EN 113-3:2023
(Main)Durability of wood and wood-based products - Test method against wood destroying basidiomycetes - Part 3: Assessment of durability of wood-based panels
Durability of wood and wood-based products - Test method against wood destroying basidiomycetes - Part 3: Assessment of durability of wood-based panels
This document describes a method for assessing the durability of wood-based panels or analogue wood products to attack by wood-destroying basidiomycete fungi growing in pure culture.
The test method described in this document is intended to complement EN 113-2 with focus on specific aspects of wood-based panels or analogue wood products. This document is not intended to determine the effectiveness of wood preservatives used to prevent decay, which is covered by EN 113-1.
NOTE This method can be used in conjunction with an appropriate ageing procedure, for example EN 73 or EN 84.
The method is applicable to uncoated, rigid wood-based panel products. It is applicable to the determination of the decay resistance of wood-based panel products:
- made from naturally durable materials;
- made from materials treated with preservatives prior to manufacture;
- treated with a preservative which is introduced during manufacture, for example as an additive to the adhesive;
- specific treatments to increase durability of wood-based panels, e.g. wood modification.
Annex A (informative) contains a guidance on sampling.
Annex B (normative) contains some methods of sterilization.
Annex C (informative) contains information on the culture vessels.
Annex D (informative) contains an example of a test report.
Annex E (informative) contains information on the test fungi.
Annex F (informative) contains the assessment of the results.
Annex G (informative) contains extra info on moisture dynamics, coatings, composites and impact of dimensions.
Dauerhaftigkeit von Holz und Holzprodukten - Prüfverfahren in Bezug auf Holz zerstörende Basidiomyceten - Teil 3: Bewertung der Dauerhaftigkeit von Holzwerkstoffen
Dieses Dokument beschreibt ein Verfahren zur Bewertung der Dauerhaftigkeit von Holzwerkstoffen oder entsprechenden Holzprodukten gegen den Angriff von Holz zerstörenden, in Reinkultur wachsenden Basidiomyceten.
Das in diesem Dokument beschriebene Prüfverfahren dient dazu, EN 113 2 im Hinblick auf bestimmte Eigenschaften von Holzwerkstoffen oder entsprechenden Holzprodukten zu ergänzen. Dieses Dokument dient nicht dazu, die Wirksamkeit von Holzschutzmitteln, die zur Fäulnisverhinderung eingesetzt werden, zu bestimmen, die in EN 113 1 behandelt wird.
ANMERKUNG Dieses Verfahren kann in Verbindung mit einer geeigneten Alterungsbeanspruchung angewendet werden, z. B. EN 73 oder EN 84.
Das Verfahren ist für unbeschichtete, starre Holzwerkstoffe anwendbar. Es ist anwendbar zur Bestimmung der Widerstandsfähigkeit gegen Fäulnis bei Holzwerkstoffprodukten:
— die aus natürlich dauerhaften Materialien hergestellt wurden;
— die aus Materialien hergestellt wurden, die zuvor mit Holzschutzmitteln behandelt wurden;
— die mit einem Holzschutzmittel behandelt wurden, das während der Herstellung eingebracht wurde, z. B. als Zusatzstoff zu einem Klebstoff;
— mit spezifischen Behandlungen zur Erhöhung der Dauerhaftigkeit von Holzwerkstoffen, wie z. B. Modifi¬zierung von Holz.
Anhang A (informativ) enthält eine Anleitung zur Probenahme.
Anhang B (normativ) enthält einige Sterilisationsverfahren.
Anhang C (informativ) enthält Informationen zu den Prüfgefäßen.
Anhang D (informativ) enthält ein Beispiel für einen Prüfbericht.
Anhang E (informativ) enthält Informationen zu den Prüfpilzen.
Anhang F (informativ) enthält die Bewertung der Ergebnisse.
Anhang G (informativ) enthält zusätzliche Informationen zu Feuchtedynamik, Beschichtungen, Verbund¬werkstoffen und zum Einfluss der Maße.
