M/564 AMD 1 - Fertilizers Amd1

This document specifies methods for the determination of quantity of the following solid and liquid fertilizing products: organic fertilizers, organo-mineral fertilizers, inorganic fertilizers, liming materials and inhibitors in packages, containers or in bulk.
This document is applicable to fertilizing products blends where organic fertilizers, organo-mineral fertilizers, inorganic fertilizers, liming materials and inhibitors are the highest % of the blend by mass or volume, or in the case of liquid form by dry mass. If organic fertilizers, organo-mineral fertilizers, inorganic fertilizers, liming materials and inhibitors are not the highest % of the blend, the European Standard for the highest % of the blend applies. In case a fertilizing product blend is made up of components in equal quantity, the user decides which standard to apply.
This document is not applicable to the quantity determination of: growing media, soil improvers and plant biostimulants.

This document specifies a method for the determination of nitrogen content in IBDU (isobutylidenediurea) and CDU (crotonylidenediurea) using high-performance liquid chromatography (HPLC).
The method is applicable to all fertilizers which do not contain interfering organic compounds.

This document specifies a method for the determination of phosphorus in fertilizer extracts.
The method is applicable to all extracts of fertilizers for the determination of the different forms of phosphorus as phosphorus soluble in mineral acids, water-soluble phosphorus, phosphorus soluble in solutions of neutral ammonium citrate, phosphorus soluble in 2 % citric acid and phosphorus soluble in a mass fraction of 2 % formic acid.
The method has only been validated on inorganic fertilizers but can be applicable to all extracted phosphorous if proper extraction methods are used.

This document specifies a method for the determination of cyanamide nitrogen in fertilizers. The method is applicable to calcium cyanamide and mixtures of calcium cyanamide with calcium nitrate.

This document specifies a method for the determination of total nitrogen in nitrate-free calcium cyanamide.

This document specifies a method for the determination of total nitrogen in calcium cyanamide. The method is applicable to calcium cyanamide containing nitrates.

TThis document specifies a complexometric method for the determination of the calcium content and the magnesium content of liming materials.
It is not applicable to products with a mass fraction less than 2 % magnesium or those with a mass fraction more than 1 % P2O5 and is not applicable to silicate liming materials.

This document is applicable to liming materials, which contain oxides, hydroxides, carbonates or silicates of the nutrients calcium (Ca) or magnesium (Mg) and the function of which is to correct soil acidity. It is applicable to fertilizing products, which are classified as PFC 2 or the PFC 2 component in PFC 7 of Regulation (EU) 2019/1009 [3].
NOTE   The methods referred to in this document have not been tested for fertilizing product blends (PFC 7) [3].
This document specifies references to the methods for the determination of the following physical and chemical properties and specific contaminants in liming materials:
-   Determination of the cadmium content;
-   Determination of the chromium VI content;
-   Determination of the mercury content;
-   Determination of the nickel content;
-   Determination of the lead content;
-   Determination of the arsenic content;
-   Determination of the total chromium content;
-   Determination of neutralizing value;
-   Determination of the reactivity;
-   Determination of the grain size/granulometry;
-   Determination of the total CaO content;
-   Determination of the total MgO content.
This document does not specify references to the methods for the determination of the following physical and chemical properties and specific contaminants in liming materials, as they are covered in other European Standards:
-   Determination of the copper and zinc content;
-   Determination of the chloride content;
-   Determination of quantity (indicated by mass or volume);
-   Determination of the phosphonates content.

This document specifies general rules to determine the concentration of microorganisms present in plant biostimulants.
The method is applicable to microbial plant biostimulants for verifying that the concentration of microorganisms does not exceed the respective limits outlined in the EU Regulation on Fertilising Products [1].
This horizontal method might not be appropriate in every detail for certain products. In this case, it is necessary to refer to the methodology of specific determination and quantification of the microorganisms.

