SIST EN ISO 6888-2:2021
(Main)Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using rabbit plasma fibrinogen agar medium (ISO 6888-2:2021)
Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using rabbit plasma fibrinogen agar medium (ISO 6888-2:2021)
This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]).
This document is applicable to:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
This horizontal method was originally developed for the examination of all samples belonging to the food chain.
Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method.
Based on the information available at the time of publication of this document, this method is particularly suitable for the examination of fermented products or other products containing technological microbiota based on Staphylococcus spp. (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by:
— staphylococci forming atypical colonies on a Baird-Parker agar medium;
— background microbiota that can obscure the colonies being sought.
Nevertheless, both ISO 6888-1 and this document are given equivalent status.
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für die Zählung von koagulase-positiven Staphylokokken (Staphylococcus aureus und andere Spezies) - Teil 2: Verfahren mit Kaninchenplasma/Fibrinogen-Agar (ISO 6888-2:2021)
Dieses Dokument legt ein horizontales Verfahren für die Zählung von koagulase-positiven Staphylokokken durch die Zählung von Kolonien fest, die nach aerober Bebrütung bei 34 °C bis 38 °C und Koagulase-Bestätigung auf einem festen Medium (Kaninchenplasma-Fibrinogen-Agar-Medium) erhalten wurden (siehe Literaturhinweis [10]).
Dieses Dokument ist anwendbar für:
- Erzeugnissen, die für den menschlichen Verzehr vorgesehen sind;
- Erzeugnissen, die als Futtermittel vorgesehen sind;
- Umgebungsproben im Bereich der Herstellung und Handhabung von Lebensmitteln und Futtermitteln; und
- Proben aus dem Bereich der Primärproduktion.
Dieses horizontale Verfahren wurde ursprünglich dazu entwickelt, alle Proben, die zu einer Lebensmittelkette zählen, zu überprüfen.
Wegen der Vielzahl der Erzeugnisse in der Lebensmittelkette ist es möglich, dass dieses horizontale Verfahren für alle Erzeugnisse nicht bis ins Detail geeignet ist. Dennoch wird erwartet, dass die erforderlichen Modifikationen derart gering gehalten werden, dass sie nicht zu einer erheblichen Abweichung von diesem horizontalen Verfahren führen.
Entsprechend den zum Zeitpunkt der Veröffentlichung dieses Dokuments verfügbaren Informationen gilt dieses Verfahren nicht als (vollständig) geeignet für die Untersuchung von fermentierten Erzeugnissen oder sonstigen Erzeugnissen, die eine auf Staphylococcus spp basierende technologische Flora enthalten (z. B. S. xylosus) (wie z. B. Rohmilchkäse und bestimmte rohe Fleischerzeugnisse), die wahrscheinlich kontaminiert sind durch:
- Staphylokokken, die auf Baird-Parker-Agar-Medium atypische Kolonien bilden; und
- Begleitflora, die die gesuchten Kolonien überdecken kann.
Trotzdem sind ISO 6888 1 und dieses Dokument gleichwertig.
WARNUNG - Zum Schutz der Gesundheit des Laborpersonals ist es wesentlich, die Prüfungen zur Zählung von Staphylokokken nur in angemessen ausgerüsteten Laboren durchzuführen, wobei die Prüfung unter der Leitung eines erfahrenen Mikrobiologen erfolgt, und dass bei der Entsorgung von bebrüteten Materialien mit großer Sorgfalt vorgegangen wird. Anwender dieses Dokuments sollten mit der üblichen Laborpraxis vertraut sein. Dieses Dokument gibt nicht vor, alle unter Umständen mit der Anwendung des Verfahrens verbundenen Sicherheitsaspekte anzusprechen. Es liegt in der Verantwortlichkeit des Anwenders, geeignete Sicherheits- und Gesundheitsmaßnahmen festzulegen.
Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des staphylocoques à coagulase positive (Staphylococcus aureus et autres espèces) - Partie 2 : Méthode utilisant le milieu gélosé au plasma de lapin et au fibrinogène (ISO 6888-2:2021)
Le présent document spécifie une méthode horizontale de dénombrement des staphylocoques à coagulase positive par comptage des colonies obtenues en milieu solide (milieu gélosé au plasma de lapin et au fibrinogène) après incubation en aérobiose entre 34 °C et 38 °C (voir la Référence [10]).
