Water quality - General requirements and guidance for microbiological examinations by culture (ISO 8199:2018)

This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate.
This document is mainly intended for examinations for bacteria, yeasts and moulds, but some aspects are also applicable to bacteriophages, viruses and parasites. It excludes techniques not based on culturing microorganisms, such as polymerase chain reaction (PCR) methods.

Wasserbeschaffenheit - Allgemeine Anforderungen und Anleitung für mikrobiologische Untersuchungen mittels Kulturverfahren (ISO 8199:2018)

Dieses Dokument legt Anforderungen fest und gibt Anleitungen für die Durchführung von Arbeitsschritten, die allen Kulturtechniken zur mikrobiologischen Wasseruntersuchung gemeinsam sind, insbesondere die Vorbereitung von Proben, Nährmedien und den üblichen Geräten und Glasgeräten, sofern in den spezifischen Normen nicht anders gefordert. Dieses Dokument beschreibt auch die unterschiedlichen Kulturverfahren zum Nachweis und zur Zählung sowie die Auswahlkriterien für das am besten geeignete Verfahren.
Dieses Dokument ist hauptsächlich für Untersuchungen auf Bakterien, Hefen und Pilze bestimmt, einige Aspekte sind jedoch auch auf Bakteriophagen, Viren und Parasiten anwendbar. Ausgenommen sind Verfahren, die nicht auf der Kultivierung von Mikroorganismen beruhen, z. B. Methoden der Polymerase-Kettenreaktion (PCR).

Qualité de l'eau - Exigences et lignes directrices générales pour les examens microbiologiques sur milieu de culture (ISO 8199:2018)

Le présent document établit les exigences et fournit les lignes directrices sur les manipulations communes à chaque technique de culture pour l'examen microbiologique de l'eau, en particulier la préparation des échantillons, les milieux de culture ainsi que l'appareillage général et la verrerie, sauf autre exigence dans la norme spécifique. Il décrit également les diverses techniques disponibles pour la détection et le dénombrement sur milieu de culture et les critères permettant de déterminer la technique appropriée.
Le présent document concerne principalement les examens portant sur les bactéries, les levures et les moisissures, mais certains aspects peuvent aussi s'appliquer aux bactériophages, aux virus et aux parasites. Sont exclues les techniques ne reposant pas sur la culture de micro-organismes, telles que les méthodes de réaction en chaîne par polymérase (PCR).

Kakovost vode - Splošne zahteve in navodilo za mikrobiološke preiskave v kulturi (ISO 8199:2018)

Ta dokument določa zahteve in podaja navodilo za izvajanje manipulacij, ki so skupne vsaki tehniki kulture, za mikrobiološko preiskavo vode, zlasti za pripravo vzorcev, gojišča kultur ter splošnih aparatov in steklovine, razen če je v posebnem standardu zahtevano drugače. Opisuje tudi različne tehnike, ki so na voljo za ugotavljanje prisotnosti in števila v kulturi, ter merila za določitev ustrezne tehnike.
Ta dokument je namenjen predvsem za preiskave bakterij, plesni in kvasovk, nekateri vidiki pa se uporabljajo tudi za bakteriofage, viruse in parazite. V tem dokumentu niso zajete tehnike, ki ne temeljijo na gojenju mikroorganizmov, kot je verižna reakcija s polimerazo (PCR).

General Information

Status
Published
Publication Date
06-Nov-2018
Withdrawal Date
30-May-2019
Technical Committee
Drafting Committee
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
07-Nov-2018
Completion Date
07-Nov-2018

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 8199:2019
01-maj-2019
1DGRPHãþD
SIST EN ISO 8199:2007
Kakovost vode - Splošne zahteve in navodilo za mikrobiološke preiskave v kulturi
(ISO 8199:2018)
Water quality - General requirements and guidance for microbiological examinations by
culture (ISO 8199:2018)
Wasserbeschaffenheit - Allgemeine Anforderungen und Anleitung für mikrobiologische
Untersuchungen mittels Kulturverfahren (ISO 8199:2018)
Qualité de l'eau - Exigences et lignes directrices générales pour les examens
microbiologiques sur milieu de culture (ISO 8199:2018)
Ta slovenski standard je istoveten z: EN ISO 8199:2018
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
13.060.45 Preiskava vode na splošno Examination of water in
general
SIST EN ISO 8199:2019 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 8199:2019