This document specifies sampling plans and methods of representative sampling of inorganic fertilizers, liming materials and inhibitors for physical and chemical analysis, from packages and containers up to and including 1 000 kg, in liquid and solid form. This document covers sampling of products in bulk only while in motion.
NOTE 1   The sampling of bulk heaps of specified types of fertilizers is covered in prEN 1482 3. Sampling for detection of microbial presence is covered by prEN 1482 4.
NOTE 2   The term product is used throughout the body of this document and is understood to include inorganic fertilizers, liming materials and inhibitors unless otherwise indicated.
It is applicable to the sampling of batches of fertilizer, liming material and inhibitors supplied or ready for supply to third parties, as such, or in smaller batches, each of which would be subject to local, national or regional legislation.
This document does not cover complete, statistical sampling plans.
This document is also applicable to the blends of products where inorganic fertilizers, liming materials, or inhibitors are the main part of the blend in quantity. If fertilizers, liming materials, or inhibitors are not the main part of the blend, the European Standard for the main part of the blend applies. In case a blend of fertilizing products is composed of parts in equal quantity, the user decides which standard to apply. Special care is needed to ensure that the blend is/stays homogeneous and well mixed when sampled.
NOTE 3   It is the responsibility of manufacturers, importers and sellers, however, to ensure they supply a product that complies with its label declaration at the moment of delivery and fulfils the expectations of the end user at the moment of application.

This document specifies methods for the reduction and preparation of samples of fertilizers, liming materials, inhibitors and blends and sets out the requirements for sample preparation reports. It also specifies methods for the preparation of test samples and test portions from laboratory samples of fertilizer for subsequent chemical or physical analysis. It does not cover the preparation of samples for certain physical tests which require test portions of more than 2 kg.
NOTE 1   The term “fertilizer” is used throughout the body of this document and is understood to include liming materials and inhibitors unless otherwise indicated.
NOTE 2   In relation to the procedures set out in this part of the standard any special procedures specific to a particular test method will be set out in that method standard.

This document is applicable to the sampling of the following solid inorganic fertilizers and liming materials supplied or ready for supply, and stored in static heaps:
-   Single nutrient fertilizers,
-   Uniform complex fertilizers,
-   Milled, granulated or dredged liming materials,
-   Any other materials deemed suitable for sampling by the method described in this part of the standard,
for the purpose of testing for compliance with legal requirements and other descriptions and declarations.
NOTE 1   The term “fertilizer” is used throughout the body of this document and includes liming materials and inhibitors unless otherwise indicated.
NOTE 2   Manufacturers, importers and sellers might choose to use this method to obtain samples of other products or blends as well as long as both parties to a transaction agree. The build-up of a static heap often leads to granulometric segregation, which makes the collection of a truly representative sample unlikely.
NOTE 3   It is the responsibility of manufacturers, importers and sellers, however, to ensure they supply a product that complies with its label declaration at the moment of delivery and fulfils the expectations of the end user at the moment of application.

This document specifies the description of the physical unit in organic, organo-mineral and inorganic fertilizers.

This European Standard establishes the methodology for the determining chlorides in plant biostimulant products in the absence of organic material, in accordance with the Regulation of EU fertilising products.

This document specifies a method for determination of the content of mercury (Hg) in plant biostimulants using (cold) vapour generation apparatus coupled to an atomic absorption spectrophotometer and a method using a direct amalgamation technique. It is applicable to aqua regia digests prepared according to EN 17701 1:—1.
NOTE   It is also possible to use other suitable methods for the determination of mercury described in Annex A if users prove that the method gives the same results as the methods described in this document.
This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the method for the main part of the blend applies.

This document provides a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after anaerobic incubation at 30 °C.
The method is applicable to microbial plant biostimulants, except those composed of aerobic bacteria, for verifying that the concentration of anaerobes does not exceed the respective limits outlined in the EU Fertilizers Regulation [1].
This method does not apply to the microbiological monitoring of the environment in which microbial plant biostimulants are manufactured.
No information about potential human pathogens can be inferred from anaerobic plate counts.

This document provides a method for verifying that the pathogen Shigella spp. is not present in microbial plant biostimulants in a concentration that exceeds the respective limits outlined in the EU Regulation on Fertilising Products.
The detection method for Shigella pathogens is not sensitive and quantification is rarely performed. Detection is usually performed using an enrichment medium followed by subculturing onto a variety of selective media.

