EN 17359:2020

Stationary source emissions - Bioaerosols and biological agents - Sampling of bioaerosols and collection in liquids - Impingement method

This document contains specifications for active sampling of bioaerosols from exhaust air flowing through a defined cross-section of a stack. It defines general principles that have to be taken into account during an isokinetic sampling campaign for bioaerosols by bubbling the exhaust air through a specific impinger designed for emission measurements.
In this document the application with culturable organisms is specified but the same principle might be applicable for non-cultural based methods (e.g. molecular and/or enzyme-based methods).
The impinger is designed to allow a sample volume flow of 1 m3/h to 1,8 m3/h, or 16 l/min to 30 l/min, respectively, and has been tested with regard to various microorganisms within broad concentration ranges [1; 2; 3; 4]

Emissionen aus stationären Quellen - Bioaerosole und biologische Agenzien - Probenahme von Bioaerosolen und Abscheidung in Flüssigkeiten - Impinger-Methode

Dieses Dokument enthält Festlegungen zur aktiven Probenahme von Bioaerosolen aus der Abluft, die durch einen definierten Querschnitt eines Abluftkanals strömt. Sie legt allgemeine Grundlagen fest, die bei einer isokinetischen Probenahmekampagne für Bioaerosole berücksichtigt werden müssen, wobei die Abluft durch einen spezifischen Impinger, der auf Emissionsmessungen ausgelegt ist, geleitet wird.
In diesem Dokument wird die Anwendung mit anzüchtbaren Organismen festgelegt, jedoch kann dasselbe Prinzip auch auf Verfahren angewendet werden, die nicht auf Kulturen basieren (z. B. molekulare und/oder enzymbasierte Verfahren).
Der Impinger ist auf einen Teilvolumenstrom von 1 m3/h bis 1,8 m3/h bzw. 16 l/min bis 30 l/min ausgelegt, und wurde für verschiedene Mikroorganismen in breiten Konzentrationsbereichen geprüft [1; 2; 3; 4].

Émissions de sources fixes - Bioaérosols et agents biologiques - Prélèvement des bioaérosols et collecte dans les liquides - Méthode d’impaction par bullage

Le présent document contient des spécifications relatives au prélèvement actif des bioaérosols présents dans l’air rejeté à travers une section définie d’une cheminée. Il définit des principes généraux qui doivent être pris en compte au cours d’une campagne de prélèvement isocinétique de bioaérosols, en faisant passer des bulles d’air rejeté à travers un bulleur spécifiquement conçu pour les mesurages d’émissions.
Bien que le présent document spécifie l’utilisation d’organismes cultivables, le même principe peut s’appliquer aux méthodes hors cultures (méthodes moléculaires et/ou de type enzymatique, par exemple).
Le bulleur est conçu pour obtenir un débit volumique d’échantillon de 1 m3/h à 1,8 m3/h, soit de 16 l/min à 30 l/min, et a été soumis à essai avec divers micro-organismes sur des gammes de concentrations étendues [1 ; 2 ; 3 ; 4].

Emisije nepremičnih virov - Bioaerosoli in biološki agensi - Vzorčenje bioaerosolov in zajem v tekočini - Metoda z izpiranjem

General Information

• Status: Published
• Publication Date: 11-Aug-2020
• Withdrawal Date: 27-Feb-2021
• ICS: 13.040.20 - Ambient atmospheres
• Technical Committee: CEN/TC 264 - Air quality
• Drafting Committee: CEN/TC 264/WG 28 - Measurement of airborne microorganisms in ambient air
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 12-Aug-2020
• Due Date: 22-Dec-2020
• Completion Date: 12-Aug-2020
• Ref Project: SIST EN 17359:2020 - Stationary source emissions - Bioaerosols and biological agents - Sampling of bioaerosols and collection in liquids - Impingement method

Standards Content (Sample)

