Durability of wood and wood-based products - Test method against wood destroying basidiomycetes - Part 3: Assessment of durability of wood-based panels

This document describes a method for assessing the durability of wood-based panels or analogue wood products to attack by wood-destroying basidiomycete fungi growing in pure culture.
The test method described in this document is intended to complement EN 113-2 with focus on specific aspects of wood-based panels or analogue wood products. This document is not intended to determine the effectiveness of wood preservatives used to prevent decay, which is covered by EN 113-1.
NOTE   This method can be used in conjunction with an appropriate ageing procedure, for example EN 73 or EN 84.
The method is applicable to uncoated, rigid wood-based panel products. It is applicable to the determination of the decay resistance of wood-based panel products:
-   made from naturally durable materials;
-   made from materials treated with preservatives prior to manufacture;
-   treated with a preservative which is introduced during manufacture, for example as an additive to the adhesive;
-   specific treatments to increase durability of wood-based panels, e.g. wood modification.
Annex A (informative) contains a guidance on sampling.
Annex B (normative) contains some methods of sterilization.
Annex C (informative) contains information on the culture vessels.
Annex D (informative) contains an example of a test report.
Annex E (informative) contains information on the test fungi.
Annex F (informative) contains the assessment of the results.
Annex G (informative) contains extra info on moisture dynamics, coatings, composites and impact of dimensions.

Dauerhaftigkeit von Holz und Holzprodukten - Prüfverfahren in Bezug auf Holz zerstörende Basidiomyceten - Teil 3: Bewertung der Dauerhaftigkeit von Holzwerkstoffen

Dieses Dokument beschreibt ein Verfahren zur Bewertung der Dauerhaftigkeit von Holzwerkstoffen oder entsprechenden Holzprodukten gegen den Angriff von Holz zerstörenden, in Reinkultur wachsenden Basidiomyceten.
Das in diesem Dokument beschriebene Prüfverfahren dient dazu, EN 113 2 im Hinblick auf bestimmte Eigenschaften von Holzwerkstoffen oder entsprechenden Holzprodukten zu ergänzen. Dieses Dokument dient nicht dazu, die Wirksamkeit von Holzschutzmitteln, die zur Fäulnisverhinderung eingesetzt werden, zu bestimmen, die in EN 113 1 behandelt wird.
ANMERKUNG   Dieses Verfahren kann in Verbindung mit einer geeigneten Alterungsbeanspruchung angewendet werden, z. B. EN 73 oder EN 84.
Das Verfahren ist für unbeschichtete, starre Holzwerkstoffe anwendbar. Es ist anwendbar zur Bestimmung der Widerstandsfähigkeit gegen Fäulnis bei Holzwerkstoffprodukten:
—   die aus natürlich dauerhaften Materialien hergestellt wurden;
—   die aus Materialien hergestellt wurden, die zuvor mit Holzschutzmitteln behandelt wurden;
—   die mit einem Holzschutzmittel behandelt wurden, das während der Herstellung eingebracht wurde, z. B. als Zusatzstoff zu einem Klebstoff;
—   mit spezifischen Behandlungen zur Erhöhung der Dauerhaftigkeit von Holzwerkstoffen, wie z. B. Modifi¬zierung von Holz.
Anhang A (informativ) enthält eine Anleitung zur Probenahme.
Anhang B (normativ) enthält einige Sterilisationsverfahren.
Anhang C (informativ) enthält Informationen zu den Prüfgefäßen.
Anhang D (informativ) enthält ein Beispiel für einen Prüfbericht.
Anhang E (informativ) enthält Informationen zu den Prüfpilzen.
Anhang F (informativ) enthält die Bewertung der Ergebnisse.
Anhang G (informativ) enthält zusätzliche Informationen zu Feuchtedynamik, Beschichtungen, Verbund¬werkstoffen und zum Einfluss der Maße.