This document provides guidance for justifying abiotic stress tolerance claim of plant biostimulants used in agriculture.
This document is aimed primarily at manufacturers, laboratories, companies which will put the products on the market, notifying authorities, notified bodies, and market surveillance authorities.
To be in compliance with this standard, it is important also to follow the Recommendations and Quality Criteria described in the Standard of General Principles EN 17700 1:—1.

This document provides the methodology for the enumeration and determination of Azospirillum spp. in Plant Biostimulant products in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council  [1].

This document provides guidance for justifying quality traits claims of plant biostimulants used in agriculture.
This document is aimed primarily at manufacturers, laboratories, companies which will put the products on the market, notifying authorities, notified bodies, and market surveillance authorities.
To be in compliance with this standard, it is important also to follow the Recommendations and Quality Criteria described in the Standard of General Principles EN 17700 1:—1.

This document specifies a method for laboratory measurement of the pH value in liquid microbial plant biostimulants, using pH electrodes with a glass membrane.
Plant biostimulants other than microbial plant biostimulants are excluded from the scope of this document because there is no essential requirement in the Regulation [1] for measuring the pH of non-microbial plant biostimulants.

This document gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in technical and formulated biostimulant products, both in liquid and solid state, and also the horizontal method for the enumeration of ß-glucuronidase-positive Escherichia coli in plant biostimulants products (both in liquid and solid state).
The qualitative method described in this document is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods can be appropriate, depending on the level of detection required.
NOTE   For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).
The quantitative method described in this document uses a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme ß-glucuronidase.
WARNING - Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are ß-glucuronidase negative, such as Escherichia coli O157, will not be detected.

This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulants intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C ± 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilisers Regulation [1].
If necessary, yeast and mould enumerated can be identified using suitable identification tests.

This document describes a method for the detection of Salmonella spp. in biostimulants of the following Product Function Categories (PFCs) and Component Material Category (CMC) of EU fertilizing products, as described in Regulation (EU) 2019/1009 of the European Parliament and of the Council [1]:
-   PFC 6(A): Microbial plant biostimulant;
-   PFC 6(B): Non-microbial plant biostimulant;
-   CMC 7: Microorganisms.
It requires three successive steps: a selective enrichment, an isolation on a chromogenic agar, and if positive a confirmation with a serological test (and if required, a selective media).

This methodology has been developed to determine enterococci in biostimulants as a single microorganism component or in a mixture with other microorganisms. This document is not applicable to mineral fertilizers that are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Council Directive 79/373/EEC) [3].

This document specifies the procedure for the determination and calculation of the dry matter fraction of plant biostimulants for which the results of performed analysis are to be calculated to the dry matter basis.
This document is applicable to the fertilizing product blends belonging to PFC 7 where the EU fertilising product plant biostimulant contained in the blend represents the highest % by mass in the blend. In case of equal shares, the user can apply either this or the standard(s) applicable to the other component product(s).

This document specifies the terms and definitions referred to in the plant biostimulant field and consists of 6 subclauses:
3.1   Claims
3.2   Terms relating to components
3.3   Terms relating to application method
3.4   Terms relating to sampling
3.5   Terms relating to physical form
3.6   Others terms relating to plant biostimulants

This document provides a method for the detection of Listeria monocytogenes in microbial plant biostimulants for verifying that the content of this human pathogen agrees with the respective limits outlined in the EU Regulation on Fertilising Products [1].

This document specifies the general principles for justifying the product claims for plant biostimulants. It is applicable to all application types of plant biostimulants.
General principles consist of and define all general parameters, requirements and quality criteria, and are intended to be applied in order to assess the efficacy of trials used for claim(s) validation as a result of the use of a plant biostimulant.
This document is aimed primarily at manufacturers, laboratories, researchers, technical centres, companies that will put the products on the market, notifying authorities, notified bodies, and market surveillance authorities.

This document specifies the methods to be used for the determination of quantity of solid and liquid forms of plant biostimulants in packages, containers or in bulk.
This document is not applicable to the quantity determination of: soil improvers, growing media, organic and organo-mineral fertilizers and fertilizing product blends whose main constituent is a growing media or soil improver. The method for quantity determination for these products is given in EN 15761, EN 15238 and EN 12580.