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SIST EN ISO 8199:2019


EN ISO 8199
EUROPEAN STANDARD

NORME EUROPÉENNE

November 2018
EUROPÄISCHE NORM
ICS 07.100.20
English Version

Water quality - General requirements and guidance for
microbiological examinations by culture (ISO 8199:2018)
Qualité de l'eau - Exigences et lignes directrices Wasserbeschaffenheit - Allgemeine Anforderungen und
générales pour les examens microbiologiques sur Anleitung für mikrobiologische Untersuchungen
milieu de culture (ISO 8199:2018) mittels Kulturverfahren (ISO 8199:2018)
This European Standard was approved by CEN on 6 September 2018.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 8199:2018 E
worldwide for CEN national Members.

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SIST EN ISO 8199:2019
EN ISO 8199:2018 (E)
Contents Page
European foreword . 3

2

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SIST EN ISO 8199:2019
EN ISO 8199:2018 (E)
European foreword
This document (EN ISO 8199:2018) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2019, and conflicting national standards shall be
withdrawn at the latest by May 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 8199:2007.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 8199:2018 has been approved by CEN as EN ISO 8199:2018 without any modification.

3

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SIST EN ISO 8199:2019

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SIST EN ISO 8199:2019
INTERNA
...

SLOVENSKI STANDARD
oSIST prEN ISO 8199:2017
01-november-2017
.DNRYRVWYRGH6SORãQH]DKWHYHLQQDYRGLOR]DãWHWMHPLNURRUJDQL]PRYYJRMLãþX
,62',6
Water quality - General requirements and guidance for microbiological examinations by
culture (ISO/DIS 8199:2017)
Wasserbeschaffenheit - Allgemeine Anleitung zur Zählung von Mikroorganismen durch
Kulturverfahren (ISO/DIS 8199:2017)
Qualité de l'eau - Lignes directrices générales pour le dénombrement des micro-
organismes sur milieu de culture (ISO/DIS 8199:2017)
Ta slovenski standard je istoveten z: prEN ISO 8199
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
oSIST prEN ISO 8199:2017 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN ISO 8199:2017

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oSIST prEN ISO 8199:2017
DRAFT INTERNATIONAL STANDARD
ISO/DIS 8199
ISO/TC 147/SC 4 Secretariat: DIN
Voting begins on: Voting terminates on:
2017-10-04 2017-12-27
Water quality — General requirements and guidance for
microbiological examinations by culture
Titre manque
ICS: 07.100.20
THIS DOCUMENT IS A DRAFT CIRCULATED
This document is circulated as received from the committee secretariat.
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
ISO/CEN PARALLEL PROCESSING
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 8199:2017(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2017

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oSIST prEN ISO 8199:2017
ISO/DIS 8199:2017(E)

COPYRIGHT PROTECTED DOCUMENT
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
the requester.
ISO copyright office
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CH-1214 Vernier, Geneva, Switzerland
Tel. +41 22 749 01 11
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copyright@iso.org
www.iso.org
ii © ISO 2017 – All rights reserved

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oSIST prEN ISO 8199:2017
ISO/DIS 8199:2017(E)
Contents Page
Foreword . iv
Introduction . v
1 Scope .1
2 Normative references .1
3 Terms and definitions .1
4 Principle .4
5 General measurement requirements .4
6 Diluents and culture media .5
7 Sterilization and decontamination.5
8 Samples and sample handling .7
9 Enumeration (quantitative) methods .8
10 Detection (qualitative) methods . 32
11 Performance characteristics of methods . 33
12 Analytical quality control .
...

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