ASTM E1200-19
(Practice)Standard Practice for Preserving Zooplankton Samples
Standard Practice for Preserving Zooplankton Samples
SIGNIFICANCE AND USE
4.1 Calcium Carbonate (CaCO3) buffered formalin (3 to 5 %) can be used as a permanent preservative for zooplankton. Lugol’s iodine solution can be used to preserve zooplankton for up to one year. Thirty percent ethanol, 30 % glutaraldehyde, or 25 % vinegar (can use 3 % acetic acid solution) can be used for more temporary storage and preservation of zooplankton samples. A 25 % vinegar solution is preferred to preserve soft-bodied planktonic coelenterates.
SCOPE
1.1 This practice describes the proper procedures for preserving zooplankton samples with either formaldehyde, ethanol, glutaraldehyde, Lugol’s iodine solution, or vinegar (acetic acid).
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E1200 − 19
Standard Practice for
1
Preserving Zooplankton Samples
This standard is issued under the fixed designation E1200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope Lugol’siodinesolutioncanbeusedtopreservezooplanktonfor
up to one year. Thirty percent ethanol, 30 % glutaraldehyde, or
1.1 This practice describes the proper procedures for pre-
25 % vinegar (can use 3 % acetic acid solution) can be used for
serving zooplankton samples with either formaldehyde,
more temporary storage and preservation of zooplankton
ethanol, glutaraldehyde, Lugol’s iodine solution, or vinegar
samples. A 25 % vinegar solution is preferred to preserve
(acetic acid).
soft-bodied planktonic coelenterates.
1.2 The values stated in SI units are to be regarded as
standard. No other units of measurement are included in this
5. Reagents
standard.
5.1 Purity of Reagents—Reagent grade chemicals shall be
1.3 This standard does not purport to address all of the
used in all tests. Unless otherwise indicated, it is intended that
safety concerns, if any, associated with its use. It is the
all reagents shall conform to the specifications of the Commit-
responsibility of the user of this standard to establish appro-
tee onAnalytical Reagents of theAmerican Chemical Society,
3
priate safety, health, and environmental practices and deter-
where such specifications are available.
mine the applicability of regulatory limitations prior to use.
5.2 Purity of Water—Unless otherwise indicated, references
1.4 This international standard was developed in accor-
to water shall be understood to mean reagent water as defined
dance with internationally recognized principles on standard-
by Type II of Specification D1193.
ization established in the Decision on Principles for the
5.3 Formaldehyde Solution—37 to 40 % aqueous.
Development of International Standards, Guides and Recom-
(Warning—Possible carcinogen.)
mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
5.4 Ethanol (95 %)—Dilute with water 30 mL of ethanol to
100 mL.
2. Referenced Documents
5.5 Glutaraldehyde—Dilute with water 30 mL of glutaral-
2
2.1 ASTM Standards:
dehyde to 100 mL.
D1193 Specification for Reagent Water
5.6 Lugol’s Iodine Solution—Dissolve 60 g of potassium
3. Summary of Practice iodide and 40 g of iodine crystals in 1000 mL of water.
3.1 Azooplankton sample collected with either a qualitative 5.7 Vinegar Solution—Dilute 25 or 250 mL of vinegar to
or quantitative sampler is preserved with formaldehyde, 100 or 1000 mL of water, respectively. An alternative is to
ethanol, glutaraldehyde, Lugol’s iodine solution, or 25 % dilute with water 3 mL of 100 % acetic acid to 100 mL.
vinegar or 3 % acetic acid, as dictated by needs of the study.
5.8 Detergent Solution (20 %)—Dilute with water 20 mLof
The preservatives are listed in order of preference.
household liquid detergent to 100 mL.
4. Significance and Use
6. Procedure
4.1 Calcium Carbonate (CaCO ) buffered formalin (3 to
3
6.1 If the sample is to be examined within 2 to 3 h after
5 %) can be used as a permanent preservative for zooplankton.
collection, no special treatment is nece
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E1200 − 87 (Reapproved 2012) E1200 − 19
Standard Practice for
1
Preserving Zooplankton Samples
This standard is issued under the fixed designation E1200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This practice describes the proper procedures for preserving zooplankton samples with either formaldehyde, ethanol,
glutaraldehyde, Lugol’sLugol’s iodine solution, or vinegar (acetic acid).
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety problems,concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and
determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
3. Summary of Practice
3.1 A zooplankton sample collected with either a qualitative or quantitative sampler is preserved with formaldehyde, ethanol,
glutaraldehyde, Lugol’sLugol’s iodine solution, or 25 % vinegar or 3 % acetic acid, as dictated by needs of the study. The
preservatives are listed in order of preference.
4. Significance and Use
4.1 Calcium Carbonate (CaCO ) buffered formalin (3 to 5 %) can be used as a permanent preservative for zooplankton.
3
Lugol’sLugol’s iodine solution can be used to preserve zooplankton for up to one year. Thirty percent ethanol, 30 %
glutaraldehyde, or 25 % vinegar (can use 3 % acidicacetic acid solution) can be used for more temporary storage and preservation
of zooplankton samples. A 25 % vinegar solution is preferred to preserve soft-bodied planktonic coelenterates.
