ASTM E2180-07
(Test Method)Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials
Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials
SCOPE
1.1 This test method is designed to evaluate (quantitatively) the antimicrobial effectiveness of agents incorporated or bound into or onto mainly flat (two dimensional) hydrophobic or polymeric surfaces. The method focuses primarily on assessing antibacterial activity; however, other microorganisms such as yeast and fungal conidia may be tested using this method.
1.2 The vehicle for the inoculum is an agar slurry which reduces the surface tension of the saline inoculum carrier and allows formation of a "pseudo-biofilm," providing more even contact of the inoculum with the test surface.Note 1
This test method facilitates the testing of hydrophobic surfaces by utilizing cells held in an agar slurry matrix. This test method, as written, is inappropriate to determine efficacy against biofilm cells, which are different both genetically and metabolically than planktonic cells used in this test.
1.3 This method can confirm the presence of antimicrobial activity in plastics or hydrophobic surfaces and allows determination of quantitative differences in antimicrobial activity between untreated plastics or polymers and those with bound or incorporated low water-soluble antimicrobial agents. Comparisons between the numbers of survivors on preservative-treated and control hydrophobic surfaces may also be made.
1.4 The procedure also permits determination of "shelf-life" or long term durability of an antimicrobial treatment which may be achieved through testing both non-washed and washed samples over a time span.
1.5 Knowledge of microbiological techniques is required for these procedures.
This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
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Designation:E2180–07
Standard Test Method for
Determining the Activity of Incorporated Antimicrobial
1
Agent(s) In Polymeric or Hydrophobic Materials
This standard is issued under the fixed designation E2180; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Polymeric materials such as vinyl pool liners, shower curtains, and various medical devices are
treatedfrequentlywithincorporatedorboundantimicrobialagents.PracticesG21isusedtodetermine
the ability of polymer materials to resist microbial attack or staining (see also Practice E1428);
2
however, none of the methods permit quantitative evaluations of incorporated antimicrobial activity.
These antimicrobials typically require contact with the microbial cell for maximal activity. When
aqueous based bacterial inoculum suspensions are applied onto a preservative-treated plastic or other
hydrophobic material, the surface tension of the polymer often causes the inocula suspension to dome.
Bacteria within the drops of inoculum may not contact the treated surface if the challenged surface
does not dry, or upon drying, cells may become layered. This test standard involves an agar slurry
inoculum vehicle that provides a relatively uniform contact of the inocula with antimicrobial-treated
hydrophobic surfaces.
1. Scope or incorporated low water-soluble antimicrobial agents. Com-
parisons between the numbers of survivors on preservative-
1.1 This test method is designed to evaluate (quantitatively)
treated and control hydrophobic surfaces may also be made.
the antimicrobial effectiveness of agents incorporated or bound
1.4 The procedure also permits determination of “shelf-life”
into or onto mainly flat (two dimensional) hydrophobic or
or long term durability of an antimicrobial treatment which
polymericsurfaces.Themethodfocusesprimarilyonassessing
may be achieved through testing both non-washed and washed
antibacterial activity; however, other microorganisms such as
samples over a time span.
yeast and fungal conidia may be tested using this method.
1.5 Knowledge of microbiological techniques is required
1.2 The vehicle for the inoculum is an agar slurry which
for these procedures.
reduces the surface tension of the saline inoculum carrier and
1.6 This standard does not purport to address all of the
allows formation of a “pseudo-biofilm,” providing more even
safety concerns, if any, associated with its use. It is the
contact of the inoculum with the test surface.
responsibility of the user of this standard to establish appro-
NOTE 1—This test method facilitates the testing of hydrophobic sur-
priate safety and health practices and determine the applica-
faces by utilizing cells held in an agar slurry matrix. This test method, as
bility of regulatory limitations prior to use.
written, is inappropriate to determine efficacy against biofilm cells, which
are different both genetically and metabolically than planktonic cells used
2. Referenced Documents
in this test.