Durabilité du bois et des matériaux dérivés du bois - Méthode d’essai contre les champignons basidiomycètes lignivores - Partie 3 : Évaluation de la durabilité des panneaux à base de bois
Trajnost lesa in lesnih proizvodov - Preskusna metoda proti glivam prostotrosnicam, ki uničujejo les - 3. del: Ocenjevanje odpornosti lesenih plošč
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN 113-3:2023
01-junij-2023
Nadomešča:
SIST-TS ENV 12038:2004
Trajnost lesa in lesnih proizvodov - Preskusna metoda proti glivam
prostotrosnicam, ki uničujejo les - 3. del: Ocenjevanje odpornosti lesenih plošč
Durability of wood and wood-based products - Test method against wood destroying
basidiomycetes - Part 3: Assessment of durability of wood-based panels
Dauerhaftigkeit von Holz und Holzprodukten - Prüfverfahren in Bezug auf Holz
zerstörende Basidiomyceten - Teil 3: Bewertung der Dauerhaftigkeit von Holzwerkstoffen
Durabilité du bois et des matériaux dérivés du bois - Méthode d’essai contre leschampignons basidiomycètes lignivores - Partie 3 : Évaluation de la durabilité des
panneaux à base de boisTa slovenski standard je istoveten z: EN 113-3:2023
ICS:
79.060.01 Lesne plošče na splošno Wood-based panels in
general
SIST EN 113-3:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN 113-3:2023
EN 113-3
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2023
EUROPÄISCHE NORM
ICS 79.060.01 Supersedes ENV 12038:2002
English Version
Durability of wood and wood-based products - Test
method against wood destroying basidiomycetes - Part 3:
Assessment of durability of wood-based panels
Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten -
Méthode d'essai contre les champignons Prüfverfahren in Bezug auf Holz zerstörende
basidiomycètes lignivores - Partie 3 : Évaluation de la Basidiomyceten - Teil 3: Bewertung der
durabilité des panneaux à base de bois Dauerhaftigkeit von HolzwerkstoffenThis European Standard was approved by CEN on 25 December 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 113-3:2023 E
worldwide for CEN national Members.---------------------- Page: 3 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
Contents Page
European foreword ....................................................................................................................................................... 4
Introduction .................................................................................................................................................................... 5
1 Scope .................................................................................................................................................................... 6
2 Normative references .................................................................................................................................... 6
3 Terms and definitions ................................................................................................................................... 7
4 Principle ............................................................................................................................................................. 7
5 Test material and apparatus ....................................................................................................................... 7
5.1 Biological material .......................................................................................................................................... 7
5.1.1 Test fungi ............................................................................................................................................................ 7
5.1.2 Solid wood stock .............................................................................................................................................. 8
5.2 Products and reagents ................................................................................................................................... 9
5.2.1 Culture medium ............................................................................................................................................... 9
5.2.2 Additive for white rot fungi ......................................................................................................................... 9
5.3 Apparatus ........................................................................................................................................................ 10
5.3.1 Conditioning supports ................................................................................................................................ 10
5.3.2 Conditioning room ....................................................................................................................................... 10
5.3.3 Culture chamber ........................................................................................................................................... 10
5.3.4 Culture vessels .............................................................................................................................................. 10
5.3.5 Ventilated drying oven ............................................................................................................................... 10
5.3.6 Desiccators ..................................................................................................................................................... 10
5.3.7 Test specimens supports ........................................................................................................................... 10
5.3.8 Safety equipment and protective clothing .......................................................................................... 10
5.3.9 Equipment for steam sterilization or access to a radiation source ........................................... 10
5.3.10 Ordinary laboratory equipment ............................................................................................................. 10
6 Test specimens .............................................................................................................................................. 11
6.1 General ............................................................................................................................................................. 11
6.2 Test specimen preparation....................................................................................................................... 11
7 Numbers of test specimens ....................................................................................................................... 11
7.1 Test product specimens ............................................................................................................................. 11
7.1.1 Test specimens .............................................................................................................................................. 11
7.1.2 Moisture content check specimens ........................................................................................................ 11
7.1.3 Wetting check specimens .......................................................................................................................... 11
7.2 Virulence control specimens ................................................................................................................... 11
8 Procedure........................................................................................................................................................ 12
8.1 Pre-conditioning ........................................................................................................................................... 12
8.2 Initial dry mass ............................................................................................................................................. 12
8.2.1 Test product specimens ............................................................................................................................. 12
8.2.2 Virulence control specimens ................................................................................................................... 13
8.3 Sterilization of test specimens ................................................................................................................ 13
8.4 Preparation of the culture vessels ......................................................................................................... 13
8.5 Inoculation ...................................................................................................................................................... 13
8.6 Exposure of test specimens ...................................................................................................................... 13
8.6.1 Preparation of additive for white rot fungi ........................................................................................ 13
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EN 113-3:2023 (E)
8.6.2 Test specimens .............................................................................................................................................. 14
8.6.3 Virulence control specimens .................................................................................................................... 14
8.6.4 Wetting check specimens .......................................................................................................................... 14
8.7 Culture conditions and duration of the test ........................................................................................ 14
