EN ISO 10519:2015/A1:2025

Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment 1: Preparation of the calibration curve to determine the k factor (ISO 10519:2015/Amd 1:2025)

Rapssamen - Bestimmung des Chlorophyllgehaltes - Spektrometrisches Verfahren - Änderung 1: Erstellung der Kalibrierkurve zur Bestimmung des k-Faktors (ISO 10519:2015/Amd 1:2025)

Graines de colza - Détermination de la teneur en chlorophylle - Méthode spectrométrique - Amendement 1: Préparation de la courbe d’étalonnage pour déterminer le facteur k (ISO 10519:2015/Amd 1:2025)

Seme oljne repice - Določevanje vsebnosti klorofila - Spektrometrijska metoda - Dopolnilo A1: Priprava kalibracijske krivulje za določitev k-faktorja (ISO 10519:2015/Amd 1:2025)

General Information

• Status: Published
• Publication Date: 22-Apr-2025
• ICS: 67.200.20 - Oilseeds
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 23-Apr-2025
• Completion Date: 23-Apr-2025
• Ref Project: SIST EN ISO 10519:2015/A1:2025 - Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment 1: Preparation of the calibration curve to determine the k factor (ISO 10519:2015/Amd 1:2025)

Relations

• Consolidated By: ISO 10519:2015/Amd 1:2025 - Rapeseed — Determination of chlorophyll content — Spectrometric method — Amendment 1: Preparation of the calibration curve to determine the k factor
• Effective Date: 12-Feb-2026
• Amends: EN ISO 10519:2015 - Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO 10519:2015)
• Effective Date: 21-Jun-2023

Overview

EN ISO 10519:2015/A1:2025 is an amendment to the international standard for the determination of chlorophyll content in rapeseed using a spectrometric method. This update, published by the European Committee for Standardization (CEN), specifically adds a procedure for the preparation of the calibration curve to determine the k factor, as required in accurate chlorophyll analyses. The k factor is a crucial constant that depends on the path length and bandwidth of the spectrophotometer, and its correct determination ensures precision and comparability in measurement results.

Key Topics

• Chlorophyll Content in Rapeseed: Establishes a standardized approach to assess chlorophyll levels, which is significant for oilseed quality and processing.
• Spectrometric Analysis: Utilizes absorption measurements between 600 nm and 700 nm, emphasizing the impact of spectral bandwidth and instrument calibration.
• Calibration Curve Preparation: Details the method for preparing a calibration curve using standard chlorophyll A solutions, enabling laboratories to determine the specific k factor for their spectrometers.
• Reagents and Apparatus: Specifies the use of high-purity reagents-such as diethyl ether and chlorophyll A-and precise laboratory equipment including volumetric flasks, micropipettes, and spectrometers with known bandwidth.
• Quality Assurance: Highlights the necessity to set and maintain constant bandwidth for accurate, repeatable results and underscores the relationship between instrument characteristics and analysis validity.

Applications

Implementing EN ISO 10519:2015/A1:2025 brings substantial benefits to a range of sectors involved in rapeseed handling and processing:

• Quality Control in Oilseed Processing: Chlorophyll content can affect the taste, color, and shelf-life of rapeseed oil. Reliable measurement by standardized methods supports quality assurance and compliance with market and regulatory requirements.
• Laboratory Standardization: Laboratories engaged in agricultural and food product testing can ensure their spectrometric measurements are internationally comparable by calibrating their instruments using the described procedure.
• Research and Development: This standard empowers research institutions to generate robust data on rapeseed quality, supporting breeding programs, variety selection, and post-harvest technology improvements.
• Trade and Compliance: Meeting the requirements of EN ISO 10519:2015/A1:2025 can facilitate national and international trade by demonstrating adherence to recognized methods for assessing oilseed quality.

