ENV 14194:2002
(Main)Foodstuffs - Determination of saxitoxin and dc-saxitoxin in mussels - HPLC method using post column derivatisation
Foodstuffs - Determination of saxitoxin and dc-saxitoxin in mussels - HPLC method using post column derivatisation
This European Prestandard specifies a method for the quantitative determination of saxitoxin, dc-saxitoxin and the qualitative determination of neo-saxitoxin, and the gonyau toxins GTX-2 and GTX-3 in mussels. The method can also be used to identify the toxins C-1, C-2, GTX-5 and GTX-6 after hydrolysis and, if these toxins are present, to exclude false positive results for GTX-2, GTX-3, neo-saxitoxin and saxitoxin. For mussel the lowest limit of determination is for saxitoxin 0,04 mg/kg mussel meat and for dc-saxitoxin 0,03 mg/kg mussel meat (signal/noise = 10).
The upper limits of determination have not been determined. The limits of detection for C-1, C-2, GTX-2, GTX-3, GTX-5, GTX-6 and neo-saxitoxin have not been determined.
Lebensmittel - Bestimmung von Saxitoxin und DC-Saxitoxin in Muscheln - HPLC-Verfahren mit Nachsäulenderivatisierung
Diese Europäische Vornorm legt ein Verfahren zur quantitativen Bestimmung von Saxitoxin (Stx) und DC-Saxitoxin (DC-Stx) und zur qualitativen Bestimmung von Neo-Saxitoxin und den Gonyau-Toxinen GTX-2 und GTX-3 in Muscheln fest. Das Verfahren kann auch zum Nachweis der N-Sulfocarbamoyltoxine C-1, C-2, GTX-5 und GTX-6 nach Hydrolyse angewandt und bei Vorliegen dieser Toxine zum Ausschluss falsch positiver Ergebnisse für GTX-2, GTX-3, Neo-Saxitoxin und Saxitoxin verwendet werden. Bei Muscheln ist die Bestimmungsgrenze für Saxitoxin 0,04 mg/kg Muschelfleisch und für DC-Saxitoxin 0,03 mg/kg Muschelfleisch (Signal-Rausch-Verhältnis = 10).
Die Nachweisgrenzen für C-1, C-2, GTX-2, GTX-3, GTX-5, GTX-6 und Neo-Saxitoxin sind nicht ermittelt worden.
Produits alimentaires - Détermination de la teneur en saxitoxine et en dc-saxitoxine dans les moules - Méthode par chromatographie liquide haute performance après dérivation post-colonne
Cette Prénorme européenne spécifie une méthode de détermination quantitative de la saxitoxine (STX) et de la dc-saxitoxine (dc-STX) et de détermination qualitative de la néo-saxitoxine et des gonyautoxines GTX-2 et GTX-3 présentes dans les moules. Cette méthode peut également être utilisée pour identifier les toxines de N-sulfo-carbamoyles C-1, C-2, GTX-5 et GTX-6 après hydrolyse et, si ces toxines sont présentes, pour identifier les résultats faux positifs pour la GTX-2, la GTX-3, la néo-saxitoxine et la saxitoxine. Pour les moules, la limite de détection est de 0,04 mg/kg de chair de moule pour la saxitoxine, de 0,03 mg/kg de chair de moule pour la dc-saxitoxine (signal/bruit = 10).
Les limites de détection pour la C-1, la C-2, la GTX-2, la GTX-3, la GTX-5, la GTX-6 et la néo-saxitoxine n'ont pas été déterminées.
Živila - Določevanje saksitoksina in dc-saksitoksina v školjkah - Metoda HPLC z uporabo postkolonske derivatizacije
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
SIST ENV 14194:2002
01-september-2002
äLYLOD'RORþHYDQMHVDNVLWRNVLQDLQGFVDNVLWRNVLQDYãNROMNDK0HWRGD+3/&]
XSRUDERSRVWNRORQVNHGHULYDWL]DFLMH
Foodstuffs - Determination of saxitoxin and dc-saxitoxin in mussels - HPLC method using
post column derivatisation
Lebensmittel - Bestimmung von Saxitoxin und DC-Saxitoxin in Muscheln - HPLC-
Verfahren mit Nachsäulenderivatisierung
Produits alimentaires - Détermination de la teneur en saxitoxine et en dc-saxitoxine dans
les moules - Méthode par chromatographie liquide haute performance apres dérivation
post-colonne
Ta slovenski standard je istoveten z: ENV 14194:2002
ICS:
67.120.30 Ribe in ribji proizvodi Fish and fishery products
SIST ENV 14194:2002 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST ENV 14194:2002
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SIST ENV 14194:2002
EUROPEAN PRESTANDARD
ENV 14194
PRÉNORME EUROPÉENNE
EUROPÄISCHE VORNORM
April 2002
ICS 67.120.30
English version
Foodstuffs - Determination of saxitoxin and dc-saxitoxin in
mussels - HPLC method using post column derivatisation
Produits alimentaires - Détermination de la teneur en Lebensmittel - Bestimmung von Saxitoxin und DC-Saxitoxin
saxitoxine et en dc-saxitoxine dans les moules - Méthode in Muscheln - HPLC-Verfahren mit
par chromatographie liquide haute performance après Nachsäulenderivatisierung
dérivation post-colonne
This European Prestandard (ENV) was approved by CEN on 14 December 2001 as a prospective standard for provisional application.
The period of validity of this ENV is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the ENV can be converted into a European Standard.
CEN members are required to announce the existence of this ENV in the same way as for an EN and to make the ENV available promptly
at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the ENV) until the final
decision about the possible conversion of the ENV into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2002 CEN All rights of exploitation in any form and by any means reserved Ref. No. ENV 14194:2002 E
worldwide for CEN national Members.
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SIST ENV 14194:2002
ENV 14194:2002 (E)
Foreword
This document (ENV 14194:2002) has been prepared by Technical Committee CEN /TC 275, "Food analysis -
Horizontal methods", the secretariat of which is held by DIN.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this European Prestandard: Austria, Belgium, Czech Republic, Denmark, Finland,
France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Spain,
Sweden, Switzerland and the United Kingdom.
1 Scope
This European Prestandard specifies a method for the quantitative determination of saxitoxin (STX), dc-saxitoxin
(dc-STX) and the qualitative determination of neo-saxitoxin, and the gonyautoxins GTX-2 and GTX-3 in mussels.
The method can also be used to identify the N-sulfocarbamoyl toxins C-1, C-2, GTX-5 and GTX-6 after hydrolysis
and, if these toxins are present, to exclude false positive results for GTX-2, GTX-3, neo-saxitoxin and saxitoxin. For
mussel the limit of quantification is for saxitoxin 0,04 mg/kg mussel meat and for dc-saxitoxin 0,03 mg/kg mussel
meat (signal/noise = 10).
The limits of detection for C-1, C-2, GTX-2, GTX-3, GTX-5, GTX-6 and neo-saxitoxin have not been determined.
2 Normative references
This European Prestandard incorporates by dated or undated reference, provisions from other publications. These
normative references are cited at the appropriate places in the text and the publications are listed hereafter. For
dated references, subsequent amendments to or revisions of any of these publications apply to this European
Prestandard only when incorporated in it by amendment or revision. For undated references the latest edition of the
publication referred to applies (including amendments).
EN ISO 3696:1995, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
3 Principle
PSP (Paralytic Shellfish Poisoning) toxins are extracted from mussels homogenate with an acidic aqueous solution.
After centrifugation the supernatant is purified by solid phase extraction (SPE) over a
...
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