SIST EN ISO 11290-1:2017/oprA1:2025

Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method - Amendment 1: Inclusion of storage of the samples before analysis and changes in the control strain for performance testing of culture media and reagents (ISO 11290 1:2017/DAM 1:2025)

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für den Nachweis und die Zählung von Listeria monocytogenes und von Listeria spp. - Teil 1: Nachweisverfahren - Änderung 1: Aufnahme der Lagerung der Proben vor der Analyse und Änderungen des Kontrollstamms für die Leistungsprüfung von Kulturmedien und Reagenzien (ISO 11290 1:2017/DAM 1:2025)

Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le dénombrement de Listeria monocytogenes et de Listeria spp. - Partie 1: Méthode de recherche - Amendement 1: Inclusion du stockage des échantillons avant analyse et modifications de la souche témoin pour les essais de performance des milieux de culture et des réactifs (ISO 11290 1:2017/DAM 1:2025)

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp. - 1. del: Metoda za ugotavljanje prisotnosti - Dopolnilo A1: Vključitev shranjevanja vzorcev pred analizo in sprememb kontrolnega seva za preskušanje učinkovitosti gojišč in reagentov (ISO 11290 1:2017/DAM 1:2025)

General Information

• Status: Not Published
• Public Enquiry End Date: 30-Jul-2025
• ICS: 07.100.30 - Food microbiology
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 4020 - Public enquire (PE) (Adopted Project)
• Start Date: 29-May-2025
• Due Date: 16-Oct-2025
• Completion Date: 05-Aug-2025
• Ref Project: EN ISO 11290-1:2017/prA1 - Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method - Amendment 1: Inclusion of storage of the samples before analysis and changes in the control strain for performance testing of culture media and reagents (ISO 11290 1:2017/DAM 1:2025)

Relations

• Amends: SIST EN ISO 11290-1:2017 - Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 1: Detection method (ISO 11290-1:2017)
• Effective Date: 17-Jul-2024

Standards Content (Sample)

SLOVENSKI STANDARD
01-julij-2025
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti in števila Listeria monocytogenes in Listeria spp. - 1. del: Metoda za
ugotavljanje prisotnosti - Dopolnilo A1: Vključitev shranjevanja vzorcev pred
analizo in sprememb kontrolnega seva za preskušanje učinkovitosti gojišč in
reagentov (ISO 11290 1:2017/DAM 1:2025)
Microbiology of the food chain - Horizontal method for the detection and enumeration of
Listeria monocytogenes and of Listeria spp. - Part 1: Detection method - Amendment 1:
Inclusion of storage of the samples before analysis and changes in the control strain for
performance testing of culture media and reagents (ISO 11290 1:2017/DAM 1:2025)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren für den Nachweis und die
Zählung von Listeria monocytogenes und von Listeria spp. - Teil 1: Nachweisverfahren -
Änderung 1: Aufnahme der Lagerung der Proben vor der Analyse und Änderungen des
Kontrollstamms für die Leistungsprüfung von Kulturmedien und Reagenzien (ISO 11290
1:2017/DAM 1:2025)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche et le
dénombrement de Listeria monocytogenes et de Listeria spp. - Partie 1: Méthode de
recherche - Amendement 1: Inclusion du stockage des échantillons avant analyse et
modifications de la souche témoin pour les essais de performance des milieux de culture
et des réactifs (ISO 11290 1:2017/DAM 1:2025)
Ta slovenski standard je istoveten z: EN ISO 11290-1:2017/prA1
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
Amendment
ISO 11290-1:2017/
DAM 1
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Horizontal method for the detection
and enumeration of Listeria
Voting begins on:
2025-05-19
monocytogenes and of Listeria
Voting terminates on:
spp. —
2025-08-11
Part 1:
Detection method
AMENDMENT 1: Inclusion of storage
of the samples before analysis and
changes in the control strain for
performance testing of culture media
and reagents
ICS: 07.100.30
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Reference number
ISO 11290-1:2017/DAM 1:2025(en)

