Foodstuffs - Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence detection after precolumn derivatization

This Technical Specification specifies a method for the determination of fumonisin B1 (FB1) and fumonisin B2 (FB2) in processed maize-containing foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection (FLD). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 112 µg/kg to 458 µg/kg for FB1+FB2, 89 µg/kg to 384 µg/kg for FB1 and 22 µg/kg to 74 µg/kg for FB2.
For further information on the validation see Clause 8 and Annex B.

Lebensmittel - Bestimmung von Fumonisin B1 und Fumonisin B2 in Säuglings- und Kleinkindernahrung auf Maisbasis - HPLC-Verfahren mit Reinigung an einer Immunoaffinitätssäule und Fluoreszenzdetektion nach Vorsäulenderivatisierung

Diese Technische Spezifikation legt ein Verfahren zur Bestimmung von Fumonisin B1 (FB1) und Fumonisin B2
(FB2) in verarbeiteten Mais enthaltenden Lebensmitteln für Säuglinge und Kleinkinder durch Hochleistungsflüssigchromatographie
(HPLC) mit Reinigung an einer Immunoaffinitätssäule und Fluoreszenzdetektion
(FLD) fest. Dieses Verfahren wurde in einem Ringversuch durch die Untersuchung sowohl von natürlich
kontaminierten Proben als auch von aufgestockten Proben mit Gehalten von 112 µg/kg bis 458 µg/kg für
FB1 + FB2, 89 µg/kg bis 384 µg/kg für FB1 und 22 µg/kg bis 74 µg/kg für FB2 validiert.
Weitere Informationen zur Validierung befinden sich in Abschnitt 8 und Anhang B.

Denrées alimentaires - Dosage de la fumonisine B1 et de la fumonisine B2 dans les aliments pour nourrissons et jeunes enfants contenant du maïs transformé - Méthode par CLHP avec purification sur colonne d'immunoaffinité et détection de fluorescence après dérivation précolonne

La présente Spécification technique spécifie une méthode de dosage de la fumonisine B1 (FB1) et de la
fumonisine B2 (FB2) dans les aliments pour nourrissons et jeunes enfants contenant du maïs transformé par
chromatographie liquide haute performance (CLHP) avec purification sur colonne d’immunoaffinité et
détection par fluorescence (DFL). Cette méthode a été validée lors d’une étude interlaboratoires en procédant
à l’analyse d’échantillons naturellement contaminés et supplémentés à des teneurs comprises entre
112 µg/kg et 458 µg/kg en FB1+FB2, entre 89 µg/kg et 384 µg/kg en FB1 et entre 22 µg/kg et 74 µg/kg en FB2.
Pour de plus amples informations sur la validation, voir l’Article 8 et l’Annexe B.

Živila - Določevanje fumonizina B1 in fumonizina B2 v predelanih hrani na osnovi koruze za dojenčke in majhne otroke - Metoda HPLC s čiščenjem z imunoafinitetno kolono in fluorescenčno detekcijo po predkolonski derivatizaciji

Ta tehnična specifikacija opredeljuje metodo za določevanje fumonizina B1 in fumonizina B2 v predelani hrani na podlagi koruze za dojenčke in majhne otroke z metodo s tekočinsko kromatografijo visoke ločljivosti (HPLC) s čiščenjem z imunoafinitetno kolono in fluorescenčno detekcijo. Ta metoda je bila potrjena v medlaboratorijski študiji z analizami naravno kontaminiranih vzorcev in vzorcev z internimi dodatki v razponu od 111,6 μg/kg do 458,0 μg/kg za FB1+FB2, 89,1 µg/kg do 384,4 µg/kg za FB1 in 22,5 µg/kg do 73,6 µg/kg za FB2.
Za dodatne informacije o potrjevanju glej točko 8 in dodatek B.

General Information

Status
Withdrawn
Publication Date
05-Apr-2011
Withdrawal Date
09-Jun-2015
Current Stage
9960 - Withdrawal effective - Withdrawal
Completion Date
10-Jun-2015

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Standards Content (Sample)

SLOVENSKI STANDARD
SIST-TS CEN/TS 16187:2011
01-julij-2011
äLYLOD'RORþHYDQMHIXPRQL]LQD%LQIXPRQL]LQD%YSUHGHODQLKKUDQLQDRVQRYL
NRUX]H]DGRMHQþNHLQPDMKQHRWURNH0HWRGD+3/&VþLãþHQMHP]LPXQRDILQLWHWQR
NRORQRLQIOXRUHVFHQþQRGHWHNFLMRSRSUHGNRORQVNLGHULYDWL]DFLML
Foodstuffs - Determination of fumonisin B1 and fumonisin B2 in processed maize
containing foods for infants and young children - HPLC method with immunoaffinity
column cleanup and fluorescence detection after precolumn derivatization
Lebensmittel - Bestimmung von Fumonisin B1 und Fumonisin B2 in Säuglings- und
Kleinkindernahrung auf Maisbasis - HPLC-Verfahren mit Reinigung an einer
Immunoaffinitätssäule und Fluoreszenzdetektion nach Vorsäulenderivatisierung
Produits alimentaires - Fumonisine B1 et B2 dans les aliments à base de maïs pour
bébés et jeunes enfants
Ta slovenski standard je istoveten z: CEN/TS 16187:2011
ICS:
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
67.230 Predpakirana in pripravljena Prepackaged and prepared
hrana foods
SIST-TS CEN/TS 16187:2011 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST-TS CEN/TS 16187:2011

