Footwear and footwear components - Test method to assess antibacterial activity (ISO/DIS 16187:2023)

This document specifies quantitative test methods to evaluate the antibacterial activity of footwear and components.
This document is applicable to all types of footwear and components employing non-diffusing antibacterial treatments.

Schuhe und Schuhbestandteile - Prüfverfahren zur Bestimmung der antibakteriellen Wirkung (ISO/DIS 16187:2023)

Chaussure et composants de chaussure - Méthode d'essai pour évaluer l'activité antibactérienne (ISO/DIS 16187:2023)

Obutev in deli obutve - Preskusna metoda za ugotavljanje protibakterijskega delovanja (ISO/DIS 16187:2023)

General Information

Status
Not Published
Publication Date
13-Jul-2025
Technical Committee
Drafting Committee
Current Stage
4599 - Dispatch of FV draft to CMC - Finalization for Vote
Start Date
07-Nov-2024
Completion Date
07-Nov-2024

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SLOVENSKI STANDARD
01-oktober-2023
Obutev in deli obutve - Preskusna metoda za ugotavljanje protibakterijskega
delovanja (ISO/DIS 16187:2023)
Footwear and footwear components - Test method to assess antibacterial activity
(ISO/DIS 16187:2023)
Schuhe und Schuhbestandteile - Prüfverfahren zur Bestimmung der antibakteriellen
Wirkung (ISO/DIS 16187:2023)
Chaussure et composants de chaussure - Méthode d'essai pour évaluer l'activité
antibactérienne (ISO/DIS 16187:2023)
Ta slovenski standard je istoveten z: prEN ISO 16187
ICS:
61.060 Obuvala Footwear
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT INTERNATIONAL STANDARD
ISO/DIS 16187
ISO/TC 216 Secretariat: UNE
Voting begins on: Voting terminates on:
2023-08-04 2023-10-27
Footwear and footwear components — Test method to
assess antibacterial activity
Chaussure et composants de chaussure — Méthode d'essai pour évaluer l'activité antibactérienne
ICS: 61.060
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 16187:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

ISO/DIS 16187:2023(E)
DRAFT INTERNATIONAL STANDARD
ISO/DIS 16187
ISO/TC 216 Secretariat: UNE
Voting begins on: Voting terminates on:

Footwear and footwear components — Test method to
assess antibacterial activity
Chaussure et composants de chaussure — Méthode d'essai pour évaluer l'activité antibactérienne
ICS: 61.060
This document is circulated as received from the committee secretariat.
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENT AND APPROVAL. IT IS
© ISO 2023
ISO/CEN PARALLEL PROCESSING
THEREFORE SUBJECT TO CHANGE AND MAY
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
NOT BE REFERRED TO AS AN INTERNATIONAL
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on STANDARD UNTIL PUBLISHED AS SUCH.
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
IN ADDITION TO THEIR EVALUATION AS
or ISO’s member body in the country of the requester. BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
ISO copyright office
USER PURPOSES, DRAFT INTERNATIONAL
CP 401 • Ch. de Blandonnet 8
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
CH-1214 Vernier, Geneva
POTENTIAL TO BECOME STANDARDS TO
Phone: +41 22 749 01 11
WHICH REFERENCE MAY BE MADE IN
Reference number
Email: copyright@iso.org
NATIONAL REGULATIONS.
Website: www.iso.org ISO/DIS 16187:2023(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
Published in Switzerland
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
ii
PROVIDE SUPPORTING DOCUMENTATION. © ISO 2023

ISO/DIS 16187:2023(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Safety . 2
6 Apparatus and materials .2
7 Reagents and culture medium .3
7.1 General . 3
7.2 Water . 3
7.3 Nutrient broth (NB) . 3
7.3.1 Composition . 3
7.3.2 Preparation . 3
7.4 Nutrient agar (NA) . 3
7.4.1 Composition . 3
7.4.2 Preparation . 4
7.5 Tryptic Soy Broth (TSB) . 4
7.5.1 Composition . 4
7.5.2 Preparation . 4
7.6 Tryptone soy agar (TSA) . 4
7.6.1 Composition . 4
7.6.2 Preparation . 4
7.7 Soybean casein digest broth with lecithin and polyoxyethylene medium (SCDLP) . 5
7.7.1 Composition . 5
7.7.2 Preparation . 5
7.8 Sodium chloride solution (physiological saline). 5
7.8.1 Composition . 5
7.8.2 Preparation . 5
8 Test microorganisms . 5
8.1 Test strains. 5
8.2 Storage of strains . 6
9 Preparation of test inoculums .6
10 Preparation of test samples .6
10.1 General . 6
10.2 Test specimen . 6
10.3 Pre-treatment of the test specimen . 7
11 Test procedure .7
12 Expression of results .7
12.1 Calculation of the number of viable bacteria . 7
12.2 Judgement of test effectiveness . 8
12.3 Calculation of antibacterial activity ratio . 8
13 Test report . 9
Annex A (normative) Static challenge test .10
Annex B (normative) Film contact method .11
Annex C (normative) Dynamic challenge test.13
Bibliography .14
iii
ISO/DIS 16187:2023(E)
Foreword
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constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions rela
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