EN ISO 10705-2:2001

Water quality - Detection and enumeration of bacteriophages - Part 2: Enumeration of somatic coliphages (ISO 10705-2:2000)

Wasserbeschaffenheit - Nachweis und Zählung von Bakteriophagen - Teil 2: Zählung von somatischen Coliphagen (ISO 10705-2:2000)

Dieser Teil von ISO 10705 legt ein Verfahren zum Nachweis und zur Zählung
von somatischen Coliphagen durch Bebrütung der Probe mit einem geeigneten
Wirtsstamm fest. Das Verfahren kann für alle Arten von Wasser, Sedimenten und
Schlämmen angewandt werden, wenn notwendig nach Verdünnung. Das Ver-fahren
kann auch für Schalentierextrakte angewandt werden.
Bei geringer Phagenanzahl kann eine Aufkonzentration notwendig sein, für die
eine gesonderte Internationale Norm entwickelt werden wird.
ANMERKUNG Es ist wünschenswert, dass so weit wie möglich nach Internationalen Normen
verfahren wird. Dieser Teil von ISO 10705 beinhaltet einen Verweis auf alternative Verfahren, die
keine teuren Materialien oder Zubehör erfordern, welche in Entwicklungsländern möglicherweise
nicht ohne weiteres verfügbar sind. Die Anwendung dieser Alternativen hat keinen Einfluss auf
die Durchführung dieses Verfahrens.

Qualité de l'eau - Détection et dénombrement des bactériophages - Partie 2: Dénombrement des coliphages somatiques (ISO 10705-2:2000)

La présente partie de l'ISO 10705 spécifie une méthode de détection et de dénombrement des coliphages somatiques par incubation de l'échantillon avec une souche-hôte appropriée. La méthode est applicable à tous les types d'eaux, aux extraits de sédiments et de boues, si nécessaire après dilution. La méthode est également applicable aux extraits de coquillages.  Dans le cas d'une faible population de phages, une étape de préconcentration peut s'avérer nécessaire pour laquelle une Norme internationale distincte sera élaborée ultérieurement.  
NOTE Il est souhaitable que les Normes Internationales soient adoptées le plus largement possible. La présente partie de l'ISO 10705 comporte des références à des procédures alternatives qui parent à la nécessité d'utiliser du matériel ou des équipements coûteux qui peuvent ne pas être aisément disponibles dans les pays en développement. L'utilisation de ces procédures alternatives n'affecte pas les performances de la présente méthode.

Kakovost vode - Ugotavljanje prisotnosti in števila bakteriofagov - 2. del - Ugotavljanje števila somatskih kolifagov (ISO 10705-2:2000)

General Information

• Status: Published
• Publication Date: 14-Aug-2001
• Withdrawal Date: 27-Feb-2002
• ICS: 07.100.20 - Microbiology of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Start Date: 21-May-2008
• Completion Date: 21-May-2008
• Ref Project: SIST EN ISO 10705-2:2001 - Water quality - Detection and enumeration of bacteriophages - Part 2: Enumeration of somatic coliphages (ISO 10705-2:2000)

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2001
1DGRPHãþD
SIST ISO 10705-2:2000
Kakovost vode - Ugotavljanje prisotnosti in števila bakteriofagov - 2. del -
Ugotavljanje števila somatskih kolifagov (ISO 10705-2:2000)
Water quality - Detection and enumeration of bacteriophages - Part 2: Enumeration of
somatic coliphages (ISO 10705-2:2000)
Wasserbeschaffenheit - Nachweis und Zählung von Bakteriophagen - Teil 2: Zählung
von somatischen Coliphagen (ISO 10705-2:2000)
Qualité de l'eau - Détection et dénombrement des bactériophages - Partie 2:
Dénombrement des coliphages somatiques (ISO 10705-2:2000)
Ta slovenski standard je istoveten z: EN ISO 10705-2:2001
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 10705-2
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2001
ICS 07.100.20
English version
Water quality - Detection and enumeration of bacteriophages -
Part 2: Enumeration of somatic coliphages (ISO 10705-2:2000)
Qualité de l'eau - Détection et dénombrement des Wasserbeschaffenheit - Nachweis und Zählung von
bactériophages - Partie 2: Dénombrement des coliphages Bakteriophagen - Teil 2: Zählung von somatischen
somatiques (ISO 10705-2:2000) Coliphagen (ISO 10705-2:2000)
This European Standard was approved by CEN on 22 June 2001.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10705-2:2001 E
worldwide for CEN national Members.

