ASTM E193-94(2010)

Standard Specification for Laboratory Glass Micropipets

ABSTRACT
This specification describes the design and dimensional requirements for several types of laboratory glass micropipets useful in measuring microlitre quantities of liquids. The types of micropipets covered here are the measuring-type micropipets, Folin-type micrpipets, Pregl-type micro washout pipets, density-type micro weighing pipets, and microlitre pipets.
SCOPE
1.1 This specification covers several micropipets useful in measuring microlitre quantities of liquids.
Note 1—This specification was originally developed by the Committee on Microchemical Apparatus, Division of Analytical Chemistry, American Chemical Society. , ,  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

General Information

• Status: Historical
• Publication Date: 30-Jun-2010
• ICS: 71.040.20 - Laboratory ware and related apparatus
• Technical Committee: E41 - Laboratory Apparatus
• Drafting Committee: E41.01 - Laboratory Ware and Supplies

Relations

• Replaces: ASTM E193-94(2005) - Standard Specification for Laboratory Glass Micropipets
• Effective Date: 01-Jul-2010
• Replaced By: ASTM E193-94(2017) - Standard Specification for Laboratory Glass Micropipets (Withdrawn 2024)
• Effective Date: 15-Nov-2017
• Referred By: ASTM E969-02(2019) - Standard Specification for Glass Volumetric (Transfer) Pipets
• Effective Date: 01-Jul-2010

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation:E193 −94 (Reapproved 2010)
Standard Specification for
Laboratory Glass Micropipets
This standard is issued under the fixed designation E193; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope microlitre pipets in order to differentiate among the various
pipets,notonatechnicalbasis,butratheronthebasisofusage.
1.1 This specification covers several micropipets useful in
This is done to prevent confusion that might result from the
measuring microlitre quantities of liquids.
fact that the pipets shown in Figs. 1 and 2, and Fig. 3 are listed
NOTE 1—This specification was originally developed by the Committee
in trade catalogs as micropipets designed especially for use in
on MicrochemicalApparatus, Division ofAnalytical Chemistry,American
the fields of biological and clinical chemistry, and the pipets
2,3, 4
Chemical Society.
shown in Fig. 4 are listed as micropipets, density-type, de-
1.2 The values stated in SI units are to be regarded as
signed for use as microweighing pipets, or pycnometers.
standard. No other units of measurement are included in this
standard. 4. Micropipets, Measuring-Type (“to deliver”)
2,6
4.1 The measuring-type micropipets are specified in five
2. Referenced Documents
capacities and shall conform to the requirements shown in Fig.
2.1 ASTM Standards:
1. The narrow, elongated delivery stem of the pipets can reach
E920 Specification for Commercially Packaged Laboratory 2,5
to the bottom of microvolumetric flasks, permitting almost
Apparatus
complete withdrawal of the contents of the flask. Quantitative
E921 Specification for Export Packaged Laboratory Appa-
delivery of the entire volume of the pipets is obtained by
ratus
touching off the last drop.
E1133 Practice for Performance Testing of Packaged Labo-
ratory Apparatus for United States Government Procure-
5. Micropipets, Folin-Type (“to contain”)
ments
4,6,7
5.1 The Folin-type micropipets are specified in two
E1157 Specification for Sampling and Testing of Reusable
capacities and shall conform to the requirements shown in Fig.
Laboratory Glassware
2.Completedeliveryofthevolumeindicatedbythegraduation
mark on these pipets is ensured by washing out several times
3. Nomenclature
any solution adhering to the inner surface with wash liquid
3.1 ThepipetsspecifiedinFigs.1-3,andFig.4areclassified
drawn up from the tip.
as micropipets, whereas those in Figs. 5 and 6 are classified as
6. Micro Washout Pipets, Pregl-Type (“to contain”)
4,6,8
This specification is under the jurisdiction of ASTM Committee E41 on
6.1 The micro washout pipets, Pregl-type are specified
Laboratory Apparatus and is the direct responsibility of Subcommittee E41.01 on
in four capacities and shall conform to the requirements shown
Apparatus.
in Fig. 3. Quantitative delivery of the volume indicated by the
Current edition approved July 1, 2010. Published July 2010. Originally approved
in 1962. Last previous edition approved in 2005 as E193 – 94 (2005). DOI:
graduation mark on the pipets is obtained by rinsing out the
10.1520/E0193-94R10.
contents with wash liquid added from the top of the pipet.
Committee on Microchemical Apparatus, Division of Analytical Chemistry,
American Chemical Society. “ Report on Recommended Specifications for Micro-
7. Micro Weighing Pipets, Density-Type (Pycnometers)
chemical Apparatus, Volumetric Glassware, Flasks, Pipets, and Centrifuge Tubes,”
(“to contain”)
Analytical Chemistry, Vol 28, 1956, p. 1993.
Committee on Microchemical Apparatus, Division of Analytical Chemistry,
7.1 The micro weighing pipets, density-type (pycnometers),
American Chemical Society. “ Report on Recommended Specifications for Micro-
4,6,9
chemical Apparatus, Volumetric Glassware, Microlitre Pipets,” Analytical with ground-glass caps, are specified in three capacities
Chemistry, Vol 30, 1958, p. 1702.
Committee on Microchemical Apparatus, Division of Analytical Chemistry,
American Chemical Society. “ Report on Recommended Specifications for Micro- Steyermark,A., Quantitative Organic Microanalysis, 2nd Ed.,Academic Press,
chemical Apparatus, Volumetric Glassware, Micropipets,” Analytical Chemistry, New York, NY, 1961.
Vol 32, 1960, p. 1045. Folin, O., Journal of Biological Chemistry, Vol 77, 1928, p. 421.
5 8
For referenced ASTM standards, visit the ASTM website, www.astm.org, or Grant,J.,QuantitativeOrganicMicroanalysisBasedonMethodsofFritzPregl,
contact ASTM Customer Service at [e-mail]. For Annual Book of ASTM 5th Ed., Blakiston Co., Philadelphia, PA 1951, p. 34.
Standards volume information, refer to the standard’s Document Summary page on Alber, H. K.,IndustrialandEngineeringChemistry,AnalyticalEdition,Vol 12,
the ASTM website. 1940, p. 764.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
E193−94 (2010)
Lining
Subdivision, Interval Graduated, Ring at Each, Half Ring Number at 0 Tolerance, ±,
Capacity, mL mL mL mL at Each, mL and Each mL mL
0.2 0.01 0 to 0.18 0.02 0.01 0.02 0.005
0.5 0.01 0 to 0.45 0.05 0.01 0.1 0.01
1 0.02 0 to 0.90 0.1 0.02 0.1 0.02
2 0.05 0 to 1.75 0.25 0.05 0.5 0.04
3 0.05 0 to 2.70 0.25 0.05 0.5 0.06
Notes:
1. No graduations shall appear in tapered portion.
2. Tip may be tapered at junction with body, but outside diameter at this point may not exceed 4.5 mm.
3. Tip outlet shall be glazed, with le
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