EN ISO 15753:2006
(Main)Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons (ISO 15753:2006)
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons (ISO 15753:2006)
ISO 15753:2006 describes two methods for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in animal and vegetable fats and oils: a general method, and a method specific for coconut oil and short-chain vegetable oils.
These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed using this method.
Tierische und pflanzliche Fette und Öle - Bestimmung von polycyclischen aromatischen Kohlenwasserstoffen (ISO 15753:2006)
Diese Internationale Norm beschreibt zwei Verfahren zur Bestimmung von 15 polycyclischen aromatischen Kohlenwasserstoffen (PAKs) in tierischen und pflanzlichen Fetten und Ölen:
- ein allgemeines Verfahren und
- ein Verfahren, speziell für Kokosfett und Pflanzenöle mit kurzkettigen Fettsäuren.
Diese Verfahren sind bei leicht flüchtigen Verbindungen, wie z. B. Naphthalin, Acenaphthen und Fluoren nicht quantitativ. Aufgrund von Störungen, die durch die Matrix selbst bedingt sind, können Palmöl und Oliven¬tresteröl nicht mit diesem Verfahren analysiert werden.
Die Bestimmungsgrenze beträgt bei fast allen analysierten Verbindungen 0,2 g/kg, mit Ausnahme von Fluoranthen und Benzo(g,h,i)perylen, deren Bestimmungsgrenze bei 0,3 g/kg liegt, sowie Indeno(1,2,3 c,d)pyren mit einer Bestimmungsgrenze von 1 g/kg.
Corps gras d'origines animale et végétale - Détermination des hydrocarbures aromatiques polycycliques (ISO 15753:2006)
L'ISO 15753:2006 décrit deux méthodes pour la détermination de 15 hydrocarbures aromatiques polycycliques (HAP) dans les corps gras d'origines animale et végétale: une méthode générale et une méthode spécifique pour l'huile de coprah et les huiles végétales avec acides gras à chaînes courtes.
Ces méthodes ne sont pas quantitatives pour les composés très volatils tels que le naphtalène, l'acénaphtène et le fluorène. En raison des interférences causées par la matrice elle-même, l'huile de palme et l'huile de grignons d'olive ne peuvent pas être analysées par cette méthode.
Rastlinske in živalske maščobe in olja - Določevanje policikličnih aromatskih ogljikovodikov (ISO 15753:2006)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-marec-2007
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHSROLFLNOLþQLKDURPDWVNLK
RJOMLNRYRGLNRY ,62
Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons
(ISO 15753:2006)
Tierische und pflanzliche Fette und Öle - Bestimmung von polycyclischen aromatischen
Kohlenwasserstoffen (ISO 15753:2006)
Corps gras d'origines animale et végétale - Détermination des hydrocarbures
aromatiques polycycliques (ISO 15753:2006)
Ta slovenski standard je istoveten z: EN ISO 15753:2006
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 15753
NORME EUROPÉENNE
EUROPÄISCHE NORM
September 2006
ICS 67.200.10
English Version
Animal and vegetable fats and oils - Determination of polycyclic
aromatic hydrocarbons (ISO 15753:2006)
Corps gras d'origines animale et végétale - Détermination Tierische und pflanzliche Fette und Öle - Bestimmung von
des hydrocarbures aromatiques polycycliques (ISO polycyclischen aromatischen Kohlenwasserstoffen (ISO
15753:2006) 15753:2006)
This European Standard was approved by CEN on 19 August 2006.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
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EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 15753:2006: E
worldwide for CEN national Members.
Foreword
This document (EN ISO 15753:2006) has been prepared by Technical Committee ISO/TC 34
"Agricultural food products" in collaboration with Technical Committee CEN/TC 307 "Oilseeds,
vegetable and animal fats and oils and their by-products - Methods of sampling and analysis",
the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by March 2007, and conflicting national
standards shall be withdrawn at the latest by March 2007.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
Endorsement notice
The text of ISO 15753:2006 has been approved by CEN as EN ISO 15753:2006 without any
modifications.
