This document specifies a method for the determination of total nitrogen in nitrate-free calcium cyanamide.

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This document specifies a method for the determination of total nitrogen in calcium cyanamide. The method is applicable to calcium cyanamide containing nitrates.

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This document specifies a method for the determination of cyanamide nitrogen in fertilizers. The method is applicable to calcium cyanamide and mixtures of calcium cyanamide with calcium nitrate.

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This document specifies an inductively coupled plasma-optical emission spectrometer (ICP-OES) method for the determination of the ammonium citrate, disodium-etheylenediamine tetraacetic acid (EDTA) soluble phosphorus and potassium content in fertilizer extract solutions. This document is applicable to all inorganic fertilizer products, whether compound or complex fertilizer products or blends thereof, and straight concentrated fertilizer products.

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TThis document specifies a complexometric method for the determination of the calcium content and the magnesium content of liming materials.
It is not applicable to products with a mass fraction less than 2 % magnesium or those with a mass fraction more than 1 % P2O5 and is not applicable to silicate liming materials.

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This document is applicable to liming materials, which contain oxides, hydroxides, carbonates or silicates of the nutrients calcium (Ca) or magnesium (Mg) and the function of which is to correct soil acidity. It is applicable to fertilizing products, which are classified as PFC 2 or the PFC 2 component in PFC 7 of Regulation (EU) 2019/1009 [3].
NOTE   The methods referred to in this document have not been tested for fertilizing product blends (PFC 7) [3].
This document specifies references to the methods for the determination of the following physical and chemical properties and specific contaminants in liming materials:
-   Determination of the cadmium content;
-   Determination of the chromium VI content;
-   Determination of the mercury content;
-   Determination of the nickel content;
-   Determination of the lead content;
-   Determination of the arsenic content;
-   Determination of the total chromium content;
-   Determination of neutralizing value;
-   Determination of the reactivity;
-   Determination of the grain size/granulometry;
-   Determination of the total CaO content;
-   Determination of the total MgO content.
This document does not specify references to the methods for the determination of the following physical and chemical properties and specific contaminants in liming materials, as they are covered in other European Standards:
-   Determination of the copper and zinc content;
-   Determination of the chloride content;
-   Determination of quantity (indicated by mass or volume);
-   Determination of the phosphonates content.

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This document specifies general requirements, analytical methods, sampling and preparation of test sample, marking and labelling, packaging, transport and storage for liquid methylene-urea slow release fertilizers. This document applies to pure liquid slow release fertilizers, i.e. methylene urea (MU). This document does not apply to mixtures of nitrogenous fertilizers containing liquid slow release fertilizers.

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This European Standard specifies a method for the determination of quantity of soil improvers, excluding liming materials and growing media in bulk and in packages. This document applies to material that is in solid form, but not in block form to be sold by dimension, and which exceeds the particle size restriction in EN 12580 and where the declared nominal particle size is greater than 60 mm.

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This European Standard specifies methods for the determination of a quantity of soil improvers and growing media in bulk and in packages. This is a reference method, which is designed with an appropriate precision level so that it can be used to validate any quantity declaration made.
This standard is applicable to material that is in solid form, reconstituted if necessary, but not to blocks sold as such by dimension; for these, see EN 15761. This method is not applicable for material with more than 10 % (V/V) of particles greater than 60 mm in size; for these, see EN 15238.
The requirements of this standard may differ from the national legal requirements for the declaration of the products concerned.
Where there is no legal requirement to use this method, for example in quantity control of packaged product, then it is permissible for any other methods to be used so long as these other methods can be demonstrated to be comparable with this standard method in giving the same quantity with the same precision.
Material which has become excessively wet and which cannot be easily broken down into a flowable material will not be suitable for the determination of quantity and may not give a representative result. However, because of the diverse nature and bulk density of these materials, it is not possible to quantify what is ‘excessive’.
This standard is intended to be used by manufacturers, buyers and enforcement agencies in verifying claims made for these products. It is not intended that it should necessarily be used for the purpose of manufacturing control.

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This document specifies three different methods (Methods A, B and C) for the determination of sulfur present in fertilizers extracts in the form of sulfates. Method A specifies the gravimetric method. Method B specifies the method using inductively coupled plasma optical spectrometry (ICP-OES). Method C specifies the method using ion chromatography (IC).

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This document specifies a method for the determination of the chloride content in organic fertilizers, organo-mineral fertilizers, inorganic fertilizers and liming materials by potentiometric titration.

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This document specifies the procedure for the determination and calculation of the dry matter fraction of plant biostimulants for which the results of performed analysis are to be calculated to the dry matter basis.
This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend apply.

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The document establishes the methodology for the extraction of phosphorus, which is soluble in neutral ammonium citrate in organo-mineral fertilizers.

