CEN/TS 15083-1:2005
(Main)Durability of wood and wood-based products - Determination of the natural durability of solid wood against wood-destroying fungi, test methods - Part 1: Basidiomycetes
Durability of wood and wood-based products - Determination of the natural durability of solid wood against wood-destroying fungi, test methods - Part 1: Basidiomycetes
This CEN Technical Specification specifies a method of test for determining the natural durability of a timber against wood destroying basidiomycetes cultured on an agar medium. The method is applicable to all timber species.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73 or EN 84.
Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung der natürlichen Dauerhaftigkeit von Vollholz gegen holzzerstörende Pilze, Prüfverfahren - Teil 1: Basidiomyceten
Diese Technische Spezifikation legt ein Prüfverfahren zur Bestimmung der natürlichen Dauerhaftigkeit einer Holzart gegen holzzerstörende Basidiomyceten fest, die auf einem Agar Medium gezüchtet wurden. Das Verfahren ist auf alle Holzarten anwendbar.
ANMERKUNG Dieses Verfahren kann in Zusammenhang mit einer Alterungsbeanspruchung angewendet werden, z. B. nach EN 73 oder EN 84.
Durabilité du bois et des matériaux dérivés du bois - Détermination de la durabilité naturelle du bois massif vis-à-vis des champignons lignivores, méthodes d'essai - Partie 1: Basidiomycètes
Le présent document spécifie une méthode d'essai permettant de déterminer la durabilité naturelle d'un bois vis-à-vis des champignons basidiomycètes lignivores cultivés sur milieu agar. Ladite méthode est applicable à toutes les essences de bois.
NOTE Cette méthode peut être utilisée conjointement avec une épreuve de vieillissement, par exemple, selon l'EN 73 ou l'EN 84.
Trajnost lesa in lesnih proizvodov - Ugotavljanje naravne trajnosti masivnega lesa za zaščito proti lesnim gobam, preskusne metode - 1. del: Glive odprtotrosnice
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2006
7UDMQRVWOHVDLQOHVQLKSURL]YRGRY8JRWDYOMDQMHQDUDYQHWUDMQRVWLPDVLYQHJDOHVD
]D]DãþLWRSURWLOHVQLPJREDPSUHVNXVQHPHWRGHGHO *OLYHRGSUWRWURVQLFH
Durability of wood and wood-based products - Determination of the natural durability of
solid wood against wood-destroying fungi, test methods - Part 1: Basidiomycetes
Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung der natürlichen
Dauerhaftigkeit von Vollholz gegen holzzerstörende Pilze, Prüfverfahren - Teil 1:
Basidiomyceten
Durabilité du bois et des matériaux dérivés du bois - Détermination de la durabilité
naturelle du bois massif vis-a-vis des champignons lignivores, méthodes d'essai - Partie
1: Basidiomycetes
Ta slovenski standard je istoveten z: CEN/TS 15083-1:2005
ICS:
79.040 Les, hlodovina in žagan les Wood, sawlogs and sawn
timber
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
TECHNICAL SPECIFICATION
CEN/TS 15083-1
SPÉCIFICATION TECHNIQUE
TECHNISCHE SPEZIFIKATION
July 2005
ICS 79.040
English version
Durability of wood and wood-based products - Determination of
the natural durability of solid wood against wood-destroying
fungi, test methods - Part 1: Basidiomycetes
Durabilité du bois et des matériaux dérivés du bois - Dauerhaftigkeit von Holz und Holzprodukten - Bestimmung
Détermination de la durabilité naturelle du bois massif vis- der natürlichen Dauerhaftigkeit von Vollholz gegen
à-vis des champignons lignivores, méthodes d'essai - holzzerstörende Pilze, Prüfverfahren - Teil 1:
Partie 1: Basidiomycètes Basidiomyceten
This Technical Specification (CEN/TS) was approved by CEN on 1 March 2005 for provisional application.
The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.
CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 15083-1:2005: E
worldwide for CEN national Members.
