EN 17701-1:2024
(Main)Plant biostimulants - Determination of specific elements - Part 1: Digestion by aqua regia for subsequent determination of elements
Plant biostimulants - Determination of specific elements - Part 1: Digestion by aqua regia for subsequent determination of elements
This document specifies the method for the digestion of different plant biostimulants with aqua regia to enable a subsequent determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), mercury (Hg), nickel (Ni), lead (Pb) and zinc (Zn). The method can be also applied for determination of other elements. The method is applicable for all solid and/or liquid plant biostimulants.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers, Growing Media, Inhibitors, Plant Biostimulants, and where the following category Plant Biostimulants is the highest % in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants is not the highest % in the blend, the European Standard for the highest % of the blend applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the component EU fertilising products used for the blend have identical formulations , the user decides which standard to apply.
The digests are suitable for analysis using EN 17701-2:2024 (ICP-AES) and EN 17701-3:2024 (Hg analysis).
NOTE Alternatively, inductively coupled plasma mass spectrometry (ICP-MS) can be used for the determination of the elements in the aqua regia digests if the user proves that the method gives the same results.
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 1: Aufschluss durch Königswasser zur anschließenden Bestimmung der Elemente
Dieses Dokument legt das Verfahren für den Aufschluss verschiedener Pflanzen-Biostimulanzien mit Königswasser fest, um eine anschließende Bestimmung von Arsen (As), Cadmium (Cd), Kupfer (Cu), Chrom (Cr), Quecksilber (Hg), Nickel (Ni), Blei (Pb) und Zink (Zn) zu ermöglichen. Das Verfahren kann auch für die Bestimmung anderer Elemente verwendet werden. Das Verfahren ist für alle festen und/oder flüssigen Pflanzen-Biostimulanzien anwendbar.
Dieses Dokument ist für die Mischungen von Düngeprodukten anwendbar, in denen eine Mischung aus mindestens zwei der folgenden Kategorien von Komponenten-EU Düngeprodukten besteht: Düngemittel, Kalkdünger, Bodenverbesserungsmittel, Kultursubstrate, Hemmstoffe, Pflanzen-Biostimulanzien und wenn die folgende Kategorie Pflanzen-Biostimulanzien prozentual den höchsten Massen- oder Volumenanteil in der Mischung bzw., bei flüssiger Form, den höchsten Trockenmasseanteil aufweist. Wenn Pflanzen-Biostimulanzien nicht den höchsten prozentualen Anteil in der Mischung ausmachen, ist die für den höchsten prozentualen Anteil geltende Europäische Norm anwendbar. Falls eine Düngemittelmischung aus Komponenten gleicher Menge besteht oder falls die für die Mischung verwendeten Komponenten-EU Düngeprodukte identische Formulierungen haben, entscheidet der Anwender, welche Norm angewendet wird.
Die Aufschlüsse sind für die Analyse nach EN 17701-2:2024 (ICP AES) und EN 17701-3:2024 (Quecksilber-Analyse) geeignet.
ANMERKUNG Alternativ kann die Massenspektrometrie mit induktiv gekoppeltem Plasma (ICP MS) für die Bestimmung der Elemente in den Königswasseraufschlüssen genutzt werden, sofern der Anwender nachweist, dass das Verfahren zu den gleichen Ergebnissen führt.
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 1 : Digestion à l’eau régale pour le dosage ultérieur des éléments
Le présent document spécifie la méthode pour la digestion de différents biostimulants des végétaux à l’eau régale pour permettre le dosage ultérieur de l’arsenic (As), du cadmium (Cd), du cuivre (Cu), du chrome (Cr), du mercure (Hg), du nickel (Ni), du plomb (Pb) et du zinc (Zn). Cette méthode peut aussi être appliquée pour le dosage d’autres éléments. La méthode est applicable à tous les biostimulants des végétaux solides et/ou liquides.
