ISO 4730:2025
(Main)Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
This document specifies certain characteristics of the essential oil of Melaleuca, terpinen-4-ol type (tea tree oil), in order to facilitate assessment of its quality.
Huile essentielle de Melaleuca, type terpinén-4-ol (huile essentielle de Tea Tree)
ISO 4730:2017 spécifie certaines caractéristiques de l'huile essentielle de Melaleuca, type terpinèn-4-ol (huile essentielle de «Tea Tree»), destinées à faciliter l'évaluation de sa qualité.
General Information
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Standards Content (Sample)
International
Standard
ISO 4730
Fourth edition
Essential oil of Melaleuca, terpinen-
2025-06
4-ol type (tea tree oil)
Huile essentielle de Melaleuca, type terpinén-4-ol (huile
essentielle de Tea Tree)
Reference number
© ISO 2025
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Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements . 1
4.1 General .1
4.2 Chromatographic profile .2
5 Additional information . 2
5.1 Flash point .2
5.2 Enantiomeric distribution .3
6 Sampling . 3
7 Packaging, labelling, marking and storage . 3
Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the
essential oil of Melaleuca, terpinen-4-ol type (tea tree oil) . 4
Annex B (informative) Flash point. 7
Annex C (informative) Enantiomeric distribution — General information . 8
Bibliography . 9
iii
Foreword
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This document was prepared by Technical Committee ISO/TC 54, Essential oils.
This fourth edition cancels and replaces the third edition (ISO 4730:2017), which has been technically
revised. It also incorporates the Amendment ISO 4730:2017/Amd 1:2018.
The main changes are as follows:
— inclusion of enantiomeric distribution parameters for α-terpineol.
Any feedback or questions on this document should be directed to the user’s national standards body. A
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International Standard ISO 4730:2025(en)
Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
1 Scope
This document specifies certain characteristics of the essential oil of Melaleuca, terpinen-4-ol type (tea tree
oil), in order to facilitate assessment of its quality.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 210, Essential oils — General requirements and guidelines for packaging, conditioning and storage
ISO 211, Essential oils — General requirements for labelling and marking of containers
ISO 212, Essential oils — Sampling
ISO 279, Essential oils — Determination of relative density at 20 °C — Reference method
ISO 280, Essential oils — Determination of refractive index
ISO 592, Essential oils — Determination of optical rotation
ISO 875, Essential oils — Evaluation of miscibility in ethanol
ISO 11024 (all parts), Essential oils — General guidance on chromatographic profiles
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
essential oil of Melaleuca, terpinen-4-ol type
tea tree oil
essential oil obtained by steam distillation of the leaves and terminal branchlets of Melaleuca alternifolia
(Maiden and Betche) Cheel or of M. linariifolia Sm
Note 1 to entry: For information on the characterization of this essential oil, see ISO/TR 21092.
4 Requirements
4.1 General
Essential oil of Melaleuca, terpinen-4-ol type (tea tree oil)
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