Le présent document s’applique aux:
— produits destinés à la consommation humaine;
— produits destinés à l’alimentation des animaux;
— échantillons environnementaux prélevés dans les secteurs de la production et de la distribution des aliments; et
— échantillons prélevés au stade de production primaire.
Cette méthode horizontale a été élaborée à l’origine pour l’examen de tous les échantillons appartenant à la chaîne alimentaire.
En raison de la grande diversité des produits de la chaîne alimentaire, il est possible que cette méthode horizontale ne soit pas entièrement appropriée à tous les produits. Néanmoins, il est attendu que les modifications requises soient réduites le plus possible afin de ne pas s’écarter de manière significative de cette méthode horizontale.
D’après les informations disponibles au moment de la publication du présent document, il convient de recommander cette méthode pour l’examen des produits fermentés ou d’autres produits contenant une flore technologique à base de Staphylococcus spp. (par exemple, S. xylosus) (tels que les fromages à base de lait cru et certains produits à base de viande crue) susceptibles d’être contaminés par:
— des staphylocoques formant des colonies non caractéristiques sur un milieu gélosé de Baird-Parker;
— une flore annexe pouvant masquer les colonies recherchées.
Néanmoins, l’ISO 6888-1 et le présent document bénéficient d’un statut équivalent.
Mikrobiologija v prehranski verigi - Horizontalna metoda za štetje koagulazno pozitivnih stafilokokov (Staphylococcus aureus in drugih vrst) - 2. del: Metoda uporabe agarja z zajčjo plazmo iz fibrinogenov (ISO 6888-2:2021)
Ta dokument določa horizontalno metodo za štetje koagulazno pozitivnih stafilokokov
s štetjem kolonij, pridobljenih z uporabo trdnega medija (agar z zajčjo plazmo s fibrinogeni) po aerobni inkubaciji pri temperaturi med 34 °C in 38 °C (glej vir [10]).
Ta dokument se uporablja za:
– proizvode, namenjene za prehrano ljudi;
– proizvode, namenjene za živalsko krmo;
– okoljske vzorce v območju proizvodnje in ravnanja z živili in krmo;
– vzorce iz faze primarne proizvodnje.
Ta horizontalna metoda je bila prvotno razvita za preiskovanje vseh vzorcev, ki pripadajo
prehranski verigi.
Zaradi velike raznolikosti proizvodov v prehranski verigi morda ta horizontalna metoda v vseh podrobnostih ni v celoti primerna za vse proizvode. Vseeno gre pričakovati, da je število zahtevanih sprememb manjše, s tem pa ni bistvenih odstopanj od te horizontalne metode.
Na podlagi informacij, ki so bile na voljo v času objave tega dokumenta, ta metoda ne
velja za (povsem) primerno za preiskovanje fermentiranih proizvodov ali drugih proizvodov, ki vsebujejo
tehnološko floro na osnovi bakterije Staphylococcus spp. (npr. S. xylosus) (npr. sir iz surovega mleka in določeni izdelki iz surovega mesa) in so lahko kontaminirani s/z:
– bakterijo staphylococci, ki razvije atipične kolonije na Baird-Parkerjevem agarju;
– zaledno floro, ki lahko skrije iskane kolonije.
Vseeno imata standard ISO 6888-1 in ta dokument enakovreden status.
General Information
Relations
Overview
EN ISO 6888-2:2021 - "Microbiology of the food chain - Horizontal method for the enumeration of coagulase‑positive staphylococci - Part 2" specifies a horizontal enumeration method for coagulase‑positive staphylococci (notably Staphylococcus aureus) using rabbit plasma fibrinogen agar (RPFA). The method counts colonies on RPFA after aerobic incubation at 34 °C to 38 °C and is intended for testing materials across the food chain, including food and feed products, environmental samples in production/handling areas, and primary production samples.
