EN 15457:2022
(Main)Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against fungi
Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in a coating against fungi
This document specifies a laboratory test method for determining the biocidal or biostatic efficacy of single active substances or combinations thereof used in film preservatives of coatings against fungal growth. This document does not apply to coatings unsusceptible to fungal growth. The test method covers only active substances for film preservation. It does not indicate the efficacy of the coating film to protect itself or an underlying material. The test method is applicable for active substances used for the protection of wood and masonry coatings. It is not applicable to marine coatings.
Safety, health and environmental aspects are not in the scope of this document.
Determination of the performance of film preservatives in coatings by applying ageing procedures is not within the scope of this document.
Beschichtungsstoffe - Laborverfahren für die Prüfung der Wirksamkeit von Filmkonservierungsmitteln in einer Beschichtung gegen Pilze
Dieses Dokument legt ein Laborverfahren zum Bestimmen der bioziden/biostatischen Wirksamkeit von Wirkstoffen oder einer Kombination aus mehreren Wirkstoffen zur Anwendung in Filmkonservierungsmitteln einer Beschichtung gegen Pilzbewuchs fest. Dieses Dokument gilt nicht für Beschichtungen, die unempfindlich gegen Pilzbewuchs sind. Das Prüfverfahren umfasst nur die Wirkstoffe zur Filmkonservierung. Es zeigt nicht an, wie wirksam der Beschichtungsfilm selbst oder ein Werkstoff darunter geschützt wird. Das Prüfverfahren ist anwendbar für Wirkstoffe, die für den Schutz von Beschichtungen von Holz und mineralischen Substraten verwendet werden. Die Norm gilt nicht für Beschichtungen im maritimen Bereich.
Sicherheits-, Gesundheits- und Umweltaspekte liegen nicht im Anwendungsbereich dieses Dokuments.
Die Bestimmung der Wirksamkeit von Filmkonservierungsmitteln in Beschichtungen nach Anwendung von Alterungsverfahren liegt nicht im Anwendungsbereich dieses Dokuments.
Peintures et vernis - Méthode d'essai en laboratoire permettant de déterminer l'efficacité des préservateurs du feuil d'un revêtement contre les champignons
Le présent document spécifie une méthode d’essai en laboratoire permettant de déterminer l’efficacité biocide ou biostatique de substances actives individuelles, ou de combinaisons de ces substances, utilisées dans les préservateurs du feuil de revêtements contre le développement fongique. Le présent document ne s’applique pas aux revêtements qui ne sont pas sensibles au développement fongique. Cette méthode d’essai concerne uniquement les substances actives utilisées pour la protection du feuil. Elle n’indique pas l’efficacité de protection du feuil de revêtement pour lui-même ou pour un matériau sous-jacent. Cette méthode d’essai est applicable aux substances actives utilisées pour la protection des peintures pour bois et pour maçonnerie. Elle ne s’applique pas aux peintures marines.
Barve in laki - Laboratorijske metode za preskušanje učinkovitosti konzervansov filma v premazih proti glivam
Ta dokument določa laboratorijsko preskusno metodo za določanje biocidne/biostatične učinkovitosti posamezne aktivne snovi ali kombinacij aktivnih snovi, ki se uporabljajo v konzervansih filma v premazih proti glivam. Dokument se ne uporablja za premaze, ki niso občutljivi na glive. Preskusna metoda vključuje samo aktivne snovi za ohranjanje filma in ne za zaščito same podlage, npr. lesa, ki jo obravnava drug standard. Preskusna metoda se uporablja za aktivne snovi, ki se uporabljajo za lesne in zidne premaze. Ne uporablja se za ladijske premaze.
Na področje uporabe tega dokumenta niso vključeni vidiki varnosti, zdravja in varstva okolja.
Določanje učinkovitosti konzervansov filma v premazih z izvajanjem postopkov staranja ne spada na področje uporabe tega dokumenta.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-september-2022
Nadomešča:
SIST EN 15457:2014
Barve in laki - Laboratorijske metode za preskušanje učinkovitosti konzervansov
filma v premazih proti glivam
Paints and varnishes - Laboratory method for testing the efficacy of film preservatives in
a coating against fungi
Beschichtungsstoffe - Laborverfahren für die Prüfung der Wirksamkeit von
Filmkonservierungsmitteln in einer Beschichtung gegen Pilze
Peintures et vernis - Méthode d'essai en laboratoire permettant de déterminer l'efficacité
des préservateurs du feuil d'un revêtement contre les champignons
Ta slovenski standard je istoveten z: EN 15457:2022
ICS:
87.040 Barve in laki Paints and varnishes
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN 15457
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2022
EUROPÄISCHE NORM
ICS 87.040 Supersedes EN 15457:2014
English Version
Paints and varnishes - Laboratory method for testing the
efficacy of film preservatives in a coating against fungi
Peintures et vernis - Méthode d'essai en laboratoire Beschichtungsstoffe - Laborverfahren für die Prüfung
permettant de déterminer l'efficacité des der Wirksamkeit von Filmkonservierungsmitteln in
préservateurs du feuil d'un revêtement contre les einer Beschichtung gegen Pilze
champignons
This European Standard was approved by CEN on 3 January 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15457:2022 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Apparatus and materials . 6
6 Fungi . 7
6.1 Fungi more likely to grow in an exterior environment . 7
6.2 Fungi more likely to grow in an interior environment . 7
7 Sampling and preparation of test samples and of test specimens . 7
7.1 Sampling . 7
7.2 Preparation of test samples (see Annex A) . 8
7.3 Conditioning of the test samples . 8
7.4 Preparation and number of test specimens . 8
8 Procedure. 8
8.1 Preparation of the Petri dishes with the culture medium . 8
8.2 Preparation of stock cultures and sub-cultures . 8
8.3 Preparation of the spore suspension . 8
8.4 Inoculation and incubation (see Annex A) . 9
8.5 Assessment . 9
9 Test report . 9
Annex A (informative) Laboratory method for testing the efficacy of film preservatives in a
coating against fungi . 11
Annex B (informative) Designation of the percentage area of disfigurements according to
EN 16492:2014, Table A.3 . 12
Bibliography . 13
European foreword
This document (EN 15457:2022) has been prepared by Technical Committee CEN/TC 139 “Paints and
varnishes”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2022, and conflicting national standards shall
be withdrawn at the latest by September 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN 15457:2014.