Le présent document est applicable aux mélanges de fertilisants constitués d’au moins deux composants appartenant aux catégories de fertilisants UE suivantes : engrais, amendements minéraux basiques, amendements du sol, supports de culture, inhibiteurs, biostimulants des végétaux et, dans lesquels la catégorie Biostimulants des végétaux représente le pourcentage le plus élevé du mélange en masse ou en volume, ou dans le cas d’un liquide, en masse sèche. Si les biostimulants des végétaux ne représentent pas le pourcentage le plus élevé du mélange, la Norme européenne relative au composant représentant le pourcentage le plus élevé du mélange s’applique. Dans le cas où un mélange de fertilisants contient des composants en quantité égale ou dans le cas où les composants des fertilisants UE utilisés pour le mélange ont des formulations identiques, l’utilisateur décide quelle norme appliquer.
Les digestats conviennent pour une analyse selon l’EN 17701-2:2024 (ICP-AES) et l’EN 17701-3:2024 (analyse du Hg).
NOTE Il est également possible d’utiliser la spectrométrie de masse avec plasma à couplage inductif (ICP-MS) pour le dosage des éléments dans les digestats à l’eau régale, si l’utilisateur prouve que la méthode conduit aux mêmes résultats.
Rastlinski biostimulanti - Določanje specifičnih elementov - 1. del: Razklop z zlatotopko za določanje elementov
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-februar-2025
Nadomešča:
SIST-TS CEN/TS 17701-1:2023
Rastlinski biostimulanti - Določanje specifičnih elementov - 1. del: Razklop z
zlatotopko za določanje elementov
Plant biostimulants - Determination of specific elements - Part 1: Digestion by aqua regia
for subsequent determination of elements
Pflanzen-Biostimulanzien - Bestimmung spezifischer Elemente - Teil 1: Aufschluss durch
Königswasser zur anschließenden Bestimmung der Elemente
Biostimulants des végétaux - Dosage des éléments spécifiques - Partie 1 : Digestion à
l’eau régale pour le dosage ultérieur des éléments
Ta slovenski standard je istoveten z: EN 17701-1:2024
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 17701-1
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2024
EUROPÄISCHE NORM
ICS 65.080 Supersedes CEN/TS 17701-1:2022
English Version
Plant biostimulants - Determination of specific elements -
Part 1: Digestion by aqua regia for subsequent
determination of elements
Biostimulants des végétaux - Dosage des éléments Pflanzen-Biostimulanzien - Bestimmung spezifischer
spécifiques - Partie 1 : Digestion à l'eau régale pour le Elemente - Teil 1: Aufschluss durch Königswasser zur
dosage ultérieur des éléments anschließenden Bestimmung der Elemente
This European Standard was approved by CEN on 26 August 2024.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17701-1:2024 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Sampling and sample preparation . 6
6 Reagents . 6
7 Apparatus . 6
8 Procedure . 6
8.1 Sample digestion . 6
8.2 Sample pre-concentration . 7
8.3 Blank . 7
9 Test report . 7
Annex A (informative) Adjustment to different digestion procedures and measurement
apparatuses . 9
A.1 Adjustment of the procedure to different final volumes . 9
A.2 Calculation of the sample mass . 9
Bibliography . 11
European foreword
This document (EN 17701-1:2024) has been prepared by Technical Committee CEN/TC 455 “Plant
biostimulants”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2025, and conflicting national standards shall be
withdrawn at the latest by May 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes CEN/TS 17701-1:2022.
CEN/TS 17701-1:2022:
— better explanation of the scope regarding blends;
— addition of normative references.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
EN 17701 series, Plant biostimulants — Determination of specific elements, consists of the following parts:
— Part 1: Digestion by aqua regia for subsequent determination of elements;
— Part 2: Determination of total content of Cd, Pb, Ni, As, Cr, Cu and Zn;
— Part 3: Determination of mercury.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
The European Committee for Standardization (CEN) was requested by the European Commission (EC) to
draft European Standards or European Standardization deliverables to support the implementation of
Regulation (EU) 2019/1009 of 5 June 2019 [1] laying down rules on the making available on the market
of EU fertilising products (“FPR” or “Fertilising Products Regulation”).
This standardization request, presented as SR M/564 and relevant amendments, also contributes to the
Communication on “Innovating for Sustainable Growth: A Bio economy for Europe”. The interest in plant
biostimulants has increased significantly in Europe as a valuable tool to use in agriculture.
Standardization was identified as having an important role in order to promote the use of biostimulants.
