EN 16802:2016

Foodstuffs - Determination of elements and their chemical species - Determination of inorganic arsenic in foodstuffs of marine and plant origin by anion-exchange HPLC-ICP-MS

This draft European Standard describes a procedure for the determination of inorganic arsenic in foodstuffs of marine and plant origin by anion-exchange HPLC-ICP-MS following waterbath extraction.
This method has been validated in an interlaboratory test on white rice, wholemeal rice, leek, blue mussels, fish muscle and seaweed with an inorganic arsenic mass fraction in the range 0,073 mg/kg to 10,3 mg/kg [1].

Lebensmittel - Bestimmung von Elementen und ihren Verbindungen - Bestimmung von anorganischem Arsen in Lebensmitteln marinen Ursprungs und pflanzlichen Lebensmitteln mit Anionenaustausch-HPLC-ICP-MS

Diese Europäische Norm beschreibt ein Verfahren zur Bestimmung von anorganischem Arsen in marinen und pflanzlichen Lebensmitteln mit Anionenaustausch-HPLC-ICP-MS nach Wasserbadextraktion.
Dieses Verfahren wurde in einem Ringversuch an Reis (poliert), Vollkornreis, Porree (Lauch), Miesmuscheln, Fischfleisch und Meeresalgen mit einem Massenanteil an anorganischem Arsen von 0,073 mg/kg bis 10,3 mg/kg [1] validiert.

Produits alimentaires - Détermination des éléments et de leurs espèces chimiques - Détermination de la teneur en arsenic inorganique dans les produits alimentaires d'origines marine et végétale, par CLHP avec échange d'anions et spectrométrie de masse à plasma induit par haute fréquence (ICP-SM)

Le présent projet de Norme européenne spécifie un mode opératoire de détermination de la teneur en arsenic inorganique dans les produits alimentaires d’origines marine et végétale par HPLC à échange d'anions ICP MS après extraction par bain d'eau.
Cette méthode a été validée dans le cadre d’un essai interlaboratoires réalisé sur du riz blanc, du riz complet, du poireau, des moules bleues, du muscle de poisson et du varech dont la teneur massique en arsenic inorganique est comprise entre 0,073 mg/kg et 10,3 mg/kg.

Živila - Določevanje elementov in njihovih kemijskih oblik - Določevanje anorganskega arzena v živilih morskega in rastlinskega izvora z anionsko izmenjavo HPLC-ICP-MS

Ta osnutek evropskega standarda opisuje postopek za določevanje anorganskega arzena v živilih morskega in rastlinskega izvora z anionsko izmenjavo HPLC-ICP-MS po ekstrakciji v vodni kopeli.
Ta metoda je bila potrjena z medlaboratorijskim preskusom belega riža, polnozrnatega riža, pora, užitnih klapavic, mišičnine rib in morskih alg z masnim deležem anorganskega arzena v območju od 0,073 mg/kg do 10,3 mg/kg.

General Information

• Status: Published
• Publication Date: 29-Mar-2016
• Withdrawal Date: 29-Sep-2016
• ICS: 67.050 - General methods of tests and analysis for food products
• ICS: 67.060 - Cereals, pulses and derived products
• ICS: 67.120.30 - Fish and fishery products
• Technical Committee: CEN/TC 275 - Food analysis - Horizontal methods
• Drafting Committee: CEN/TC 275/WG 10 - Trace elements (heavy metals)
• Current Stage: 9093 - Decision to confirm - Review Enquiry
• Completion Date: 03-Sep-2021
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Mandate: M/422 - Mandate for standardisation addressed to CEN in the field of methods of analysis for heavy metals and iodine in food
• Ref Project: SIST EN 16802:2016 - Foodstuffs - Determination of elements and their chemical species - Determination of inorganic arsenic in foodstuffs of marine and plant origin by anion-exchange HPLC-ICP-MS

Standards Content (Sample)