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The document establishes the methodology for the determination of the inorganic arsenic content.

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The document establishes the methodology for the determination of the total content of P, K, Ca, Mg, Na, S, B, Co, Cu, Fe, Mn, Mo, Zn, As, Cd, Cr, Ni, Pb by ICP-AES after their digestion by aqua regia.

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This document gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in technical and formulated biostimulant products, both in liquid and solid state, and also the horizontal method for the enumeration of ß-glucuronidase-positive Escherichia coli in plant biostimulants products (both in liquid and solid state).
The qualitative method described in this document is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods can be appropriate, depending on the level of detection required.
NOTE   For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).
The quantitative method described in this document uses a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme ß-glucuronidase.
WARNING - Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are ß-glucuronidase negative, such as Escherichia coli O157, will not be detected.

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This document specifies a method for the determination of the dry matter content in organic and organo-mineral fertilizers.

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This document specifies a method for the determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), lead (Pb), nickel (Ni) and zinc (Zn) in plant biostimulant digests using inductively coupled plasma-atomic emission spectrometry (ICP-AES).
This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend applies.
This method is applicable to aqua regia digests prepared according to CEN/TS 17701-1. The method can be used for the determination of other elements, provided the user has verified the applicability.

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This document establishes the methodology for the extraction of phosphorus by formic acid for subsequent determination of P in organo-mineral fertilizers.

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This document specifies a method for the determination of the chelated fraction of micronutrients for fertilizers when one or many micronutrients are chelated by EDTA, DTPA, HEEDTA, IDHA or [S,S]-EDDS in fertilizers.
This method is used for inorganic micronutrient fertilizers when micronutrients are chelated only by EDTA, DTPA, HEEDTA, IDHA or [S,S]-EDDS or for mixtures in which EDTA, DTPA, HEEDTA, IDHA or [S,S]-EDDS is one of the chelating agents.
The method is applicable to all inorganic micronutrient fertilizers containing EDTA, DTPA, HEEDTA, IDHA or [S,S]-EDDS as chelating agent for contents > 0,1% (g/100 g).
The method is based on ICP or AAS measurement of the concentration of micronutrients according to EN 16963 or EN 16965 after water extraction according to EN 16962 and LC measurement of the chelating agents according to EN 15950, EN 13368-1 and EN 13368-3.

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This document specifies a method for the determination by ion chromatography of the total amount of each of the individual chelating agents EDTA, HEEDTA, and DTPA in organo-mineral fertilizers, having an organic matrix based on vegetal residues (cocoa shells, grape residue, soybean residue, …), algae extract, and animal meal (feather, bones, blood, ...) and containing one or more of these substances.
The method allows the identification and the determination of the total water-soluble fraction of each of these chelating agents. It does not allow to distinguish between the free form and the metal bound form of the chelating agents.
This method applies to organo-mineral fertilizers containing chelates of one or more of the following micronutrients: cobalt, copper, iron, manganese, zinc and with a mass fraction of at least 0,1 %.

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The document establishes the methodology for the digestion by aqua regia of the different nutrients and trace elements (P, K, Ca, Mg, Na, S, B, Cu, Co, Fe, Mn, Mo, Zn, As, Cd, Cr, Hg, Ni, Pb) before the determination of their total content.

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The document establishes the methodology for the determination of :
- total P2O5, K2O, CaO, MgO, Na2O, SO3 content in organic and organo-mineral fertilizers;
- inorganic As, Cd, total Cr, Hg, Ni, Pb, Cu, Zn content in organic and organo-mineral fertilizers;  
- water soluble CaO, MgO, Na2O, SO3 content in organic and organo-mineral fertilizers;
- water soluble P2O5, K2O content in organo-mineral fertilizers;
- neutral ammonium citrate soluble P2O5 content in organo-mineral fertilizers;
- formic acid soluble P2O5 content in organo-mineral fertilizers;
- total B, Co, Fe, Mn, Mo content in organo-mineral fertilizers;
- water soluble B, Co, Cu, Fe, Mn, Mo, and Zn content in organo-mineral fertilizers;

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This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009 [1]. However, the present method was not validated for blends.
This document specifies a method for the detection of Salmonella spp. in organic, organo-mineral and inorganic fertilizers. The method is based on EN ISO 6579-1 and its validated alternative methods for the detection of Salmonella spp. in food and feeding stuff.
It requires three successive steps: A selective enrichment, an isolation on a chromogenic agar, and if positive a confirmation with a serological test (and if required, a selective media).

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This document specifies a method for the determination of the fraction of chelated and complexed micronutrients in compound inorganic micronutrient fertilizers.

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This document specifies a chromatographic method which allows the identification of heptagluconic acid (HGA) in organo-mineral fertilizers containing heptagluconic acid metal complexes.
NOTE   For the complete names of the chelating agents mentioned in this document, see Annex D.