8.8 Assessment of the test ................................................................................................................................. 14
8.8.1 Examination of the test specimens ......................................................................................................... 14
8.8.2 Final dry mass ................................................................................................................................................ 14
8.8.3 Validity of results .......................................................................................................................................... 15
9 Validity of the test ......................................................................................................................................... 15
10 Assessment of results .................................................................................................................................. 15
11 Test report ...................................................................................................................................................... 16
Annex A (informative) Guidance on sampling ................................................................................................ 18
Annex B (normative) Methods of sterilization ................................................................................................ 19
B.1 Ionizing radiation ......................................................................................................................................... 19
B.2 Steam ................................................................................................................................................................. 19
Annex C (informative) Culture vessels ............................................................................................................... 20
Annex D (informative) Example of a test report............................................................................................. 23
Annex E (informative) Test fungi ......................................................................................................................... 26
E.1 General information on maintaining and acquisition of test strains ........................................ 26
E.2 Maintenance and treatment of test fungi ............................................................................................. 26
E.3 Information regarding obligatory test fungi ...................................................................................... 27
E.3.1 Coniophora puteana (Schumacher ex Fries) Karsten [Synonym: Coniophora cerebella
(Persoon) Duby] ............................................................................................................................................ 27
E.3.2 Pleurotus ostreatus (Jacquin ex Fries) Kummer ................................................................................ 27
E.3.3 Gloeophyllum trabeum (Persoon ex Fries) Murrill [Synonyms: Lenzites trabea(Persoon ex Fries) Fries - Trametes trabea (Persoon ex Fries) Bresadola] ............................ 27
E.3.4 Trametes versicolor (Linnaeus ex Fries) Pile [Synonyms: Polyporus versicolor
Linnaeus ex Fries - Polystictus versicolor (Linnaeus) Saccardo - Coriolus versicolor
(Linnaeus) Quelet] ....................................................................................................................................... 27
E.3.5 Rhodonia placenta (Fr.) Niemelä, K.H. Larss. and Schigel [Synonym: Poria placenta
(Fries) Cooke sensu J. Eriksson - Poria monticola Murrill] ............................................................ 28
E.4 Information regarding optional test fungi .......................................................................................... 28
Annex F (informative) Assessment of results .................................................................................................. 31
Annex G (informative) Moisture dynamics, coatings, composites and impact of dimensions....... 33
G.1 General information on moisture dynamics ....................................................................................... 33
G.2 Coated wood-based panels ........................................................................................................................ 33
G.3 Wood polymer composites and natural fibre composites ............................................................. 33
G.4 Impact of dimensions .................................................................................................................................. 34
Bibliography ................................................................................................................................................................. 35
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EN 113-3:2023 (E)
European foreword
This document (EN 113-3:2023) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by AFNOR.This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2023, and conflicting national standards shall be
withdrawn at the latest by August 2023.Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes ENV 12038:2002.Test results obtained with earlier versions of ENV 12038 are still valid.
Compared to current EN 113, modifications are the following:
— A third part has been included.
— The three parts of the new EN 113 deal with similar testing but relate to a different scope.
EN 113-3:2023 includes the following significant technical changes with respect to ENV 12038:2002:
— Change of the title;— The methods for sterilization are updated;
— All annexes are informative, except Annex B on sterilization methods;
— Some additional validity requirements are introduced for control specimens;
— Number of test specimens per fungus doubled;
— Number of virulence control specimens increased from 6 to 10;
— Size control specimens deleted;
— Assessment of results changed.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.---------------------- Page: 6 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
Introduction
This document describes a laboratory test method in which small samples of the wood-based panel
product under test are exposed to attack by a range of wood-destroying basidiomycete fungi in pure
culture. The thickness of the test specimens varies, since it is dictated by the thickness of the wood-based
panel product under test. The effect of constituents giving temporary protection is avoided by testing
after pre-conditioning of the cut specimens in a freely ventilated environment. The test method also
includes a minimum moisture uptake requirement.The procedures described in this document method are intended to be carried out by suitably trained
and/or supervised specialists. Appropriate safety precautions should be observed throughout the use of
this document.---------------------- Page: 7 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
1 Scope
This document describes a method for assessing the durability of wood-based panels or analogue wood
products to attack by wood-destroying basidiomycete fungi growing in pure culture.
The test method described in this document is intended to complement EN 113-2 with focus on specific
aspects of wood-based panels or analogue wood products. This document is not intended to determine
the effectiveness of wood preservatives used to prevent decay, which is covered by EN 113-1.
NOTE This method can be used in conjunction with an appropriate ageing procedure, for example EN 73 or
EN 84.The method is applicable to uncoated, rigid wood-based panel products. It is applicable to the
determination of the decay resistance of wood-based panel products:— made from naturally durable materials;
— made from materials treated with preservatives prior to manufacture;
— treated with a preservative which is introduced during manufacture, for example as an additive to
the adhesive;— specific treatments to increase durability of wood-based panels, e.g. wood modification.
Annex A (informative) contains a guidance on sampling.Annex B (normative) contains some methods of sterilization.
Annex C (informative) contains information on the culture vessels.
Annex D (informative) contains an example of a test report.
Annex E (informative) contains information on the test fungi.
Annex F (informative) contains the assessment of the results.
Annex G (informative) contains extra info on moisture dynamics, coatings, composites and impact of
dimensions.2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 84, Durability of wood and wood-based products - Accelerated ageing of treated wood prior to biological
testing - Leaching procedure---------------------- Page: 8 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https://www.electropedia.org/— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
supplier
sponsor of the test, person or company providing the sample of wood/timber (wood-based panel) to be
tested4 Principle
Specimens prepared from the wood-based panel product(s) under test, after pre-conditioning, and
control specimens of defined function are exposed to attack by pure cultures of wood-destroying
basidiomycete fungi.After a prescribed period of incubation under defined conditions, the loss in dry mass of the specimens
is used as the criterion for determining the extent of attack.5 Test material and apparatus
5.1 Biological material
5.1.1 Test fungi
5.1.1.1 General
The test fungi to be used are as follows and relate to corresponding virulence control specimens.