Key topics and requirements
- Method principle: enumeration of coagulase‑positive staphylococci by colony counting on RPFA medium under aerobic incubation.
- Incubation conditions: aerobic incubation at 34–38 °C; standard incubation time referenced as 24 h ± 2 h where applicable.
- Culture medium: use of rabbit plasma fibrinogen agar (RPFA) with defined performance testing (see Annex B).
- Preparation and procedure: test portion, initial suspension, serial dilutions, inoculation, incubation and colony counting workflow (flow diagram in Annex A).
- Performance evaluation: interlaboratory study results, repeatability and reproducibility limits included for method validation.
- Quality and standards alignment: method aligned with ISO 7218 laboratory practice and requires consideration of ISO 11133 for culture media preparation/performance. References to sampling standards and updated bibliography are included.
- Scope limitations: designed as a horizontal method applicable across diverse food-chain matrices; some products (e.g., strong background microbiota or atypical colony formers) may require minimal, justified adaptations.
- Safety: warning that testing must be performed in properly equipped labs under skilled supervision with appropriate biosafety and waste disposal.
Applications and users
- Who uses it: food and feed microbiology laboratories, quality control teams in dairy, meat and fermented‑product industries, regulatory and surveillance labs, and academic or contract testing facilities.
- Practical applications:
- Routine monitoring of Staphylococcus aureus counts in food and feed.
- Environmental monitoring in processing and handling areas.
- Examination of fermented products and raw‑milk cheeses or raw meat products where technological staphylococci (e.g., S. xylosus) and atypical colonies occur.
- Cases where Baird‑Parker agar may not clearly differentiate target colonies due to background flora.
Related standards
- ISO 6888‑1 (detection/enumeration - equivalent status for many uses)
- ISO 6888‑3 (other methods in the series)
- ISO 7218 (general microbiological laboratory practice)
- ISO 11133 (media preparation and performance testing)
- Relevant national sampling standards and ISO sampling references
This standard is essential for reliable, standardized enumeration of coagulase‑positive staphylococci in the food chain and supports compliance, product safety assessment, and HACCP/environmental monitoring programs.
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2021
Nadomešča:
SIST EN ISO 6888-2:1999
SIST EN ISO 6888-2:1999/A1:2003
Mikrobiologija v prehranski verigi - Horizontalna metoda za štetje koagulazno
pozitivnih stafilokokov (Staphylococcus aureus in drugih vrst) - 2. del: Metoda
uporabe agarja z zajčjo plazmo iz fibrinogenov (ISO 6888-2:2021)
Microbiology of the food chain - Horizontal method for the enumeration of coagulase-
positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using
rabbit plasma fibrinogen agar medium (ISO 6888-2:2021)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für die Zählung von
koagulase-positiven Staphylokokken (Staphylococcus aureus und andere Spezies) - Teil
2: Verfahren mit Kaninchenplasma/Fibrinogen-Agar (ISO 6888-2:2021)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des
staphylocoques à coagulase positive (Staphylococcus aureus et autres espèces) - Partie
2 : Méthode utilisant le milieu gélosé au plasma de lapin et au fibrinogène (ISO 6888-
2:2021)
Ta slovenski standard je istoveten z: EN ISO 6888-2:2021
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 6888-2
EUROPEAN STANDARD
NORME EUROPÉENNE
September 2021
EUROPÄISCHE NORM
ICS 07.100.30 Supersedes EN ISO 6888-2:1999, EN ISO 6888-
2:1999/A1:2003
English Version
Microbiology of the food chain - Horizontal method for the
enumeration of coagulase-positive staphylococci
(Staphylococcus aureus and other species) - Part 2:
Method using rabbit plasma fibrinogen agar medium (ISO
6888-2:2021)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour le dénombrement des staphylocoques Verfahren für die Zählung von koagulase-positiven
à coagulase positive (Staphylococcus aureus et autres Staphylokokken (Staphylococcus aureus und andere
espèces) - Partie 2 : Méthode utilisant le milieu gélosé Spezies) - Teil 2: Verfahren mit
au plasma de lapin et au fibrinogène (ISO 6888- Kaninchenplasma/Fibrinogen-Agar (ISO 6888-2:2021)
2:2021)
This European Standard was approved by CEN on 27 April 2021.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6888-2:2021 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 6888-2:2021) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food chain” the
secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by March 2022, and conflicting national standards shall
be withdrawn at the latest by March 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 6888-2:1999.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN websites.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 6888-2:2021 has been approved by CEN as EN ISO 6888-2:2021 without any
modification.
