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ASTM E3371-22

(Test Method)

Standard Test Method for Measuring the Ability of a Synthetic Polymeric Material to Resist Bacterial Adherence

Standard Test Method for Measuring the Ability of a Synthetic Polymeric Material to Resist Bacterial Adherence

SIGNIFICANCE AND USE
5.1 This method can be used to evaluate the effectiveness of incorporated or bound anti-adherent agents in synthetic polymeric materials and polymeric coatings intended to reduce the attachment of bacteria to the substrate surface.  
5.2 The synthetic polymeric substrate surface may be tested repeatedly over time for assessment of persistent ability of a material to resist bacterial adherence.  
5.3 This method is to quantify the degree of bacteria colonization of a surface to assess a materials ability to resist bacterial adherence because biofilm formation can contribute to material degradation and malfunction.
SCOPE
1.1 This method is designed to evaluate (quantitatively) the number of bacteria attached to the flat, two-dimensional surfaces of synthetic polymeric materials and polymeric coatings on various substrates that may or may not contain bound or incorporated anti-adherent agents. The method focuses on assessing the ability of the surface to reduce bacterial attachment. Other microorganisms such as yeast and fungal conidia may be tested using this method.  
1.2 This test method quantitatively determines the differences in bacterial adherence seen between synthetic polymeric surfaces that allow bacterial adherence and those that do not, comparing the number of organisms recovered from the control surface to the number recovered from the test specimen surface after the contact time. Knowledge of microbiological techniques is required for these procedures.  
1.3 This test method specifies proper methods for measuring the ability of a synthetic polymeric material to resist adherence against specified organism. Due to individual sensitivities, the result of one test organism might not be applicable for other organisms.  
1.4 This test method is designed to measure the potential ability to resist bacterial adherence of a non-porous surface compared directly to a polyester control panel known to support bacterial adherence under specific testing conditions.  
1.5 Antimicrobial treated non-porous surfaces may demonstrate ability to resist bacterial adherence in this method. This method does not purport to differentiate between anti-adherence and antimicrobial activity nor is it designed to reflect specific end-use or environmental conditions. Any product that demonstrates ability to resist bacterial adherence in this method should be measured for antimicrobial activity using a separate test technique such as Test Method E2180 or ISO 22196.  
1.6 The method focuses on assessing the ability of synthetic polymeric materials and polymeric coatings on various substrates to reduce bacterial attachment. The specimen with absorbing or adhesive surfaces may be unable to be disinfected properly before testing, or may trap inoculated organism during recovery process and thus lead to a false result. This method does not apply to specimens with absorbent or adhesive surfaces.  
1.7 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard.  
1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

General Information

Status
Published
Publication Date
30-Sep-2022
ICS
07.100.99 - Other standards related to microbiology
83.080.01 - Plastics in general
Technical Committee
E35 - Pesticides, Antimicrobials, and Alternative Control Agents
Drafting Committee
E35.15 - Antimicrobial Agents
Current Stage
Ref Project