CORRECTED 2001-11-07
Foreword
The text of the International Standard from Technical Committee ISO/TC 147 "Water quality"
of the International Organization for Standardization (ISO) has been taken over as an
European Standard by Technical Committee CEN/TC 230 "Water analysis", the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication
of an identical text or by endorsement, at the latest by February 2002, and conflicting national
standards shall be withdrawn at the latest by February 2002.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy,
Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United
Kingdom.
Endorsement notice
The text of the International Standard ISO 10705-2:2000 has been approved by CEN as a
European Standard without any modifications.
NOTE: Normative references to International Standards are listed in annex ZA (normative).
Annex ZA (normative)
Normative references to international publications
with their relevant European publications
This European Standard incorporates by dated or undated reference, provisions from other
publications. These normative references are cited at the appropriate places in the text and the
publications are listed hereafter. For dated references, subsequent amendments to or revisions
of any of these publications apply to this European Standard only when incorporated in it by
amendment or revision. For undated references the latest edition of the publication referred to
applies (including amendments).
NOTE Where an International Publication has been modified by common modifications,
indicated by (mod.), the relevant EN/HD applies.
Publication Year Title EN Year
ISO 3696 1987 Water for analytical laboratory use - EN ISO 3696 1995
Specification and test methods
ISO 5667-1 1980 Water quality — Sampling — Part 1: EN 25667-1 1993
Guidance on the design of sampling
programmes
ISO 5667-2 1991 Water quality — Sampling — Part 2: EN 25667-2 1993
Guidance on sampling techniques
ISO 5667-3 1994 Water quality - Sampling - Part 3: EN ISO 5667-3 1995
Guidance on the preservation and
handling of samples
INTERNATIONAL ISO
STANDARD 10705-2
First edition
2000-04-01
Water quality — Detection and enumeration
of bacteriophages —
Part 2:
Enumeration of somatic coliphages
Qualité de l'eau — Détection et dénombrement des bactériophages —
Partie 2: Dénombrement des coliphages somatiques
Reference number
ISO 10705-2:2000(E)
©
ISO 2000
ISO 10705-2:2000(E)
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ii © ISO 2000 – All rights reserved

ISO 10705-2:2000(E)
Contents Page
1 Scope .1
2 Normative references .1
3 Terms and definitions .1
4 Safety precautions.2
5 Principle.2
6 Diluent, culture media and reagents.2
6.1 Basic materials.2
6.2 Diluent.2
7 Apparatus and glassware .2
8 Microbiological reference cultures .4
9 Sampling.4
10 Preparation of test material .5
10.1 Culturing and maintenance of host strains .5
10.2 Calibration of absorbance measurements for counts of viable microorganisms .6
11 Procedure .6
11.1 Preparation of inoculum cultures .6
11.2 Standard procedure.7
11.3 Method for samples with high bacterial background flora .7
11.4 Samples with low phage counts .7
11.5 Presence/absence test .8
11.6 Quality assurance.8
12 Expression of results .9
12.1 Plaque count procedures (11.2 to 11.4).9
12.2 Presence/absence test (11.5).9
13 Test report .10
Annex A (normative) Culture media, reagents and diluent.11
Annex B (informative) General description of somatic bacteriophages .14
Annex C (informative) Culturing of bacteriophage ��X174.15
��
Bibliography.16
ISO 10705-2:2000(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO
member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this part of ISO 10705 may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 10705-2 was prepared by Technical Committee ISO/TC 147, Water quality,
Subcommittee SC 4, Microbiological methods.
ISO 10705 consists of the following parts, under the general title Water quality — Detection and enumeration of
bacteriophages:
� Part 1: Enumeration of F-specific RNA bacteriophages
� Part 2: Enumeration of somatic coliphages
� Part 3: Concentration methods
� Part 4: Enumeration of bacteriophages infecting Bacteroides fragilis
Annex A forms a normative part of this part of ISO 10705. Annexes B and C are for information only.
iv © ISO 2000 – All rights reserved

INTERNATIONAL STANDARD ISO 10705-2:2000(E)
Water quality — Detection and enumeration of bacteriophages —
Part 2:
Enumeration of somatic coliphages
1 Scope
This part of ISO 10705 specifies a method for the detection and enumeration of somatic coliphages by incubating the
sample with an appropriate host strain. The method is applicable to all kinds of water, sediments and sludge extracts,
where necessary after dilution. The method is also applicable to shellfish extracts.
In the case of low phage numbers, a preconcentration step may be necessary for which a separate International
Standard will be developed.
NOTE It is desirable for International Standards to be adopted as widely as possible. This part of ISO 10705 includes
reference to alternative procedures which obviate the need for expensive materials or equipment which may not be readily
available in developing countries. Use of these alternatives will not affect the performance of this method.
2 Normative
...