INTERNATIONAL ISO
STANDARD 6888-2
Second edition
2021-08
Microbiology of the food chain —
Horizontal method for the
enumeration of coagulase-positive
staphylococci (Staphylococcus aureus
and other species) —
Part 2:
Method using rabbit plasma
fibrinogen agar medium
Microbiologie de la chaîne alimentaire — Méthode horizontale
pour le dénombrement des staphylocoques à coagulase positive
(Staphylococcus aureus et autres espèces) —
Partie 2: Méthode utilisant le milieu gélosé au plasma de lapin et au
fibrinogène
Reference number
ISO 6888-2:2021(E)
©
ISO 2021
ISO 6888-2:2021(E)
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2021 – All rights reserved
ISO 6888-2:2021(E)
Contents Page
Foreword .iv
Introduction .vi
1 Scope . 1
2 Normative references . 2
3 Terms and definitions . 2
4 Principle . 2
4.1 General . 2
4.2 Incubation . 2
4.3 Enumeration . 3
5 Culture media and reagents . 3
6 Equipment and consumables . 3
7 Sampling . 4
8 Preparation of the test sample . 4
9 Procedure (see Figure A.1) . 4
9.1 Test portion, initial suspension and dilutions . 4
9.2 Inoculation and incubation . 4
9.3 Counting of colonies . 5
9.3.1 General description of colonies growing on RPFA medium . 5
9.3.2 Colony counting procedure . 5
10 Expression of results . 5
11 Performance characteristics of the method . 5
11.1 Interlaboratory study . 5
11.2 Repeatability limit . 5
11.3 Reproducibility limit . 6
12 Test report . 7
13 Quality assurance . 7
Annex A (normative) Flow diagram of the procedure . 8
Annex B (normative) Culture media and reagents . 9
Annex C (informative) Results of the interlaboratory study .12
Bibliography .14
ISO 6888-2:2021(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology, in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 463, Microbiology of the food chain, in accordance with the Agreement on technical
cooperation between ISO and CEN (Vienna Agreement).
This second edition cancels and replaces the first edition (ISO 6888-2:1999), which has been technically
revised. It also incorporates the Amendment ISO 6888-2:1999/Amd 1:2003. The main changes compared
with the previous edition are as follows:
— the title has been changed to relate to the “food chain”;
— the status of ISO 6888-1 and this document has been clarified;
— the document has been aligned with ISO 7218:2007, i.e. and pour molten agar medium at 44 °C to
47 °C;
— all occurrences, when appropriate, have been changed from “35 °C or 37 °C” to “34 °C to 38 °C”;
— all occurrences of incubation time, when appropriate, have been changed from “18 h to 24 h” to
“24 h ± 2 h”;
— requirements have been added to use ISO 11133;
— all available standards related to sampling techniques have been updated;
— flow diagram procedure in Annex A has been updated;
— culture media and reagents with performance testing have been added and moved to Annex B;
— performance testing for rabbit plasma fibrinogen agar (RPFA) medium has been added;
— results of the interlaboratory study (from ISO 6888-2:1999/Amendment 1:2003 Precision data)
have been updated;
iv © ISO 2021 – All rights reserved
ISO 6888-2:2021(E)
— the Bibliography has been updated.
A list of all parts in the ISO 6888 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
ISO 6888-2:2021(E)
Introduction
ISO 6888-1, this document and ISO 6888-3 describe three horizontal methods for the detection
and enumeration of coagulase-positive staphylococci among which enterotoxinogenic strains are
encountered. It is mainly concerned with Staphylococcus aureus, but also with S. intermedius and certain
strains of S. hyicus.
