ASTM D6363-98(2013)

Standard Test Method for Determination of Hydrogen Peroxide and Combined Organic Peroxides in Atmospheric Water Samples by Peroxidase Enzyme Fluorescence Method

SIGNIFICANCE AND USE
5.1 Hydrogen peroxide (formed photochemically in the atmosphere) is a primary oxidizer of dissolved sulfur dioxide in atmospheric water. Detection of H2O2 in atmospheric water is useful for inferring gas-phase H2O2 concentrations and for assessing the relative importance of various acidifying mechanisms under specific atmospheric conditions.  
5.2 Hydroperoxides in samples to be analyzed are unstable in water and can decay rapidly due to bacterial action or chemical reaction with other constituents. The test method includes procedures for sample derivatization and methods for estimating and correcting for hydroperoxide decay.
SCOPE
1.1 This test method covers the determination of hydroperoxides, which include hydrogen peroxide (H2O2) and combined organic peroxides, in samples of atmospheric water by the method of horseradish peroxidase derivatization and fluorescence analysis of the derived dimer.2,3  
1.2 The range of applicable hydrogen peroxide concentrations was determined to be 0.6 - 176.0 × 10−6 M from independent laboratory tests of the test method.  
1.3 The primary use of the test method is for hydrogen peroxide, but it may also be used to quantitate organic hydroperoxides. Determinations of organic hydroperoxide concentration levels up to 30 × 10−6 M may be adequately obtained by calibration with hydrogen peroxide.2,3 While organic hydroperoxides have not been detected at significant concentration levels in rain or cloud water, their presence may be tested by operation of the test method with the addition of catalase for destruction of H2O23.  
1.4 Because of the instability of hydroperoxides in atmospheric water samples, proper sample collection, at-collection derivatization, and stringent quality control are essential aspects of the analytical process.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

General Information

• Status: Historical
• Publication Date: 30-Sep-2013
• ICS: 13.060.50 - Examination of water for chemical substances
• Technical Committee: D22 - Air Quality
• Drafting Committee: D22.03 - Ambient Atmospheres and Source Emissions

Standards Content (Sample)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: D6363 − 98 (Reapproved 2013)
Standard Test Method for
Determination of Hydrogen Peroxide and Combined Organic
Peroxides in Atmospheric Water Samples by Peroxidase
1
Enzyme Fluorescence Method
This standard is issued under the fixed designation D6363; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom-
1.1 This test method covers the determination of
mendations issued by the World Trade Organization Technical
hydroperoxides, which include hydrogen peroxide (H O ) and
2 2
Barriers to Trade (TBT) Committee.
combined organic peroxides, in samples of atmospheric water
by the method of horseradish peroxidase derivatization and
2. Referenced Documents
2,3
fluorescence analysis of the derived dimer.
4
2.1 ASTM Standards:
1.2 The range of applicable hydrogen peroxide concentra-
D1129Terminology Relating to Water
−6
tions was determined to be 0.6–176.0 × 10 M from indepen-
D1193Specification for Reagent Water
dent laboratory tests of the test method.
D1356Terminology Relating to Sampling and Analysis of
1.3 The primary use of the test method is for hydrogen Atmospheres
D5012Guide for Preparation of Materials Used for the
peroxide, but it may also be used to quantitate organic
hydroperoxides.Determinationsoforganichydroperoxidecon- Collection and Preservation of Atmospheric Wet Deposi-
−6
tion
centration levels up to 30 × 10 M may be adequately
2,3
obtained by calibration with hydrogen peroxide. While D5111Guide for Choosing Locations and Sampling Meth-
ods to Monitor Atmospheric Deposition at Non-Urban
organic hydroperoxides have not been detected at significant
concentration levels in rain or cloud water, their presence may Locations
E200Practice for Preparation, Standardization, and Storage
be tested by operation of the test method with the addition of
3
catalase for destruction of H O . of Standard and Reagent Solutions for ChemicalAnalysis
2 2
1.4 Because of the instability of hydroperoxides in atmo-
3. Terminology
spheric water samples, proper sample collection, at-collection
3.1 Definitions—For definitions of terms used in this test
derivatization, and stringent quality control are essential as-
method, refer to Terminologies D1129 and D1356 and Guide
pects of the analytical process.
D5111.
1.5 This standard does not purport to address all of the
3.2 Definitions of Terms Specific to This Standard:
safety concerns, if any, associated with its use. It is the
3.2.1 atmospheric water, n—liquidorsolidwatersuspended
responsibility of the user of this standard to establish appro-
in the atmosphere or deposited from the atmosphere. Forms of
priate safety and health practices and determine the applica-
atmospheric water include rain, snow, fog, cloud water, dew,
bility of regulatory limitations prior to use.
and frost.
1.6 This international standard was developed in accor-
dance with internationally recognized principles on standard- 3.2.2 derivatization, n—formation of the
p-hydroxyphenylacetic acidic dimer by combination of
p-hydroxyphenylacetic acid, horseradish peroxidase reagent,
1
This guide is under the jurisdiction of ASTM Committee D22 on Air Quality
and is the direct responsibility of Subcommittee D22.03 on Ambient Atmospheres and hydroperoxide(s). Also the procedure of addition of the
and Source Emissions.
derivatizing reagent to samples.
Current edition approved Oct. 1, 2013. Published October 2013. Originally
ε1
3.2.3 hydroperoxides, n—hydrogen peroxide and organic
approved in 1998. Last previous edition approved in 2009 as D6363–98 (2009) .
DOI: 10.1520/D6363-98R13.
peroxides dissolved in water.
2
Lazrus, A. L., Kok, G. L., Gitlin, S. N., and Lind, J. A., “Automated
Fluorometric Method for Hydrogen Peroxide in Atmospheric Precipitation,” Anal.
4
Chem., 57, 1985, pp. 917–922. For referenced ASTM standards, visit the ASTM website, www.astm.org, or
3
Kok,G.L.,Thompson,K.,andLazrus,A.L.,“DerivatizationTechniqueforthe contactASTM Customer Service at [e-mail]. ForAnnual Book ofASTM
Determination of Peroxides in Precipitation,” Anal. Chem., 58, 1986, pp. Standards volume information, refer to the standard’s Document Summary page on
1192–1194. the ASTM website.
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D6363 − 98 (2013)
3.2.4 intrinsic hydroperoxides, n—hydroperoxides con- components of precipitation have demonstrated both the gen-
tained in r
...