Durabilité du bois et des matériaux dérivés du bois - Méthode d’essai contre les champignons basidiomycètes lignivores - Partie 3 : Évaluation de la durabilité des panneaux à base de bois

Trajnost lesa in lesnih proizvodov - Preskusna metoda proti glivam prostotrosnicam, ki uničujejo les - 3. del: Ocenjevanje odpornosti lesenih plošč

General Information

Status

Published

Publication Date

07-Feb-2023

ICS

79.060.01 - Wood-based panels in general

Technical Committee

CEN/TC 38 - Durability of wood and derived materials

Drafting Committee

CEN/TC 38/WG 23 - Fungal testing (basidiomycetes-microfungi)

Current Stage

6060 - Definitive text made available (DAV) - Publishing

Start Date

08-Feb-2023

Due Date

21-Dec-2022

Completion Date

08-Feb-2023

Ref Project

SIST EN 113-3:2023 - Durability of wood and wood-based products - Test method against wood destroying basidiomycetes - Part 3: Assessment of durability of wood-based panels

Relations

Replaces

ENV 12038:2002 - Durability of wood and wood-based products - Wood-based panels - Method of test for determining the resistance against wood-destroying basidiomycetes

Effective Date

22-Feb-2017

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SLOVENSKI STANDARD
SIST EN 113-3:2023
01-junij-2023
Nadomešča:
SIST-TS ENV 12038:2004
Trajnost lesa in lesnih proizvodov - Preskusna metoda proti glivam
prostotrosnicam, ki uničujejo les - 3. del: Ocenjevanje odpornosti lesenih plošč
Durability of wood and wood-based products - Test method against wood destroying
basidiomycetes - Part 3: Assessment of durability of wood-based panels
Dauerhaftigkeit von Holz und Holzprodukten - Prüfverfahren in Bezug auf Holz

zerstörende Basidiomyceten - Teil 3: Bewertung der Dauerhaftigkeit von Holzwerkstoffen

Durabilité du bois et des matériaux dérivés du bois - Méthode d’essai contre les

champignons basidiomycètes lignivores - Partie 3 : Évaluation de la durabilité des

panneaux à base de bois
Ta slovenski standard je istoveten z: EN 113-3:2023
ICS:
79.060.01 Lesne plošče na splošno Wood-based panels in
general
SIST EN 113-3:2023 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
SIST EN 113-3:2023
---------------------- Page: 2 ----------------------
SIST EN 113-3:2023
EN 113-3
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2023
EUROPÄISCHE NORM
ICS 79.060.01 Supersedes ENV 12038:2002
English Version
Durability of wood and wood-based products - Test
method against wood destroying basidiomycetes - Part 3:
Assessment of durability of wood-based panels

Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten -

Méthode d'essai contre les champignons Prüfverfahren in Bezug auf Holz zerstörende

basidiomycètes lignivores - Partie 3 : Évaluation de la Basidiomyceten - Teil 3: Bewertung der

durabilité des panneaux à base de bois Dauerhaftigkeit von Holzwerkstoffen
This European Standard was approved by CEN on 25 December 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this

European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references

concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN

member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by

translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management

Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and

United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

worldwide for CEN national Members.
---------------------- Page: 3 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
Contents Page

European foreword ....................................................................................................................................................... 4

Introduction .................................................................................................................................................................... 5

1 Scope .................................................................................................................................................................... 6

2 Normative references .................................................................................................................................... 6

3 Terms and definitions ................................................................................................................................... 7

4 Principle ............................................................................................................................................................. 7

5 Test material and apparatus ....................................................................................................................... 7

5.1 Biological material .......................................................................................................................................... 7

5.1.1 Test fungi ............................................................................................................................................................ 7

5.1.2 Solid wood stock .............................................................................................................................................. 8

5.2 Products and reagents ................................................................................................................................... 9

5.2.1 Culture medium ............................................................................................................................................... 9

5.2.2 Additive for white rot fungi ......................................................................................................................... 9

5.3 Apparatus ........................................................................................................................................................ 10

5.3.1 Conditioning supports ................................................................................................................................ 10

5.3.2 Conditioning room ....................................................................................................................................... 10

5.3.3 Culture chamber ........................................................................................................................................... 10

5.3.4 Culture vessels .............................................................................................................................................. 10

5.3.5 Ventilated drying oven ............................................................................................................................... 10

5.3.6 Desiccators ..................................................................................................................................................... 10

5.3.7 Test specimens supports ........................................................................................................................... 10

5.3.8 Safety equipment and protective clothing .......................................................................................... 10

5.3.9 Equipment for steam sterilization or access to a radiation source ........................................... 10

5.3.10 Ordinary laboratory equipment ............................................................................................................. 10

6 Test specimens .............................................................................................................................................. 11

6.1 General ............................................................................................................................................................. 11

6.2 Test specimen preparation....................................................................................................................... 11