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ASTM E3371-22 - Standard Test Method for Measuring the Ability of a Synthetic Polymeric Material to Resist Bacterial AdherenceASTM E3371-22 - Standard Test Method for Measuring the Ability of a Synthetic Polymeric Material to Resist Bacterial Adherence
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ASTM E3371-22 - Standard Test Method for Measuring the Ability of a Synthetic Polymeric Material to Resist Bacterial Adherence
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E3371 − 22
Standard Test Method for
Measuring the Ability of a Synthetic Polymeric Material to
1
Resist Bacterial Adherence
This standard is issued under the fixed designation E3371; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Synthetic polymeric materials, used in packaging for food containers, personal care products and
other items, may have inherent antimicrobial properties. Others may contain antimicrobial additives.
Many methods, such as Test Method E2180 are used to determine quantitative bacterial reductions
caused by these additives. Test Method E3151 specifically examines the ability of tubular, yarn and
fiber specimens to resist bacterial colonization/adherence. However, these methods do not quantita-
tively measure the number of culturable bacteria adhering to the flat surfaces of these synthetic
polymeric materials, as measured in this test method.
1. Scope 1.5 Antimicrobial treated non-porous surfaces may demon-
strate ability to resist bacterial adherence in this method. This
1.1 This method is designed to evaluate (quantitatively) the
method does not purport to differentiate between anti-
number of bacteria attached to the flat, two-dimensional
adherenceandantimicrobialactivitynorisitdesignedtoreflect
surfaces of synthetic polymeric materials and polymeric coat-
specificend-useorenvironmentalconditions.Anyproductthat
ings on various substrates that may or may not contain bound
demonstratesabilitytoresistbacterialadherenceinthismethod
or incorporated anti-adherent agents. The method focuses on
should be measured for antimicrobial activity using a separate
assessing the ability of the surface to reduce bacterial attach-
ment. Other microorganisms such as yeast and fungal conidia test technique such as Test Method E2180 or ISO 22196.
may be tested using this method.
1.6 Themethodfocusesonassessingtheabilityofsynthetic
1.2 This test method quantitatively determines the differ-
polymeric materials and polymeric coatings on various sub-
ences in bacterial adherence seen between synthetic polymeric
strates to reduce bacterial attachment. The specimen with
surfaces that allow bacterial adherence and those that do not,
absorbingoradhesivesurfacesmaybeunabletobedisinfected
comparingthenumberoforganismsrecoveredfromthecontrol
properly before testing, or may trap inoculated organism
surfacetothenumberrecoveredfromthetestspecimensurface
during recovery process and thus lead to a false result. This
after the contact time. Knowledge of microbiological tech-
method does not apply to specimens with absorbent or adhe-
niques is required for these procedures.
sive surfaces.
1.3 Thistestmethodspecifiespropermethodsformeasuring
1.7 The values stated in SI units are to be regarded as
theabilityofasyntheticpolymericmaterialtoresistadherence
standard. The values given in parentheses after SI units are
against specified organism. Due to individual sensitivities, the
providedforinformationonlyandarenotconsideredstandard.
result of one test organism might not be applicable for other
organisms.
1.8 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
1.4 This test method is designed to measure the potential
ability to resist bacterial adherence of a non-porous surface responsibility of the user of this standard to establish appro-
compared directly to a polyester control panel known to priate safety, health, and environmental practices and deter-
support bacterial adherence under specific testing conditions.
mine the applicability of regulatory limitations prior to use.
1.9 This international standard was developed in accor-
dance with internationally recognized principles on standard-
1
This test method is under the jurisdiction of ASTM Committee E35 on ization established in the Decision on Principles for the
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
Development of International Standards, Guides and Recom-
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
mendations issued by the World Trade Organization Technical
Current edition approved Oct. 1, 2022. Published November 2022. DOI:
10.1520/E3371–22. Barriers to Trade (TBT) Committee.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E3371 − 22
2. Referenced
...

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SIGNIFICANCE AND USE
4.1 Regulatory Requirements—The USA Code of Federal Regulations (10CFR Part 50, Appendix H) requires the implementation of a reactor vessel materials surveillance program for all operating LWRs. Other countries have similar regulations. The purpose of the program is to (1) monitor changes in the fracture toughness properties of ferritic materials in the reactor vessel beltline6 resulting from exposure to neutron irradiation and the thermal environment, and (2) make use of the data obtained from surveillance programs to determine the conditions under which the vessel can be operated with adequate margins of safety throughout its service life. Practice E185, derived mechanical property data, and (r, θ, z) physics-dosimetry data (derived from the calculations and reactor cavity and surveillance capsule measurements (1) using physics-dosimetry standards) can be used together with information in Guide E900 and Refs. 4, 11-18 to provide a relation between property degradation and neutron exposure, commonly called a “trend curve.” To obtain this trend curve at all points in the pressure vessel wall requires that the selected trend curve be used together with the appropriate (r, θ, z) neutron field information derived by use of this guide to accomplish the necessary interpolations and extrapolations in space and time.  
4.2 Neutron Field Characterization—The tasks required to satisfy the second part of the objective of 4.1 are complex and are summarized in Practice E853. In doing this, it is necessary to describe the neutron field at selected (r, θ, z) points within the pressure vessel wall. The description can be either time dependent or time averaged over the reactor service period of interest. This description can best be obtained by combining neutron transport calculations with plant measurements such as reactor cavity (ex-vessel) and surveillance capsule or RPV cladding (in-vessel) measurements, benchmark irradiations of dosimeter sensor materials, and knowledge of th...
SCOPE
1.1 This guide establishes the means and frequency of monitoring the neutron exposure of the LWR reactor pressure vessel throughout its operating life.  
1.2 The physics-dosimetry relationships determined from this guide may be used to estimate reactor pressure vessel damage through the application of Practice E693 and Guide E900, using fast neutron fluence (E > 1.0 MeV and E > 0.1 MeV), displacements per atom (dpa), or damage-function-correlated exposure parameters as independent exposure variables. Supporting the application of these standards are the E853, E944, E1005, and E1018 standards, identified in 2.1.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

  • Guide
    12 pages
    English language
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  • Guide
    12 pages
    English language
    sale 15% off