The main changes compared to EN 15457:2014 are as follows:
a) new terms and definitions have been added;
b) the use of terms and definitions throughout the document has been corrected;
c) the procedure in 8.4 has been corrected: the suspension is spread out onto the test specimen;
d) Figure A.1 has been corrected in accordance with corrected procedure in 8.4;
e) the document has been editorially revised and the normative references have been updated.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United
Kingdom.
Introduction
This document identifies criteria to assess efficacy of film preservatives in a coating against fungi. The
results of the method allow evaluation of an active substance with regard to its inclusion in Annex I of the
Biocidal Product Regulation 528/2012 (Regulation EU No 528/2012 of the European Parliament and of
the Council of 22 May 2012 concerning the placing of biocidal products on the market – BPR).
The characteristics of the biocide treated coating material should conform to national regulations with
regard to health, safety and the environment.
1 Scope
This document specifies a laboratory test method for determining the biocidal or biostatic efficacy of
single active substances or combinations thereof used in film preservatives of coatings against fungal
growth. This document does not apply to coatings unsusceptible to fungal growth. The test method covers
only active substances for film preservation. It does not indicate the efficacy of the coating film to protect
itself or an underlying material. The test method is applicable for active substances used for the
protection of wood and masonry coatings. It is not applicable to marine coatings.
Safety, health and environmental aspects are not in the scope of this document.
Determination of the performance of film preservatives in coatings by applying ageing procedures is not
within the scope of this document.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 12469, Biotechnology - Performance criteria for microbiological safety cabinets
EN 16492:2014, Paints and varnishes - Evaluation of the surface disfigurement caused by fungi and algae
on coatings
EN 23270, Paints and varnishes and their raw materials - Temperatures and humidities for conditioning
and testing (ISO 3270)
EN ISO 1513, Paints and varnishes - Examination and preparation of test samples (ISO 1513)
EN ISO 15528, Paints, varnishes and raw materials for paints and varnishes - Sampling (ISO 15528)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
active substance
substance or micro-organism that has an action on or against harmful organisms
[SOURCE: Biocidal Product Regulation (BPR, Regulation (EU) 528/2012), Article 3.1 (c), modified – the
article “a” between “or” and “micro-organism” was deleted]
3.2
sample
portion of coating material to be tested
3.3
test sample
strip of filter paper without biocidal effect covered with the coating material to be tested
Note 1 to entry: See also Figure A.1.
3.4
test specimen
punched-out portion of a test sample
Note 1 to entry: See also Figure A.1.
4 Principle
To determine the fungicidal efficacy of film preservatives in a coating, the coating material is applied to a
substrate conditioned according to EN 23270, placed onto an agar surface, inoculated with a standard
fungal spore suspension and incubated. Conclusions can be drawn about the fungicidal efficacy of the film
preservatives in a coating from the intensity of the fungal growth on the surface of the test specimen after
incubation. The method described here is a semiquantitative, comparative method between coatings,
with and without film preservatives.
5 Apparatus and materials
5.1 Cutting device for preparing the test specimens (coated filter paper with a diameter of 55 mm).
5.2 Autoclave.
5.3 Incubator capable of maintaining (24 ± 2) °C.
5.4 Pipette, in the range between 100 µl to 1 000 µl, with sterile tips or combi-tips of 12,5 ml.
5.5 Filter paper without fungicidal effect (e.g. cellulose with a pore size of 0,45 µm and a thickness of
650 µm).
5.6 If applicable, automatic welding apparatus to seal the bags.
5.7 Sterilized glass bottles (100 ml).
5.8 Laboratory balance, capable of weighing to an accuracy of 0,1 g.
5.9 Microscope.
5.10 Device to determine cell count (commercially available counting chamber, e.g. Thoma chamber).
5.11 Wetting agent (e.g. N-methyltaurine, sodium lauryl sulfate).
5.12 Device for applying the coating.
5.13 Sterilized test tubes or other sterilized glassware for preparing slant agar cultures.
5.14 Sterile Drigalski spatula or disposable spreader.
5.15 Sterile platinum loop or disposable loops.
5.16 Sterile glass funnel with cotton wool or rockwool.
5.17 Sterile Petri dishes (with a diameter of 94 mm, and a height of 16 mm).
5.18 Sterile tweezers.
5.19 Sterile water.
5.20 Malt extract agar.
5.21 Class 2 microbiological safety cabinet according to EN 12469.
6 Fungi
6.1 Fungi more likely to grow in an exterior environment
a) Aureobasidium pullulans (DSM 2404)
b) Alternaria alternata (DSM 62010)
c) Cladosporium cladosporioides (DSM 62121)
d) Phoma violaceae (IMI 49948ii)
e) Ulocladium atrum (IMI 79906 or DSMZ 63068)
6.2 Fungi more likely to grow in an interior environment
a) Aspergillus versicolor (DSM 1943)
b) Aspergillus niger (DSM 12634)
c) Stachybotrys chartarum (DSMZ 2144)
...
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