EN ISO 20150:2019

Footwear and footwear components - Quantitative challenge test method to assess antifungal activity (ISO 20150:2019)

This document specifies quantitative challenge test methods for evaluating the antifungal activity of footwear and footwear components.
This document is applicable only to footwear and components that claim to have antifungal (antimycotic) properties or antimicrobial properties.
Two methods can be applied. The choice of method depends on the material properties and test microorganisms. Dynamic challenge test method can be applied to all types of materials. For single absorbent materials, static challenge test method is recommended. Brief descriptions of each method are given in 11.2 and 11.3.

Schuhe und Schuhbestandteile - Quantitatives Challengetestverfahren zur Bestimmung der antimykotischen Wirksamkeit (ISO 20150:2019)

Die Internationale Norm legt quantitative Challenge Testverfahren zur Bewertung der antifungalen Wirkung von Schuhen und Schuhbestandteilen fest.
Diese Internationale Norm ist ausschließlich auf Schuhe und Schuhbestandteile anwendbar, die antifungale (antimykotische) oder antimikrobielle Eigenschaften für sich beanspruchen.
WARNUNG — Diese Norm berücksichtigt keine Aspekte des Arbeits  und Gesundheitsschutzes in Bezug auf bei der Behandlung eingesetzte Antimykotika. Anwender dieser Norm sollten allgemeine Anforderungen an die Biosicherheit erfüllen und geeignete Gesundheits  und Sicherheitsvorschriften festlegen.

Chaussures et composants de chaussure - Méthode de test d'épreuve quantitatif pour évaluer l'activité antifongique (ISO 20150:2019)

Le présent document spécifie des méthodes de test d'épreuve quantitatif permettant d'évaluer l'activité antifongique des chaussures et de leurs composants.
Le présent document n'est applicable qu'aux chaussures et composants de chaussures pour lesquels des propriétés antifongiques (antimycotiques) ou antimicrobiennes sont revendiquées.
Deux méthodes peuvent être appliquées. Le choix de la méthode dépend des propriétés du matériau et des micro-organismes d'essai. La méthode de test d'épreuve dynamique peut être appliquée à tous types de matériaux. Pour les matériaux absorbants non combinés, il est recommandé d'appliquer la méthode de test d'épreuve statique. Des descriptions succinctes de ces méthodes figurent en 11.2 et 11.3.

Obutev in sestavni deli obutve - Kvantitativna preskusna metoda za ocenjevanje protiglivičnega delovanja (ISO 20150:2019)

Ta standard določa preskusno metodo (preskus rasti) za kvantitativno ovrednotenje protiglivičnega delovanja obutve in sestavnih delov obutve zaradi delovanja mikrogliv. Ta mednarodni standard se uporablja samo za obutev in sestavne dele obutve, za katere se trdi, da imajo protiglivične (antimikotične) ali antimikrobne terapevtske učinke.

General Information

• Status: Published
• Publication Date: 19-Feb-2019
• Withdrawal Date: 30-Aug-2019
• ICS: 61.060 - Footwear
• Technical Committee: CEN/TC 309 - Footwear
• Drafting Committee: CEN/TC 309 - Footwear
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 20-Feb-2019
• Due Date: 17-Aug-2019
• Completion Date: 20-Feb-2019
• Ref Project: SIST EN ISO 20150:2019 - Footwear and footwear components - Quantitative challenge test method to assess antifungal activity (ISO 20150:2019)

Standards Content (Sample)

SLOVENSKI STANDARD
01-maj-2019
2EXWHYLQVHVWDYQLGHOLREXWYH.YDQWLWDWLYQDSUHVNXVQDPHWRGD]DRFHQMHYDQMH
SURWLJOLYLþQHJDGHORYDQMD,62
Footwear and footwear components - Quantitative challenge test method to assess
antifungal activity (ISO 20150:2019)
Schuhe und Schuhbestandteile - Qualitative Prüfverfahren zur Bestimmung der Aktivität
gegen Pilzbefall (ISO 20150:2019)
Chaussures et composants de chaussure - Méthode de test d'épreuve quantitatif pour
évaluer l'activité antifongique (ISO 20150:2019)
Ta slovenski standard je istoveten z: EN ISO 20150:2019
ICS:
61.060 Obuvala Footwear
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 20150
EUROPEAN STANDARD
NORME EUROPÉENNE
February 2019
EUROPÄISCHE NORM
ICS 61.060
English Version
Footwear and footwear components - Quantitative
challenge test method to assess antifungal activity (ISO
20150:2019)
Chaussures et composants de chaussure - Méthode de Schuhe und Schuhbestandteile - Quantitatives
test d'épreuve quantitatif pour évaluer l'activité Challengetestverfahren zur Bestimmung der
antifongique (ISO 20150:2019) antimykotischen Wirksamkeit (ISO 20150:2019)
This European Standard was approved by CEN on 28 December 2018.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20150:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 20150:2019) has been prepared by Technical Committee ISO/TC 216
"Footwear" in collaboration with Technical Committee CEN/TC 309 “Footwear” the secretariat of which
is held by UNE.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by August 2019, and conflicting national standards shall
be withdrawn at the latest by August 2019.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 20150:2019 has been approved by CEN as EN ISO 20150:2019 without any modification.

INTERNATIONAL ISO
STANDARD 20150
First edition
2019-01
Footwear and footwear components —
Quantitative challenge test method to
assess antifungal activity
Chaussures et composants de chaussure — Méthode de test d'épreuve
quantitatif pour évaluer l'activité antifongique
Reference number
ISO 20150:2019(E)
©
ISO 2019
ISO 20150:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: [phone]
Fax: [phone]
Email: [e-mail]
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 20150:2019(E)
Contents Page
Foreword .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Safety . 2
6 Apparatus . 2
7 Reagents and culture medium . 3
7.1 General . 3
7.2 Water . 3
7.3 Malt medium . 3
7.3.1 Composition . 3
7.3.2 Preparation . 3
7.4 Malt extract agar (MEA) medium . 4
7.4.1 Composition . 4
7.4.2 Preparation . 4
7.5 Physiological saline (sodium chloride solution) . 4
7.5.1 Composition . 4
7.5.2 Preparation . 4
7.6 Wetting agent (nonionic surfactant) . 4
7.7 Buffer solution . 4
7.7.1 Buffer stock . 4
7.7.2 Preparation of buffer stock . 5
7.7.3 Preparation of buffer solution . 5
8 Test microorganisms . 5
9 Preparation of test inoculums . 5
9.1 Indications for use of strains . 5
9.2 Preparation of inoculums of Candida albicans . 6
9.3 Preparation of test spore suspension of filamentous micro-fungi . 6
10 Preparation of test specimens . 6
10.1 General . 6
10.2 Test specimen . 7
10.3 Pre-treatment of test specimen . 7
11 Test procedure . 7
11.1 Summary of test methods . 7
11.2 Dynamic challenge tests . 7
11.2.1 Inoculation . 7
11.2.2 Neutralization and elution after inoculation (time 0 h) . 8
11.2.3 Incubation . 8
11.2.4 Neutralization and elution after incubation (time 24 h) . 8
11.2.5 Determination of the number of viable micro-fungi . 8
11.3 Static challenge test . 8
11.3.1 Inoculation . 8
11.3.2 Neutralization and elution after inoculation (time 0 h) . 9
11.3.3 Incubation . 9
11.3.4 Neutralization and elution after incubation (time 24 h) . 9
11.3.5 Determination of the number of viable micro-fungi . 9
12 Expression of results
...