Contents Page
Foreword .3
Introduction.4
1 Scope .5
2 Normative reference .5
3 Terms and definitions.5
4 Principle.5
5 Test materials and apparatus.5
6 Test specimens .8
7 Procedure .9
8 Test report .11
Annex A (informative) Guidance on sampling.13
Annex B (informative) Test fungi.14
Annex C (normative) Methods of sterilization .16
Annex D (informative) Assessment of results.17
Annex E (informative) Example of a test report .18
Bibliography.20
Foreword
This document (CEN/TS 15083-1:2005) has been prepared by Technical Committee CEN/TC 38 “Durability of
wood and wood-based products”, the secretariat of which is held by AFNOR.
This document consists of two parts. Part 1 is required to determine the natural durability of solid wood
against wood destroying basidiomycetes fungi and Part 2 against soft rotting micro-fungi.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this CEN Technical Specification: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and the United Kingdom.
Introduction
This CEN Technical Specification specifies a laboratory method of test which gives a basis for the assessment
of the natural durability of a sample of timber against attack by wood-destroying basidiomycetes. The natural
durability of a species of timber can vary depending on the conditions of growth such as climate and soil type.
For this reason, the durability established using the method described in this document will relate only to the
sample of timber tested. Guidance on sampling is given in Annex A.
This laboratory method provides one criterion by which the durability of the timber can be assessed. It is
recommended that this information be supplemented by data from other relevant tests, for example
CEN/TS 15083-2, and above all by practical experience.
The procedures described in this standard method are intended to be carried out by suitably trained and/or
supervised specialists.
1 Scope
This CEN Technical Specification specifies a method of test for determining the natural durability of a timber
against wood-destroying basidiomycetes cultured on an agar medium. The method is applicable to all timber
species.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73 or EN 84.
2 Normative reference
The following referenced document is indispensable for the application of this document. For dated references,
only the edition cited applies. For undated references, the latest edition of the referenced document (including
any amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
supplier
sponsor of the test (person or company providing the sample of timber to be tested)
4 Principle
Test specimens prepared from the timber under test and reference timber test specimens are exposed to
attack by pure cultures of wood-destroying basidiomycete fungi. After a prescribed period of incubation under
defined conditions, the percentage loss in dry mass of the test specimens is used to estimate the resistance of
the test timber to attack by the test fungi and as the basis of a provisional durability rating.
5 Test materials and apparatus
5.1 Biological material
5.1.1 Test fungi
The test fungi to be used are as follows.
5.1.1.1 Obligatory fungus in all cases: Coniophora puteana (Schumacher ex Fries) Karsten (BAM
Ebw. 15).
Loss in mass of Scots pine sapwood in 16 weeks: minimum 30 %.
Loss in mass of beech in 16 weeks: minimum 30 %.
5.1.1.2 Obligatory fungi for particular timbers:
Poria placenta (Fries) Cooke sensu J. Eriksson (FPRL 280) for soft woods.
Loss in mass of Scots pine sapwood in 16 weeks: minimum 20 %.
Coriolus versicolor (Linnaeus) Quélet (CTB 863A) for hardwoods.
Loss in mass of beech in 16 weeks: minimum 20 %.
5.1.1.3 Maintenance of strains
The strains shall be maintained and treated (frequency of subculturing, alternation of culture media, etc.) in
accordance with the instructions of their laboratory of origin (see B.2). The parent strain shall be maintained in
the laboratory of its origin so as to conserve and to assure its vigour.
NOTE If tests are not undertaken regularly or if a strain shows signs of degeneration a new standard culture of the
strain should be obtained from the laboratory of its origin for each test (see B.2).
When new strains are received, the virulence shall be tested to ensure the strain can achieve the minimum
loss in mass (see 5.1.1.1 and 5.1.1.2).
5.1.2 Reference timbers
5.1.2.1 Species used for the tests:
Scots pine sapwood (Pinus sylvestris Linnaeus) for tests with softwoods;
beech (Fagus sylvatica Linnaeus) for tests with hardwoods.