It is required that the reference timber virulence control specimens provide a median mass loss of at least
30 % with one of the test fungi.5.1.1.2 Obligatory test fungi for all types of panel products
— Coniophora puteana (Schumacher ex Fries) Karsten (BAM Ebw. 15)
Loss in mass of Scots pine sapwood virulence control specimens in 16 weeks: minimum 20 % (m/m).
— Pleurotus ostreatus (Jacquin ex Fries) Kummer (FPRL 40C)Loss in mass of beech virulence control specimens in 16 weeks: 20 % (m/m).
5.1.1.3 Species to be used compulsorily on the nature of the test product
For test products made only from softwood:
— Gloeophyllum trabeum (Persoon ex Fries) Murrill (BAM Ebw. 109)
Loss in mass of Scots pine sapwood virulence control specimens in 16 weeks: minimum 20 % (m/m).
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EN 113-3:2023 (E)
For test products made only from hardwood:
— Trametes versicolor (L.) Lloyd (CTB 863 A)
Loss in mass of beech virulence control specimens in 16 weeks: minimum 20 % (m/m).
For test products made from a mixture of softwood and hardwood, both Gloeophyllum trabeum and
Trametes versicolor shall be used.5.1.1.4 Additional obligatory test fungus for modified wood
— Rhodonia placenta (Fr.) Niemelä, K.H. Larss. and Schigel (FPRL 280).
Loss in mass of Scots pine sapwood in 16 weeks: minimum 20 % (m/m).
5.1.1.5 Optional test fungi
For specific regional uses or conditions, it is also possible to choose other fungi on an optional basis .
5.1.1.6 Maintenance of fungal strainsThe strains shall be maintained and treated (frequency of subculturing, alternation of culture media etc.)
in accordance with the instructions from their laboratory of origin (see Annex E). The parent strain shall
be maintained in the laboratory of its origin, so as to conserve and ensure its vigour.
If tests are not undertaken regularly, or if a strain shows signs of degeneration, a new standard culture of
the strain shall be obtained from the laboratory of origin for each test. When new strains are received,
the virulence shall be tested to ensure that the mass loss achieved is above the minimum value given in
Annex E.5.1.2 Solid wood stock
5.1.2.1 Wood species
The following species shall be used for the test:
— Scots pine sapwood (Pinus sylvestris L.)
— European beech (Fagus sylvatica L.)
5.1.2.2 Quality of the wood
The wood shall be free from visible cracks, stain, decay, insect damage or other defects. The wood shall
not have been water-stored, floated, chemically treated or steamed.NOTE Wood that has been kiln dried at temperatures not above 60 °C can be used.
The Scots pine shall be exclusively sapwood containing little resin and having between 2,5 and 8 annual
growth rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the
whole.The beech shall be even grained, free from tyloses and discoloration. It shall have between 2 and 6 annual
growth rings per 10 mm.1 Former name: Coriolus versicolor (Linnaeus) Quelet
See Annex E for a non-comprehensive list of recommended optional fungi.
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SIST EN 113-3:2023
EN 113-3:2023 (E)
5.1.2.3 Virulence control specimens
Prepare planed strips from the solid wood stock having a cross-section (25 ± 0,5) mm x (15 ± 0,5) mm.
The longitudinal faces shall be parallel to the direction of the grain. The annual rings shall not be parallel
to the faces (contact angle greater than 10°) but otherwise can run in any direction. Make transverse cuts,
neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give virulence control
specimens (50 ± 0,5) mm long.The dimensions of each virulence control specimen at a moisture content of (12 ± 2) % shall be
(50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.The specimens shall originate from a minimum of three trees or shall be taken from a stock originally of
more than 500 specimens.5.2 Products and reagents
5.2.1 Culture medium
The culture medium shall be a malt agar medium with the following composition:
— malt extract: in concentrated form: (50 ± 0,5) g; in powder form: (40 ± 0,5) g;
— agar causing no inhibition of growth of fungi: (20 ± 0,5) g to (30 ± 0,5) g;— de-ionized water; quantity to make up to 1 000 g.
— preferably use water conforming to grade 3 of EN ISO 3696.
Place in each culture vessel (5.3.4) a sufficient quantity of the medium to provide a minimum depth of
3 mm to 4 mm when in its in-use position. Close the vessels as specified in 5.3.4 and sterilize in an
autoclave at 121 °C for 20 min. Let the vessels cool in their in-use position.NOTE The quantity of culture medium required in each culture vessel varies with the thickness of the test
product (see 8.4).5.2.2 Additive for white rot fungi
Anhydrated, laminar, aluminium-iron-magnesium silicate exfoliated to yield particles up to 3 mm
diameter. Particles less than 2 mm diameter shall be removed by sieving. Before use, mix the sample of
additive well. The additive shall be used only once.This ‘vermiculite’ additive is always required in combination with the test fungus Pleurotus ostreatus.