DRAFT
ISO 11290-1:2017/DAM 1:2025(en)
Amendment
ISO 11290-1:2017/
DAM 1
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Horizontal method for the
detection and enumeration of
Voting begins on:
Listeria monocytogenes and of
Voting terminates on:
Listeria spp. —
Part 1:
Detection method
AMENDMENT 1: Inclusion of storage
of the samples before analysis and
changes in the control strain for
performance testing of culture media
and reagents
ICS: 07.100.30
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2025
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
BE CONSIDERED IN THE LIGHT OF THEIR
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
or ISO’s member body in the country of the requester.
NATIONAL REGULATIONS.
ISO copyright office
RECIPIENTS OF THIS DRAFT ARE INVITED
CP 401 • Ch. de Blandonnet 8
TO SUBMIT, WITH THEIR COMMENTS,
CH-1214 Vernier, Geneva
NOTIFICATION OF ANY RELEVANT PATENT
Phone: [phone]
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Email: [e-mail]
Website: www.iso.org
Published in Switzerland Reference number
ISO 11290-1:2017/DAM 1:2025(en)

ii
ISO 11290-1:2017/DAM 1:2025(en)
Foreword
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bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
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The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
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www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
A list of all parts in the ISO 11290 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iii
ISO 11290-1:2017/DAM 1:2025(en)
Microbiology of the food chain — Horizontal method for the
detection and enumeration of Listeria monocytogenes and of
Listeria spp. —
Part 1:
Detection method
AMENDMENT 1: Inclusion of storage of the samples before
analysis and changes in the control strain for performance testing
of culture media and reagents
Clause 7
Replace the text with the following:
7.  Sampling, transportation and storage of samples
Sampling is not part of the method specified in this document. If there is no specific International Standard
dealing with sampling of the product concerned, it is recommended that the parties concerned come to an
agreement on this subject. For food and feed samples, refer to ISO/TS 17728. For environmental samples,
use ISO 18593 and see Reference [24].
It is important that the laboratory receives a sample which is truly representative and has not been damaged
or changed during transport or storage (see ISO 7218).
For storage conditions before analysis, see ISO 7218. If ISO 7218 conditions can’t be respected, other
storage conditions may be used in which previously it has been demonstrated that Listeria spp./Listeria
monocytogenes contamination level won’t be modified. These conditions should be detailed and justified in
the test report.
9.5.2.5.2 and 9.5.2.6
Replace all instances of Listeria monocytogenes WDCM 00109 with Listeria monocytogenes WDCM 00231.
Add the following note after the last paragraph:
NOTE Supporting data for the replacement of Listeria monocytogenes WDCM 00109 by Listeria moncytogenes
WDCM 00231 are available at: https:// standards .iso .org/ iso/ 11290/ -1/ ed -2/ en/ amd/ 1

Clause B.5
Replace the text with the following:
B.5  Performance testing for the quality assurance of the culture media and reagents
The definition of productivity, specificity and selectivity are specified in ISO 11133. In general, follow the
procedures for performance testing described in ISO 11133. Table B.1 provides the performance testing

