EN ISO 9167-1:1995

Rapeseed - Determination of glucosinolates content - Part 1: Method using high-performance liquid chromatography (ISO 9167-1:1992)

The method specified is based on extraction by methanol, purification and enzymatic desulfatation, determination using reversed-phase chromatography. Does not determine glucosinolates which are substituted on the glucose molecule. A rapid method for the determination of glucosinolates content using X-ray fluorescence spectrometry is the subject of ISO 9167-2.

Rapssamen - Bestimmung des Glucosinolatgehaltes - Teil 1: HPLC-Verfahren (ISO 9167-1:1992)

In diesem Teil von ISO 9167 wird ein Verfahren zur Bestimmung der verschiedenen Glucosinolatgehalte in Rapssamen (Kolza) mittels Hochleistungsflüssigkeitschromatographie festgelegt. Anmerkung 1: Glucosinolate, die am Glucose-Molekül substituiert sind, werden mit diesem Verfahren nicht erfaßt, sind für handelsübliche Rapssamen jedoch auch von untergeordneter Bedeutung. Anmerkung 2: Ein Schnellverfahren zur Bestimmung der Anteile der Glucosinolate unter Anwendung der Röntgenfluoreszensspektroskopie ist Thema von ISO 9267-2.

Graines de colza - Dosage des glucosinolates - Partie 1: Méthode par chromatographie liquide à haute performance (ISO 9167-1:1992)

La présente partie de l'ISO 9167 prescrit une méthode de dosage par chromatographie liquide à haute performance (CLHP) des différents glucosinolates dans les graines de colza.
NOTES 1 Cette méthode ne dose pas les glucosinolates ayant des substituants sur la partie glucose, mais ces composés sont peu importants dans les graines de colza commercialisées. 2 Une méthode rapide de dosage global des glucosinolates des graines de colza, par spectrométrie de fluorescence aux rayons X (XRF) fait l'objet de l'ISO 9167-2.

Seme oljne repice - Določevanje glukozinolatov - 1. del: Metoda s tekočinsko kromatografijo visoke ločljivosti (ISO 9167-1:1992)

Ta del ISO 9167 določa metodo za določevanje različnih glukozinolatov v oljni repici (repno seme) s tekočinsko kromatografijo visoke ločljivosti. OPOMBA 1: Ta metoda ne določa glukozinolatov, ki so v molekuli glukoze nadomeščeni, vendar te spojine v okviru komercialne oljne repice nimajo velikega pomena. OPOMBA 2: ISO 9167-2 opisuje hitro metodo določevanja glukozinolatov z rentgensko fluorescenčno spektrometrijo.

General Information

• Status: Withdrawn
• Publication Date: 22-Aug-1995
• Withdrawal Date: 18-Jun-2019
• ICS: 67.200.20 - Oilseeds
• Technical Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Drafting Committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Completion Date: 19-Jun-2019
• Ref Project: SIST EN ISO 9167-1:1998 - Rapeseed - Determination of glucosinolates content - Part 1: Method using high-performance liquid chromatography (ISO 9167-1:1992)

Relations

• Replaced By: EN ISO 9167:2019 - Rapeseed and rapeseed meals - Determination of glucosinolates content - Method using high-performance liquid chromatography (ISO 9167:2019)
• Effective Date: 26-Jun-2019
• Amended By: EN ISO 9167-1:1995/A1:2013 - Rapeseed - Determination of glucosinolates content - Part 1: Method using high-performance liquid chromatography - Amendment 1 (ISO 9167-1:1992/Amd 1:2013)
• Effective Date: 08-Jun-2022