The work of CEN/TC 455 seeks to improve the reliability of the supply chain, thereby improving the
confidence of farmers, industry, and consumers in biostimulants, and will promote and support
commercialisation of the European biostimulant industry.
Aqua regia is applied to digest different matrices for the subsequent determination of many elements.
For example, a multi-matrix standard for aqua regia extraction of soils, sludges and biowaste was
prepared by CEN/TC 444 “Environmental characterization of solid matrices”[2, 3]. A similar procedure
was applied for determination of aqua regia extractable contents of arsenic, mercury, cadmium,
chromium, nickel and lead in fertilizers and liming materials [4, 5, 6], for extraction of micronutrients
from mineral fertilizers [7]. CEN/TC 223 “Soil improvers and growing media” published a standard for a
similar procedure for soil improvers and growing media [8].
Wide use of the aqua regia digestion, availability of the instruments and the possibility to merge the
standards for different matrices in future, were the main reasons for also applying this method of
digestion for plant biostimulants. A simple pre-concentration procedure is a part of this document to also
cover the digestion of liquid samples with low dry matter content in the case that the measurement
method is not sufficiently sensitive. Annex A describes in detail the calculation of the initial sample mass
to be taken for the pre-concentration step to achieve concentrations of the individual elements above the
limit of quantification (LOQ).
WARNING — Persons using this document should be familiar with usual laboratory practice. This
document does not purport to address all of the safety issues, if any, associated with its use. It is the
responsibility of the user to establish appropriate health and safety practices and to ensure compliance
with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this document are carried out
by suitably trained staff.
1 Scope
This document specifies the method for the digestion of different plant biostimulants with aqua regia to
enable a subsequent determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), mercury
(Hg), nickel (Ni), lead (Pb) and zinc (Zn). The method can be also applied for determination of other
elements. The method is applicable for all solid and/or liquid plant biostimulants.
This document is applicable to the blends of fertilizing products where a blend is a mix of at least two of
the following component EU fertilising products categories: Fertilizers, Liming Materials, Soil Improvers,
Growing Media, Inhibitors, Plant Biostimulants, and where the following category Plant Biostimulants is
the highest % in the blend by mass or volume, or in the case of liquid form by dry mass. If Plant
Biostimulants is not the highest % in the blend, the European Standard for the highest % of the blend
applies. In case a blend of fertilizing products is composed of components in equal quantity or in case the
component EU fertilising products used for the blend have identical formulations , the user decides
which standard to apply.
The digests are suitable for analysis using EN 17701-2:2024 (ICP-AES) and EN 17701-3:2024
(Hg analysis).
NOTE Alternatively, inductively coupled plasma mass spectrometry (ICP-MS) can be used for the
determination of the elements in the aqua regia digests if the user proves that the method gives the same results.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17702-1:2024, Plant biostimulants — Sampling and sample preparation — Part 1: Sampling
EN 17702-2:2024, Plant biostimulants — Sampling and sample preparation — Part 2: Sample preparation
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp/
— IEC Electropedia: available at https://www.electropedia.org/
3.1
digestion
mineralization of the organic matter of a sample and dissolution of its mineral part, more or less
completely, when reacting with a reagent mixture
3.2
blank test solution
solution prepared in the same way as the test sample solution but omitting the test portion
An example of such a blend is a product with two claimed functions consisting of a non-microbial plant
biostimulant and an organic fertilizer composed of 1kg/kg of plant biostimulant from seaweed.
4 Principle
The samples are digested by boiling in aqua regia for two hours under reflux conditions.
5 Sampling and sample preparation
Sampling and sample preparation are not part of this document. Sampling method and sample
preparation method, given in EN 17702-1:2024 and EN 17702-2:2024 shall be used.
6 Reagents
The concentration of the analyte or interfering substances in the reagents and the water should be
negligible compared to the lowest concentration to be determined.
6.1 Water with a specific conductivity not higher than 0,2 mS/m at 25 °C, free from the elements to be
determined.
6.2 Hydrochloric acid, c(HCl) ≈ 12 mol/l, ρ ≈ 1,18 g/ml.
6.3 Nitric acid, c(HNO ) ≈ 14,3 mol/l, ρ ≈ 1,
...
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