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This document specifies a method for the determination of the chelated micronutrient content and the chelated fraction of a micronutrient, in organo-mineral fertilizers, having an organic matrix based on vegetal residues (cocoa shells, grape residue, soybean residue, etc), algae extract, and animal meal (feather, bones, blood, etc) and containing UVCB, EDDHA, EDDHMA, HBED, EDDHSA micronutrients by the treatment with a cation exchange resin.
The limit of determination of the chelated micronutrient content highly depends on the specific electrical conductivity of the sample, on the amount of nutrient present, and varies between 0,005 % in simple matrices with high amounts of micronutrient, and 0,5 % in more complex cases (see 9.1).

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This document specifies the procedure for the digestion of different plant biostimulants with aqua regia to enable a subsequent determination of arsenic (As), cadmium (Cd), copper (Cu), chromium (Cr), mercury (Hg), nickel (Ni), lead (Pb) and zinc (Zn). The procedure can be also applied for determination of other elements. The procedure is applicable for all solid and/or liquid plant biostimulants.
This document is also applicable to the blends of fertilizing products where plant biostimulants are the main part of the blend. Otherwise, the Technical Specification for the main part of the blend applies.
The extracts are suitable for analysis using CEN/TS 17701-2 (ICP-AES) and CEN/TS 17701-3 (Hg analysis).
NOTE   Alternatively, inductively coupled plasma mass spectrometry (ICP-MS) can be used for the measurement if the user proves that the method gives the same results.

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The document establishes the methodology for the determination of the mercury content.

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This document specifies a method for the determination by ion pair chromatography of the iron chelated by each individual ortho(hydroxy)-ortho(hydroxy) isomer of the chelating agents [o,o] EDDHA, [o,o] EDDHMA and by HBED in organo-mineral fertilizers, having an organic matrix based on vegetal residues (cocoa shells, grape residue, soybean residue, …), algae extract, and animal meal (feather, bones, blood, ...) and containing one or more of these substances, except for [o,o] EDDHMA and HBED mixes.
The method allows the identification and the determination of the total concentration of water soluble iron chelates of these chelating agents. Also, after derivatization with Fe, the soluble amount of the chelating agents can be determined when other micronutrients beside Fe are present in organo-mineral fertilizers containing [o,o] EDDHA, [o,o] EDDHMA or HBED.
This method is applicable to a mass fraction of the metal chelated of at least 0,625 %.
NOTE 1   The substances EDDHA and EDDHMA exist as several different isomeric forms. Positional isomers for the hydroxyl or methyl groups (in ortho, meta, and para positions) as well as stereo isomers (meso and dl-racemic forms) are known. Both meso and dl-racemic forms of the [ortho,ortho] EDDHA and [ortho,ortho]. Since para, meta and ortho methyl positional isomers of the EDDHMA present quite similar stability, they could be grouped: in the method here described the para, meta and ortho methyl positional isomers of the [o,o] EDDHMA are considered together. HBED (N,N'-bis(2-hydroxybenzyl)-ethylenediamine-N,N'-diacetic acid) does not present isomeric forms.
NOTE 2   At present, analytically pure standards only exist for [ortho,ortho] EDDHA, [ortho,ortho] EDDHMA and HBED. All other substances being unavailable as a standard, the influence of their eventual presence in the samples (with respect to the sensitivity and the selectivity of this method) has not been studied.
NOTE 3   The meso and the dl-racemic forms of [o,o] EDDHA and [o,o] EDDHMA can be determined separately by this method.

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This document establishes the methodology for the determination of the biuret content.

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This document specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract [1]. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the following:
-   microbial plant biostimulants.
NOTE 1   The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [1].
NOTE 2   For confirmation, it is possible to use PCR tests; in this case the laboratory validates the procedure and data generated.

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This document specifies references to the methods for the determination of chelating and complexing agents in organo-mineral fertilizers. The document specifies references to the methods and requirements for organo-mineral fertilizers in accordance with PFC 1 (B) as specified in the Regulation (EU) 2019/1009 [1].
Organo-mineral materials for this purpose are organic fertilizers containing micronutrient chelates or complexes and/or mixtures of them, in powder or granular form, aqueous or suspension preparations.

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The document establishes the methodology for the determination of the chromium VI content in organic and organo-mineral fertilizers by ion chromatography.

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This document specifies two methods required for the identification of lignosulfonate by UV-Vis spectrophotometry (method A) and gravimetry (method B) in organo-mineral fertilizers.
NOTE   Lignosulfonate, as a complexing agent, is a natural polymer produced as a by-product of the sulfite method for manufacturing paper from wood pulp in the paper industry. As a natural polymer, it presents a poorly defined and variable chemical structure. It is an intricate mixture of small- to moderate-sized polymeric compounds with sulfonate groups attached to the molecule, and diverse complexing capacity.