This ‘vermiculite’ additive is also required for Trametes versicolor when thicker panels (> 20 mm) are
under test.A moisture meter of the two pronged electrical conductivity type is suitable for assessing moisture content, but
oven drying is used commonly to assess moisture content.Vermiculite is suitable.
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SIST EN 113-3:2023
EN 113-3:2023 (E)
5.3 Apparatus
5.3.1 Conditioning supports
Made of glass, stainless steel or any other inert material, that is to say with no risk of having any effect on
the test specimens. The supports shall provide free circulation of air around the test specimens whilst
having a minimum of contact with the test specimens.5.3.2 Conditioning room
Well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative humidity.
5.3.3 Culture chamber
Incubator or room, dark and controlled at (22 ± 2) °C and (70 ± 5) % relative humidity.
5.3.4 Culture vesselsKolle flasks or equivalent vessels with a capacity of between 400 ml and 650 ml providing a flat surface
2 2area of between 85 cm and 120 cm for the medium and close with a material that allows for air
exchange.NOTE 1 Examples of suitable vessels are given in Annex C.
NOTE 2 Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with
leakproof lids, the centres of which are pierced with a round hole of up to 15 mm diameter and plugged with a wad
of cotton wool.5.3.5 Ventilated drying oven
Capable of being controlled at (103 ± 2) °C.
5.3.6 Desiccators
With an efficient desiccant, for example silica gel.
5.3.7 Test specimens supports
Made of glass, stainless steel or any other inert material, that is to say with no risk of having any effect on
the culture medium, the fungus, the wood, or of modifying itself. The supports are used to prevent direct
contact of the specimens with the culture medium, but shall not separate them from it by more than 3 mm.
If abnormally high moisture contents are experienced consistently, use of specimen supports of
approximately 5 mm thick may help to control the problem. If thicker specimen supports are used, this
should be recorded in the test report. Abnormally high moistur...
SLOVENSKI STANDARD
oSIST prEN 113-3:2021
01-november-2021
Trajnost lesa in lesnih proizvodov - Preskusna metoda proti glivam
prostotrosnicam, ki uničujejo les - 3. del: Ocenjevanje trajnosti lesenih plošč
Durability of wood and wood-based products - Test method against wood destroying
basidiomycetes - Part 3: Assessment of durability of wood-based panels
Dauerhaftigkeit von Holz und Holzprodukten - Prüfverfahren in Bezug auf Holz
zerstörende Basidiomyceten - Teil 3: Bewertung der Dauerhaftigkeit von Holzwerkstoffen
Durabilité du bois et des matériaux dérivés du bois - Méthode d’essai contre leschampignons basidiomycètes lignivores - Partie 3 : Évaluation de la durabilité des
panneaux à base de boisTa slovenski standard je istoveten z: prEN 113-3
ICS:
79.060.01 Lesne plošče na splošno Wood-based panels in
general
oSIST prEN 113-3:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------oSIST prEN 113-3:2021
---------------------- Page: 2 ----------------------
oSIST prEN 113-3:2021
DRAFT
EUROPEAN STANDARD
prEN 113-3
NORME EUROPÉENNE
EUROPÄISCHE NORM
September 2021
ICS 79.060.01 Will supersede ENV 12038:2002
English Version
Durability of wood and wood-based products Test method
against wood destroying basidiomycetes - Part 3:
assessment of durability of wood-based panels
Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten -
Méthode d'essai contre les champignons Prüfverfahren in Bezug auf Holz zerstörende
basidiomycètes lignivores - Partie 3 : Évaluation de la Basidiomyceten - Teil 3: Bewertung der
durabilité des panneaux à base de bois Dauerhaftigkeit von HolzwerkstoffenThis draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 113-3:2021 E
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Contents Page
European foreword ....................................................................................................................................................... 4
Introduction .................................................................................................................................................................... 5
1 Scope .................................................................................................................................................................... 6
2 Normative references .................................................................................................................................... 6
3 Terms and definitions ................................................................................................................................... 7
4 Principle ............................................................................................................................................................. 7
5 Test material and apparatus ....................................................................................................................... 7
5.1 Biological material .......................................................................................................................................... 7
5.1.1 Test fungi ............................................................................................................................................................ 7
5.1.2 Solid wood stock .............................................................................................................................................. 8
5.2 Products and reagents ................................................................................................................................... 9
5.2.1 Culture medium ............................................................................................................................................... 9
5.2.2 Additive for white rot fungi ......................................................................................................................... 9
5.3 Apparatus ........................................................................................................................................................ 10
5.3.1 Conditioning supports ................................................................................................................................ 10
5.3.2 Conditioning room ....................................................................................................................................... 10
5.3.3 Culture chamber ........................................................................................................................................... 10
5.3.4 Culture vessels .............................................................................................................................................. 10
5.3.5 Ventilated drying oven ............................................................................................................................... 10
5.3.6 Desiccators ..................................................................................................................................................... 10
5.3.7 Test specimens supports ........................................................................................................................... 10
5.3.8 Safety equipment and protective clothing .......................................................................................... 10
5.3.9 Equipment for steam sterilization or access to a radiation source ........................................... 10
5.3.10 Ordinary laboratory equipment ............................................................................................................. 10
6 Test specimens .............................................................................................................................................. 11
6.1 General ............................................................................................................................................................. 11
6.2 Test specimen preparation....................................................................................................................... 11
7 Numbers of test specimens ....................................................................................................................... 11
7.1 Test product specimens ............................................................................................................................. 11
