EN ISO 19250:2013

Water quality - Detection of Salmonella spp. (ISO 19250:2010)

ISO 19250:2010 specifies a method for the detection of Salmonella spp. (presumptive or confirmed) in water samples. It is possible that, for epidemiological purposes or during outbreak investigations, other media are also required.

Wasserbeschaffenheit - Bestimmung von Salmonella spp. (ISO 19250:2010)

Diese Internationale Norm legt ein Verfahren zum Nachweis von Salmonella spp. (präsumtiv oder bestätigt) in Wasserproben fest. Es ist möglich, dass bei besonderen epidemiologischen Situationen oder bei Untersuchungen von Krankheitsausbrüchen auch andere Medien benötigt werden.
WARNUNG — Es ist möglich, dass das Verfahren nicht alle Salmonella ser. Typhi und ser. Paratyphi erfasst.
ANMERKUNG Für eine semi-quanitative Untersuchung können MPN-Ansätze (en: most probable number – wahrscheinlichste Zahl) unter Verwendung geeigneter Probenvolumen durchgeführt werden. Für diese Fälle wird das Volumen des gepufferten Peptonwassers entsprechend angepasst.

Qualité de l'eau - Recherche de Salmonella spp. (ISO 19250:2010)

L'ISO 19250:2010 spécifie une méthode pour la recherche de Salmonella spp. (présomptives ou confirmées) dans des échantillons d'eau. À des fins épidémiologiques ou au cours d'enquêtes sur des épidémies, il peut être nécessaire d'utiliser d'autres milieux.

Kakovost vode - Ugotavljanje prisotnosti Salmonella spp. (ISO 19250:2010)

Ta mednarodni standard določa metodo za določanje prisotnosti Salmonella spp. (domnevne ali potrjene) v vzorcih vode. Za epidemološke namene ali med raziskovanjem izbruha so lahko zahtevani tudi drugi mediji.

General Information

• Status: Published
• Publication Date: 09-Apr-2013
• Withdrawal Date: 30-Oct-2013
• ICS: 07.100.20 - Microbiology of water
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230 - Water analysis
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 10-Apr-2013
• Due Date: 16-Jun-2013
• Completion Date: 10-Apr-2013
• Ref Project: SIST EN ISO 19250:2013 - Water quality - Detection of Salmonella spp. (ISO 19250:2010)

Standards Content (Sample)

SLOVENSKI STANDARD
01-julij-2013
1DGRPHãþD
SIST ISO 6340:1998
Kakovost vode - Ugotavljanje prisotnosti Salmonella spp. (ISO 19250:2010)
Water quality - Detection of Salmonella spp. (ISO 19250:2010)
Wasserbeschaffenheit - Bestimmung von Salmonella spp. (ISO 19250:2010)
Qualité de l'eau - Recherche de Salmonella spp. (ISO 19250:2010)
Ta slovenski standard je istoveten z: EN ISO 19250:2013
ICS:
07.100.20 Mikrobiologija vode Microbiology of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 19250
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2013
ICS 07.100.20
English Version
Water quality - Detection of Salmonella spp. (ISO 19250:2010)
Qualité de l'eau - Recherche de Salmonella spp. (ISO Wasserbeschaffenheit - Bestimmung von Salmonella spp.
19250:2010) (ISO 19250:2010)
This European Standard was approved by CEN on 21 March 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUR OP ÄIS CHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 19250:2013: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
The text of ISO 19250:2010 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 19250:2013 by
Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by October 2013, and conflicting national standards shall be withdrawn at
the latest by October 2013.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 19250:2010 has been approved by CEN as EN ISO 19250:2013 without any modification.

INTERNATIONAL ISO
STANDARD 19250
First edition
2010-07-15
Water quality — Detection of Salmonella
spp.
Qualité de l'eau — Recherche de Salmonella spp.

Reference number
ISO 19250:2010(E)
©
ISO 2010
ISO 19250:2010(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.

©  ISO 2010
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail [e-mail]
Web www.iso.org
Published in Switzerland
ii © ISO 2010 – All rights reserved

ISO 19250:2010(E)
Contents Page
Foreword .iv
Introduction.v
1 Scope.1
2 Normative references.1
3 Terms and definitions .2
4 Principle.2
4.1 General .2
4.2 Pre-enrichment in non-selective liquid medium .2
4.3 Enrichment in selective liquid media .2
4.4 Plating out and recognition.3
4.5 Confirmation .3
5 Apparatus.3
6 Sampling.4
7 Culture media and reagents .4
8 Procedure.5
8.1 Preparation of the sample .5
8.2 Non-selective pre-enrichment.5
8.3 Selective enrichment.5
8.4 Plating out .6
8.5 Confirmation .6
9 Expression of results.9
10 Test report.9
Annex A (normative) Diagram of procedure .10
Annex B (normative) Composition and preparation of culture media and reagents.11
Annex C (informative) Results of the interlaboratory trial.18
Bibliography.23

ISO 19250:2010(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 19250 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 4,
Microbiological methods.
This edition cancels and replaces ISO 6340:1995, which has been technically revised.

iv © ISO 2010 – All rights reserved

ISO 19250:2010(E)
Introduction
Salmonella species are bacteria which are widely distributed all over the world. They are usually classified as
pathogens, although their virulence and pathogenesis vary widely. The natural hosts of Salmonella include
humans, agricultural and domestic livestock, and wild animals including birds. Humans and animals can
excrete these bacteria while carrying them asymptomatically as well as during disease. It is therefore
impossible to eliminate them from the environment. Following the infection of humans, the transmission of
Salmonella can cause severe disease.
Since water is a recognized vehicle of infection, the presence or absence of Salmonella is monitored in water
where there is perceived to be a risk of infection. Salmonella can be present in all types of domestic and
agricultural waste water, freshwaters, including ground and drinking waters, as well as sea water.
The detection of Salmonella in water usually requires a concentration step. Since Salmonella cells can be
present in low numbers and injured in the aqueous environment, their detection in water usually requires a
pre-enrichment step.
INTERNATIONAL STANDARD ISO 19250:2010(E)

Water quality — Detection of Salmonella spp.
WARNING — In order to safeguard the health of laboratory personnel, it is essential that tests for
detecting Salmonella, and especially S. enterica subsp. enterica ser. Typhi (Salmonella ser. Typhi) and
S. enterica subsp. enterica ser. Paratyphi (Salmonella ser. Paratyphi), be undertaken only in properly
equipped laboratories, under the control of a skilled microbiologist, and that great care be taken in the
disposal of all incubated materials.
Persons using this International Standard should be familiar with normal laboratory practice. This
standard does not purport to address all of the safety problems, if any, associated with its use. It is
the responsibility of the user to establish appropriate safety and health practices and to ensure
compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this International Standard
be carried out by suitably trained staff.
1 Scope
This International Standard specifies a method for the detection of Salmonella spp. (presumptive or
confirmed) in water samples. It is possible that, for epidemiological purposes or during outbreak investigations,
other media are also required.
WARNING — It is possible that the method does not recover all Salmonella ser. Typhi and ser.
Paratyphi.
NOTE For a semi-quantitative approach, most
...