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The document establishes the methodology for the determination of the nitrogen content including total nitrogen and ammoniacal, nitric, urea, organic nitrogen as well as the calculation of the nitrogen content as a result of ammonium nitrate.

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This document is applicable to fertilizing products, which are classified as PFC 1(A) and PFC 1(B) or the PFC 1(A) and PFC 1(B) component in PFC 7 of Regulation (EU) 2019/1009 [1]. However, the present method was not validated for blends.
This document specifies a colony-count technique at 44 °C on a solid medium containing a chromogenic ingredient for the detection of the enzyme ß-glucuronidase. The method is based on ISO 16649-2 [4].
Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are ß-glucuronidase negative, such as Escherichia coli O157, will not be detected. Detected microorganisms are presumptively determined β-glucuronidase-positive Escherichia coli, since some Enterobacteriaceae, in particular Shigella and Salmonella, can also show β-glucuronidase activity at 44 °C.

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This document specifies a method for the determination of pH of a solution of ammonium nitrate fertilizers of high nitrogen content.

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The document establishes the methodology for the determination of specific parameters including the determination of the organic carbon content and the dry matter content.

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This document specifies a method for the determination of the stability of micronutrient complexes.

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This document provides the methodology for the enumeration and determination of Azospirillum sp. in plant biostimulant products in accordance to the Regulation of EU fertilising products [1].

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The document establishes the methodology for the determination of water-soluble content of P, K, Ca, Mg, Na, S, B, Co, Cu, Fe, Mn, Mo, Zn by ICP-AES.

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This document specifies the references to the methods for the determination of stability of chelating and complexing agents for CMC 1 as specified in the Regulation (EU) 2019/1009. The document specifies references to the methods and requirements for inorganic micronutrient fertilizers in accordance with PFC 1(C)(II) as specified in the Regulation (EU) 2019/1009 [1].
Inorganic micronutrient materials for this purpose are micronutrient chelates or complexes and mixtures of them, in powder or granular form, aqueous or suspension preparations.

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The document establishes the methodology for the extraction by water of different nutrients : P, K, Ca, Mg, Na, S, B, Co, Cu, Fe, Mn, Mo, Zn before the determination of their water-soluble content.

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This document specifies a method for the determination of the soluble metal that remains in solution at different pHs after the application of a solution of the fertilizer substance containing micronutrient chelates in a tap water solution used as a reference.
The method applies to fertilizing products containing chelated micronutrients.

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This document specifies a method for the determination of the chelated iron content and the chelated fraction of iron, in UVCB, EDDHA, EDDHMA, HBED, EDDHSA, in inorganic micronutrient fertilizers by the treatment with a cation exchange resin.
The limit of determination of the chelated iron content highly depends on the specific electrical conductivity of the sample, on the amount of nutrient present, and varies between 0,005 % in simple matrices with high amounts of micronutrient and 0,5 % in more complex cases (see 9.1).

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This document specifies a horizontal method for the enumeration of yeasts and moulds present in plant biostimulant intended for use in agriculture, by means of the colony count technique after aerobic incubation at 25 °C +- 2,5 °C.
This document allows the enumeration of yeasts and moulds, in technical and formulated plant biostimulant, both in liquid and solid state. The method is applicable to microbial plant biostimulant except those composed of fungi or yeast to verify that the concentration of yeast and moulds does not exceed the respective limits described in the EU Fertilizers Regulation [1].
If necessary, yeast and mould enumerated can be identified using suitable identification tests.

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This document specifies a general method for the determination of the micronutrients complexed by complexing agents in organo-mineral fertilizers. The method allows the determination of the total concentration of each complexed micronutrient in complexes after subtraction of the chelated micro-nutrients content, but it does not identify the individual complexing agents.
This procedure concerns EU organo-mineral fertilizing products which contain complexed micro-nutrients covered by Regulation (EU) 2019/1009 [6]. The method is applicable to a mass fraction of the metal complexed of at least 0,07 %, 0,006 % and 0,035 % of Fe, Mn and Zn respectively (see [7]). A lower limit of quantification has not been established for Cu and Co.

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This document specifies references to the methods for the determination of specific micronutrients, chelating and complexing agents. The document specifies references to the methods and requirements for inorganic micronutrient fertilizers in accordance with PFC 1 (C) (II) as specified in the Regulation (EU) 2019/1009 [1].
Inorganic micronutrient materials for this purpose are micronutrient salts or oxide and hydroxides, or micronutrient chelates or complexes and mixtures of them, in powder or granular form, aqueous or suspension preparation.

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This document specifies a method for the determination of the copper content in ammonium nitrate fertilizers of high nitrogen content.

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