3
2.1 ASTM Standards:
1.3 This method can confirm the presence of antimicrobial
E1054 Test Methods for Evaluation of Inactivators of An-
activity in plastics or hydrophobic surfaces and allows deter-
timicrobial Agents
mination of quantitative differences in antimicrobial activity
E1428 Test Method for Evaluating the Performance of
between untreated plastics or polymers and those with bound
Antimicrobials in or on Polymeric SolidsAgainst Staining
by Streptoverticillium reticulum (A Pink Stain Organism)
G21 Practice for Determining Resistance of Synthetic Poly-
1
This test method is under the jurisdiction of ASTM Committee E35 on
meric Materials to Fungi
Pesticides and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Nov. 1, 2007. Published November 2007. Originally
approved in 2001. Last previous edition approved in 2001 as E2180 – 01. DOI:
3
10.1520/E2180-07. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
2
Price, D.L., A.D. Sawant, and D.G. Ahearn. 1991. Assessment of the antimi- contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
crobial activity of an insoluble quaternary amine complex in plastics. J. Industr. Standards volume information, refer to the standard’s Document Summary page on
Microbiol. Vol 8 (No.2):83-89. the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1
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E2180–07
3. Terminology 6.13 Vortex Mixer.
6.14 pH
...
This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E2180–01 Designation: E 2180 – 07
Standard Test Method for
Determining the Activity of Incorporated Antimicrobial
1
Agent(s) In Polymeric or Hydrophobic Materials
This standard is issued under the fixed designation E 2180; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Polymeric materials such as vinyl pool liners, shower curtains, and various medical devices are
treated frequently with incorporated or bound antimicrobial agents. Practices G 21 and G22 areis used
to determine the ability of polymer materials to resist microbial attack or staining (see also Practice
E1428); however, none of the methods permit quantitative evaluations of incorporated antimicrobial
2
activity. These antimicrobials typically require contact with the microbial cell for maximal activity.
When aqueous based bacterial inoculum suspensions are applied onto a preservative-treated plastic or
other hydrophobic material, the surface tension of the polymer often causes the inocula suspension to
dome. Bacteria within the drops of inoculum may not contact the treated surface if the challenged
surface does not dry, or upon drying, cells may become layered. This test standard involves an agar
slurry inoculum vehicle that provides a relatively uniform contact of the inocula with antimicrobial-
treated hydrophobic surfaces.
1. Scope
1.1 Thistestmethodisdesignedtoevaluate(quantitatively)theantimicrobialeffectivenessofagentsincorporatedorboundinto
or onto mainly flat (two dimensional) hydrophobic or polymeric surfaces.The method focuses primarily on assessing antibacterial
activity; however, other microorganisms such as yeast and fungal conidia may be tested using this method.
1.2 The vehicle for the inoculum is an agar slurry which reduces the surface tension of the saline inoculum carrier and allows
formation of a “pseudo-biofilm,” providing more even contact of the inoculum with the test surface.
NOTE 1—This test method facilitates the testing of hydrophobic surfaces by utilizing cells held in an agar slurry matrix. This test method, as written,
is inappropriate to determine efficacy against biofilm cells, which are different both genetically and metabolically than planktonic cells used in this test.
1.3 Thismethodcanconfirmthepresenceofantimicrobialactivityinplasticsorhydrophobicsurfacesandallowsdetermination
of quantitative differences in antimicrobial activity between untreated plastics or polymers and those with bound or incorporated
low water-soluble antimicrobial agents. Comparisons between the numbers of survivors on preservative-treated and control
hydrophobic surfaces may also be made.
1.4 Theprocedurealsopermitsdeterminationof“shelf-life”orlongtermstabilitydurabilityofanantimicrobialtreatmentwhich
may be achieved through testing both non-washed and washed samples over a time span.
1.5 Knowledge of microbiological techniques is required for these procedures.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
3
2.1 ASTM Standards:
E 1054Practice for Evaluation of Inactivators of Antimicrobial Agents used in Disinfectant, Sanitizer, Antiseptic, or Preserved
1
This test method is under the jurisdiction ofASTM Committee E35 on Pesticides andAlternative ControlAgents and is the direct responsibility of Subcommittee E35.15
on Antimicrobial Agents.
Current edition approved Dec. 10, 2001. Published February 2002.
Current edition approved Nov. 1, 2007. Published November 2007. Originally approved in 2001. Last previous edition approved in 2001 as E 2180 – 01.
2
Price, D.L., A.D. Sawant, and D.G. Ahearn. 1991. Assessment of the antimicrobial activity of an insoluble quaternary amine complex in plastics. J. Industr. Microbiol.
Vol 8 (No.2):83-89.
3
For referencedASTM standards, visit theASTM website, www.astm.org, or contactASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, Uni
...
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