7 Numbers of test specimens ....................................................................................................................... 11

7.1 Test product specimens ............................................................................................................................. 11

7.1.1 Test specimens .............................................................................................................................................. 11

7.1.2 Moisture content check specimens ........................................................................................................ 11

7.1.3 Wetting check specimens .......................................................................................................................... 11

7.2 Virulence control specimens ................................................................................................................... 11

8 Procedure........................................................................................................................................................ 12

8.1 Pre-conditioning ........................................................................................................................................... 12

8.2 Initial dry mass ............................................................................................................................................. 12

8.2.1 Test product specimens ............................................................................................................................. 12

8.2.2 Virulence control specimens ................................................................................................................... 13

8.3 Sterilization of test specimens ................................................................................................................ 13

8.4 Preparation of the culture vessels ......................................................................................................... 13

8.5 Inoculation ...................................................................................................................................................... 13

8.6 Exposure of test specimens ...................................................................................................................... 13

8.6.1 Preparation of additive for white rot fungi ........................................................................................ 13

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SIST EN 113-3:2023
EN 113-3:2023 (E)

8.6.2 Test specimens .............................................................................................................................................. 14

8.6.3 Virulence control specimens .................................................................................................................... 14

8.6.4 Wetting check specimens .......................................................................................................................... 14

8.7 Culture conditions and duration of the test ........................................................................................ 14

8.8 Assessment of the test ................................................................................................................................. 14

8.8.1 Examination of the test specimens ......................................................................................................... 14

8.8.2 Final dry mass ................................................................................................................................................ 14

8.8.3 Validity of results .......................................................................................................................................... 15

9 Validity of the test ......................................................................................................................................... 15

10 Assessment of results .................................................................................................................................. 15

11 Test report ...................................................................................................................................................... 16

Annex A (informative) Guidance on sampling ................................................................................................ 18

Annex B (normative) Methods of sterilization ................................................................................................ 19

B.1 Ionizing radiation ......................................................................................................................................... 19

B.2 Steam ................................................................................................................................................................. 19

Annex C (informative) Culture vessels ............................................................................................................... 20

Annex D (informative) Example of a test report............................................................................................. 23

Annex E (informative) Test fungi ......................................................................................................................... 26

E.1 General information on maintaining and acquisition of test strains ........................................ 26

E.2 Maintenance and treatment of test fungi ............................................................................................. 26

E.3 Information regarding obligatory test fungi ...................................................................................... 27

E.3.1 Coniophora puteana (Schumacher ex Fries) Karsten [Synonym: Coniophora cerebella

(Persoon) Duby] ............................................................................................................................................ 27

E.3.2 Pleurotus ostreatus (Jacquin ex Fries) Kummer ................................................................................ 27

E.3.3 Gloeophyllum trabeum (Persoon ex Fries) Murrill [Synonyms: Lenzites trabea

(Persoon ex Fries) Fries - Trametes trabea (Persoon ex Fries) Bresadola] ............................ 27

E.3.4 Trametes versicolor (Linnaeus ex Fries) Pile [Synonyms: Polyporus versicolor

Linnaeus ex Fries - Polystictus versicolor (Linnaeus) Saccardo - Coriolus versicolor

(Linnaeus) Quelet] ....................................................................................................................................... 27

E.3.5 Rhodonia placenta (Fr.) Niemelä, K.H. Larss. and Schigel [Synonym: Poria placenta

(Fries) Cooke sensu J. Eriksson - Poria monticola Murrill] ............................................................ 28

E.4 Information regarding optional test fungi .......................................................................................... 28

Annex F (informative) Assessment of results .................................................................................................. 31

Annex G (informative) Moisture dynamics, coatings, composites and impact of dimensions....... 33

G.1 General information on moisture dynamics ....................................................................................... 33

G.2 Coated wood-based panels ........................................................................................................................ 33

G.3 Wood polymer composites and natural fibre composites ............................................................. 33

G.4 Impact of dimensions .................................................................................................................................. 34

Bibliography ................................................................................................................................................................. 35

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SIST EN 113-3:2023
EN 113-3:2023 (E)
European foreword

This document (EN 113-3:2023) has been prepared by Technical Committee CEN/TC 38 “Durability of

wood and wood-based products”, the secretariat of which is held by AFNOR.

This European Standard shall be given the status of a national standard, either by publication of an

identical text or by endorsement, at the latest by August 2023, and conflicting national standards shall be

withdrawn at the latest by August 2023.