INTERNATIONAL ISO
STANDARD 15753
First edition
2006-09-01
Animal and vegetable fats and oils —
Determination of polycyclic aromatic
hydrocarbons
Corps gras d'origines animale et végétale — Détermination des
hydrocarbures aromatiques polycycliques
Reference number
ISO 15753:2006(E)
©
ISO 2006
ISO 15753:2006(E)
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ii © ISO 2006 – All rights reserved
ISO 15753:2006(E)
Contents Page
Foreword. iv
1 Scope. 1
2 Normative references. 1
3 Terms and definitions. 1
4 Principle. 2
5 Reagents and materials . 2
6 Apparatus. 3
7 Sampling. 4
8 Sample preparation. 5
9 Procedure for determination of PAHs from fats and oils: General method . 5
9.1 Preliminary remarks. 5
9.2 Blank. 5
9.3 Determination of recovery values (without matrix) . 5
9.4 Extraction (liquid/liquid extraction) . 6
9.5 Purification on C18-bonded phase cartridge (solid/liquid extraction). 6
9.6 Purification on Florisil-bonded phase cartridge (solid/liquid extraction). 6
10 Procedure for determination of PAHs from fats and oils: Method specific for coconut oil . 7
10.1 First extraction (liquid/liquid extraction). 7
10.2 Second extraction (liquid/liquid extraction). 8
10.3 Purification on C18-bonded phase cartridge (solid/liquid extraction). 8
10.4 Purification on Florisil-bonded phase cartridge (solid/liquid extraction). 8
11 High-performance liquid chromatography (HPLC) . 9
11.1 Operating conditions. 9
11.2 Detection parameters . 10
11.3 Analysis of samples and standards. 11
11.4 Confirmation of the presence of PAHs. 12
12 Expression of results. 12
13 Precision. 12
13.1 Interlaboratory test . 12
13.2 Repeatability. 13
13.3 Reproducibility. 13
14 Test report. 13
Annex A (informative) Recovery values, flow charts, chromatograms and injection sequences . 14
Annex B (informative) Results of the interlaboratory test . 19
Bibliography . 22
ISO 15753:2006(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 15753 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal
and vegetable fats and oils.
iv © ISO 2006 – All rights reserved
INTERNATIONAL STANDARD ISO 15753:2006(E)
Animal and vegetable fats and oils — Determination of
polycyclic aromatic hydrocarbons
1 Scope
This International Standard describes two methods for the determination of 15 polycyclic aromatic
hydrocarbons (PAHs) in animal and vegetable fats and oils:
⎯ a general method, and
⎯ a method specific for coconut oil and vegetable oils with short-chain fatty acids.
These methods are not quantitative for the very volatile compounds such as naphthalene, acenaphthene and
fluorene. Due to interferences provided by the matrix itself, palm oil and olive pomace oil cannot be analysed
using this method.
The quantification limit is 0,2 µg/kg for almost all compounds analysed, except for fluoranthene and
benzo(g,h,i)perylene where the quantification limit is 0,3 µg/kg, and indeno(1,2,3-c,d)pyrene where the
quantification limit is 1 µg/kg.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 661, Animal and vegetable fats and oils — Preparation of test sample
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
polycyclic aromatic hydrocarbon
PAH
compound that contains two or more condensed (fused) aromatic hydrocarbon rings and the content of which
can be determined according to the method specified in this International Standard
NOTE 1 The content is given in micrograms per kilogram.
NOTE 2 In general PAHs are divided into light PAHs with two to four aromatic rings, and heavy PAHs with five or more
aromatic rings.
EXAMPLES
Light PAHs include:
naphthalene (CAS RN [91-20-3]), acenaphthene (CAS RN [83-32-9]), acenaphthylene (CAS RN [208-96-8]), fluorene
(CAS RN [86-73-7]), anthracene (CAS RN [120-12-7]), phenanthrene (CAS RN [85-01-8]), fluoranthene (CAS RN [206-44-0]),
chrysene (CAS RN [218-01-9]), benz(a)anthracene (CAS RN [56-55-3]), pyrene (CAS RN [129-00-0]).
ISO 15753:2006(E)
Heavy PAHs include:
benzo(a)pyrene (CAS RN [50-32-8]), benzo(b)fluoranthene (CAS RN [205-99-2]), benzo(k)fluoranthene
(CAS RN [207-08-9]), benzo(g,h,i)per
...
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