5.1.2.2 Wood quality
The wood shall be free from cracks, stain, decay, insect damage or other defects. The wood shall not have
been water-stored, floated, chemically treated or steamed.
NOTE Wood that has been kiln dried at temperatures below 60 °C can be used.
The Scots pine shall be exclusively sapwood containing little resin and having between 2,5 and 8 annual
growth rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole.
The beech shall be even-grained, free from tyloses and discolouration. It shall have between 2 and 6 annual
growth rings per 10 mm.
5.1.2.3 Provision of reference timber test specimens
Prepare planed strips having a cross-section of (25 ± 0,5) mm x (15 ± 0,5) mm. The longitudinal faces shall be
parallel to the direction of the grain. The annual rings shall not be parallel to the broad faces (contact angle to
be greater than 5) but otherwise may run in any direction. Make transverse cuts, neatly to give sharp edges
and a fine-sawn finish to the end-grain surfaces, to give reference timber test specimens (50 ± 0,5) mm long.
The test specimens shall originate from a minimum of three trees or shall be taken from a stock originally of
more than 500 test specimens and originating from at least five planks.
5.1.2.4 Dimensions and density of reference timber test specimens
The dimensions of each reference timber test specimen at a mass fraction of (12 ± 2) % moisture content
shall be (50 ± 0,5) x (25 ± 0,5) mm x (15 ± 0,5) mm.
NOTE A moisture meter of the two-pronged electrical conductivity type is suitable for assessing moisture content.
In a batch of test specimens, the density of an individual is permitted to differ from the mean value of the batch
by ± 10 %.
5.1.2.5 Number and distribution of reference timber test specimens
Use at least 10 reference timber test specimens for each test fungus. Mark each test specimen so that it can
be identified throughout the test.
5.2 Products and reagents
5.2.1 Culture medium
The culture medium shall be a malt agar medium with the following composition:
malt extract:
in concentrated form: (50 ± 0,5) g;
in powder form: (40 ± 0,5) g.
agar causing no inhibition of growth of fungi:
(20 ± 0,5) g to (30 ± 0,5) g.
water conforming to grade 3 of EN ISO 3696:
quantity to make up to 1 000 ml.
Prepare this medium by warming the mixture in a boiling water bath or steam bath, stirring until completely
dissolved.
Place in each culture vessel (5.3.1) a sufficient quantity of the medium to provide a minimum depth of 3 mm to
4 mm when in its in-use position. Close the vessels as specified in 5.3.1 and sterilize in an autoclave at
121 °C for 20 min. Let the vessels cool in their in-use position.
5.3 Apparatus
5.3.1 Culture vessels, Kolle flasks or equivalent vessels with a capacity of between 400 ml and 650 ml
providing a flat surface area of between 85 cm² and 120 cm² for the medium.
NOTE 1 Examples of suitable vessels are given in EN 113.
NOTE 2 Kolle flasks are usually plugged with a wad of cotton wool. Other culture vessels are usually fitted with
leakproof lids, the centres of which are pierced with a round hole of up to 15 mm diameter and plugged with a wad of
cotton wool.
5.3.2 Drying oven, capable of being controlled at (103 ± 2) °C.
5.3.3 Desiccators, with efficient desiccant (silica gel for example).
5.3.4 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and (65 ± 5) % relative humidity.
5.3.5 Culture chamber, (incubator or room) dark and controlled at (22 ± 2) °C and (70 ± 5) % relative
humidity.
5.3.6 Test specimens supports, made of glass, stainless steel or any other inert material, that is to say,
with no risk of having any effect on the culture medium, the fungus, the wood, or in itself being modified.
NOTE Supports may be capable of holding either one or two test specimens.
The supports are used to prevent direct contact of the test specimens with the culture medium, but shall not
separate them from it by more than 3 mm.
5.3.7 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g
and an autoclave.
6 Test specimens
6.1 Species and
...
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