7.1.1 Test specimens .............................................................................................................................................. 11
7.1.2 Moisture content check specimens ........................................................................................................ 11
7.1.3 Wetting check specimens .......................................................................................................................... 11
7.2 Virulence control specimens ................................................................................................................... 12
8 Procedure........................................................................................................................................................ 12
8.1 Pre-conditioning ........................................................................................................................................... 12
8.2 Initial dry mass ............................................................................................................................................. 13
8.2.1 Test product specimens ............................................................................................................................. 13
8.2.2 Virulence control specimens ................................................................................................................... 13
8.3 Sterilization of test specimens ................................................................................................................ 13
8.4 Preparation of the culture vessels ......................................................................................................... 13
8.5 Inoculation ...................................................................................................................................................... 14
8.6 Exposure of test specimens ...................................................................................................................... 14
8.6.1 Preparation of additive for white rot fungi ........................................................................................ 14
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8.6.2 Test specimens .............................................................................................................................................. 14
8.6.3 Virulence control specimens .................................................................................................................... 14
8.6.4 Wetting check specimens .......................................................................................................................... 14
8.7 Culture conditions and duration of the test ........................................................................................ 14
8.8 Assessment of the test ................................................................................................................................. 15
8.8.1 Examination of the test specimens ......................................................................................................... 15
8.8.2 Final dry mass ................................................................................................................................................ 15
8.8.3 Validity of results .......................................................................................................................................... 15
9 Validity of the test ......................................................................................................................................... 15
10 Assessment of results .................................................................................................................................. 16
11 Test report ...................................................................................................................................................... 16
Annex A (informative) Guidance on sampling ................................................................................................ 18
Annex B (normative) Methods of sterilization ................................................................................................ 19
B.1 Ionizing radiation ......................................................................................................................................... 19
B.2 Steam ................................................................................................................................................................. 19
Annex C (informative) Culture vessels ............................................................................................................... 20
Annex D (informative) Example of a test report............................................................................................. 23
Annex E (informative) Test fungi ......................................................................................................................... 26
E.1 General information on maintaining and acquisition of test strains ........................................ 26
E.2 Maintenance and treatment of test fungi ............................................................................................. 26
E.3 Information regarding obligatory test fungi ...................................................................................... 27
E.3.1 Coniophora puteana (Schumacher ex Fries) Karsten [Synonym: Coniophora cerebella
(Persoon) Duby] ............................................................................................................................................ 27
E.3.2 Pleurotus ostreatus (Jacquin ex Fries) Kummer ................................................................................ 27
E.3.3 Gloeophyllum trabeum (Persoon ex Fries) Murrill [Synonyms: Lenzites trabea(Persoon ex Fries) Fries - Trametes trabea (Persoon ex Fries) Bresadola] ............................ 27
E.3.4 Trametes versicolor (Linnaeus ex Fries) Pile [Synonyms: Polyporus versicolor
Linnaeus ex Fries - Polystictus versicolor (Linnaeus) Saccardo - Coriolus versicolor
(Linnaeus) Quelet ]....................................................................................................................................... 27
E.3.5 Rhodonia placenta (Fr.) Niemelä, K.H. Larss. and Schigel [Synonym: Poria placenta
(Fries) Cooke sensu J. Eriksson - Poria monticola Murrill] ............................................................ 28
E.4 Information regarding optional test fungi .......................................................................................... 28
Annex F (informative) Assessment of results .................................................................................................. 31
Annex G (informative) Moisture dynamics, coatings, composites and impact of dimensions....... 33
G.1 General information on moisture dynamics ....................................................................................... 33
G.2 Coated wood-based panels ........................................................................................................................ 33
G.3 Wood polymer composites and Natural fibre composites............................................................. 33
G.4 Impact of dimensions .................................................................................................................................. 33
Bibliography ................................................................................................................................................................. 35
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European foreword
This document (prEN 113-3:2021) has been prepared by Technical Committee CEN/TC 38 “Durability of
Wood and wood-based products”, the secretariat of which is held by AFNOR.This document is currently submitted to the CEN Enquiry.
This document will supersede ENV 12038:2002.
Test results obtained with earlier versions of ENV 12038 are still valid.
Compared to current EN 113, the following modifications are:
— A third part has been included.
— The three parts of the new EN 113 deal with similar testing but relate to a different scope.
Compared to the ENV 12038:2002 version the following major changes have been introduced:
— Change of the title;— The methods for sterilization are updated;
— All annexes are informative;
— Some additional validity requirements are introduced for control specimens.