SLOVENSKI STANDARD
01-oktober-2020
Emisije nepremičnih virov - Bioaerosoli in biološki agensi - Vzorčenje
bioaerosolov in zajem v tekočini - Metoda z izpiranjem
Stationary source emissions - Bioaerosols and biological agents - Sampling of
bioaerosols and collection in liquids - Impingement method
Emissionen aus stationären Quellen - Bioaerosole und biologische Agenzien -
Probenahme von Bioaerosolen und Abscheidung in Flüssigkeiten - Impinger-Methode
Émissions de sources fixes - Bioaérosols et agents biologiques - Prélèvement des
bioaérosols et collecte dans les liquides - Méthode d’impaction par bullage
Ta slovenski standard je istoveten z: EN 17359:2020
ICS:
13.040.40 Emisije nepremičnih virov Stationary source emissions
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 17359
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2020
EUROPÄISCHE NORM
ICS 13.040.20
English Version
Stationary source emissions - Bioaerosols and biological
agents - Sampling of bioaerosols and collection in liquids -
Impingement method
Émissions de sources fixes - Bioaérosols et agents Emissionen aus stationären Quellen - Bioaerosole und
biologiques - Prélèvement des bioaérosols et collecte biologische Agenzien - Probenahme von Bioaerosolen
dans les liquides - Méthode d'impaction par bullage und Abscheidung in Flüssigkeiten - Impinger-Methode
This European Standard was approved by CEN on 5 July 2020.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2020 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 17359:2020 E
worldwide for CEN national Members.

Contents Page
European foreword . 5
1 Scope . 6
2 Normative references . 6
3 Terms and definitions . 7
4 Symbols and abbreviations . 11
5 Principle of method . 13
6 Theoretical fundamentals . 14
6.1 Isokinetic sampling . 14
6.2 Determination of concentration and load of the microorganisms . 14
7 Device and materials . 16
7.1 General . 16
7.2 Device and methods for measurement of the exhaust air parameters for the
calculation of the main volume flow . 16
7.2.1 General . 16
7.2.2 Device for determination of the exhaust air velocity . 16
7.2.3 Device for determination of pressure, temperature and humidity . 17
7.3 Device for the sampling of bioaerosols . 17
7.3.1 General . 17
7.3.2 Material properties . 18
7.3.3 Entry nozzle, bend and sampling probe . 18
7.3.4 Emission impinger . 18
7.3.5 Suction device and device for measurement of the gas volume or respectively the gas
volume flow . 20
8 Sampling . 20
8.1 General . 20
8.2 Preparation of the sampling equipment . 20
8.2.1 General . 20
8.2.2 Preparation of the emission impinger . 20
8.2.3 Preparation of the entry nozzle and the sampling probe . 21
8.2.4 Determination of appropriate sampling probe and sampling flow . 21
8.3 Performing bioaerosol sampling . 24
8.3.1 Leak test and sampling . 24
8.3.2 Recovery of deposits upstream of the emission impinger . 25
8.3.3 Determination of the mass of the sampling liquid . 26
8.3.4 Field blank value . 26
8.3.5 Analytical blank value . 27
8.4 Transport and storage . 27
9 Analysis . 27
10 Evaluation . 27
10.1 General . 27
10.2 Transfer of the results by the analytical laboratory . 28
10.3 Sample gas volume during sampling. 28
10.4 Microorganism number calculation . 30
10.5 Load calculation . 31
11 Performance characteristerics . 31
11.1 Measurement uncertainty . 31
11.2 Parameters for the determination of measuring uncertainity in practice . 32
12 Maintenance and quality assurance . 37
13 Sampling efficiency and limits of the method . 37
14 Interferences . 38
Annex A (informative) Practical example for moulds and bacteria . 39
A.1 General . 39
A.2 Determination of the measurement points . 39
A.3 Devices and materials . 39
A.3.1 General . 39
A.3.2 Devices and methods for measurement of the exhaust air parameters for the
calculation of the main volume flow . 39
A.3.2.1 General . 39
A.3.2.2 Devices for determination of the exhaust air velocity . 39
A.3.2.3 Devices for determination of pressure, temperature and humidity. 40
A.3.3 Devices for sampling of bioaerosols . 40
A.3.3.1 General . 40
A.3.3.2 Material properties . 40
A.3.3.3 Entry nozzle, bend and sampling probe . 40
A.3.3.4 Emission impinger . 40
A.3.3.5 Measurement system for isokinetic sample volume flow abstraction . 40
A.4 Sampling process . 40
A.4.1 General . 40
A.4.2 Preparation of the sampling equipment . 40
A.4.2.1 General . 40
A.4.2.2 Preparation of the emission impinger . 41
A.4.2.3 Preparation of the entry nozzle, band and sampling probe . 41
A.4.3 Measurement of the exhaust air parameters for isokinetic sampling . 41
A.4.4 Sampling . 42
A.4.4.1 General . 42
A.4.4.2 Recovery of deposits upstream of the emission impinger . 42
A.4.4.3 Field blank value . 42
A.4.5 Transport and storage .
...