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ASTM D3862-13

(Test Method)

Standard Test Method for Retention Characteristics of 0.2-µm Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality

Standard Test Method for Retention Characteristics of 0.2-&#181m Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality

SIGNIFICANCE AND USE
5.1 Microbiological water testing procedures using membrane filtration are based on the premise that all bacteria within a specific size range will be retained by the membrane filter used. If the membrane filter does not retain these bacteria, false negative results or lowered density estimates may occur that could have serious repercussions due to the presence of unrecognized potential health hazards in the water being tested, especially in drinking water.  
5.1.1 This procedure as devised will enable the user to test each membrane filter lot number for its ability to retain all bacterial equal to, or larger than, the stated membrane pore size.  
5.2 Since this membrane is often used to sterilize nonautoclavable liquids, it is essential that the retention characteristics of this membrane are stable.
SCOPE
1.1 This test method covers a procedure to test membrane filters for their ability to retain bacteria whose diameter is equal to or slightly larger than the 0.2-µm pore size of the membrane filter.  
1.2 The procedures described are for the use of user laboratories as differentiated from manufacturers' laboratories.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

Status
Historical
Publication Date
14-Feb-2013
ICS
07.100.20 - Microbiology of water
Technical Committee
D19 - Water
Drafting Committee
D19.08 - Membranes and Ion Exchange Materials
Current Stage
Ref Project

Relations

Replaces
ASTM D3862-80(2001) - Standard Test Method for Retention Characteristics of 0.2-&#181m Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality (Withdrawn 2010)
Effective Date
15-Feb-2013
Referred By
ASTM F1671/F1671M-22 - Standard Test Method for Resistance of Materials Used in Protective Clothing to Penetration by Blood-Borne Pathogens Using Phi-X174 Bacteriophage Penetration as a Test System
Effective Date
15-Feb-2013

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ASTM D3862-13 - Standard Test Method for Retention Characteristics of 0.2-&#181m Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water QualityASTM D3862-13 - Standard Test Method for Retention Characteristics of 0.2-&#181m Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality
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ASTM D3862-13 - Standard Test Method for Retention Characteristics of 0.2-&#181m Membrane Filters Used in Routine Filtration Procedures for the Evaluation of Microbiological Water Quality
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NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D3862 − 13
Standard Test Method for
Retention Characteristics of 0.2-µm Membrane Filters Used
in Routine Filtration Procedures for the Evaluation of
1
Microbiological Water Quality
This standard is issued under the fixed designation D3862; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope with one of the rosaniline dyes such as crystal violet, methyl
violet, or gentian violet and treating with iodine. Those that
1.1 This test method covers a procedure to test membrane
resist decolorization remain blue or violet and are designated
filtersfortheirabilitytoretainbacteriawhosediameterisequal
Gram-positive; those that are decolorized and take up the red
to or slightly larger than the 0.2-µm pore size of the membrane
counterstain, such as neutral red, safranin, or dilute carbol
filter.
fuchsin are termed Gram-negative.
1.2 The procedures described are for the use of user
3.2.2 vacuum, n—for the procedure used, source of suction
laboratories as differentiated from manufacturers’ laboratories.
that can produce a reading of 500 to 600 mm Hg on a vacuum
1.3 The values stated in SI units are to be regarded as
gage.
standard. No other units of measurement are included in this
standard.
4. Summary of Test Method
1.4 This standard does not purport to address all of the
4.1 This test method is based on the cultivation of organ-
safety concerns, if any, associated with its use. It is the
isms whose diameters are equal to or slightly larger than pores
responsibility of the user of this standard to establish appro-
of the membrane filter to be tested and then filtering a specific
priate safety and health practices and determine the applica-
aliquot containing organisms through the membrane followed
bility of regulatory limitations prior to use.
by an examination of the filtrate after incubation for sterility.A
sterile filtrate indicates complete retention of the organism and
2. Referenced Documents
validates the ability of the membrane to retain bacteria equal to
2
or slightly larger than the stated pore size.
2.1 ASTM Standards:
D1129 Terminology Relating to Water
5. Significance and Use
D1193 Specification for Reagent Water
5.1 Microbiological water testing procedures using mem-
3. Terminology
brane filtration are based on the premise that all bacteria within
a specific size range will be retained by the membrane filter
3.1 Definitions:
used.Ifthemembranefilterdoesnotretainthesebacteria,false
3.1.1 For definitions of terms used in this test method, refer
negative results or lowered density estimates may occur that
to Terminology D1129.
could have serious repercussions due to the presence of
3.2 Definitions of Terms Specific to This Standard:
unrecognizedpotentialhealthhazardsinthewaterbeingtested,
3.2.1 Gram’s stain, n—a routine bacterial stain that divides
especially in drinking water.
bacteria into two categories, depending on whether they can be
5.1.1 This procedure as devised will enable the user to test
decolorized with acetone, alcohol, or aniline oil after staining
each membrane filter lot number for its ability to retain all
bacterial equal to, or larger than, the stated membrane pore
1
This test method is under the jurisdiction of ASTM Committee D19 on Water size.
and is the direct responsibility of Subcommittee D19.08 on Membranes and Ion
5.2 Since this membrane is often used to sterilize nonauto-
Exchange Materials.
clavable liquids, it is essential that the retention characteristics
Current edition approved Feb. 15, 2013. Published July 2013. Originally
approved in 1990. Last previous edition approved in 2011 as D3862 – 80 (2001),
of this membrane are stable.
which was withdrawn July 2010 and reinstated in February 2013. DOI: 10.1520/
D3862-13.
6. Apparatus
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
6.1 Membrane Filtration Units, six.
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website. 6.2 Vacuum Source, with trap vessel.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
D3862 − 13
6.3 Filtering flasks, 1-L, with vacuum tubing into which a sufficiently high purity to permit its use without lessening the
glasstubeandaY-tubehavebeenincorporatedasinFig.1.The accuracy of the determination.
free end of the Y-tube is connected by tubing to a sterile
7.2 Purity of Water—Unless otherwise indicated, reference
bacterial air vent.The tubing
...

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Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method

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5.1 Sulfate reducing archaea and bacteria are known to contribute to microbiologically influenced corrosion.  
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5.3 Traditional, culture-dependent methods such as those described in Test Methods D4412, prescribe incubation periods of as long as 21 days before assigning a below detection limit (BDL) score to a specimen. Moreover, it is well known that not all SRP will proliferate in the nutrient media specified in Test Methods D4412.  
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SCOPE
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