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Introduction
This document describes a laboratory test method in which small samples of the wood-based panel
product under test are exposed to attack by a range of wood-destroying basidiomycete fungi in pure
culture. The thickness of the test specimens varies, since it is dictated by the thickness of the wood-based
panel product under test. The effect of constituents giving temporary protection is avoided by testing
after pre-conditioning of the cut specimens in a freely ventilated environment. The test method also
includes a minimum moisture uptake requirement.The procedures described in this document method are intended to be carried out by suitably trained
and/or supervised specialists. Appropriate safety precautions should be observed throughout the use of
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1 Scope
This document describes a method for assessing the durability of wood-based panels or analogous wood
products to attack by wood-destroying basidiomycete fungi growing in pure culture.
The test method described in this document is intended to complement EN 113-2 with focus on specific
aspects of wood-based panels or analogue wood products. This document is not intended to determine
the effectiveness of wood preservatives used to prevent decay which is covered by EN 113-1.
NOTE 1 Determining the efficacy of wood preservatives used to prevent decay is the scope of EN 113-1.
NOTE 2 This method may be used in conjunction with an ageing procedure, for example EN 73 or EN 84.
NOTE 3 Complementary to EN 113-2 this part focusses on specific test procedure for wood-based panels or
analogous wood products.The method is applicable to uncoated, rigid wood-based panel products. It is applicable to the
determination of the decay resistance of wood-based panel products:— made from naturally durable materials;
— made from materials treated with preservatives prior to manufacture;
— treated with a preservative which is introduced during manufacture, for example as an additive to
the adhesive;— specific treatments to increase durability of wood-based panels, e.g. wood modification
NOTE 4 This method can be used in conjunction with an appropriate ageing procedure, for example EN 73 or
EN 84.Annex A (informative) contains a guidance on sampling.
Annex B (normative) contains some methods of sterilization.
Annex C (informative) contains information on the culture vessels.
Annex D (informative) contains an example of a test report.
Annex E (informative) contains information on the test fungi.
Annex F (informative) contains the assessment of the results.
Annex G (informative) contains extra info on moisture dynamics, coatings, composites and impact of
dimensions2 Normative references
There are no normative references in this document.
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3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at http://www.electropedia.org/— ISO Online browsing platform: available at http://www.iso.org/obp
3.1
supplier
sponsor of the test, person or company providing the sample of wood/timber (wood-based panel) to be
tested4 Principle
Specimens prepared from the wood-based panel product(s) under test, after pre-conditioning, and
control specimens of defined function are exposed to attack by pure cultures of wood-destroying
basidiomycete fungi.After a prescribed period of incubation under defined conditions, the loss in dry mass of the specimens
is used as the criterion for determining the extent of attack.5 Test material and apparatus
5.1 Biological material
5.1.1 Test fungi
The test fungi to be used are as follows and relate to corresponding virulence control specimens.
It is required that the reference timber virulence control specimens provide a median mass loss of at least
30 % with one of the test fungi.5.1.1.1 Obligatory test fungi for all types of panel products
— Coniophora puteana (Schumacher ex Fries) Karsten (BAM Ebw. 15)
Loss in mass of Scots pine sapwood virulence control specimens in 16 weeks: a mass fraction of
minimum 20 %— Pleurotus ostreatus (Jacquin ex Fries) Quélet (FPRL 40C)
Loss in mass of beech virulence control specimens in 16 weeks: a mass fraction of minimum 20 %
5.1.1.2 Species to be used compulsorily on the nature of the test productFor test products made only from softwood:
— Gloeophyllum trabeum (Persoon ex Fries) Murrill (BAM Ebw. 109)
Loss in mass of Scots pine sapwood virulence control specimens in16 weeks: a mass fraction of minimum
20 %For test products made only from hardwood:
— Trametes versicolor (Linnaeus) Quélet (CTB 863A)
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Loss in mass of beech virulence control specimens in 16 weeks: a mass fraction of minimum 20 %
For test products made from a mixture of softwood and hardwood, both Gloeophyllum trabeum and
Trametes versicolor shall be used.5.1.1.3 Additional obligatory test fungus for modified wood
— Rhodonia placenta (Fr.) Niemelä, K.H. Larss. and Schigel (FPRL 280) for softwoods.
Loss in mass of Scots pine sapwood in 16 weeks: a mass fraction of minimum 20 %.5.1.1.4 Optional test fungi
For specific regional uses or conditions, it is also possible to choose other fungi on an optional basis .
5.1.1.5 Maintenance of fungal strainsThe strains shall be maintained and treated (frequency of subculturing, alternation of culture media etc.)
in accordance with the instructions from their laboratory of origin (see Annex E). The parent strain shall
be maintained in the laboratory of its origin, so as to conserve and ensure its vigour.
If tests are not undertaken regularly, or if a strain shows signs of degeneration, a new standard culture of
the strain shall be obtained from the laboratory of origin for each test. When new strains are received,
the virulence shall be tested to ensure that the mass loss achieved is above the minimum value given in
Annex E.5.1.2 Solid wood stock
5.1.2.1 Wood species
The following species shall be used for the test:
— Scots pine sapwood (Pinus sylvestris Linnaeus)
— European beech (Fagus sylvatica Linnaeus)
5.1.2.2 Quality of the wood
The wood shall be free from visible cracks, stain, decay, insect damage or other defects. The wood shall
not have been water-stored, floated, chemically treated or steamed.NOTE Wood that has been kiln dried at temperatures below 60 °C may be used.
