ISO 22174
(Main)Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection and quantification of microorganisms — General requirements and definitions
Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection and quantification of microorganisms — General requirements and definitions
Microbiologie de la chaîne alimentaire — Réaction de polymérisation en chaîne (PCR) pour la recherche et la quantification de micro-organismes — Exigences générales et définitions
General Information
Relations
Buy Standard
Standards Content (Sample)
FINAL DRAFT
International
Standard
ISO/FDIS 22174
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Polymerase chain reaction (PCR)
Voting begins on:
for the detection and quantification
2024-04-25
of microorganisms — General
Voting terminates on:
requirements and definitions
2024-06-20
Microbiologie de la chaîne alimentaire — Réaction de
polymérisation en chaîne (PCR) pour la recherche et la
quantification de micro-organismes — Exigences générales et
définitions
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
ISO/CEN PARALLEL PROCESSING LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 22174:2024(en) © ISO 2024
---------------------- Page: 1 ----------------------
FINAL DRAFT
ISO/FDIS 22174:2024(en)
International
Standard
ISO/FDIS 22174
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Polymerase chain reaction (PCR)
Voting begins on:
for the detection and quantification
of microorganisms — General
Voting terminates on:
requirements and definitions
Microbiologie de la chaîne alimentaire — Réaction de
polymérisation en chaîne (PCR) pour la recherche et la
quantification de micro-organismes — Exigences générales et
définitions
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
COPYRIGHT PROTECTED DOCUMENT
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2024
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO
ISO/CEN PARALLEL PROCESSING
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ISO/FDIS 22174:2024(en) © ISO 2024
© ISO 2024 – All rights reserved
ii
---------------------- Page: 2 ----------------------
ISO/FDIS 22174:2024(en)
Contents Page
Foreword .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
3.1 General terms .2
3.2 Terms related to the extraction and purification of DNA/RNA .3
3.3 Terms related to reverse transcription of RNA to DNA .4
3.4 Terms related to DNA amplification by PCR/RT-PCR .4
3.5 Terms related to controls .5
3.6 Terms related to qPCR .6
3.7 T
...
ISO/FDIS 22174:2024(E)
Date: 2024-02-27
ISO/TC 34/SC 9
Secretariat: AFNOR
Date: 2024-04-10
Microbiology of the food chain — Polymerase chain reaction (PCR)
for the detection and quantification of microorganisms — General
requirements and definitions
Microbiologie de la chaîne alimentaire — Réaction de polymérisation en chaîne (PCR) pour la recherche et la
quantification de micro-organismes — Exigences générales et définitions
FDIS stage
---------------------- Page: 1 ----------------------
© ISO 2022 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO Copyright Office copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
Email: E-mail: copyright@iso.org
Website: www.iso.org
Published in Switzerland.
---------------------- Page: 2 ----------------------
ISO/FDIS 22174:2024(en)
Contents
Foreword . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
3.1 General terms . 2
3.2 Terms related to the extraction and purification of DNA/RNA . 4
3.3 Terms related to reverse transcription of RNA to DNA . 4
3.4 Terms related to DNA amplification by PCR/RT-PCR . 4
3.5 Terms related to controls . 6
3.6 Terms related to qPCR . 7
3.7 Terms related to dPCR . 8
4 Principle . 9
4.1 General . 9
4.2 Laboratory sample . 9
4.3 Sampling, transport and storage . 10
4.4 Preparation of test sample . 10
5 Microbial enrichment and virus concentration . 10
5.1 Microbial enrichment . 10
5.2 Virus concentration . 10
6 Nucleic acid preparation . 10
6.1 General . 10
6.2 Prevention of amplification of DNA from dead cells . 11
6.3 Nucleic acid extraction, release and purification . 11
6.4 Nucleic acid quality and quantity . 11
7 P
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.