This document specifies sampling plans and methods of representative sampling of plant biostimulants to obtain samples for physical, chemical and biological analysis.
It is applicable to the sampling of batches of plant biostimulants supplied or ready for supply to third parties, as such, or in smaller batches.
It is also applicable to the sampling of blends of fertilizing products where plant biostimulants are main part of the blend. Otherwise, deliverables of sampling relevant for the main part of the blend apply.
This document is intended to be used by manufacturers, buyers and competent authorities to obtain samples prior to transport and supply it to a laboratory for testing.
NOTE   This document is applicable to the category of EU fertilizing product (plant biostimulants) in the meaning of Regulation (EU) 2019/1009.

This document was developed to provide the methodology for the enumeration and determination of Azotobacter sp. [2] [3] in microbial plant biostimulants in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

This document provides the methodology for the enumeration and determination of Rhizobium sp., Mesorhizobium sp., Ensifer sp., or Bradyrhizobium sp. in plant biostimulant products in accordance with Regulation (EU) 2019/1009 of the European Parliament and of the Council [1].

This document provides guidance for justifying agronomic nutrient use efficiency claims of plant biostimulants used in agriculture.
To be in compliance with this standard, it is important also to follow the Recommendations and Quality Criteria described in the Standard of General Principles EN 17700 1:—1.
This document is aimed primarily at manufacturers, laboratories, researchers, technical centres, companies that will put the products on the market, notifying authorities, notified bodies, and market surveillance authorities.

This document specifies a method for the determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in aqua regia plant biostimulant digests using inductively coupled plasma-atomic emission spectrometry (ICP-AES).
This document is applicable to the fertilizing product blends belonging to PFC 7 where the EU fertilising product plant biostimulant contained in the blend represents the highest % by mass in the blend. In case of equal shares, the user can apply either this or the standard(s) applicable to the other component product(s).
This method is applicable to aqua regia digests prepared according to EN 17701 1:—1. The method can be used for the determination of other elements, provided the user has verified the applicability.
NOTE   Alternatively, inductively coupled plasma mass spectrometry (ICP-MS) can be used for the determination of the elements in the aqua regia digests if the user proves that the method gives the same results.

This standard was developed to provide a horizontal method for enumeration and genera/species determination [1], [2], [3] of mycorrhizal fungi in microbial plant biostimulant in accordance with the Regulation (EU) 2019/1009 of the European Parliament and of the Council.

This document provides a method for verifying that the pathogen Staphylococcus aureus is absent from microbial plant biostimulants according to the limits outlined in the EU Regulation on Fertilising Products [2].

This document specifies the method for the digestion of different plant biostimulants with aqua regia to enable a subsequent determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), mercury (Hg), nickel (Ni), lead (Pb) and zinc (Zn). The method can be also applied for determination of other elements. The method is applicable for all solid and/or liquid plant biostimulants.
This document is also applicable to the fertilizing product blends belonging to PFC 7 where the EU fertilising product plant biostimulant contained in the blend represents the highest % by mass in the blend. In case of equal shares, the user can apply either this or the standard(s) applicable to the other component product(s).
The extracts are suitable for analysis using EN 17701 2:—  (ICP-AES) and EN 17701 3:—  (Hg analysis).
NOTE   Alternatively, inductively coupled plasma mass spectrometry (ICP-MS) can be used for the determination of the elements in the aqua regia digests if the user proves that the method gives the same results.

This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract [1]. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the following:
-   microbial plant biostimulants.
NOTE 1   The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [1].
NOTE 2   For confirmation, it is possible to use PCR tests; in this case the laboratory must validate the procedure and data generated.

This document defines general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examinations of microbial plant biostimulants.
This horizontal method might not be appropriate in very detail for certain products. In this case, different methods which are specific to these products can be used if necessary, for justified technical reasons.

The claim described in this document concerns the improvement of availability of confined nutrients in the soil or rhizosphere by a plant biostimulant.
This document is aimed primarily at manufacturers, laboratories, researchers, technical centres, companies that will put the products on the market, notifying authorities, notified bodies, and market surveillance authorities.
To be in compliance with this standard, it is important also to follow the Recommendations and Quality Criteria described in the Standard of General Principles EN 17700 1:—1.