For the purposes of this document, the characterization of staphylococci is based on a positive coagulase
reaction, but it is recognized that some strains of Staphylococcus aureus give weakly positive coagulase
reactions. These latter strains can be confused with other bacteria but they can be distinguished by the
use of additional tests not included in this document, such as tests for sensitivity to lysostaphin, and for
production of haemolysin, thermostable nuclease and acid from mannitol (see ISO 7218 and Reference
[13]).
The main technical changes listed in the Foreword, introduced in this document compared with the
previous edition, are considered as minor (see ISO 17468). They have a minor impact on the performance
characteristics of the method.
The results of the interlaboratory study and samples tested are described in Annex C.
vi © ISO 2021 – All rights reserved
INTERNATIONAL STANDARD ISO 6888-2:2021(E)
Microbiology of the food chain — Horizontal method
for the enumeration of coagulase-positive staphylococci
(Staphylococcus aureus and other species) —
Part 2:
Method using rabbit plasma fibrinogen agar medium
WARNING — In order to safeguard the health of laboratory personnel, it is essential that tests
for enumerating staphylococci are only undertaken in properly equipped laboratories, under
the control of a skilled microbiologist and that great care is taken in the disposal of all incubated
materials. Persons using this document should be familiar with normal laboratory practice.
This document does not purport to address all of the safety aspects, if any, associated with its
use. It is the responsibility of the user to establish appropriate safety and health practices.
1 Scope
This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci
by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after
aerobic incubation at 34 °C to 38 °C (see Reference [10]).
This document is applicable to:
— products intended for human consumption;
— products intended for animal feeding;
— environmental samples in the area of food and feed production and handling;
— samples from the primary production stage.
This horizontal method was originally developed for the examination of all samples belonging to the
food chain.
Because of the large variety of products in the food chain, it is possible that this horizontal method is not
appropriate in every detail for all products. Nevertheless, it is expected that the required modifications
are minimized so that they do not result in a significant deviation from this horizontal method.
Based on the information available at the time of publication of this document, this method is not
considered to be (fully) suited to the examination of fermented products or other products containing
technological flora based on Staphylococcus spp. (e.g. S. xylosus) (such as cheeses made from raw milk
and certain raw meat products) likely to be contaminated by:
— staphylococci forming atypical colonies on a Baird-Parker agar medium;
— background flora that can obscure the colonies being sought.
Nevertheless, both ISO 6888-1 and this document are given equivalent status.
ISO 6888-2:2021(E)
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 6887 (all parts), Microbiology of the food chain — Preparation of test samples, initial suspension and
decimal dilutions for microbiological examination
ISO 7218, Microbiology of food and animal feeding stuffs — General requirements and guidance for
microbiological examinations
ISO 11133, Microbiology of food, animal feed and water — Preparation, production, storage and
performance testing of culture media
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
3.1
coagulase-positive staphylococci
bacteria that form typical colonies in a selective culture medium (rabbit plasma fibrinogen agar
medium)
Note 1 to entry: The typical colonies are described in 9.3.
3.2
enumeration of coagulase-positive staphylococci
determination of the number of coagulase-positive staphylococci (3.1) per gram, per millilitre, per square
centimetre or per sampling device/sampled area.
Note 1 to entry: A sampled area is an area not defined by a numerical size, for example, a hot tap, a door handle.
4 Principle
4.1 General
Preparation of poured plate of the rabbit plasma fibrinogen agar medium, with a specified quantity of
the test sample if the product is liquid or with a specified quantity of the initial suspension in the case
of other products.
Inoculation, under the same conditions, using decimal dilutions of the test sample, follow the
procedure(s) in accordance with ISO 7218.
NOTE The volume of rabbit plasma fibrinogen agar medium to be added to the inoculum is a critical point for
the coagulase method and reaction.
4.2 Incubation
Aerobic incubation of the plates at 34 °C to 38 °C and examination after 24 h and, if necessary, after
48 h.
2 © ISO 2021 – All rights reserved
ISO 6888-2:2021(E)
4.3 Enumeration
Calculation of the number of coagulase-positive staphylococci per gram of sample, per millilitre of
sample, per square centimetre or per sampling device from the number of typical colonies obtained on
plates at dilution levels chosen to give a significant result within the counting limits of the method and
in accordance with ISO 7218.
