EN ISO 8199:2018
(Main)Water quality - General requirements and guidance for microbiological examinations by culture (ISO 8199:2018)
Water quality - General requirements and guidance for microbiological examinations by culture (ISO 8199:2018)
This document specifies requirements and gives guidance for performing the manipulations common to each culture technique for the microbiological examination of water, particularly the preparation of samples, culture media, and general apparatus and glassware, unless otherwise required in the specific standard. It also describes the various techniques available for detection and enumeration by culture and the criteria for determining which technique is appropriate.
This document is mainly intended for examinations for bacteria, yeasts and moulds, but some aspects are also applicable to bacteriophages, viruses and parasites. It excludes techniques not based on culturing microorganisms, such as polymerase chain reaction (PCR) methods.
Wasserbeschaffenheit - Allgemeine Anforderungen und Anleitung für mikrobiologische Untersuchungen mittels Kulturverfahren (ISO 8199:2018)
Dieses Dokument legt Anforderungen fest und gibt Anleitungen für die Durchführung von Arbeitsschritten, die allen Kulturtechniken zur mikrobiologischen Wasseruntersuchung gemeinsam sind, insbesondere die Vorbereitung von Proben, Nährmedien und den üblichen Geräten und Glasgeräten, sofern in den spezifischen Normen nicht anders gefordert. Dieses Dokument beschreibt auch die unterschiedlichen Kulturverfahren zum Nachweis und zur Zählung sowie die Auswahlkriterien für das am besten geeignete Verfahren.
Dieses Dokument ist hauptsächlich für Untersuchungen auf Bakterien, Hefen und Pilze bestimmt, einige Aspekte sind jedoch auch auf Bakteriophagen, Viren und Parasiten anwendbar. Ausgenommen sind Verfahren, die nicht auf der Kultivierung von Mikroorganismen beruhen, z. B. Methoden der Polymerase-Kettenreaktion (PCR).
Qualité de l'eau - Exigences et lignes directrices générales pour les examens microbiologiques sur milieu de culture (ISO 8199:2018)
Le présent document établit les exigences et fournit les lignes directrices sur les manipulations communes à chaque technique de culture pour l'examen microbiologique de l'eau, en particulier la préparation des échantillons, les milieux de culture ainsi que l'appareillage général et la verrerie, sauf autre exigence dans la norme spécifique. Il décrit également les diverses techniques disponibles pour la détection et le dénombrement sur milieu de culture et les critères permettant de déterminer la technique appropriée.
Le présent document concerne principalement les examens portant sur les bactéries, les levures et les moisissures, mais certains aspects peuvent aussi s'appliquer aux bactériophages, aux virus et aux parasites. Sont exclues les techniques ne reposant pas sur la culture de micro-organismes, telles que les méthodes de réaction en chaîne par polymérase (PCR).
Kakovost vode - Splošne zahteve in navodilo za mikrobiološke preiskave v kulturi (ISO 8199:2018)
Ta dokument določa zahteve in podaja navodilo za izvajanje manipulacij, ki so skupne vsaki tehniki kulture, za mikrobiološko preiskavo vode, zlasti za pripravo vzorcev, gojišča kultur ter splošnih aparatov in steklovine, razen če je v posebnem standardu zahtevano drugače. Opisuje tudi različne tehnike, ki so na voljo za ugotavljanje prisotnosti in števila v kulturi, ter merila za določitev ustrezne tehnike.
Ta dokument je namenjen predvsem za preiskave bakterij, plesni in kvasovk, nekateri vidiki pa se uporabljajo tudi za bakteriofage, viruse in parazite. V tem dokumentu niso zajete tehnike, ki ne temeljijo na gojenju mikroorganizmov, kot je verižna reakcija s polimerazo (PCR).
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-maj-2019
1DGRPHãþD
SIST EN ISO 8199:2007
Kakovost vode - Splošne zahteve in navodilo za mikrobiološke preiskave v kulturi
(ISO 8199:2018)
Water quality - General requirements and guidance for microbiological examinations by
culture (ISO 8199:2018)
Wasserbeschaffenheit - Allgemeine Anforderungen und Anleitung für mikrobiologische
Untersuchungen mittels Kulturverfahren (ISO 8199:2018)
Qualité de l'eau - Exigences et lignes directrices générales pour les examens
microbiologiques sur milieu de culture (ISO 8199:2018)
Ta slovenski standard je istoveten z: EN ISO 8199:2018
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
13.060.45 Preiskava vode na splošno Examination of water in
general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 8199
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2018
EUROPÄISCHE NORM
ICS 07.100.20
English Version
Water quality - General requirements and guidance for
microbiological examinations by culture (ISO 8199:2018)
Qualité de l'eau - Exigences et lignes directrices Wasserbeschaffenheit - Allgemeine Anforderungen und
générales pour les examens microbiologiques sur Anleitung für mikrobiologische Untersuchungen
milieu de culture (ISO 8199:2018) mittels Kulturverfahren (ISO 8199:2018)
This European Standard was approved by CEN on 6 September 2018.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 8199:2018 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 8199:2018) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2019, and conflicting national standards shall be
withdrawn at the latest by May 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 8199:2007.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 8199:2018 has been approved by CEN as EN ISO 8199:2018 without any modification.
INTERNATIONAL ISO
STANDARD 8199
Third edition
2018-10
Water quality — General requirements
and guidance for microbiological
examinations by culture
Qualité de l’eau — Exigences et lignes directrices générales pour les
examens microbiologiques sur milieu de culture
Reference number
ISO 8199:2018(E)
©
ISO 2018
ISO 8199:2018(E)
© ISO 2018
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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ii © ISO 2018 – All rights reserved
ISO 8199:2018(E)
Contents Page
Foreword .v
Introduction .vii
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 4
5 General measurement requirements . 4
5.1 Uniformity of temperatures . 4
5.2 Incubation times . 4
5.3 Volumes and masses . 4
6 Diluents and culture media . 5
6.1 General . 5
6.2 Quality requirements of ingredients . 5
6.3 Diluents . 5
7 Sterilization and decontamination . 5
7.1 Sterilization of apparatus and glassware . 5
7.2 Sterilization of consumables . 6
7.3 Decontamination of glassware and materials after use . 6
7.4 Waste management . 6
8 Samples and sample handling . 6
8.1 Sampling . 6
8.2 Sample preparation . 7
8.2.1 Waters and other aqueous matrices . 7
8.2.2 Swabs . 7
9 Enumeration (quantitative) methods . 8
9.1 Inoculation of test portions in (or on) solid media . 8
9.1.1 General. 8
9.1.2 Pour plate technique . 8
9.1.3 Spread plate technique . 9
9.1.4 Membrane filtration technique .10
9.1.5 Incubation .12
9.1.6 Counting and confirmation from solid media .12
9.1.7 General guidance for calculation of results . .13
9.1.8 Expression of results .14
9.2 Enumeration using a liquid medium.24
9.2.1 General.24
9.2.2 Procedure .25
9.2.3 Choice of inoculation system .25
9.2.4 Incubation .26
9.2.5 Interpretation of results .26
9.2.6 Uncertainty of test results .27
9.2.7 Determination of MPN values .27
10 Detection (qualitative) methods .30
10.1 General .30
10.2 Procedure .31
10.3 Uncertainty of test results .31
11 Performance characteristics of methods .
...
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