Related Standards

For users of EN ISO 10519:2015/A1:2025, the following standards and guidelines may also be relevant to ensure integrated quality management and comprehensive oilseed analysis:

• EN ISO 10519:2015 - The main standard for determination of chlorophyll content in rapeseed by spectrometric method.
• ISO 5508 / ISO 5509 - Methods for analysis of oilseeds and vegetable oils, which may be used alongside chlorophyll determination for broader quality assessment.
• CEN/TC 307 Standards - Covering oilseeds, vegetable oils, and derivatives, with a focus on sampling and analytical techniques.
• Good Laboratory Practice (GLP) - For reliable implementation of analytical standards in industry and research settings.

By following EN ISO 10519:2015/A1:2025, stakeholders across the oilseed supply chain, laboratories, and regulatory bodies can uphold the highest standards of analytical accuracy, supporting product quality and market confidence.

Standards Content (Sample)

SLOVENSKI STANDARD
01-junij-2025
Seme oljne repice - Določevanje vsebnosti klorofila - Spektrometrijska metoda -
Dopolnilo A1: Priprava kalibracijske krivulje za določitev k-faktorja (ISO
10519:2015/Amd 1:2025)
Rapeseed - Determination of chlorophyll content - Spectrometric method - Amendment
1: Preparation of the calibration curve to determine the k factor (ISO 10519:2015/Amd
1:2025)
Rapssamen - Bestimmung des Chlorophyllgehaltes - Spektrometrisches Verfahren -
Änderung 1: Erstellung der Kalibrierkurve zur Bestimmung des k-Faktors (ISO
10519:2015/Amd 1:2025)
Graines de colza - Détermination de la teneur en chlorophylle - Méthode
spectrométrique - Amendement 1: Préparation de la courbe d’étalonnage pour
déterminer le facteur k (ISO 10519:2015/Amd 1:2025)
Ta slovenski standard je istoveten z: EN ISO 10519:2015/A1:2025
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 10519:2015/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2025
EUROPÄISCHE NORM
ICS 67.200.20
English Version
Rapeseed - Determination of chlorophyll content -
Spectrometric method - Amendment 1: Preparation of the
calibration curve to determine the k factor (ISO
10519:2015/Amd 1:2025)
Graines de colza - Détermination de la teneur en Rapssamen - Bestimmung des Chlorophyllgehaltes -
chlorophylle - Méthode spectrométrique - Spektrometrisches Verfahren - Änderung 1: Erstellung
Amendement 1: Préparation de la courbe d'étalonnage der Kalibrierkurve zur Bestimmung des k-Faktors (ISO
pour déterminer le facteur k (ISO 10519:2015/Amd 10519:2015/Amd 1:2025)
1:2025)
This amendment A1 modifies the European Standard EN ISO 10519:2015; it was approved by CEN on 19 April 2025.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10519:2015/A1:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 10519:2015/A1:2025) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and
animal fats and oils and their by-products - Methods of sampling and analysis” the secretariat of which
is held by AFNOR.
This Amendment to the European Standard EN ISO 10519:2015 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by October 2025,
and conflicting national standards shall be withdrawn at the latest by October 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 10519:2015/Amd 1:2025 has been approved by CEN as EN ISO 10519:2015/A1:2025
without any modification.
International
Standard
ISO 10519
Third edition
Rapeseed — Determination
2015-09-15
of chlorophyll content —
Spectrometric method
AMENDMENT 1
2025-04
AMENDMENT 1: Preparation of the
calibration curve to determine the
k factor
Graines de colza — Détermination de la teneur en chlorophylle —
Méthode spectrométrique
AMENDEMENT 1: Préparation de la courbe d’étalonnage pour
déterminer le facteur k
Reference number
ISO 10519:2015/Amd.1:2025(en) © ISO 2025

ISO 10519:2015/Amd.1:2025(en)
© ISO 2025
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
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or ISO’s member body in the country of the requester.
ISO copyright office
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Published in Switzerland
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