---------------------- Page: 2 ----------------------

SIST-TS CEN/TS 16187:2011


TECHNICAL SPECIFICATION
CEN/TS 16187

SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION
April 2011
ICS 67.230
English Version
Foodstuffs - Determination of fumonisin B1 and fumonisin B2 in
processed maize containing foods for infants and young children
- HPLC method with immunoaffinity column cleanup and
fluorescence detection after precolumn derivatization
Denrées alimentaires - Dosage de la fumonisine B1 et de la Lebensmittel - Bestimmung von Fumonisin B1 und
fumonisine B2 dans les aliments pour nourrissons et
Fumonisin B2 in Säuglings- und Kleinkindernahrung auf
jeunes enfants contenant du maïs transformé - Méthode Maisbasis - HPLC-Verfahren mit Reinigung an einer
par CLHP avec purification sur colonne d'immunoaffinité et Immunoaffinitätssäule und Fluoreszenzdetektion nach
détection de fluorescence après dérivation précolonne Vorsäulenderivatisierung
This Technical Specification (CEN/TS) was approved by CEN on 22 February 2011 for provisional application.

The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the CEN/TS can be converted into a European Standard.

CEN members are required to announce the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available
promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS)
until the final decision about the possible conversion of the CEN/TS into an EN is reached.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2011 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TS 16187:2011: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST-TS CEN/TS 16187:2011
CEN/TS 16187:2011 (E)
Contents Page
Foreword .3
1 Scope .4
2 Normative references .4
3 Principle .4
4 Reagents .4
5 Apparatus .7
6 Procedure .8
6.1 Extraction .
...

SLOVENSKI STANDARD
kSIST-TS FprCEN/TS 16187:2010
01-december-2010
äLYLOD'RORþHYDQMHIXPRQL]LQD%LQIXPRQL]LQD%YSUHGHODQLKNRUX]QLKNDãLFDK
]DGRMHQþNHLQPDMKQHRWURNH+3/&PHWRGDVþLãþHQMHP]LPXQRDILQLWHWQR
NRORQRLQIOXRUHVFHQþQRGHWHNFLMRSRSUHGNRORQVNLGHULYDWL]DFLML
Foodstuffs - Determination of fumonisin B1 and fumonisin B2 in processed maize
containing foods for infants and young children - HPLC method with immunoaffinity
column cleanup and fluorescence detection after precolumn derivatization
Lebensmittel - Bestimmung von Fumonisin B1 und Fumonisin B2 in Säuglings- und
Kleinkindernahrung auf Maisbasis - HPLC-Verfahren mit Reinigung an einer
Immunoaffinitätssäule und Fluoreszenzdetektion nach Vorsäulenderivatisierung
Produits alimentaires - Fumonisine B1 et B2 dans les aliments à base de maïs pour
bébés et jeunes enfants
Ta slovenski standard je istoveten z: FprCEN/TS 16187
ICS:
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
67.230 Predpakirana in pripravljena Prepackaged and prepared
hrana foods
kSIST-TS FprCEN/TS 16187:2010 en,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

kSIST-TS FprCEN/TS 16187:2010

---------------------- Page: 2 ----------------------

kSIST-TS FprCEN/TS 16187:2010


TECHNICAL SPECIFICATION
FINAL DRAFT
FprCEN/TS 16187
SPÉCIFICATION TECHNIQUE

TECHNISCHE SPEZIFIKATION

October 2010
ICS
English Version
Foodstuffs - Determination of fumonisin B and fumonisin B in
1 2
processed maize containing foods for infants and young children
- HPLC method with immunoaffinity column cleanup and
fluorescence detection after precolumn derivatization
Produits alimentaires - Fumonisine B et B dans les Lebensmittel - Bestimmung von Fumonisin B und
1 2 1
aliments à base de maïs pour bébés et jeunes enfants Fumonisin B in Säuglings- und Kleinkindernahrung auf
2
Maisbasis - HPLC-Verfahren mit Reinigung an einer
Immunoaffinitätssäule und Fluoreszenzdetektion nach
Vorsäulenderivatisierung


This draft Technical Specification is submitted to CEN members for Technical Committee Approval. It has been drawn up by the Technical
Committee CEN/TC 275.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.



Warning : This document is not a Technical Specification. It is distributed for review and comments. It is subject to change without notice
and shall not be referred to as a Technical Specification.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2010 CEN All rights of exploitation in any form and by any means reserved Ref. No. FprCEN/TS 16187:2010: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

kSIST-TS FprCEN/TS 16187:2010
FprCEN/TS 16187:2010 (E)
Contents Page
Foreword .3
1 Scope .4
2 Normative references .4
3 Principle .4
4 Reagents .4
5 Apparatus .7
6 Procedure .8
7 Calculation . 10
8 Precision . 11
9 Test report . 12
Annex A (informative) Typical chromatograms .
...

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