prEN ISO 18363-2
(Main)Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 2: Method using slow alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO/FDIS 18363-2:2024)
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 2: Method using slow alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO/FDIS 18363-2:2024)
This document specifies a procedure for the parallel determination of glycidol together with 2-MCPD and 3-MCPD present in bound or free form in oils and fats. The method is based on alkaline-catalysed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derivatisation of the derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). Though free MCPD and glycidol are supposed to be present in fats and oils in low to negligible quantities only, in the event that free analytes are present, they would contribute proportionately to the results. The results always being the sum of the free and the bound form of a single analyte.
This method is applicable to solid and liquid fats and oils. This document can also apply to animal fats and used frying oils and fats, but a validation study is undertaken before the analysis of these matrices.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenem Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 2: Verfahren mittels langsamer alkalischer Umesterung und Messung für 2-MCPD, 3-MCPD und Glycidol
Corps gras d'origines animale et végétale - Détermination des esters de chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras par CPG/SM - Partie 2: Méthode par transestérification alcaline lente et mesure pour le 2-MCPD, le 3-MCPD et le glycidol (ISO/FDIS 18363-2:2024)
Le présent document décrit un mode opératoire permettant la détermination parallèle du glycidol ainsi que du 2-MCPD et du 3-MCPD présents sous forme liée ou libre dans les corps gras. La méthode repose sur un clivage des esters par catalyse alcaline, une transformation du glycidol ainsi libéré en monobromopropanediol (MBPD) et une dérivatisation des diols libres (MCPD et MBPD) en présence d'acide phénylboronique (PBA). Même si les corps gras ne sont censés contenir que des quantités faibles voire négligeables de MCPD libre et de glycidol libre, une teneur importante augmenterait proportionnellement la détermination des analytes liés.
La présente méthode est applicable aux corps gras solides et liquides. Le présent document peut également s'appliquer aux graisses animales et aux huiles de friture usagées, mais une étude de validation est menée avant de procéder à l'analyse de ces matrices.
Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) sont exclus du domaine d'application du présent document.
Živalske in rastlinske maščobe in olja - Ugotavljanje na maščobno kislino vezanih kloropropanediolov (MCPD) in glicidola z GC/MS - 2. del: Metoda z uporabo počasnega alkalnega preestrenja in meritev 2-MCPD, 3-MCPD in glicidola (ISO/FDIS 18363-2:2024)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-oktober-2024
Živalske in rastlinske maščobe in olja - Ugotavljanje na maščobno kislino vezanih
kloropropanediolov (MCPD) in glicidola z GC/MS - 2. del: Metoda z uporabo
počasnega alkalnega preestrenja in meritev 2-MCPD, 3-MCPD in glicidola
(ISO/FDIS 18363-2:2024)
Animal and vegetable fats and oils - Determination of fatty-acid-bound
chloropropanediols (MCPDs) and glycidol by GC/MS - Part 2: Method using slow alkaline
transesterification and measurement for 2-MCPD, 3-MCPD and glycidol (ISO/FDIS
18363-2:2024)
Tierische und pflanzliche Fette und Öle - Bestimmung von fettsäuregebundenem
Chlorpropandiol (MCPD) und Glycidol mittels GC/MS - Teil 2: Verfahren mittels
langsamer alkalischer Umesterung und Messung für 2-MCPD, 3-MCPD und Glycidol
Corps gras d'origines animale et végétale - Détermination des esters de
chloropropanediols (MCPD) et d'acides gras et des esters de glycidol et d'acides gras
par CPG/SM - Partie 2: Méthode par transestérification alcaline lente et mesure pour le 2
-MCPD, le 3-MCPD et le glycidol (ISO/FDIS 18363-2:2024)
Ta slovenski standard je istoveten z: prEN ISO 18363-2
ICS:
67.200.10 Rastlinske in živalske Animal and vegetable fats
maščobe in olja and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
FINAL DRAFT
International
Standard
ISO/FDIS 18363-2
ISO/TC 34/SC 11
Animal and vegetable fats and
Secretariat: BSI
oils — Determination of fatty-acid-
Voting begins on:
bound chloropropanediols (MCPDs)
2024-08-28
and glycidol by GC/MS —
Voting terminates on:
2024-11-20
Part 2:
Method using slow alkaline
transesterification and
measurement for 2-MCPD, 3-MCPD
and glycidol
Corps gras d'origines animale et végétale — Détermination des
esters de chloropropanediols (MCPD) et d'acides gras et des
esters de glycidol et d'acides gras par CPG/SM —
Partie 2: Méthode par transestérification alcaline lente et mesure
pour le 2-MCPD, le 3-MCPD et le glycidol
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
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TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 18363-2:2024(en) © ISO 2024
FINAL DRAFT
ISO/FDIS 18363-2:2024(en)
International
Standard
ISO/FDIS 18363-2
ISO/TC 34/SC 11
Animal and vegetable fats and
Secretariat: BSI
oils — Determination of fatty-acid-
Voting begins on:
bound chloropropanediols (MCPDs)
and glycidol by GC/MS —
Voting terminates on:
Part 2:
Method using slow alkaline
transesterification and
measurement for 2-MCPD, 3-MCPD
and glycidol
Corps gras d'origines animale et végétale — Détermination des
esters de chloropropanediols (MCPD) et d'acides gras et des
esters de glycidol et d'acides gras par CPG/SM —
Partie 2: Méthode par transestérification alcaline lente et mesure
pour le 2-MCPD, le 3-MCPD et le glycidol
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2024
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
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MADE IN NATIONAL REGULATIONS.
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Published in Switzerland Reference number
ISO/FDIS 18363-2:2024(en) © ISO 2024
ii
ISO/FDIS 18363-2:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
5.1 General .3
5.2 Solvents and chemicals .3
5.3 Standard and reference compounds .3
5.4 Working solutions** .4
5.5 Other solutions .4
6 Apparatus . 4
7 Sample . 5
7.1 Sampling .5
7.2 Preparation of the test sample .5
8 Procedure . 5
8.1 Spiking with surrogate standard and homogenization .5
8.2 Ester cleavage and glycidol transformation .5
8.3 Matrix removal .6
8.4 Derivatization .6
8.5 Gas chromatography/mass spectrometry references .6
9 Expression of results . 7
9.1 Determination of bound glycidol . .7
9.2 Determination of bound 2-MCPD .8
9.3 Determination of bound 3-MCPD .9
9.4 Determination of the degree of diester cleavage .9
9.5 Quality control .9
10 Notes . . 10
Annex A (informative) Examples of relevant chromatograms and data evaluation using “low-
MCPD” palm oil .12
Annex B (informative) Results of interlaboratory tests . 19
Bibliography .21
iii
ISO/FDIS 18363-2:2024(en)
Foreword
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This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11,
Animal and vegetable fats and oils, in collaboration with the European Committee for Standardization (CEN)
Technical Committee CEN/TC 307, Oilseeds, vegetable and animal fats and oils and their by-products - Methods
of sampling and analysis, in accordance with the Agreement on technical cooperation between ISO and CEN
(Vienna Agreement).
This second edition cancels and replaces the first edition (ISO 18363-2:2018), of which it constitutes a minor
revision to align the Introduction with ISO 18363-4:2021.
A list of all parts in the ISO 18363 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found a
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