NOTE See Annex A for a flow diagram.
5 Culture media and reagents
Follow current laboratory practices in accordance with ISO 7218.
The composition of culture media and reagents and their preparation are specified in Annex B.
For performance testing of culture media and reagents, follow the procedures in accordance with either
Annex B or ISO 11133, or both.
For diluent(s), see the relevant part of the ISO 6887 series.
6 Equipment and consumables
Disposable equipment is an acceptable alternative to reusable glassware if it has suitable specifications.
Usual microbiological laboratory equipment (see ISO 7218) and, in particular, the following.
6.1 Apparatus for dry sterilization (oven) and wet sterilization (autoclave).
See ISO 7218.
6.2 Incubator, capable of maintaining the inoculated media, within the temperature range 34 °C to
38 °C.
NOTE The range 34 °C to 38 °C for incubation of media includes the use of incubators set at 35 °C ± 1 °C,
36 °C ± 2 °C or 37 °C ± 1 °C.
6.3 Water bath, or similar apparatus, capable of being maintained at 44 °C to 47 °C.
6.4 Sterile Petri dishes, w
...
Frequently Asked Questions
SIST EN ISO 6888-2:2021 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Microbiology of the food chain - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 2: Method using rabbit plasma fibrinogen agar medium (ISO 6888-2:2021)". This standard covers: This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]). This document is applicable to: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. Based on the information available at the time of publication of this document, this method is particularly suitable for the examination of fermented products or other products containing technological microbiota based on Staphylococcus spp. (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by: — staphylococci forming atypical colonies on a Baird-Parker agar medium; — background microbiota that can obscure the colonies being sought. Nevertheless, both ISO 6888-1 and this document are given equivalent status.
This document specifies a horizontal method for the enumeration of coagulase-positive staphylococci by counting the colonies obtained on a solid medium (rabbit plasma fibrinogen agar medium) after aerobic incubation at 34 °C to 38 °C (see Reference [10]). This document is applicable to: — products intended for human consumption; — products intended for animal feeding; — environmental samples in the area of food and feed production and handling; — samples from the primary production stage. This horizontal method was originally developed for the examination of all samples belonging to the food chain. Because of the large variety of products in the food chain, it is possible that this horizontal method is not appropriate in every detail for all products. Nevertheless, it is expected that the required modifications are minimized so that they do not result in a significant deviation from this horizontal method. Based on the information available at the time of publication of this document, this method is particularly suitable for the examination of fermented products or other products containing technological microbiota based on Staphylococcus spp. (e.g. S. xylosus) (such as cheeses made from raw milk and certain raw meat products) likely to be contaminated by: — staphylococci forming atypical colonies on a Baird-Parker agar medium; — background microbiota that can obscure the colonies being sought. Nevertheless, both ISO 6888-1 and this document are given equivalent status.
SIST EN ISO 6888-2:2021 is classified under the following ICS (International Classification for Standards) categories: 07.100.30 - Food microbiology. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 6888-2:2021 has the following relationships with other standards: It is inter standard links to SIST EN ISO 6888-2:1999/A1:2003, SIST EN ISO 6888-2:1999, SIST EN ISO 6888-2:2021/A1:2024. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase SIST EN ISO 6888-2:2021 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.