Attention is drawn to the possibility that some of the elements of this document may be the subject of

patent rights. CEN shall not be held responsible for identifying any or all such patent rights.

This document supersedes ENV 12038:2002.
Test results obtained with earlier versions of ENV 12038 are still valid.
Compared to current EN 113, modifications are the following:
— A third part has been included.

— The three parts of the new EN 113 deal with similar testing but relate to a different scope.

EN 113-3:2023 includes the following significant technical changes with respect to ENV 12038:2002:

— Change of the title;
— The methods for sterilization are updated;
— All annexes are informative, except Annex B on sterilization methods;
— Some additional validity requirements are introduced for control specimens;
— Number of test specimens per fungus doubled;
— Number of virulence control specimens increased from 6 to 10;
— Size control specimens deleted;
— Assessment of results changed.

Any feedback and questions on this document should be directed to the users’ national standards body.

A complete listing of these bodies can be found on the CEN website.

According to the CEN-CENELEC Internal Regulations, the national standards organisations of the

following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,

Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,

Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North

Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United

Kingdom.
---------------------- Page: 6 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
Introduction

This document describes a laboratory test method in which small samples of the wood-based panel

product under test are exposed to attack by a range of wood-destroying basidiomycete fungi in pure

culture. The thickness of the test specimens varies, since it is dictated by the thickness of the wood-based

panel product under test. The effect of constituents giving temporary protection is avoided by testing

after pre-conditioning of the cut specimens in a freely ventilated environment. The test method also

includes a minimum moisture uptake requirement.

The procedures described in this document method are intended to be carried out by suitably trained

and/or supervised specialists. Appropriate safety precautions should be observed throughout the use of

this document.
---------------------- Page: 7 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
1 Scope

This document describes a method for assessing the durability of wood-based panels or analogue wood

products to attack by wood-destroying basidiomycete fungi growing in pure culture.

The test method described in this document is intended to complement EN 113-2 with focus on specific

aspects of wood-based panels or analogue wood products. This document is not intended to determine

the effectiveness of wood preservatives used to prevent decay, which is covered by EN 113-1.

NOTE This method can be used in conjunction with an appropriate ageing procedure, for example EN 73 or

EN 84.

The method is applicable to uncoated, rigid wood-based panel products. It is applicable to the

determination of the decay resistance of wood-based panel products:
— made from naturally durable materials;
— made from materials treated with preservatives prior to manufacture;

— treated with a preservative which is introduced during manufacture, for example as an additive to

the adhesive;

— specific treatments to increase durability of wood-based panels, e.g. wood modification.

Annex A (informative) contains a guidance on sampling.
Annex B (normative) contains some methods of sterilization.
Annex C (informative) contains information on the culture vessels.
Annex D (informative) contains an example of a test report.
Annex E (informative) contains information on the test fungi.
Annex F (informative) contains the assessment of the results.

Annex G (informative) contains extra info on moisture dynamics, coatings, composites and impact of

dimensions.
2 Normative references

The following documents are referred to in the text in such a way that some or all of their content

constitutes requirements of this document. For dated references, only the edition cited applies. For

undated references, the latest edition of the referenced document (including any amendments) applies.

EN 84, Durability of wood and wood-based products - Accelerated ageing of treated wood prior to biological

testing - Leaching procedure
---------------------- Page: 8 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

— IEC Electropedia: available at https://www.electropedia.org/
— ISO Online browsing platform: available at https://www.iso.org/obp
3.1
supplier

sponsor of the test, person or company providing the sample of wood/timber (wood-based panel) to be

tested
4 Principle

Specimens prepared from the wood-based panel product(s) under test, after pre-conditioning, and

control specimens of defined function are exposed to attack by pure cultures of wood-destroying

basidiomycete fungi.

After a prescribed period of incubation under defined conditions, the loss in dry mass of the specimens

is used as the criterion for determining the extent of attack.
5 Test material and apparatus
5.1 Biological material
5.1.1 Test fungi
5.1.1.1 General

The test fungi to be used are as follows and relate to corresponding virulence control specimens.

It is required that the reference timber virulence control specimens provide a median mass loss of at least

30 % with one of the test fungi.
5.1.1.2 Obligatory test fungi for all types of panel products
— Coniophora puteana (Schumacher ex Fries) Karsten (BAM Ebw. 15)

Loss in mass of Scots pine sapwood virulence control specimens in 16 weeks: minimum 20 % (m/m).