The Scots pine shall be exclusively sapwood containing little resin and having between 2,5 and 8 annual
growth rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the
whole.The beech shall be even grained, free from tyloses and discoloration. It shall have between 2 and 6 annual
growth rings per 10 mm.See Annex E for a non-comprehensive list of recommended optional fungi.
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5.1.2.3 Virulence control specimens
Prepare planed strips from the solid wood stock having a cross-section (25 ± 0,5) mm x (15 ± 0,5) mm.
The longitudinal faces shall be parallel to the direction of the grain. The annual rings shall not be parallel
to the faces (contact angle greater than 10°) but otherwise can run in any direction. Make transverse cuts,
neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give virulence control
specimens (50 ± 0,5) mm long.The dimensions of each virulence control specimen at a mass fraction of (12 ± 2) % moisture content
shall be (50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.The specimens shall originate from a minimum of three trees or shall be taken from a stock originally of
more than 500 specimens.5.2 Products and reagents
5.2.1 Culture medium
The culture medium shall be a malt agar medium with the following composition:
— malt extract: in concentrated form: (50 ± 0,5) g; in powder form: (40 ± 0,5) g;
— agar causing no inhibition of growth of fungi: (20 ± 0,5) g to (30 ± 0,5) g;— de-ionized water; quantity to make up to 1000 g.
NOTE 1 Preferably use water conforming to grade 3 of ISO 3696.
Place in each culture vessel (5.3.1) a sufficient quantity of the medium to provide a minimum depth of
3 mm to 4 mm when in its in-use position. Close the vessels as specified in 5.3.1 and sterilize in an
autoclave at 121 °C for 20 min. Let the vessels cool in their in-use position.NOTE 2 The quantity of culture medium required in each culture vessel varies with the thickness of the test
product (see 8.4).5.2.2 Additive for white rot fungi
Anhydrated, laminar, aluminium-iron-magnesium silicate exfoliated to yield particles up to 3 mm
diameter. Particles less than 2 mm diameter shall be removed by sieving. Before use, mix the sample of
additive well. The additive shall be used only once.This ‘vermiculite’ additive is always required in combination with the test fungus Pleurotus ostreatus.
This ‘vermiculite’ additive is also required for Trametes versicolor when thicker panels (>20 mm) are
under test.Maximum thickness of panels that can be tested is 20 mm for brown rot fungi and 40 mm for white rot
fungi (see Annex G).A moisture meter of the two pronged electrical conductivity type is suitable for assessing moisture content.
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5.3 Apparatus
5.3.1 Conditioning supports
Made of glass, stainless steel or any other inert material, that is to say with no risk of having any effect on
the test specimens. The supports shall provide free circulation of air around the test specimens whilst
having a minimum of contact with the test specimens.5.3.2 Conditioning room
Well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative humidity.
5.3.3 Culture chamber
Incubator or room, dark and controlled at (22 ± 2) °C and (70 ± 5) % relative humidity.
5.3.4 Culture vesselsKolle flasks or equivalent vessels with a capacity of between 400 ml and 650 ml providing a flat surface
2 2area of between 85 cm and 120 cm for the medium and close with a material that allows for air
exchange.NOTE 1 Examples of suitable vessels are given in Annex C.
NOTE 2 Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with
leakproof lids, the centres of which are pierced with a round hole of up to 15 mm diameter and plugged with a wad
of cotton wool.5.3.5 Ventilated drying oven
Capable of being controlled at (103 ± 2) °C.
5.3.6 Desiccators
With an efficient desiccant, for example silica gel.
5.3.7 Test specimens supports
Made of glass, stainless steel or any other inert material, that is to say with no risk of having any effect on
the culture medium, the fungus, the wood, or of modifying itself. The supports are used to prevent direct
contact of the specimens with the culture medium, but shall not separate them from it by more than 3 mm.
If abnormally high moisture contents are experienced consistently, use of specimen supports of
approximately 5 mm thick may help to control the problem. If thicker specimen supports are used, this
should be recorded in the test report. Abnormally high moisture contents are those values of final
moisture content that are greater than 100 % (m/m).5.3.8 Safety equipment and protective clothing
Appropriate for the test procedures, to ensure the safety of the operator.
5.3.9 Equipment for steam sterilization or access to a radiation source
See Annex B.
5.3.10 Ordinary laboratory equipment
Including a balance capable of weighing to an accuracy of 0,01 g.
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6 Test specimens
6.1 General
A minimum of three replicate panels of the wood-based panel product under test shall be sampled.
Ensure that the panels are clean and as free as possible of contaminants that might give misleading
results. More details on sampling can be found in Annex A.6.2 Test specimen preparation
Reject from each of the three panels of the test product a strip 300 mm
...
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