기사 제목: SIST EN ISO 6888-2:2021 - 식품 공급망의 미생물학 - 응응형 응고효소 양성 스태포옥코크스 염색군 지수 측정을 위한 수평방법 (스태포옥콧코스 우레아 가루(ISO 6888-2:2021) 활용 방법) 기사 내용: 이 문서는 수평방법에 따라 응고효소 양성 스태포옥코크스 군을 셀 수 있는 방법을 제시한다. 이는 고체 배지(스태포옥콧코크스 우레아 가루(ISO 6888-2:2021))에 얻은 콜로니를 미생물적으로 배양한 후 34°C에서 38°C의 공기 중에서 배양시킨다. 이 문서는 다음에 적용된다: - 인간의 소비를 위한 제품 - 동물 사료로 사용되는 제품 - 식품 및 사료 생산 및 처리 영역의 환경 샘플 - 1차 생산 단계의 샘플 이 수평 방법은 원래 식품 공급망에 속하는 모든 샘플에 대한 조사를 위해 개발되었다. 그러나 식품 공급망에는 다양한 제품이 있기 때문에 이 수평 방법이 모든 제품에 대해 모든 세부 사항에 적합하지 않을 수 있다. 그러나 필요한 수정 사항은 이 수평 방법과 크게 일차하지 않도록 최소화될 것으로 예상된다. 이 문서가 게시된 시점에서 제공된 정보를 기준으로 볼 때, 이 방법은 발효 제품 또는 스태포옥코크스 spp. (예: S. xylosus)를 기반으로한 기술적인 균주(원료 우유에서 만든 치즈 및 일부 원료 육류 제품과 같은)을 포함하는 다른 제품을 조사하기에는 적합하지 않다고 생각된다. 그러나 ISO 6888-1과 이 문서는 동등한 지위를 가진다.
記事のタイトル：SIST EN ISO 6888-2:2021 - 食品チェーンの微生物学 - 表面法による凝固酵素陽性のブドウ球菌（Staphylococcus aureusおよび他の種類）の数え上げのための方法 - ウサギプラズマフィブリノーゲン寒天培地を使用した方法（ISO 6888-2:2021） 記事内容：本文書では、34°Cから38°C（参照[10]を参照）で好気性培養した後、固体培地（ウサギプラズマフィブリノーゲン寒天培地）で得られたコロニーの数え上げにより、凝固酵素陽性のブドウ球菌を数えるための水平法を規定しています。 この文書は次の対象に適用されます： - 人間の摂取を意図した製品 - 動物の飼料として使用される製品 - 食品および飼料の生産および取扱いの環境サンプル - 初期生産段階のサンプル この水平法はもともと、食品チェーンに属するすべてのサンプルの調査のために開発されました。食品チェーンにはさまざまな製品があるため、この水平法がすべての製品のすべての詳細に適しているわけではないかもしれません。しかし、必要な修正はこの水平法からの大きな逸脱を生じないように最小限に抑えられることが期待されています。 本文書の発行時点で利用可能な情報に基づいて、この方法は発酵製品やStaphylococcus spp.に基づく技術的なフローラ（例：S. xylosus）を含む他の製品の調査には（完全に）適していないと考えられます。しかし、ISO 6888-1と本文書は同等の地位を持っています。
기사 제목: SIST EN ISO 6888-2:2021 - 식품 연쇄의 미생물학 - 응고양성 스태포칼슈균 (Staphylococcus aureus 및 기타 종)의 수적분석을 위한 평평한 방법 - 토끼 혈장 섬유소 원칙이를 활용한 방법 (ISO 6888-2:2021) 기사 내용: 이 문서는 공기배양 후 고형매체인 토끼 혈장 섬유소 원칙이가 포함된 배지에서 얻은 균사를 세는 것을 통해 응고양성 스태포칼슈균의 수적분석을 위한 평평한 방법을 규정한다. 이 문서는 인간의 소비를 목적으로 한 제품, 동물 사료를 목적으로 한 제품, 식품 및 사료 생산 및 처리 분야에서의 환경 샘플, 제1 생산 단계에서의 샘플에 적용된다. 이 평평한 방법은 원래 음식 연쇄에 속하는 모든 샘플의 조사를 위해 개발되었다. 음식 연쇄에는 다양한 제품이 포함되어 있기 때문에 이 평평한 방법이 모든 제품에 대해 모든 세부 사항에 적합하지 않을 수 있다. 그러나 필요한 수정사항이 최소화되어 이 평평한 방법과 중요한 차이를 가져오지 않도록 기대된다. 이 문서의 발표 시점에 제공된 정보를 기준으로 하여, 이 방법은 발효 제품 또는 기술적인 플로라를 포함한 다른 제품 (예: S. xylosus 등을 기반으로 하는 기타 제품, 원유 우유로 만든 치즈 및 특정 원육 제품)의 조사에 (완전히) 적합하지 않다고 여겨진다. 그러나 ISO 6888-1과 이 문서는 동등한 지위를 갖는다. 기사는 SIST EN ISO 6888-2:2021에 대해 설명하고 있으며, 이는 응고양성 스태포칼슈균의 수적분석을 위한 방법이다. 이 방법은 특정 온도에서 배양 후 균사를 세는 것을 포함한다. 이 방법은 인간과 동물용 제품뿐만 아니라 식품 및 사료 생산과 처리 관련 환경 샘플 및 제1 생산 단계의 샘플에 적용된다. 이 방법은 발효 제품이나 스태포칼슈균 등을 기반으로 하는 기술적인 플로라를 포함한 다른 제품에는 적합하지 않다고 설명되었다. 그러나 이 방법은 여전히 중요하며 ISO 6888-1과 동등한 지위를 가지고 있다.