This document specifies a method for the extraction and determination of phosphonates (P-PO3) in plant biostimulants using ion chromatography and conductivity detection (IC-CD).
This document is applicable to the fertilizing product blends belonging to PFC 7 where the EU fertilising product plant biostimulant contained in the blend represents the highest % by mass in the blend. In case of equal shares, the user may apply either this or the standard(s) applicable to the other component product(s).

This document specifies methods for the reduction and preparation of samples of non-microbial plant biostimulants including those intended for determination of microbial pathogens and sets out the requirements for sample preparation reports. It specifies methods for the preparation of test samples and test portions from laboratory samples of plant biostimulants for subsequent chemical, biological or physical analysis.
It is also applicable to the sample preparation of blends of fertilizing products where plant biostimulants are main part of the blend. Otherwise, deliverables of sample preparation relevant for the main part of the blend apply.
This document does not include methods for the reduction and preparation of samples of microbial plant biostimulants, which will be covered by a different European Standard.
NOTE   This document is applicable to the category of EU fertilizing product (plant biostimulants) in the meaning of Regulation (EU) 2019/1009.

This document specifies a method for extraction, separation, and determination of inorganic arsenic (iAs) in plant biostimulants using anion-exchange high performance liquid chromatography (HPLC) or ion chromatography (IC) coupled to ICP-MS. This document is applicable to the fertilizing product blends belonging to PFC 7 where the EU fertilising product plant biostimulant contained in the blend represents the highest % by mass in the blend. In case of equal shares, the user can apply either this or the standard(s) applicable to the other component product(s).

This document was developed to provide a method for verifying that hexavalent chromium (CrVI) is not present in plant biostimulants in a concentration that exceeds the respective limits outlined in the EU Regulation on Fertilising Products [1].
This document is applicable to all types of plant biostimulants (solid and liquid ) used in agriculture.
The method described is suitable to quantify the chromium(VI) content in plant biostimulants down to 2 mg/kg.
The results obtained from this method are strictly dependent on the extraction conditions. Results obtained by using other extraction procedures (extraction solution, pH, extraction time, etc.) are not comparable with the results produced by the procedure described in this document. Therefore, this document is highly recommended to quantify the chromium(VI) content in blends of fertilizing products containing plant biostimulants, independently of the percentage of the different parts forming the blend.

This document specifies the method for taking a sample of solid and liquid forms of organic fertilizers, organo-mineral fertilizers and inorganic fertilizers containing more than 1 % by mass of organic carbon, when in packages, containers or in bulk, to test for levels of controlled pathogens present.

This document specifies methods for determining temperature-time profiles during composting and anaerobic digestion for the production of compost and digestate.
The process monitoring is an organized check and recording of the temperature during a specific time of the composting and anaerobic digestion process.
This document only applies to composting and anaerobic digestion.
This document is intended to be used by manufacturers and enforcement agencies for the purpose of manufacturing control.
The requirements of this document can differ from national legal requirements for the production process of compost and digestate.

This document specifies methods for the reduction and preparation of samples of fertilizers, liming materials, inhibitors and blends and sets out the requirements for sample preparation reports. It also specifies methods for the preparation of test samples and test portions from laboratory samples of fertilizer for subsequent chemical or physical analysis. It does not cover the preparation of samples for certain physical tests which require test portions of more than 2 kg.

This document is applicable to the sampling of the following solid inorganic fertilizers and liming materials supplied or ready for supply, and stored in static heaps:
- Single nutrient fertilizers,
- Uniform complex fertilizers,
- Milled, granulated or dredged liming materials,
- Any other materials deemed suitable for sampling by the method described in this part of the standard by any other standard,
for the purpose of testing for compliance with legal requirements and labelled descriptions and declarations.

This European Standard specifies sampling plans and methods of representative sampling of non organic and non organo-mineral fertilizers, liming materials and inhibitors to obtain samples for physical and chemical analysis, from packages and containers up to and including 1 000 kg, from liquid products and from fertilizers in bulk provided the product is in motion. It is applicable to the sampling of lots of fertilizer or liming material supplied or ready for supply to third parties, as such, or in smaller lots, each of which would be subject to local, national or regional legislation. Where legislation so requires, samples are taken in accordance with this European Standard. This European Standard does not cover complete, statistical sampling plans.