ASTM E2783-22

Standard Test Method for Assessment of Antimicrobial Activity for Water Miscible Compounds Using a Time-Kill Procedure

SIGNIFICANCE AND USE
5.1 This procedure may be used to assess the in vitro reduction of a microbial population of test organisms after exposure to a test material.
SCOPE
1.1 This test method measures the changes of a population of aerobic and anaerobic microorganisms within a specific sampling time when tested against antimicrobial test materials in vitro. The organisms used are standardized as to growth requirements and inoculum preparation and must grow under the conditions of the test. The primary purpose of this test method is to provide a set of standardized conditions and test organisms to facilitate comparative assessments of antimicrobial materials miscible in aqueous systems.  
1.2 This test method allows the option of using a test sample size of 10 mL or 100 mL.  
1.3 Knowledge of microbiological techniques is required for this procedure.  
1.4 Aseptic technique should be practiced at all times.  
1.5 In this test method, SI units are used for all applications, except for distance in which case inches are used and SI units follow in parentheses.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

• Status: Published
• Publication Date: 31-Mar-2022
• ICS: 07.100.20 - Microbiology of water
• Technical Committee: E35 - Pesticides, Antimicrobials, and Alternative Control Agents
• Drafting Committee: E35.15 - Antimicrobial Agents

Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E2783 − 22
Standard Test Method for
Assessment of Antimicrobial Activity for Water Miscible
1
Compounds Using a Time-Kill Procedure
This standard is issued under the fixed designation E2783; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
2
2.1 ASTM Standards:
1.1 This test method measures the changes of a population
E691Practice for Conducting an Interlaboratory Study to
of aerobic and anaerobic microorganisms within a specific
Determine the Precision of a Test Method
sampling time when tested against antimicrobial test materials
E1054PracticesforEvaluationofInactivatorsofAntimicro-
in vitro. The organisms used are standardized as to growth
bial Agents
requirements and inoculum preparation and must grow under
the conditions of the test. The primary purpose of this test
3. Terminology
method is to provide a set of standardized conditions and test
organisms to facilitate comparative assessments of antimicro-
3.1 Definitions:
bial materials miscible in aqueous systems.
3.1.1 antimicrobial, adj—describes an agent that kills or
inactivates microorganisms or suppresses their growth or
1.2 Thistestmethodallowstheoptionofusingatestsample
reproduction.
size of 10 mL or 100 mL.
3.1.2 drug, n—articles intended for use in the diagnosis,
1.3 Knowledge of microbiological techniques is required
cure, mitigation, treatment, or prevention of disease in man or
for this procedure.
other animals. Drugs are intended to affect the structure or any
function of the body of man or other animals.
1.4 Aseptic technique should be practiced at all times.
3.1.3 initial microbial population, n—bacterial count (CFU/
1.5 Inthistestmethod,SIunitsareusedforallapplications,
mL) in the final volume of test material.Also known as initial
except for distance in which case inches are used and SI units
bacterial population, numbers control or control.
follow in parentheses.
3.1.4 inoculum, n—in microbiology, a specimen compro-
1.6 This standard does not purport to address all of the
mised of living spores, bacteria, yeast or the multicellular
safety concerns, if any, associated with its use. It is the
filamentous fungi, or combination of two or more types of
responsibility of the user of this standard to establish appro-
microorganisms,thatareintroducedintoatestmediumoronto
priate safety, health, and environmental practices and deter-
aspecimentobetestedinordertoinvestigatetheabilityofthe
mine the applicability of regulatory limitations prior to use.
medium or specimen to support microbial growth or to
1.7 This international standard was developed in accor-
investigate its antimicrobial properties.
dance with internationally recognized principles on standard-
3.1.5 microbiocide, n—a physical or chemical agent that
ization established in the Decision on Principles for the
kills microorganisms.
Development of International Standards, Guides and Recom-
3.1.6 neutralization, n—the process for inactivating or
mendations issued by the World Trade Organization Technical
quenching the activity of a microbiocide, often achieved
Barriers to Trade (TBT) Committee.
through physical (for example, filtration or dilution) or chemi-
cal means.
1
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
2
responsibility of Subcommittee E35.15 on Antimicrobial Agents. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Current edition approved April 1, 2022. Published June 2022. Originally contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
approved in 2010. Last previous edition approved in 2016 as E2783–11(2016). Standards volume information, refer to the standard’s Document Summary page on
DOI: 10.1520/E2783–22. the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2783 − 22
3.1.7 room temperature, n—temperature in the range of 6.14 Vortex Mixer—Any suitable vortex mixer capable of
20°C to 30°C (68°F to 85°F). mixing test material and diluents.
3.1.8 test material, n—a formulation which incorporates
6.15 Waterbath—Any waterbath capable of maintaining
antimicrobial ingredient(s). Also known as test formulation.
suitable temperature.
3.1.9 total test volume, n—the volume of test material plus
the
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E2783 − 11 (Reapproved 2016) E2783 − 22
Standard Test Method for
Assessment of Antimicrobial Activity for Water Miscible
1
Compounds Using a Time-Kill Procedure
This standard is issued under the fixed designation E2783; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method measures the changes of a population of aerobic and anaerobic microorganisms within a specific sampling
time when tested against antimicrobial test materials in vitro. The organisms used are standardized as to growth requirements and
inoculum preparation and must grow under the conditions of the test. The primary purpose of this test method is to provide a set
of standardized conditions and test organisms to facilitate comparative assessments of antimicrobial materials miscible in aqueous
systems.
1.2 This test method allows the option of using a test sample size of 10 mL or 100 mL.
1.3 Knowledge of microbiological techniques is required for this procedure.
1.4 Aseptic technique should be practiced at all times.
1.5 In this test method, SI units are used for all applications, except for distance in which case inches are used and SI units follow
in parentheses.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and determine the
applicability of regulatory limitations prior to use.
1.7 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
E1054 Practices for Evaluation of Inactivators of Antimicrobial Agents
3. Terminology
3.1 Definitions:
1
This test method is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved April 1, 2016April 1, 2022. Published May 2016June 2022. Originally approved in 2010. Last previous edition approved in 20112016 as
E2783 – 11.E2783 – 11(2016). DOI: 10.1520/E2783–11R16.10.1520/E2783–22.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2783 − 22
3.1.1 antimicrobial, n—adj—describes an agent that kills or inactivates microorganisms or suppresses their growth or
reproduction.
3.1.2 drug, n—articles intended for use in the diagnosis, cure, mitigation, treatment, or prevention of disease in man or other
animals. Drugs are intended to affect the structure or any function of the body of man or other animals.
3.1.3 initial microbial population, n—bacterial count (CFU/mL) in the final volume of test material. Also known as initial bacterial
population, numbers control or control.
3.1.4 inoculum, n—the viable microorganisms used to contaminate a sample, device or surface, often expressed as to number and
type.in microbiology, a specimen compromised of living spores, bacteria, yeast or the multicellular filamentous fungi, or
combination of two or more types of microorganisms, that are introduced into a test medium or onto a specimen to be tested in
order to investigate the ability of the medium or specimen to support microbial growth or to investigate its antimicrobial properties.
3.1.5 microbiocide, n—a physical or chemical agent that kills microorganisms.
3.1.6 neutralization, n—the process for inactivating or quenching the activity of a microbiocide. Oftenmicrobiocide, often
achieved through chemical or physical means (for example, filtration or dilution).dilution) or chemical means.
3.1.7 room
...