5. Reagents
5.1 Purity of Reagents—Reagent grade chemicals shall be used in all tests. Unless otherwise indicated, it is intended that all
reagents shall conform to the specifications of the Committee on Analytical Reagents of the American Chemical Society, where
3
such specifications are available.
5.2 Purity of Water—Unless otherwise indicated, references to water shall be understood to mean reagent water as defined by
Type II of Specification D1193.
5.3 Formaldehyde Solution—37 to 40 % aqueous. (Warning—Possible carcinogen.)
5.4 Ethanol (95 %)—Dilute with water 30 mL of ethanol to 100 mL.
5.5 Glutaraldehyde—Dilute with water 30 mL of glutaraldehyde to 100 mL.
1
This practice is under the jurisdiction of ASTM Committee D19 on Water and is the direct responsibility of Subcommittee D19.24 on Water Microbiology.
Current edition approved Dec. 1, 2012April 1, 2019. Published December 2012April 2019. Originally approved in 1987. Last previous edition approved in 20042012 as
E1200 – 87 (2004).(2012). DOI: 10.1520/E1200-87R12.10.1520/E1200-19.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’sstandard’s Document Summary page on the ASTM website.
3
“ReagentReagent Chemicals, American Chemical Society Specifications,”Specifications, Am.American Chemical Soc.,Society, Washington, D.C.DC. For suggestions on
the testing of reagents not listed by the American Chemical Society, see “AnalarAnalar Standards for Laboratory U.K.Chemicals, Chemicals,” BDH Ltd., Poole, Dorset, U.K.,
and the “United States Pharmacopeia.”United States Pharmacopeia and National Formulary, U.S. Pharmacopeial Convention, Inc. (USPC), Rockville, MD.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
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E1200 − 19
5.6 Lugol’sLugol’s Iodine Solution—Dissolve 60 g of potassium iodide and 40 g of iodine crystals in 1000 mL of water.
5.7 Vinegar Solution—Dilute 25 or 250 mL of vinegar to 100 or 1000 mL of water, respectively. An alternative is to dilute wi
...
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E1200 − 19
Standard Practice for
1
Preserving Zooplankton Samples
This standard is issued under the fixed designation E1200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope Lugol’s iodine solution can be used to preserve zooplankton for
up to one year. Thirty percent ethanol, 30 % glutaraldehyde, or
1.1 This practice describes the proper procedures for pre-
25 % vinegar (can use 3 % acetic acid solution) can be used for
serving zooplankton samples with either formaldehyde,
more temporary storage and preservation of zooplankton
ethanol, glutaraldehyde, Lugol’s iodine solution, or vinegar
samples. A 25 % vinegar solution is preferred to preserve
(acetic acid).
soft-bodied planktonic coelenterates.
1.2 The values stated in SI units are to be regarded as
standard. No other units of measurement are included in this
5. Reagents
standard.
5.1 Purity of Reagents—Reagent grade chemicals shall be
1.3 This standard does not purport to address all of the
used in all tests. Unless otherwise indicated, it is intended that
safety concerns, if any, associated with its use. It is the
all reagents shall conform to the specifications of the Commit-
responsibility of the user of this standard to establish appro-
tee on Analytical Reagents of the American Chemical Society,
3
priate safety, health, and environmental practices and deter-
where such specifications are available.
mine the applicability of regulatory limitations prior to use.
5.2 Purity of Water—Unless otherwise indicated, references
1.4 This international standard was developed in accor-
to water shall be understood to mean reagent water as defined
dance with internationally recognized principles on standard-
by Type II of Specification D1193.
ization established in the Decision on Principles for the
5.3 Formaldehyde Solution—37 to 40 % aqueous.
Development of International Standards, Guides and Recom-
(Warning—Possible carcinogen.)
mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
5.4 Ethanol (95 %)—Dilute with water 30 mL of ethanol to
100 mL.
2. Referenced Documents
5.5 Glutaraldehyde—Dilute with water 30 mL of glutaral-
2
2.1 ASTM Standards:
dehyde to 100 mL.
D1193 Specification for Reagent Water
5.6 Lugol’s Iodine Solution—Dissolve 60 g of potassium
3. Summary of Practice iodide and 40 g of iodine crystals in 1000 mL of water.
3.1 A zooplankton sample collected with either a qualitative 5.7 Vinegar Solution—Dilute 25 or 250 mL of vinegar to
or quantitative sampler is preserved with formaldehyde, 100 or 1000 mL of water, respectively. An alternative is to
ethanol, glutaraldehyde, Lugol’s iodine solution, or 25 % dilute with water 3 mL of 100 % acetic acid to 100 mL.
vinegar or 3 % acetic acid, as dictated by needs of the study.
5.8 Detergent Solution (20 %)—Dilute with water 20 mL of
The preservatives are listed in order of preference.
household liquid detergent to 100 mL.
4. Significance and Use
6. Procedure
4.1 Calcium Carbonate (CaCO ) buffered formalin (3 to
3
6.1 If the sample is to be examined within 2 to 3 h after
5 %) can be used as a permanent preservative for zooplankton.
collection, no special treatment is necessary. A zooplankton
sample may be maintained longer if refrigerated or iced at 2 to
...
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