— Pleurotus ostreatus (Jacquin ex Fries) Kummer (FPRL 40C)
Loss in mass of beech virulence control specimens in 16 weeks: 20 % (m/m).
5.1.1.3 Species to be used compulsorily on the nature of the test product
For test products made only from softwood:
— Gloeophyllum trabeum (Persoon ex Fries) Murrill (BAM Ebw. 109)

Loss in mass of Scots pine sapwood virulence control specimens in 16 weeks: minimum 20 % (m/m).

---------------------- Page: 9 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
For test products made only from hardwood:
— Trametes versicolor (L.) Lloyd (CTB 863 A)

Loss in mass of beech virulence control specimens in 16 weeks: minimum 20 % (m/m).

For test products made from a mixture of softwood and hardwood, both Gloeophyllum trabeum and

Trametes versicolor shall be used.
5.1.1.4 Additional obligatory test fungus for modified wood
— Rhodonia placenta (Fr.) Niemelä, K.H. Larss. and Schigel (FPRL 280).
Loss in mass of Scots pine sapwood in 16 weeks: minimum 20 % (m/m).
5.1.1.5 Optional test fungi

For specific regional uses or conditions, it is also possible to choose other fungi on an optional basis .

5.1.1.6 Maintenance of fungal strains

The strains shall be maintained and treated (frequency of subculturing, alternation of culture media etc.)

in accordance with the instructions from their laboratory of origin (see Annex E). The parent strain shall

be maintained in the laboratory of its origin, so as to conserve and ensure its vigour.

If tests are not undertaken regularly, or if a strain shows signs of degeneration, a new standard culture of

the strain shall be obtained from the laboratory of origin for each test. When new strains are received,

the virulence shall be tested to ensure that the mass loss achieved is above the minimum value given in

Annex E.
5.1.2 Solid wood stock
5.1.2.1 Wood species
The following species shall be used for the test:
— Scots pine sapwood (Pinus sylvestris L.)
— European beech (Fagus sylvatica L.)
5.1.2.2 Quality of the wood

The wood shall be free from visible cracks, stain, decay, insect damage or other defects. The wood shall

not have been water-stored, floated, chemically treated or steamed.
NOTE Wood that has been kiln dried at temperatures not above 60 °C can be used.

The Scots pine shall be exclusively sapwood containing little resin and having between 2,5 and 8 annual

growth rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the

whole.

The beech shall be even grained, free from tyloses and discoloration. It shall have between 2 and 6 annual

growth rings per 10 mm.
1 Former name: Coriolus versicolor (Linnaeus) Quelet
See Annex E for a non-comprehensive list of recommended optional fungi.
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SIST EN 113-3:2023
EN 113-3:2023 (E)
5.1.2.3 Virulence control specimens

Prepare planed strips from the solid wood stock having a cross-section (25 ± 0,5) mm x (15 ± 0,5) mm.

The longitudinal faces shall be parallel to the direction of the grain. The annual rings shall not be parallel

to the faces (contact angle greater than 10°) but otherwise can run in any direction. Make transverse cuts,

neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to give virulence control

specimens (50 ± 0,5) mm long.

The dimensions of each virulence control specimen at a moisture content of (12 ± 2) % shall be

(50 ± 0,5) mm × (25 ± 0,5) mm × (15 ± 0,5) mm.

The specimens shall originate from a minimum of three trees or shall be taken from a stock originally of

more than 500 specimens.
5.2 Products and reagents
5.2.1 Culture medium
The culture medium shall be a malt agar medium with the following composition:

— malt extract: in concentrated form: (50 ± 0,5) g; in powder form: (40 ± 0,5) g;

— agar causing no inhibition of growth of fungi: (20 ± 0,5) g to (30 ± 0,5) g;
— de-ionized water; quantity to make up to 1 000 g.
— preferably use water conforming to grade 3 of EN ISO 3696.

Place in each culture vessel (5.3.4) a sufficient quantity of the medium to provide a minimum depth of

3 mm to 4 mm when in its in-use position. Close the vessels as specified in 5.3.4 and sterilize in an

autoclave at 121 °C for 20 min. Let the vessels cool in their in-use position.