記事タイトル：SIST EN ISO 6888-2:2021 - 食品チェーンの微生物学 - 凝固酵素陽性ブドウ球菌（Staphylococcus aureusおよびその他の種）の数え上げのための横断方法 - 兎血漿フィブリノーゲン寒天培地を用いる方法（ISO 6888-2:2021） 記事内容：この文書は、34℃から38℃の好気条件で培養した後に得られる固体培地（兎血漿フィブリノーゲン寒天培地）上の菌状コロニーを数えることにより、凝固酵素陽性ブドウ球菌（以下、コアグラゼ陽性スタフィロコッカス）の数え上げのための横断方法を規定しています。 この文書は以下に適用されます： - 人間の摂取を意図した製品 - 動物の餌料を意図した製品 - 食品および餌料の生産および取り扱いの領域の環境サンプル - 第一生産段階でのサンプル この横断方法はもともと、食品チェーンに属するすべてのサンプルの検査のために開発されました。しかし、食品チェーンにはさまざまな製品が含まれているため、この横断方法がすべての製品のすべての詳細に適しているわけではないかもしれません。ただし、必要な修正は最小限に抑えられることが期待されており、大幅な偏差を引き起こさないことが期待されています。 この文書の発行時点での情報に基づいて、この方法は発酵製品やスタフィロコッカス属（S. xylosusなど）に基づいた技術的なフローラを含む他の製品（生乳で作られたチーズや特定の生肉製品など）の調査には（完全に）適していないとされています。ただし、ISO 6888-1とこの文書は同等の地位を持っています。 記事は、SIST EN ISO 6888-2:2021について説明しており、食品チェーンにおける凝固酵素陽性ブドウ球菌の数え上げを行う方法であることがわかります。特定の温度で培養後に菌状コロニーを数えるという手法です。この方法は人間や動物用の製品に加えて、食品や餌料の生産・取り扱いに関わる環境サンプルや第一生産段階のサンプルに適用されます。ただし、発酵製品やスタフィロコッカスに基づく技術的なフローラを含む他の製品には適していないとされています。それにもかかわらず、この方法は重要であり、ISO 6888-1と同等の地位を持っているとされています。
The article discusses SIST EN ISO 6888-2:2021, which is a method for counting coagulase-positive staphylococci in food and environmental samples. The method involves using a solid medium and incubating it at a specific temperature. It is applicable to products intended for human and animal consumption, as well as samples from the production stage. While the method may not be suitable for all products in the food chain, it is expected that any necessary modifications will not significantly deviate from the original method. The article also mentions that the method may not be suitable for examining fermented products or products with technological flora based on Staphylococcus spp. Nevertheless, both ISO 6888-1 and this document hold the same status.
The article discusses the SIST EN ISO 6888-2:2021, a method for the enumeration of coagulase-positive staphylococci in various samples related to the food chain. It involves counting the colonies obtained on a solid medium after incubation at a specific temperature. The method is applicable to products for human and animal consumption, as well as environmental samples and those from the primary production stage. While this method is not suitable for fermented products or those containing technological flora based on Staphylococcus spp., it is still considered important and has equivalent status to ISO 6888-1.
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