I, Class B borosilicate glass, or Type II soda lime glass. Conversely, the heparin used for coating Type I tubes shall be of ammonium salt isolated from the lungs or intestinal mucosa of beef or pork origin. The tubes shall conform to specified requirements for design, dimension, workmanship, color coding, and lot or control number. They should also pass the following tests for capillarity, fluidity, sheep plasma, positive and negative controls, human whole blood, heparin potency assay, and resistance to centrifugal force.
SCOPE
1.1 This specification covers disposable glass blood sample capillary tubes for use in microhematocrit procedures.

General Information

• Status: Historical
• Publication Date: 30-Sep-2005
• Technical Committee: E41 - Laboratory Apparatus
• Drafting Committee: E41.01 - Laboratory Ware and Supplies

Relations

• Replaces: ASTM E734-80(1999)e1 - Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)
• Effective Date: 01-Oct-2005
• Replaced By: ASTM E734-80(2010) - Standard Specification for Disposable Glass Blood Sample Capillary Tube (Microhematocrit)
• Effective Date: 01-Jul-2010

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E734 – 80 (Reapproved 2005)
Standard Specification for
Disposable Glass Blood Sample Capillary Tube
(Microhematocrit)
This standard is issued under the fixed designation E734; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 5.2 Heparin—Heparin shall be of ammonium salt isolated
from the lungs or intestinal mucosa of beef or pork origin.The
1.1 This specification covers disposable glass blood sample
heparin potency shall be 1 mg of ammonium heparin com-
capillary tubes for use in microhematocrit procedures.
pound which shall be equal to at least 60 United States
2. Referenced Documents
Pharmacopeia (USP) units. Dry or wet heparin may be applied
to the tube.
2.1 ASTM Standards:
E438 Specification for Glasses in Laboratory Apparatus
6. Physical Requirements Physical Requirements
2.2 Other Standard:
6.1 Design—The capillary tubes shall be straight and open
USP XIX United States Pharmacopeia
atbothendswithoutliporconstriction.Thecapillarytubemay
3. Terminology belightlyfirepolishedononeend.Theboreofthetubeshallbe
uniform and not vary in excess of 0.025 mm in 75 mm.
3.1 Definitions of Terms Specific to This Standard:
6.2 Dimensions—Type I and Type II capillary tubes shall
3.1.1 disposable capillary tubes—in accordance with this
have a length of 75 6 0.5 mm. Inside diameter shall be from
specification and the expected product performance expressed
1.07 to 1.24 mm. Wall thickness shall be 0.20+0.03,−0.02
in this standard, those capillary tubes which are to be used one
mm, as specified in Fig. 1.
time only. Any institution or individual who reuses a dispos-
6.3 Workmanship—The capillary tubes shall be free of
able capillary tube must bear full responsibility for its safety
defects that noticeably detract from their appearance or impair
and effectiveness.
their serviceability. The capillary tube shall be free of lint, or
4. Classification
significant foreign matter, loose or embedded, when viewed
under normal room lighting. The tube ends shall be cut
4.1 This specification covers two different disposable glass
approximately 90° to the tube axis and shall not be cracked or
sample capillary tubes as follows:
have jagged ends or chips that enter the bore of the tubing.
Type I—Coated with heparin.
6.4 Color Coding—Eachcapillarytubeshallbecolorcoded
Type II—Uncoated.
to identify the tube as coated with heparin or uncoated.Type I,
5. Materials
heparin coated, shall have a red band and Type II, uncoated,
shall have a blue band. The location of the red or blue band
5.1 Glass—The pipets made to this specification shall be
shall be as specified in Fig. 1.
fabricated from borosilicate glass, Type I, Class B, or soda-
6.5 Capillarity—The capillary tube shall be capable of
lime glass, Type II, in accordance with Specification E438.
drawing sheep plasma or human whole blood to a level within
20 mm from the far end of the tube when tested as specified in
This specification is under the jurisdiction of ASTM Committee E41 on 7.1.
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
6.6 Fluidity (Type I, Heparinized, only)—Coagulationofthe
Apparatus.
sheepplasmaorhumanwholebloodshallnotbeevidentwhen
Current edition approved May 1, 2005. Published October 2005. Originally
e1
viewed under normal room lighting and tested as specified in
approved in 1980. Last previous edition approved in 1999 as E734–80 (1999) .
DOI: 10.1520/E0734-80R05.
7.2.
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
6.7 Lot or Control Number—A lot or control number shall
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
be indicated on the capillary tube unit container and on the
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. intermediate package of containers.This lot or control number
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
E734 – 80 (2005)
FIG. 1 Type I and Type II Capillary Tubes
shall be traceable to the origin (raw material purchases) of the 7. Test Methods
manufacturing record.
7.1 Capillary Test—Testsamplecapillarytubesforcapillar-
6.8 Resistance to Centrifugal Force— Resistance to capil-
ity when held at a horizontal level. The tube shall fill with
lary tube centrifugal force sha
...