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 9167-1:1998
01-november-1998
6HPHROMQHUHSLFH'RORþHYDQMHJOXNR]LQRODWRYGHO0HWRGDVWHNRþLQVNR
NURPDWRJUDILMRYLVRNHORþOMLYRVWL,62
Rapeseed - Determination of glucosinolates content - Part 1: Method using high-
performance liquid chromatography (ISO 9167-1:1992)
Rapssamen - Bestimmung des Glucosinolatgehaltes - Teil 1: HPLC-Verfahren (ISO 9167
-1:1992)
Graines de colza - Dosage des glucosinolates - Partie 1: Méthode par chromatographie
liquide a haute performance (ISO 9167-1:1992)
Ta slovenski standard je istoveten z: EN ISO 9167-1:1995
ICS:
67.200.20 Oljnice Oilseeds
SIST EN ISO 9167-1:1998 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN ISO 9167-1:1998

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SIST EN ISO 9167-1:1998

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SIST EN ISO 9167-1:1998

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SIST EN ISO 9167-1:1998

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SIST EN ISO 9167-1:1998

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SIST EN ISO 9167-1:1998
INTERNATIONAL
91674
STANDARD
First edition
1992-07-0 1
- ----
Rapeseed - Determination of glucosinolates
content -
Part 1:
Method using high-performance liquid
chromatography
Graines de colza - Dosage des qlucosinolates -
L
Partie 1: Methode par chromatographie liquide 3 haute perforrnance
~---- ---
--
Reference number
ISO 9167-1:1992(E)

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SIST EN ISO 9167-1:1998
ISO 91674:1992(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation of national Standards bodies (ISO member bodies). The work
of preparing International Standards is normally carried out through ISO
technical committees. Esch member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, govern-
mental and non-governmental, in liaison with ISO, also take part in the
work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an Inter-
national Standard requires approval by at least 75 % of the member
bodies casting a vote.
International Standard ISO 9167-1 was prepared by Technical Committee
ISO/TC 34, Agricultural food products, Sub-Committee SC 2, Oleaginous
seeds and fruits.
ISO 9167 consists of the following Parts, under the general title
Rapeseed - Determination of glucosinolates content:
-
Part 1: Method using high-performance liquid chromatography
-
Part 2: Method using X-ray fluorescence spectrometry
Annex A of this part of ISO 9167 is for information only.
0 ISO 1992
All rights reserved. No part of this publication may be reproduced or utilized in any form
or by any means, electronie or mechanical, including photocopylng and microfilm, without
Permission in writing from the publisher.
Intern ational Organizati on for Standardiz ation
211 Gen&e 20 * Switz erland
Case Postale 56 l CH-l
Printed in Switzerland
ii

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SIST EN ISO 9167-1:1998
ISO 91674:1992(E)
INTERNATIONAL STANDARD
- Determination of glucosinolates content -
Rapeseed
Part 1:
Method using high-performance liquid chromatography
1 Scope 3 Principle
Extraction of glucosinolates by methanol, then puri-
This part of ISO 9167 specifies a method for the
fication and enzymatic desulfatation on ion-
determination of the content of the different gluco-
exchange resins. Determination using
sinolates in rapeseeds (colza) using high-
reversed-Phase
high-performance liquid chro-
Performance liquid chromatography.
matography (HPLC) with elution gradient and ultra-
NOTES
violet detection.
1 This method does not determine gfucosinolates which
are substituted on the glucose molecule, but these com-
4 Reagents
pounds are of little importante in commercial rapeseed.
2 A rapid method for the determination of giucosinolates
Use only reagents of recognized analytical grade,
content using X-ray fluorescence spectrometry is the
unless otherwise specified, and water complying
subject of ISO 9167-2.
with grade 2 of ISO 3696.
4.1 Methanol, HPLC grade, 70 % (I/!I/) Solution.
4.2 Sodium acetate, 0,02 mol/1 at pH 4,0.
2 Normative references
4.3 Sodium acetate, 0,2 mol/1 solution.
The following Standards contain provisions which,
through reference in this text, constitute provisions
of this part of ISO 9167. At the time of publication,
4.4 Imidazofe formate, 6 mol/1 Solution.
the editions indicated were valid. Al
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