ASTM E1200-87(2012)

Standard Practice for Preserving Zooplankton Samples

SIGNIFICANCE AND USE
4.1 Calcium Carbonate (CaCO3) buffered formalin (3 to 5 %) can be used as a permanent preservative for zooplankton. Lugol's iodine solution can be used to preserve zooplankton for up to one year. Thirty percent ethanol, 30 % glutaraldehyde, or 25 % vinegar (can use 3 % acidic acid solution) can be used for more temporary storage and preservation of zooplankton samples. A 25 % vinegar solution is preferred to preserve soft-bodied planktonic coelenterates.
SCOPE
1.1 This practice describes the proper procedures for preserving zooplankton samples with either formaldehyde, ethanol, glutaraldehyde, Lugol's iodine solution, or vinegar (acetic acid).  
1.2 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 30-Nov-2012
• ICS: 13.060.30 - Sewage water
• Technical Committee: D19 - Water
• Drafting Committee: D19.24 - Water Microbiology

Relations

• Replaced By: ASTM E1200-19 - Standard Practice for Preserving Zooplankton Samples
• Effective Date: 01-Apr-2019
• Referred By: ASTM E1199-19 - Standard Practice for Sampling Zooplankton with a Clarke-Bumpus Plankton Sampler
• Effective Date: 01-Dec-2012
• Referred By: ASTM E1198-19 - Standard Practice for Sampling Zooplankton with Pumps
• Effective Date: 01-Dec-2012

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E1200 − 87 (Reapproved 2012)
Standard Practice for
Preserving Zooplankton Samples
This standard is issued under the fixed designation E1200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope all reagents shall conform to the specifications of the Commit-
tee onAnalytical Reagents of theAmerican Chemical Society,
1.1 This practice describes the proper procedures for pre-
where such specifications are available.
serving zooplankton samples with either formaldehyde,
ethanol, glutaraldehyde, Lugol’s iodine solution, or vinegar 5.2 Purity of Water—Unless otherwise indicated, references
(acetic acid). to water shall be understood to mean reagent water as defined
by Type II of Specification D1193.
1.2 This standard does not purport to address all of the
safety problems, if any, associated with its use. It is the
5.3 Formaldehyde Solution—37 to 40 % aqueous.
responsibility of the user of this standard to establish appro-
5.4 Ethanol (95 %)—Dilute with water 30 mL of ethanol to
priate safety and health practices and determine the applica-
100 mL.
bility of regulatory limitations prior to use.
5.5 Glutaraldehyde—Dilute with water 30 mL of glutaral-
2. Referenced Documents
dehyde to 100 mL.
2.1 ASTM Standards:
5.6 Lugol’s Iodine Solution—Dissolve 60 g of potassium
D1193 Specification for Reagent Water
iodide and 40 g of iodine crystals in 1000 mL of water.
5.7 Vinegar Solution—Dilute 25 or 250 mL of vinegar to
3. Summary of Practice
100 or 1000 mL of water, respectively. An alternative is to
3.1 Azooplankton sample collected with either a qualitative
dilute with water 3 mL of 100 % acetic acid to 100 mL.
or quantitative sampler is preserved with formaldehyde,
5.8 DetergentSolution (20 %)—Dilute with water 20 mLof
ethanol, glutaraldehyde, Lugol’s iodine solution, or 25 %
household liquid detergent to 100 mL.
vinegar or 3 % acetic acid, as dictated by needs of the study.
The preservatives are listed in order of preference.
6. Procedure
4. Significance and Use
6.1 If the sample is to be examined within 2 to 3 h after
collection, no special treatment is necessary. A zooplankton
4.1 Calcium Carbonate (CaCO ) buffered formalin (3 to
sample may be maintained longer if refrigerated or iced at 2 to
5 %) can be used as a permanent preservative for zooplankton.
3°C. For extended storage, beyond 72 h, preservation is
Lugol’s iodine solution can be used to preserve zooplankton
required. There are numerous preservatives for zooplankton.
foruptooneyear.Thirtypercentethanol,30 %glutaraldehyde,
The formaldehyde solution is the most commonly used.
or 25 % vinegar (can use 3 % acidic acid solution) can be used
for more temporary storage and preservation of zooplankton
6.2 To use the formaldehyde solution, to each 1000 mL of
samples. A 25 % vinegar solution is preferred to preserve
sample add 30 mL of 37 to 40 % aqueous formaldehyde
soft
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