ISO 11290-1:2017/DAM 1:2025(en)
for the quality assurance of the culture media for the enrichment and isolation. Table B.2 provides the
performance testing for the quality assurance of the culture media and reagents for the confirmation tests.
For the second isolation medium, use performance testing according to the manufacturer’s specifications.
NOTE For productivity testing, in the case of detection of Listeria spp., it could be informative to add another
control strain (from another Listeria spp.).
Table B.1 — Performance testing for the quality assurance of the culture media
for the enrichment and isolation
Medium Function Incubation Control strains WDCM Reference Method of Criteria and
a
or rea- numbers media control characteristic
c
gent reactions
Listeria Good growth (2)
b
monocytogenes 00021 with at least
serovar 4b two streaks
with confluent
d
+ Escherichia coli
growth of blue
or 00013
green colonies
+ Enterococcus 00009
with opaque halo
d
faecalis or 00087
on Agar Listeria
Productivity — Qualitative
according to Ot-
Listeria
e
taviani and Agosti
monocytogenes 00231
Half- (25 ± 1) h/
in a three-phase
f serovars 1/2a, 3a
Fraser (30 ± 1) °C
streaking pattern
d
(also known as
+ Escherichia coli
or 00013
three-sector
+ Enterococcus 00009 streaking or
d
faecalis or 00087 T-streaking)
00012 < 10 colonies
d
Escherichia coli — Qualitative
or 00013 on TSA
Selectivity
Enterococcus 00009 < 100 colonies
— Qualitative
d
faecalis or 00087 on TSA
Listeria Good growth (2)
b
monocytogenes 00021 with at least two
serovar 4b streaks with
confluent growth
d
+ Escherichia coli
of blue green col-
or 00013
onies with opaque
+ Enterococcus 00009
halo on Agar
d
faecalis or 00087
Listeria according
(24 ± 2) h/
Fraser Productivity — Qualitative
to Ottaviani and
(37 ± 1) °C Listeria
e
Agosti
monocytogenes 00231
in a three-phase
serovars 1/2a, 3a
streaking pattern
d
+ Escherichia coli (also known as
or 00013
three-sector
+ Enterococcus 00009 streaking or
d
faecalis or 00087
T-streaking)
a
Refer to the reference strain catalogue on http:// www .wfcc .info for information on culture collection strain numbers and
contact details. WDCM: World Data Centre for Microorganisms.
b
Strains to be used as a minimum.
c
Growth is categorized as 0: no growth; 1: weak growth (partial inhibition); 2: good growth (see ISO 11133).
d
Strain free of choice; one of the strains has to be used as a minimum.
e
Strain optional.
f
Half-Fraser broth is preferably tested with the volume used in the analysis (e.g. 225 ml).
g
In case of both quantitative and qualitative use for the medium, only results of the quantitative tests are required.

ISO 11290-1:2017/DAM 1:2025(en)
TTabablele B B.11 ((ccoonnttiinnueuedd))
Medium Function Incubation Control strains WDCM Reference Method of Criteria and
a
or rea- numbers media control characteristic
c
gent reactions
00012 < 10 colonies
d
Escherichia coli — Qualitative
or 00013 on TSA
(24 ± 2) h/
Selectivity
(37 ± 1) °C
Enterococcus 00009 < 100 colonies
— Qualitative
d
faecalis or 00087 on TSA
Listeria
b
monocytogenes 00021
Good growth (2),
serovar 4b
blue green colo-
Productivity — Qualitative
nies with opaque
Listeria
e
halo
Agar
monocytogenes 00231
Listeria
serovars 1/2a, 3a
according (48 ± 4) h/
d
to Ottavi- (37 ± 1) °C Escherichia coli
or 00013
ani and
Selectivity — Qualitative Total inhibition (0)
g
Enterococcus 00009
Agosti
d
faecalis or 00087
Blue green col-
b
Specificity Listeria innocua 00017 — Qualitative onies without
opaque halo
a
Refer to the reference strain catalogue on http:// www .wfcc .info for information on culture collection strain numbers and
contact details. WDCM: World Data Centre for Microorganisms.
b
Strains to be used as a minimum.
c
Growth is categorized as 0: no growth; 1: weak growth (partial inhibition); 2: good growth (see ISO 11133).
d
Strain free of choice; one of the strains has to be used as a minimum.
e
Strain optional.
f
Half-Fraser broth is preferably tested with the volume used in the analysis (e.g. 225 ml).
g
In case of both quantitative and qualitative use for the medium, only results of the quantitative tests are required.
Table B.2 — Performance testing for the quality assurance of the culture media and reagents
for the confirmation tests
Medium or Function Incubation Control WDCM Reference Method of Criteria and
reagent strains number- media control characte
...