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.þHYDQMHQLRQVNRLebensmittel - Bestimmung von Elementen und ihren Verbindungen - Bestimmung von anorganischem Arsen in Lebensmitteln marinen Ursprungs und pflanzlichen Lebensmitteln mit Anionenaustausch-HPLC-ICP-MSProduits alimentaires - Détermination des éléments et de leurs espèces chimiques - Détermination de la teneur en arsenic inorganique dans les produits alimentaires d'origines marine et végétale, par CLHP avec échange d'anions et spectrométrie de masse à plasma induit par haute fréquence (ICP-SM)Foodstuffs - Determination of elements and their chemical species - Determination of inorganic arsenic in foodstuffs of marine and plant origin by anion-exchange HPLC-ICP-MS67.120.30Ribe in ribji proizvodiFish and fishery products67.060QMLKCereals, pulses and derived products67.050Splošne preskusne in analizne metode za živilske proizvodeGeneral methods of tests and analysis for food productsICS:Ta slovenski standard je istoveten z:EN 16802:2016SIST EN 16802:2016en,fr,de01-junij-2016SIST EN 16802:2016SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16802
March
t r s x ICS
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x yä r x râ
x yä s t rä u r English Version
Foodstuffs æ Determination of elements and their chemical species æ Determination of inorganic arsenic in foodstuffs of marine and plant origin by anionæexchange HPLCæICPæMS Produits alimentaires æ Détermination des éléments et de leurs espèces chimiques æ Détermination de la teneur en arsenic inorganique dans les produits alimentaires d 5origines marine et végétaleá par CLHP avec échange d 5anions et spectrométrie de masse à
Lebensmittel æ Bestimmung von Elementen und ihren Verbindungen æ Bestimmung von anorganischem Arsen in Lebensmitteln marinen Ursprungs und pflanzlichen Lebensmitteln mit AnionenaustauschæHPLCæICPæMS This European Standard was approved by CEN on
z February
t r s xä
egulations which stipulate the conditions for giving this European Standard the status of a national standard without any alterationä Upætoædate lists and bibliographical references concerning such national standards may be obtained on application to the CENæCENELEC Management Centre or to any CEN memberä
translation under the responsibility of a CEN member into its own language and notified to the CENæCENELEC Management Centre has the same status as the official versionsä
CEN members are the national standards bodies of Austriaá Belgiumá Bulgariaá Croatiaá Cyprusá Czech Republicá Denmarká Estoniaá Finlandá Former Yugoslav Republic of Macedoniaá Franceá Germanyá Greeceá Hungaryá Icelandá Irelandá Italyá Latviaá Lithuaniaá Luxembourgá Maltaá Netherlandsá Norwayá Polandá Portugalá Romaniaá Slovakiaá Sloveniaá Spainá Swedená Switzerlandá Turkey and United Kingdomä
EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels
t r s x CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Membersä Refä Noä EN
s x z r tã t r s x ESIST EN 16802:2016

Precision data . 11 Annex B (informative)
Supplementary information about chromatographic conditions . 12 Bibliography . 13
°C) and acid of high purity, e.g. cleaned by sub-boiling distillation, are recommended. Reagents should be of minimum p.a. quality where possible. Special facilities can be used in order to avoid contamination during the steps of preparation and measurement (e.g. uses of laminar flow benches or comparable clean room facilities). 4.2 Nitric acid concentrated, mass fraction w(HNO3),
· 65 %, mass concentration of approximately (HNO3) = 1,4 g/ml. Use only nitric acid available with high purity or perform a clean-up by a sub-boiling distillation in order to avoid potential contamination. SIST EN 16802:2016

· 99,999 %, for production of mobile phase solution. 4.7 Aqueous ammonia, w[NH3(aq.)]
· 25 %, for adjustment of pH in the mobile phase. 4.8 Methanol, (CH3OH), HPLC grade, for production of mobile phase. 4.9 Mobile phase, e.g. 50 mmol/l ammonium carbonate in 3 % methanol at pH 10,3. Dissolve e.g. 4,80 g of ammonium carbonate (4.6) in approximately 800 ml of water. Adjust the pH to 10,3 with aqueous ammonia (4.7), add 30 ml of methanol (4.8) and fill up to 1 000 ml with water. Filter the mobile phase solution through a 0,45 µm filter prior to use. The optimal concentration of ammonium carbonate in the mobile phase depends on the analytical column used (e.g. brand, particle size and dimensions). The appropriate concentration of ammonia carbonate is at the discretion of the analyst and should fulfil the criteria for sufficient resolution of the arsenate peak as stated in 5.10. Methanol is added to the mobile phase in order to enhance the signal intensity for arsenic (carbon enhancement effect [2]). The concentration of methanol for maximum signal depends on the instrument used and should be identified by the analyst. 4.10 Diarsenic trioxide, w(As2O3)
· 99,5 %, optional. 4.11 Potassium hydroxide solution, (KOH) = 20 g/100 ml, optional. 4.12 Sulfuric acid solutions, w(H2SO4) = 20 % and w(H2SO4) = 1 %, optional. 4.13 Phenolphthalein, optional. 4.14 Standard solutions, with an arsenic mass concentration of 1 000 mg/l. The use of commercial standards of arsenic, arsenic III and/or V, with a mass concentration of 1 000 mg/l is recommended. SIST EN 16802:2016
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