NOTE The quantity of culture medium required in each culture vessel varies with the thickness of the test

product (see 8.4).
5.2.2 Additive for white rot fungi

Anhydrated, laminar, aluminium-iron-magnesium silicate exfoliated to yield particles up to 3 mm

diameter. Particles less than 2 mm diameter shall be removed by sieving. Before use, mix the sample of

additive well. The additive shall be used only once.

This ‘vermiculite’ additive is always required in combination with the test fungus Pleurotus ostreatus.

This ‘vermiculite’ additive is also required for Trametes versicolor when thicker panels (> 20 mm) are

under test.

A moisture meter of the two pronged electrical conductivity type is suitable for assessing moisture content, but

oven drying is used commonly to assess moisture content.
Vermiculite is suitable.
---------------------- Page: 11 ----------------------
SIST EN 113-3:2023
EN 113-3:2023 (E)
5.3 Apparatus
5.3.1 Conditioning supports

Made of glass, stainless steel or any other inert material, that is to say with no risk of having any effect on

the test specimens. The supports shall provide free circulation of air around the test specimens whilst

having a minimum of contact with the test specimens.
5.3.2 Conditioning room
Well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative humidity.
5.3.3 Culture chamber

Incubator or room, dark and controlled at (22 ± 2) °C and (70 ± 5) % relative humidity.

5.3.4 Culture vessels

Kolle flasks or equivalent vessels with a capacity of between 400 ml and 650 ml providing a flat surface

2 2

area of between 85 cm and 120 cm for the medium and close with a material that allows for air

exchange.
NOTE 1 Examples of suitable vessels are given in Annex C.

NOTE 2 Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with

leakproof lids, the centres of which are pierced with a round hole of up to 15 mm diameter and plugged with a wad

of cotton wool.
5.3.5 Ventilated drying oven
Capable of being controlled at (103 ± 2) °C.
5.3.6 Desiccators
With an efficient desiccant, for example silica gel.
5.3.7 Test specimens supports

Made of glass, stainless steel or any other inert material, that is to say with no risk of having any effect on

the culture medium, the fungus, the wood, or of modifying itself. The supports are used to prevent direct

contact of the specimens with the culture medium, but shall not separate them from it by more than 3 mm.

If abnormally high moisture contents are experienced consistently, use of specimen supports of

approximately 5 mm thick may help to control the problem. If thicker specimen supports are used, this

should be recorded in the test report. Abnormally high moistur
...

SLOVENSKI STANDARD
oSIST prEN 113-3:2021
01-november-2021
Trajnost lesa in lesnih proizvodov - Preskusna metoda proti glivam
prostotrosnicam, ki uničujejo les - 3. del: Ocenjevanje trajnosti lesenih plošč
Durability of wood and wood-based products - Test method against wood destroying
basidiomycetes - Part 3: Assessment of durability of wood-based panels
Dauerhaftigkeit von Holz und Holzprodukten - Prüfverfahren in Bezug auf Holz

zerstörende Basidiomyceten - Teil 3: Bewertung der Dauerhaftigkeit von Holzwerkstoffen

Durabilité du bois et des matériaux dérivés du bois - Méthode d’essai contre les

champignons basidiomycètes lignivores - Partie 3 : Évaluation de la durabilité des

panneaux à base de bois
Ta slovenski standard je istoveten z: prEN 113-3
ICS:
79.060.01 Lesne plošče na splošno Wood-based panels in
general
oSIST prEN 113-3:2021 en,fr,de

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------
oSIST prEN 113-3:2021
---------------------- Page: 2 ----------------------
oSIST prEN 113-3:2021
DRAFT
EUROPEAN STANDARD
prEN 113-3
NORME EUROPÉENNE
EUROPÄISCHE NORM
September 2021
ICS 79.060.01 Will supersede ENV 12038:2002
English Version
Durability of wood and wood-based products Test method
against wood destroying basidiomycetes - Part 3:
assessment of durability of wood-based panels

Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten -

Méthode d'essai contre les champignons Prüfverfahren in Bezug auf Holz zerstörende

basidiomycètes lignivores - Partie 3 : Évaluation de la Basidiomyceten - Teil 3: Bewertung der

durabilité des panneaux à base de bois Dauerhaftigkeit von Holzwerkstoffen

This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee

CEN/TC 38.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations

which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other

language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC

Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are

aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without

notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels

worldwide for CEN national Members.
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oSIST prEN 113-3:2021
prEN 113-3:2021 (E)
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