ASTM D4763-06

Standard Practice for Identification of Chemicals in Water by Fluorescence Spectroscopy

SCOPE
1.1 This practice allows for the identification of 90 chemicals that may be found in water or in surface layers on water. This practice is based on the use of room-temperature fluorescence spectra taken from lists developed by the U.S. Environmental Protection Agency and the U.S. Coast Guard (). Ref () is the primary source for these spectra. This practice is also based on the assumption that such chemicals are either present in aqueous solution or are extracted from water into an appropriate solvent.
1.2 Although many organic chemicals containing aromatic rings, heterocyclic rings, or extended conjugated double-bond systems have appreciable quantum yields of fluorescence, this practice is designed only for the specific compounds listed. If present in complex mixtures, preseparation by high-performance liquid chromatography (HPLC), column chromatography, or thin-layer chromatography (TLC) would probably be required.
1.3 If used with HPLC, this practice could be used for the identification of fluorescence spectra generated by optical multichannel analyzers (OMA) or diode-array detectors.
1.4 For simple mixtures, or in the presence of other nonfluorescing chemicals, separatory techniques might not be required. The excitation and emission maximum wavelengths listed in this practice could be used with standard fluorescence techniques (Refs ) to quantitate these ninety chemicals once identification had been established. For such uses, generation of a calibration curve, to determine the linear range for use of fluorescence quantitation would be required for each chemical. Examination of solvent blanks to subtract or eliminate any fluorescence background would probably be required.
This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 30-Jun-2006
• ICS: 13.060.50 - Examination of water for chemical substances
• Technical Committee: D19 - Water
• Drafting Committee: D19.06 - Methods for Analysis for Organic Substances in Water

Relations

• Replaces: ASTM D4763-88(2001) - Standard Practice for Identification of Chemicals in Water by Fluorescence Spectroscopy
• Effective Date: 01-Jul-2006
• Referred By: ASTM D3694-96(2017) - Standard Practices for Preparation of Sample Containers and for Preservation of Organic Constituents
• Effective Date: 01-Jul-2006

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:D4763 −06
StandardPractice for
Identification of Chemicals in Water by Fluorescence
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Spectroscopy
This standard is issued under the fixed designation D4763; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope of the user of this standard to establish appropriate safety and
health practices and determine the applicability of regulatory
1.1 This practice allows for the identification of 90 chemi-
limitations prior to use.
cals that may be found in water or in surface layers on water.
This practice is based on the use of room-temperature fluores-
2. Referenced Documents
cence spectra taken from lists developed by the U.S. Environ-
2 3
mental Protection Agency and the U.S. Coast Guard (1). Ref 2.1 ASTM Standards:
(1) is the primary source for these spectra. This practice is also D1129 Terminology Relating to Water
based on the assumption that such chemicals are either present D1193 Specification for Reagent Water
in aqueous solution or are extracted from water into an E131 Terminology Relating to Molecular Spectroscopy
appropriate solvent. E275 Practice for Describing and Measuring Performance of
Ultraviolet and Visible Spectrophotometers
1.2 Although many organic chemicals containing aromatic
rings, heterocyclic rings, or extended conjugated double-bond
3. Terminology
systems have appreciable quantum yields of fluorescence, this
practice is designed only for the specific compounds listed. If 3.1 Definition—For definitions of terms used in this
practice, refer to Terminology D1129, Specification D1193,
present in complex mixtures, preseparation by high-
performance liquid chromatography (HPLC), column and definitions under the jurisdiction of Committee E-13 such
as Definitions E131 and Practice E275
chromatography, or thin-layer chromatography (TLC) would
probably be required.
4. Summary of Practice
1.3 If used with HPLC, this practice could be used for the
identification of fluorescence spectra generated by optical 4.1 This practice uses well tested fluorescence techniques to
multichannel analyzers (OMA) or diode-array detectors. detect and identify (or determine the absence of) 90 chemicals
that have relatively high fluorescence yields. Table 1 lists for
1.4 For simple mixtures, or in the presence of other non-
each chemical an appropriate solvent (either cyclohexane,
fluorescing chemicals, separatory techniques might not be
water, methyl or ethyl alcohol, depending on solubility), a
required. The excitation and emission maximum wavelengths
suggested excitation wavelength for maximum sensitivity, a
listed in this practice could be used with standard fluorescence
wavelength corresponding to the emission maximum, the
techniques (Refs 2-6)toquantitatetheseninetychemicalsonce
number of fluorescence peaks and shoulders, the width (full
identification had been established. For such uses, generation
width at half of the maximum emission intensity) of the
of a calibration curve, to determine the linear range for use of
strongest fluorescence peak and the detection limit for the
fluorescence quantitation would be required for each chemical.
experimental conditions given. Detection limits could be
Examination of solvent blanks to subtract or eliminate any
lowered, following identification, by using broader slit widths.
fluorescence background would probably be required.
A list of corrected fluorescence spectra for the chemicals
1.5 This standard does not purport to address the safety
included in this practice are also available (1) .
concerns, if any, associated with its use. It is the responsibility
4.2 Identification of the sample is made by comparison of
the obtained spectra with information in Table 1 and by direct
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visual comparison of appropriate spectra with positions of
This practice is under the jurisdiction of ASTM Committee D19 on Water and
is the direct responsibility of Subcommittee D19.06 on Methods for Analysis for
Organic Substances in Water.
Current edition approved July 1, 2006. Published July 2006. Originally approved
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in 1988. Last previous edition approved in 2001 as D4763 – 88 (2001). DOI: For referenced ASTM standards, visit the ASTM website, www.astm.org, or
10.1520/D4763-06. contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM
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The boldface numbers in parentheses refer to the list of references at the end of Standards volume information, refer to the standard’s Document Summary page on
this practice. the ASTM website.
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